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Gene information
General information
TC004977 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5433757..5448499
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004977
    iBeetle screen iB_09602

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004977 RA
      MSTTESWKE EEGNGSFQG FDYGLLVPI SSQTPVVVD 
      SGENFMPIW VKVLWTVIF MVMILIATG GNCIVIWIV 
      TAHRRMRTV TNYFLVNLS LADLLLTTF NCIFNFSYM 
      IQRDWPFGS LYCIISNFI ANATVAASV FTLTGISCD 
      RYLAIVHPL QPRMSKRAS LITITFIWL ASMTVAFPC 
      LLYSTTITN KYKGVERTG CILIWPDGK VVGSHFDFA 
      YQMFFLIIT YVIPVTLMS FSYTIMGKE LWGSRSIGE 
      MTQRQIDSI RSKRKVVKM FIFVVFIFA ICWLPYHGY 
      FLYVYYDTD IIFSKYTQH VYLAFYWFA MSNAMVNPL 
      IYYWMNARF RQYFKTAIC GWKECILHK KCQGDDSSL 
      HTECRQSHS GFYRSRKSK REDKLGADS PNNVASPTT 
      KTHEFNETF KAKNNQRWV RSSFRNDSK RTTVNL
    CDS sequence
    • >TC004977 RA
      atgagtaca actgaaagt tggaaagag gaagaaggt 
      aacggaagc ttccaagga tttgattac gggcttctc 
      gtacctatt tccagccaa acccctgtt gttgtcgac 
      tctggggaa aatttcatg cccatatgg gtgaaagtg 
      ctatggacg gtgattttc atggtcatg attttaatc 
      gcaacggga ggaaattgt atagtcatt tggattgtc 
      accgctcat cgcaggatg aggacagtg actaattac 
      ttccttgtt aatttaagt ttagccgac ttgcttcta 
      acgacgttc aattgcatt tttaatttc tcctacatg 
      attcaaaga gattggccg tttggaagt ctttattgc 
      attattagc aactttatt gcaaacgct acggtagca 
      gccagcgtg tttaccctg actggaatt tcctgcgac 
      agatactta gcaatcgtc catcctctt cagccacga 
      atgtccaaa cgggcctca cttatcacc attacgttt 
      atttggctg gccagcatg acagtggcc ttcccctgt 
      ctcctgtac tcaaccaca atcacaaat aagtataaa 
      ggggtggaa agaacaggc tgcatcctc atttggcca 
      gacggtaaa gtcgtcggc tcccatttc gactttgcg 
      taccagatg ttttttcta ataataact tacgtaatc 
      cccgtcaca ttgatgtct ttttcctac accattatg 
      gggaaggag ctttggggc tcgcgctcc attggggag 
      atgacacaa agacagatc gactcgatt cggtcgaaa 
      agaaaggtg gtgaaaatg ttcatattc gtggttttt 
      atctttgcg atttgctgg ctcccctac cacggctat 
      tttctgtat gtttactac gacacggat ataatattc 
      agtaaatat actcaacac gtttatctg gcattctac 
      tggttcgca atgtcgaac gctatggta aatccattg 
      atatattat tggatgaat gctagattc cgtcaatac 
      tttaaaaca gcgatatgt ggctggaag gagtgtatt 
      ttgcacaaa aaatgtcaa ggagacgac tcgtccctt 
      cacacagag tgtcgacaa tcgcactcg ggtttttat 
      aggtcgagg aaatcaaaa cgagaggac aaactagga 
      gcagatagt cccaacaac gttgcttca ccaacaacg 
      aaaacccac gaatttaat gaaactttt aaagcgaaa 
      aataaccaa agatgggtg cgatcgtcg tttcggaac 
      gatagtaaa aggacgact gttaattta tag
    mRNA sequence
    • >TC004977 RA
      ttgccataa tttggccaa aggataagg tcgtaaagc 
      aagttacaa gaactcgcg gacaattgt tgctcgcga 
      ttcagactc aagttattt ccgttacta aacgtgaac 
      gtgtgtttc aagtttaat tggtgataa gtgtattac 
      aatgcctca agtggagga taattccgg agtaatctt 
      tgtcgctta aaagttaga aatgagtac aactgaaag 
      ttggaaaga ggaagaagg taacggaag cttccaagg 
      atttgatta cgggcttct cgtacctat ttccagcca 
      aacccctgt tgttgtcga ctctgggga aaatttcat 
      gcccatatg ggtgaaagt gctatggac ggtgatttt 
      catggtcat gattttaat cgcaacggg aggaaattg 
      tatagtcat ttggattgt caccgctca tcgcaggat 
      gaggacagt gactaatta cttccttgt taatttaag 
      tttagccga cttgcttct aacgacgtt caattgcat 
      ttttaattt ctcctacat gattcaaag agattggcc 
      gtttggaag tctttattg cattattag caactttat 
      tgcaaacgc tacggtagc agccagcgt gtttaccct 
      gactggaat ttcctgcga cagatactt agcaatcgt 
      ccatcctct tcagccacg aatgtccaa acgggcctc 
      acttatcac cattacgtt tatttggct ggccagcat 
      gacagtggc cttcccctg tctcctgta ctcaaccac 
      aatcacaaa taagtataa aggggtgga aagaacagg 
      ctgcatcct catttggcc agacggtaa agtcgtcgg 
      ctcccattt cgactttgc gtaccagat gttttttct 
      aataataac ttacgtaat ccccgtcac attgatgtc 
      tttttccta caccattat ggggaagga gctttgggg 
      ctcgcgctc cattgggga gatgacaca aagacagat 
      cgactcgat tcggtcgaa aagaaaggt ggtgaaaat 
      gttcatatt cgtggtttt tatctttgc gatttgctg 
      gctccccta ccacggcta ttttctgta tgtttacta 
      cgacacgga tataatatt cagtaaata tactcaaca 
      cgtttatct ggcattcta ctggttcgc aatgtcgaa 
      cgctatggt aaatccatt gatatatta ttggatgaa 
      tgctagatt ccgtcaata ctttaaaac agcgatatg 
      tggctggaa ggagtgtat tttgcacaa aaaatgtca 
      aggagacga ctcgtccct tcacacaga gtgtcgaca 
      atcgcactc gggttttta taggtcgag gaaatcaaa 
      acgagagga caaactagg agcagatag tcccaacaa 
      cgttgcttc accaacaac gaaaaccca cgaatttaa 
      tgaaacttt taaagcgaa aaataacca aagatgggt 
      gcgatcgtc gtttcggaa cgatagtaa aaggacgac 
      tgttaattt atagttttg aggactgtg gaaatgtgc 
      tcaggatag taaaaagtg gttgtaatt tattaaatg 
      tcttatttt atttactat gattaattg ttgttgcat 
      tgttatgac attgacttg aatgtaaat tctgtgata 
      tttgataac aataacaaa aatattttt taaataaaa 
      tttaatgcc ctag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY