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Gene information
General information
TC000793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5720250..5726805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000793
    iBeetle screen iB_09606

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 1
    yolk late sometimesamount decreased - Number of animals showing the phenotype: 1

    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000793 RA
      MPEEEKNQV LPIALGVGV IITSVLIYH FYKTSKPSK 
      SKGKKKILL EDPQAKYSL PLIEKEEIS HDTRRFRFG 
      LPTKEHVLG LPIGQHIHL SAKIGDDLI IRSYTPVSS 
      DDDHGFVDL VVKVYFKKV HPRFPDGGK MTQHLESLK 
      IGDTIEVRG PSGRLQYQG HGTFSIKKL RKDPPQIVT 
      VNKVSMIAG GTGITPMLQ LIRHITKDP SDKTKLKLI 
      FANQTEKDI LVRKELEEV AKEHPDQFE LWYTLDTPP 
      ENWKYSSGF INENMIKDH LFPPSNDNI VLMCGPPPM 
      INYACNPSL EKLKYDKDL CFAY
    CDS sequence
    • >TC000793 RA
      atgccggaa gaagaaaaa aaccaggtt ctaccaata 
      gccctaggg gtcggcgtc atcatcaca agtgtatta 
      atttatcac ttctacaaa acatcaaaa ccatcaaaa 
      tcaaaaggc aaaaagaag attttactt gaggaccct 
      caagcgaaa tattcgctt cctttgatt gaaaaggaa 
      gaaatcagt catgatacc cgcagattt aggtttggg 
      ttgcccaca aaagagcac gttttgggg cttcccatt 
      ggtcaacat attcatttg tctgcaaaa atcggtgat 
      gacttaata atccggtca tacaccccc gtatcttca 
      gacgatgac catggattt gttgactta gtggtcaag 
      gtgtacttc aaaaaagtc cacccccga tttcccgat 
      gggggcaaa atgacgcaa cacctcgaa agtttgaaa 
      atcggtgat accatcgaa gttagagga ccatctgga 
      cggttacag taccaagga catgggacc ttttccatt 
      aaaaaacta cgcaaagac ccaccacaa atcgttaca 
      gttaacaaa gtttctatg attgcgggt ggtactggc 
      attacaccc atgctacaa cttatcaga cacatcaca 
      aaagaccca agtgataag acaaaacta aaactgatt 
      tttgcaaat cagactgag aaagacatt ttggttcga 
      aaggaactg gaggaagtg gcgaaggag catccagat 
      cagtttgaa ctgtggtac actctcgat acacctcca 
      gaaaattgg aaatacagt tcaggcttt atcaatgaa 
      aatatgatc aaggaccat ctcttcccc ccaagcaat 
      gataacatt gtactcatg tgtggaccc ccacctatg 
      attaattac gcctgtaat cccagtttg gagaagttg 
      aaatatgat aaagatctg tgcttcgct tattaa
    mRNA sequence
    • >TC000793 RA
      gcctggtta ggaaataaa tggtattac aggaaactg 
      tcatgtgtt tcttttgtt ctatttgga aaatttttc 
      aaaaaagca cagccatcc ttgatgtta atttttaca 
      aagccagat tttgtaaaa aaaattggt tttgtttag 
      ttaaaaatt gagttcttt caaggtgta cgatttcaa 
      gtcatattt aaaagctcc agacaacct tctaaatat 
      aaagtaaaa tattttttg ggcgcattc accggtcgt 
      tctgttgaa cagaaacag aacatactt ctcgttcgc 
      gtctggcat gtgtttggt ggtgtgata tcatttttg 
      agataaaga tttttacat aatagttat tatagaaca 
      aagatgaca aacgtattg agtggggta agtgattat 
      ttgcagttt acgtaatga taattcagt cgaataaag 
      gctgaaggc gtagtttat tgatagtgt ggtcagtta 
      ccaactttg ttcaaagta cgaggtgac aatgccgga 
      agaagaaaa aaaccaggt tctaccaat agccctagg 
      ggtcggcgt catcatcac aagtgtatt aatttatca 
      cttctacaa aacatcaaa accatcaaa atcaaaagg 
      caaaaagaa gattttact tgaggaccc tcaagcgaa 
      atattcgct tcctttgat tgaaaagga agaaatcag 
      tcatgatac ccgcagatt taggtttgg gttgcccac 
      aaaagagca cgttttggg gcttcccat tggtcaaca 
      tattcattt gtctgcaaa aatcggtga tgacttaat 
      aatccggtc atacacccc cgtatcttc agacgatga 
      ccatggatt tgttgactt agtggtcaa ggtgtactt 
      caaaaaagt ccacccccg atttcccga tgggggcaa 
      aatgacgca acacctcga aagtttgaa aatcggtga 
      taccatcga agttagagg accatctgg acggttaca 
      gtaccaagg acatgggac cttttccat taaaaaact 
      acgcaaaga cccaccaca aatcgttac agttaacaa 
      agtttctat gattgcggg tggtactgg cattacacc 
      catgctaca acttatcag acacatcac aaaagaccc 
      aagtgataa gacaaaact aaaactgat ttttgcaaa 
      tcagactga gaaagacat tttggttcg aaaggaact 
      ggaggaagt ggcgaagga gcatccaga tcagtttga 
      actgtggta cactctcga tacacctcc agaaaattg 
      gaaatacag ttcaggctt tatcaatga aaatatgat 
      caaggacca tctcttccc cccaagcaa tgataacat 
      tgtactcat gtgtggacc cccacctat gattaatta 
      cgcctgtaa tcccagttt ggagaagtt gaaatatga 
      taaagatct gtgcttcgc ttattaagt caaatccaa 
      tggaactgg atattgttt ttacgatta gttgtttta 
      ggtttttat gactgttat ccctggttg ttgttaatt 
      ccagtatga tgtaataat acactgttg acttttgtg 
      aacaataaa cttatattc caata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY