Status
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Gene information
General information
TC032051
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000755
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5912290..5916407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032051
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09611

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032051 RA
      MKFCFVTFL SLFLTRGTL QTCLVTNFI TGIRIYGMP 
      QRDKCIQCQ NEVISSELT AKVKSLVDN WRAKRLEIT 
      DTNSPDVPA GVFNGLYYL QALCINNAS VETIQPEAF 
      TGLNFLSQL SLKSNKIEF VEEDWFANL TVLEDLDLS 
      FNKIASIHN KTFDSLTQL RVLSLRNNF LTELPPDLF 
      KGQNLRLQV LDLSFNKLT TIENNRFIT TSQTSCCLV 
      RVYCTMKSV FCTTFFLIV LIKESIPLC VVVKIGPPR 
      PNYGYWPTT EAIQCRNEV ISSDLAEKV LKLTEMHPS 
      HSLEIIDGN NPEIPADGF KLLRSLPAL RINNASVRT 
      IQPGAFNGL RYLKQLSLK YNRIEVLNE NCFENLTML 
      TDLDLSFNK IVSIHSKSF ANLTRLRVL FLQNNLIAE 
      LPPDLFRDQ NYWLRVVDL SFNKLLTVK KQLLFDLTD 
      LYTIYLNNN RINHLESDV FNTKKLKHI HIDNNELQS 
      LTNLTLPNT VELLNVSHN LIKSMSFSK YVVISLLDL 
      SANGLASAN GLKLENATI KQLNLDQNN FGTYPSVNQ 
      TAFFSNLIA LETLSLSNN NLQQLSRNS FTHNQHLTK 
      LNLTGNSLR NGDFLNFLP NLQYLNVSF NNFDSNTNL 
      SVLISLKNL DFSFNSLDQ IRYNFLGGL KFLQTLNLS 
      HNKISKISI GCFRDLQML YILDLSNNE ISSLELGVF 
      GGLTRLSEL DLSHNGLIQ LNEGTFHNF KYLHVLNLR 
      KTGLSYTVV ENIMSHFPG LINIDLAEN NWSCTLLIK 
      FVKKHASVR LLGGRDYNT ANVHGVACN DQVEDTTEL 
      VNTSENFPD FRVTNTLLI FILIVLIVQ LASKHISVF 
      WKRKKGQKQ ESDQELRLV SEC
    CDS sequence
    • >TC032051 RA
      atgaagttt tgttttgtc acattcctc tctttattc 
      ttaacaaga gggactctg caaacttgt cttgtcacg 
      aattttata acaggcata cgcatctat ggaatgccg 
      caaagagac aaatgcatt cagtgtcag aacgaagtt 
      atttcctcc gaactaact gcaaaagta aaatcactt 
      gttgacaac tggagagca aagcgctta gaaatcact 
      gataccaac agtcctgac gtacctgca ggtgttttc 
      aacggactt tactacctc caagcatta tgtattaac 
      aacgccagt gtggaaact atccaaccg gaagccttc 
      actggactg aattttttg tcgcaactg tcgttaaaa 
      tccaacaaa attgaattt gtggaggaa gattggttt 
      gcaaactta actgtgctg gaagattta gatttgagt 
      tttaataag attgcaagt attcacaac aaaactttc 
      gatagttta acacaactg cgtgtactt tcattgcgg 
      aataatttt ctcactgaa ttaccacct gacttgttt 
      aagggccag aatctgaga ctgcaagtt ttagatctg 
      tcgtttaac aagttgaca acgatcgaa aataatcga 
      ttcatcaca acaagtcaa acaagttgc tgtttagtt 
      cgtgtttat tgcacaatg aaatctgta ttctgcaca 
      acgttcttt ttgattgtg ttaattaaa gaaagtatt 
      ccattgtgc gttgtggtc aaaatcggg ccacctagg 
      ccaaattac ggctattgg ccaaccacg gaagccatt 
      cagtgtcga aatgaagtc atttcttcc gatttagct 
      gaaaaagtg ctgaaactc actgaaatg cacccgtca 
      cattcttta gaaataatc gacggaaat aatcctgaa 
      atacctgca gatgggttc aaacttctt cgcagtctt 
      cctgctttg cgaattaac aacgccagt gtacgcact 
      atccaaccg ggagctttc aatgggttg agatatttg 
      aagcaactg tcgttaaaa tacaacaga attgaggtt 
      ctgaatgaa aactgtttt gaaaacttg acgatgttg 
      actgatttg gatttaagt tttaataag attgtgtct 
      attcacagc aaaagtttc gctaattta acgagactg 
      cgtgtgctt ttcttgcaa aataatctt attgctgag 
      ttgccacct gacttgttt agagaccag aattactgg 
      ctacgtgtt gtggacttg tcatttaac aagttgtta 
      actgttaaa aagcaacta ctttttgat cttacagac 
      ttgtacact atatattta aacaacaat agaataaac 
      cacttagag agcgacgtg tttaacacg aaaaaattg 
      aaacacatt cacattgat aataatgaa ctgcaatcg 
      ttgaccaac ttaactttg ccaaataca gttgaattg 
      ttaaatgta agtcataat ttaatcaag tcgatgagt 
      ttttccaaa tacgttgta atttcttta cttgacttg 
      agtgcaaat ggacttgcg tctgcaaac ggtttgaag 
      cttgaaaat gcaacaatt aaacaatta aacttggat 
      caaaacaac tttggtaca tatccaagt gtaaaccaa 
      actgcgttt ttcagtaat ttaatagca ttagaaact 
      ctttctctc agtaataat aacttgcaa caattaagt 
      cgtaactca ttcacgcac aatcaacat ttaacaaaa 
      ttaaatctc acaggaaat tctttacgt aacggtgat 
      tttttaaat ttccttcct aatttgcaa tacctaaat 
      gttagcttc aacaacttt gattctaac acaaatctc 
      agtgtttta atatccctg aaaaacctt gattttagt 
      ttcaatagc ttggatcaa attcggtat aattttctt 
      ggcggtttg aagttctta caaacgtta aatctgagt 
      cataacaaa atttcaaaa atcagtata ggttgcttc 
      agagatttg caaatgctg tacatactt gatttatca 
      aacaatgaa atttcatcc ttggagctt ggtgttttt 
      gggggattg acccgcttg tccgaactc gatctgtcc 
      cacaacgga ttaattcag ttgaacgaa ggaactttt 
      cacaacttc aaatatttg catgttctt aacttacga 
      aaaactggt ttgagttac acagttgtc gaaaacata 
      atgtcgcat tttcctgga cttataaac atagatttg 
      gctgaaaat aactggtct tgcaccctt ttgattaaa 
      tttgtaaaa aaacatgcc tcagtaaga cttttaggt 
      ggaagggat tataacaca gcaaatgta cacggagtt 
      gcttgcaat gatcaagtc gaagatacc acggaatta 
      gtaaacaca tcagagaat tttcctgat tttagggtc 
      acaaacact ttattaatt tttatactg atagtttta 
      atcgtacaa ttggcgtct aaacatatt tccgtgttt 
      tggaaaaga aaaaaaggc cagaagcaa gaaagtgat 
      caagagttg cgtctcgtt tccgagtgt tag
    mRNA sequence
    • >TC032051 RA
      caaaaactg aacgtgtcc ataatgaag ttttgtttt 
      gtcacattc ctctcttta ttcttaaca agagggact 
      ctgcaaact tgtcttgtc acgaatttt ataacaggc 
      atacgcatc tatggaatg ccgcaaaga gacaaatgc 
      attcagtgt cagaacgaa gttatttcc tccgaacta 
      actgcaaaa gtaaaatca cttgttgac aactggaga 
      gcaaagcgc ttagaaatc actgatacc aacagtcct 
      gacgtacct gcaggtgtt ttcaacgga ctttactac 
      ctccaagca ttatgtatt aacaacgcc agtgtggaa 
      actatccaa ccggaagcc ttcactgga ctgaatttt 
      ttgtcgcaa ctgtcgtta aaatccaac aaaattgaa 
      tttgtggag gaagattgg tttgcaaac ttaactgtg 
      ctggaagat ttagatttg agttttaat aagattgca 
      agtattcac aacaaaact ttcgatagt ttaacacaa 
      ctgcgtgta ctttcattg cggaataat tttctcact 
      gaattacca cctgacttg tttaagggc cagaatctg 
      agactgcaa gttttagat ctgtcgttt aacaagttg 
      acaacgatc gaaaataat cgattcatc acaacaagt 
      caaacaagt tgctgttta gttcgtgtt tattgcaca 
      atgaaatct gtattctgc acaacgttc tttttgatt 
      gtgttaatt aaagaaagt attccattg tgcgttgtg 
      gtcaaaatc gggccacct aggccaaat tacggctat 
      tggccaacc acggaagcc attcagtgt cgaaatgaa 
      gtcatttct tccgattta gctgaaaaa gtgctgaaa 
      ctcactgaa atgcacccg tcacattct ttagaaata 
      atcgacgga aataatcct gaaatacct gcagatggg 
      ttcaaactt cttcgcagt cttcctgct ttgcgaatt 
      aacaacgcc agtgtacgc actatccaa ccgggagct 
      ttcaatggg ttgagatat ttgaagcaa ctgtcgtta 
      aaatacaac agaattgag gttctgaat gaaaactgt 
      tttgaaaac ttgacgatg ttgactgat ttggattta 
      agttttaat aagattgtg tctattcac agcaaaagt 
      ttcgctaat ttaacgaga ctgcgtgtg cttttcttg 
      caaaataat cttattgct gagttgcca cctgacttg 
      tttagagac cagaattac tggctacgt gttgtggac 
      ttgtcattt aacaagttg ttaactgtt aaaaagcaa 
      ctacttttt gatcttaca gacttgtac actatatat 
      ttaaacaac aatagaata aaccactta gagagcgac 
      gtgtttaac acgaaaaaa ttgaaacac attcacatt 
      gataataat gaactgcaa tcgttgacc aacttaact 
      ttgccaaat acagttgaa ttgttaaat gtaagtcat 
      aatttaatc aagtcgatg agtttttcc aaatacgtt 
      gtaatttct ttacttgac ttgagtgca aatggactt 
      gcgtctgca aacggtttg aagcttgaa aatgcaaca 
      attaaacaa ttaaacttg gatcaaaac aactttggt 
      acatatcca agtgtaaac caaactgcg tttttcagt 
      aatttaata gcattagaa actctttct ctcagtaat 
      aataacttg caacaatta agtcgtaac tcattcacg 
      cacaatcaa catttaaca aaattaaat ctcacagga 
      aattcttta cgtaacggt gatttttta aatttcctt 
      cctaatttg caataccta aatgttagc ttcaacaac 
      tttgattct aacacaaat ctcagtgtt ttaatatcc 
      ctgaaaaac cttgatttt agtttcaat agcttggat 
      caaattcgg tataatttt cttggcggt ttgaagttc 
      ttacaaacg ttaaatctg agtcataac aaaatttca 
      aaaatcagt ataggttgc ttcagagat ttgcaaatg 
      ctgtacata cttgattta tcaaacaat gaaatttca 
      tccttggag cttggtgtt tttggggga ttgacccgc 
      ttgtccgaa ctcgatctg tcccacaac ggattaatt 
      cagttgaac gaaggaact tttcacaac ttcaaatat 
      ttgcatgtt cttaactta cgaaaaact ggtttgagt 
      tacacagtt gtcgaaaac ataatgtcg cattttcct 
      ggacttata aacatagat ttggctgaa aataactgg 
      tcttgcacc cttttgatt aaatttgta aaaaaacat 
      gcctcagta agactttta ggtggaagg gattataac 
      acagcaaat gtacacgga gttgcttgc aatgatcaa 
      gtcgaagat accacggaa ttagtaaac acatcagag 
      aattttcct gattttagg gtcacaaac actttatta 
      atttttata ctgatagtt ttaatcgta caattggcg 
      tctaaacat atttccgtg ttttggaaa agaaaaaaa 
      ggccagaag caagaaagt gatcaagag ttgcgtctc 
      gtttccgag tgttagtgc aatttaaaa taaatttat 
      atatattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY