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Gene information
General information
TC000767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5726841..5729168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000767
    iBeetle screen iB_09607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000767 RA
      MKHPSGDTS KAHMLKYLS LFVLTVQNA LLGLSMRYA 
      RTRDGDMFL SSTAVLMAE VVKLVTCLI IVYAESGGI 
      VQLFDAIDK QIIKQPIDT LKVCVPSFV YVIQNNLLY 
      VSASHLDAA TYQVTYQLK ILTTAMFAV LILKKELLK 
      TQWVSLVLL VIGVVLVQL AQSPNSAQV HTGPKQNRF 
      IGFMAAVSA CVLSGFAGV FFEKMLKGS DITVWMRNV 
      QLSVCSIPF ALISCFAYD GNVISQRGI FFGYDKFVW 
      YLVLLQACG GLIVAVVVK FADNILKGF ATSLAIVIS 
      CIASIYIFD FQLTAQFTL GAAFVIGSI FLYGHTPKK 
      STPMSAHKV
    CDS sequence
    • >TC000767 RA
      atgaaacat ccctcaggt gacacgtcc aaagctcac 
      atgctgaaa tatctgagc ttgtttgtt ttaaccgtt 
      caaaatgct ttattaggc ctcagtatg cgatatgct 
      aggactagg gatggggac atgttctta tcatcaaca 
      gctgttctt atggcagag gtagtgaaa ttagtgact 
      tgcttaata atcgtttac gccgaaagt ggtggaatc 
      gttcaactt ttcgatgca atcgacaaa caaatcatc 
      aaacagccc atcgacacc ttaaaagtg tgtgtccct 
      tcgtttgta tacgtcata caaaataat ctcctttat 
      gtatctgcg tcacatttg gacgcagca acatatcag 
      gtcacttat caattgaag attctaaca acggcaatg 
      tttgccgtc ttgatccta aaaaaagaa cttttgaaa 
      acacagtgg gtatccttg gttttgttg gtcattggt 
      gtagtcctt gtccaactg gcccaaagt ccgaacagt 
      gcacaagtc cacacaggg cctaaacag aaccgtttc 
      attggtttt atggccgct gtttcggct tgtgttctt 
      tcgggcttc gctggggtt tttttcgaa aaaatgctc 
      aaaggtagt gatataacg gtgtggatg cgtaatgtg 
      cagttaagt gtttgttcc attcctttt gcgctaatt 
      tcatgtttt gcgtacgat gggaatgtt atttcccag 
      cgagggatt tttttcgga tatgataag ttcgtctgg 
      tatcttgtt ttgttgcaa gcttgtggc gggttaatt 
      gttgctgtt gttgttaaa ttcgcggat aatatttta 
      aaagggttc gctacttcc ttagctatt gttatctca 
      tgtatcgct tctatctat atctttgac ttccaactc 
      acagcgcag tttacgcta ggagcggct tttgtgatt 
      gggtccata tttttgtat ggacatact ccgaaaaaa 
      agtacccca atgtcggcg cataaggta taa
    mRNA sequence
    • >TC000767 RA
      tacgttttt gaaaacagg gtaaaatcc ttgtagttg 
      tggcatgca atagtttaa aactgtgcg aatttcaaa 
      catgcaaat tttggcgcc attttcgat gacacctac 
      cgctaccgc tttgacgtg tcatgtcat cgtaaaatt 
      gctacaatt tttaaatga aatgtctcg tattttacc 
      tcttatctt taatatcgc gttacacaa ttaataatc 
      gattagttt aagccgata atataatag atttttaat 
      gcttcgaac acataccag gaacttaga ctgattcaa 
      cactaatac acattttta ttacgatag ctgacacta 
      gccacaact atgaaacat ccctcaggt gacacgtcc 
      aaagctcac atgctgaaa tatctgagc ttgtttgtt 
      ttaaccgtt caaaatgct ttattaggc ctcagtatg 
      cgatatgct aggactagg gatggggac atgttctta 
      tcatcaaca gctgttctt atggcagag gtagtgaaa 
      ttagtgact tgcttaata atcgtttac gccgaaagt 
      ggtggaatc gttcaactt ttcgatgca atcgacaaa 
      caaatcatc aaacagccc atcgacacc ttaaaagtg 
      tgtgtccct tcgtttgta tacgtcata caaaataat 
      ctcctttat gtatctgcg tcacatttg gacgcagca 
      acatatcag gtcacttat caattgaag attctaaca 
      acggcaatg tttgccgtc ttgatccta aaaaaagaa 
      cttttgaaa acacagtgg gtatccttg gttttgttg 
      gtcattggt gtagtcctt gtccaactg gcccaaagt 
      ccgaacagt gcacaagtc cacacaggg cctaaacag 
      aaccgtttc attggtttt atggccgct gtttcggct 
      tgtgttctt tcgggcttc gctggggtt tttttcgaa 
      aaaatgctc aaaggtagt gatataacg gtgtggatg 
      cgtaatgtg cagttaagt gtttgttcc attcctttt 
      gcgctaatt tcatgtttt gcgtacgat gggaatgtt 
      atttcccag cgagggatt tttttcgga tatgataag 
      ttcgtctgg tatcttgtt ttgttgcaa gcttgtggc 
      gggttaatt gttgctgtt gttgttaaa ttcgcggat 
      aatatttta aaagggttc gctacttcc ttagctatt 
      gttatctca tgtatcgct tctatctat atctttgac 
      ttccaactc acagcgcag tttacgcta ggagcggct 
      tttgtgatt gggtccata tttttgtat ggacatact 
      ccgaaaaaa agtacccca atgtcggcg cataaggta 
      taaagacaa ccataagcg atttatttt tacttatac 
      atttgaatt ttttagtcg taatcttat tttaacgct 
      tgcagcatc tcaattttt tattattaa gtttcgtaa 
      gcgggccat ttgttatgt attgtcttt cttatttta 
      tatacttgt gatgttcgt attctgatc ttattgtat 
      aatttattg aagtagtat tgactttcg tacttaatt 
      taacctcat aatgtttag tgcaataag tgaaaataa 
      aatattttt gtaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY