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Gene information
General information
TC004424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5354181..5373341
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004424
    iBeetle screen iB_09601

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004424 RA
      MGFLSSWRL GPISVFLAI CCICSINCQ NLGPSNTPE 
      AYFNGSAYL RLQTTISPI KQTGLSFRT CTGGGLFSQ 
      QQNDDFLEV SVNSEGVIF FAKTAGKQF NNKIFGNFI 
      NNKWHVVFL YYEQGNLTL RVDNEKQLV ANSSYQTEL 
      ITNPGFYNE GASVLLVGK QFNGCLLEG PSLVFNHSI 
      IYNHNVKFE PCPIPEDSC ILQVIDYCV TEPCMRRGT 
      CHNSPKGYN CTCQPRYTG KNCEMDLGN PCERNPAIC 
      KNGATCNAD NTGVYTCTC PPNFTGKHC EQLIVVNQQ 
      CQDNPCQNG ATCVSNGNM ECLCLPGFD GPKCEFNID 
      DCKGNPCKN GGICRDGLD NFTCDCSRT GYTGRFCQI 
      NINECETSP CLNHGTCFD TYGGFLCQC PPGYGGAYC 
      QNTLHACSS QQCLNEGQC INTPDGFKC ICPDGFAGE 
      RCEAGERQI SCDGTKCPP YADCVKAGN NFGCICKPE 
      YPGNYPNCS IPNICANNP CKNQGICTS WNGYFNCSC 
      SPGFTGQLC EIPVDSQPN CNSNPCQNG GSCFDKPTG 
      GFYCNCTDQ WMGTYCNES YDVCKLEPC QNNATCISS 
      QNKRDFVCE CLPGFEGQH CERNIDDCV GVTCPYGQV 
      CFDLVNDHE CRCPLGYKG ENCTIDADP CAKKPCMNG 
      ATCQMNHNE NGFVCNCLE GFSGERCET DIDECKNQP 
      GICNEGICQ NELGGFQCY CRPGYTGER CDLDFDECL 
      SMPCRHQAT CLNKVNNYE CICPPGYEG KDCSININE 
      CEPMPCMNG STCIDGINK FTCNCQPGL TGKICEINI 
      DDCESSPCL NGAECIDGL NSYTCNCTD TGYTGTHCE 
      TNINDCIGD PCENGASCE DKVKDYDCH CYAGYSGKN 
      CEIDINECD SNPCKHNGT CYEHSNQTL YQLSDQTLP 
      EIFSAEFSY DKAAGYECL CVDGVTGEN CEIDINECE 
      SNPCFEGTC VDKIGGFAC TCDEGFEGE RCDIDINEC 
      ERFKPCVYG KCWDRRASY YCECDANYG GKNCSVGLT 
      GCDTNPCLN SGTCKPYLI DENIHRFNC SCPHGYYGQ 
      VCEKITTMS LSGKSLIIV NTSREEGYD IQFRFKTTL 
      GDGLLALGK GLTYYILEL SRGRLNLHS SLLNKWEGV 
      FIGSNLNDS QWQRVFVAI NSTHLVLSA NDEQTIYPI 
      SFNEISNGT TYTSFPLTY IGGIPSNLK KLTHGKTYL 
      VGCTEDVLI NGEWILPPS SSPHTNPAF TFQEVEPSC 
      KRQPQCNPN PCHSGGHCT DLWVNFKCT CGRPYLGDT 
      CQYNYTAAT FGYENITDS LVTVYVDSA ARRAVRTIV 
      DISMFIRTR QPKGQIFYL GSLPRTMNP SEETYIAAQ 
      LEGGELLVR IQFNGTPEA YTVGGVKLD NGYDHLIEV 
      IRNVTLVQV KLNGTEYFR KTISATGTL DAQVLFLGG 
      PPQTRPVRQ ANDNLVKID TISPTSSAV IATPLSNVH 
      FKGIIQDVQ ISNGSSVMI VEFFPLNVK DLDIPKSFG 
      EVRFDETSV LEGVRSDNS CRINPCLHN GVCENTWND 
      YRCICTRGF KGKDCSDLE FCEIERCPK ESVCKNLED 
      GSECVANAT FDGKTPPLK YRLVTWPNS TKIVSYDTL 
      EVTYRTRSW GTIFFAKHE NDFFAIFIY HNEVVVEWS 
      INKMGDSKR FRKDYFEGQ WLTIYFEYK NSILKGGFK 
      DMVMDEAPN MKVANFDIE GFTRILTFG DIYVAGSDE 
      VTLDYQNII NSSNDNMTG YIPYSDTTT TPSTTSNSV 
      EENDFFSDV SLFKVDQNK STDFFKGCI NEIRIGGIL 
      LPFFPSNEL FQKYHYFEL YSENRPQIG CILCHPIDC 
      YNQGVCANP LEFYKCNCP AGFAADDCS IDINECEKN 
      ECQNNATCI DLVAEYECK CEPGFEGEH CETDIDECA 
      SNPCRHGGT CNDAIGTFK CECPEGFAG KQCEAPILI 
      TCDNKPCKE GATCKTGPN EITGNNFTC ICTPGMEGP 
      LCDTPFCYQ QPCINNGTC VTETEVPFC NCTVRGFEG 
      KFCEINIDD CDIPTGNPC QHGGICNDQ VNKYECDCN 
      GTGWGGLLC ENDINECVQ MPEPCGVGG KCENFEGGY 
      NCVCDDKSK CGHSCLLDN PCDSQPCVH GDCTPACTD 
      KADYICKCR EEYTGKNCS DYKVAASQM DHINILYII 
      IPIVLILSV GIAIGLAVL VNVARSKRA TRGTYSPSA 
      QEFCNPRVE LDHVLKPPP EERLI
    CDS sequence
    • >TC004424 RA
      atgggtttc ttgagttct tggaggttg ggaccaatt 
      tctgttttc ttggcaatt tgctgcatt tgtagcata 
      aattgccaa aacttggga ccatcaaat actccagaa 
      gcttatttc aacggctca gcttatcta cgtttacaa 
      acgactatt tctccaata aaacaaaca ggtctcagc 
      tttcggaca tgcacaggt ggcggccta ttttcccaa 
      caacaaaac gacgatttc ctcgaagtc tctgttaat 
      tcggagggc gtgatattt ttcgcaaaa acagccggc 
      aaacaattc aataacaaa attttcggt aatttcatc 
      aacaataaa tggcacgtt gtgttcctt tattacgag 
      caagggaac ttaactctg agagtcgat aacgaaaaa 
      cagttggtg gcaaattcg agttaccaa accgaactc 
      atcacaaac cccggcttt tacaacgag ggggcttca 
      gtcctcctc gtgggcaaa caattcaac ggttgtctc 
      ctcgaaggc ccctctctt gtcttcaac cattcgatt 
      atttacaac cacaatgtt aaattcgag ccttgcccc 
      attcctgaa gactcttgt attctccaa gtgatcgat 
      tactgcgtc actgagcct tgtatgcgt cgtggcacg 
      tgccacaac agccccaaa ggttacaat tgcacgtgc 
      cagccacga tacacggga aaaaattgc gagatggat 
      ttggggaat ccctgcgaa agaaatcca gcgatttgc 
      aagaacggg gccacgtgt aatgcggac aacacgggg 
      gtttacacg tgcacgtgt cccccaaat ttcacaggc 
      aaacactgt gaacaacta atcgttgtg aatcaacag 
      tgtcaggac aatccgtgc caaaatggg gccacgtgt 
      gtctcgaat ggaaatatg gagtgtttg tgtttaccg 
      ggtttcgat gggcctaag tgcgagttt aatatcgac 
      gattgtaag ggaaatccg tgcaagaat ggagggatt 
      tgcagggat ggactggat aatttcacc tgtgattgc 
      agtaggact ggatataca gggcgtttt tgtcaaatt 
      aacatcaat gaatgtgag acgagtcct tgtttgaat 
      catgggact tgtttcgac acgtatgga ggatttttg 
      tgccagtgt ccgccggga tatgggggc gcatattgt 
      caaaatact ttacacgca tgttcatca caacaatgt 
      ctcaacgag ggccaatgc attaacacc cctgacggt 
      tttaaatgc atttgccca gatgggttt gctggtgaa 
      cgttgcgaa gctggcgaa agacaaatt tcctgtgat 
      ggaacgaaa tgtccacca tatgccgac tgtgtaaaa 
      gccggcaac aattttgga tgtatttgc aaaccagaa 
      tatccagga aattatccg aattgttcc attcccaat 
      atttgtgct aataatcca tgcaaaaat caaggcatt 
      tgcacgtct tggaatggt tatttcaat tgttcgtgc 
      tcaccgggc ttcacaggt caactttgc gaaatacct 
      gtcgattcg caaccgaat tgtaactca aatccttgc 
      cagaatggg ggctcttgt ttcgataaa cccaccggt 
      ggtttttac tgcaattgc acggaccag tggatgggc 
      acttattgc aacgagtcg tatgatgtt tgtaaattg 
      gagccttgc cagaataac gctacttgt atttcttcg 
      caaaataag cgcgatttc gtctgcgaa tgtttgccg 
      ggctttgaa ggacaacat tgcgagagg aacatcgat 
      gattgtgtg ggggttacg tgcccctat ggtcaagtg 
      tgcttcgat ttggttaat gatcacgag tgtaggtgt 
      cctctgggt tataaaggg gagaattgt accattgac 
      gctgaccca tgtgccaaa aaaccgtgt atgaatggg 
      gccacatgt cagatgaac cataacgag aatgggttt 
      gtttgtaat tgtttggag ggtttttcg ggggagaga 
      tgcgaaacg gatattgat gagtgtaaa aatcagccg 
      ggaatttgc aatgagggg atctgccag aatgagctt 
      ggaggattt cagtgttat tgcaggccg gggtataca 
      ggggaacga tgcgatttg gattttgat gagtgttta 
      tcgatgccc tgtcggcat caagccact tgtcttaat 
      aaagttaat aattacgag tgcatttgt ccccctggg 
      tatgaaggc aaagactgc tcaattaat ataaatgag 
      tgcgagcca atgccgtgc atgaatggc tccacgtgc 
      attgatggg atcaataaa tttacgtgt aattgtcag 
      ccggggctt acggggaag atttgcgaa attaatatc 
      gacgattgt gagtcatct ccatgtctg aacggcgcc 
      gaatgcatc gacggcctc aacagctac acctgcaac 
      tgcaccgac accggctac accggcacc cactgcgag 
      acaaacatc aacgattgc ataggcgac ccttgcgaa 
      aacggcgct tcctgcgag gacaaagtc aaggactac 
      gattgccac tgctatgcc ggttattct ggcaaaaac 
      tgcgaaatc gacatcaac gaatgcgac agcaatccg 
      tgcaaacac aacggcaca tgttacgaa cactcgaac 
      caaaccctc taccaactc tctgaccaa accctcccc 
      gaaattttc tcggcggaa tttagctac gacaaagcc 
      gccggttac gaatgcctg tgcgttgac ggcgtcaca 
      ggcgaaaat tgcgaaatc gacatcaac gaatgcgaa 
      agcaatccg tgctttgaa ggcacttgt gtggataaa 
      atcgggggg tttgcctgc acgtgtgat gagggtttc 
      gagggcgag aggtgcgat atagacatc aatgaatgc 
      gaacggttc aaaccgtgc gtttacggg aaatgttgg 
      gataggaga gcgtcgtat tattgcgaa tgtgacgcg 
      aattacggc gggaaaaac tgttcggtt ggattaaca 
      ggctgtgat accaacccg tgtttgaac tccgggacg 
      tgcaaaccg tatttaatc gacgaaaac atccacagg 
      ttcaattgt tcgtgccct catggctat tacggacaa 
      gtttgcgaa aaaatcacc acgatgtcg ctatcgggg 
      aagtcccta attattgta aacacgtca cgggaggaa 
      ggctatgac atccagttc cgtttcaag acaacactg 
      ggcgatgga ttattggct ttgggcaaa ggcttgact 
      tattacatt cttgaatta tcccgaggg cgcctaaac 
      ttgcactca agcctgttg aataagtgg gagggtgtt 
      ttcatcgga tcgaactta aacgacagt caatggcaa 
      cgcgttttc gttgcgatt aactcaact catcttgtt 
      ttatcagcg aacgacgaa caaacaatt tatccgatc 
      agttttaac gaaatatca aatggcacc acctatacc 
      agtttcccc ttgacatac attgggggg atcccaagc 
      aatttgaaa aaactcacc catggtaaa acctacttg 
      gttggttgt accgaagac gtgctcatc aacggcgaa 
      tggattttg ccaccaagc tcaagccca cacacaaat 
      cccgcgttc acttttcaa gaagtggag ccaagttgt 
      aagagacag ccgcagtgt aatccgaac ccgtgtcat 
      tcagggggg cactgtacc gatctctgg gtcaatttt 
      aagtgtact tgtggacgg ccctacttg ggggacact 
      tgccaatac aattataca gctgcgact tttggttat 
      gaaaatata accgattct ttggttacg gtttacgtg 
      gacagtgct gcaagacgg gctgttcgt accatagtc 
      gatattagc atgtttata aggacgagg caaccaaaa 
      ggacagatt ttttatcta gggtccttg ccaaggact 
      atgaatccg tcggaggag acgtacatt gcagcacag 
      ttggaaggg ggcgaattg ttggtcagg atacagttt 
      aatggtact ccagaggct tatactgta ggcggtgtg 
      aagttggat aatggatac gaccattta attgaggtc 
      atcagaaac gtcacttta gtgcaagta aaactaaat 
      ggaactgaa tatttccgc aagacaatt tcagcaacg 
      ggtaccctc gatgctcaa gtcctcttt ctcggggga 
      cccccacaa acccgaccg gtgcgccaa gccaacgac 
      aacttagtc aaaatcgat acaatttcc ccaacttct 
      tcagccgta atagcaact cctttaagc aacgtccac 
      ttcaaggga atcatccaa gacgttcaa ataagcaac 
      ggatcgtcg gtcatgatc gtcgaattc ttccctctg 
      aacgtgaaa gacttagac atcccgaaa tcgttcggt 
      gaagttcga tttgacgaa acttcggtc cttgaaggc 
      gtccgatct gataactcc tgccgaata aatccgtgt 
      ttacacaac ggtgtgtgc gaaaacacg tggaatgat 
      tacagatgt atctgtacg cggggtttt aaggggaaa 
      gattgctcg gatttggag ttttgtgaa attgaaagg 
      tgtcccaaa gaatcagtt tgcaaaaat ctcgaagac 
      ggatctgaa tgtgtcgct aatgcgact tttgatggt 
      aaaacacca ccacttaaa taccggcta gtgacttgg 
      cccaattcg accaaaatc gtgtcttat gatacttta 
      gaagtgacc tatcggacg aggtcatgg gggaccatt 
      tttttcgcg aaacatgaa aacgacttt tttgccatt 
      tttatttat cataatgag gttgtggtg gaatggtct 
      attaacaaa atgggggat agtaaacgg ttcaggaag 
      gattatttc gaggggcag tggttgacc atctacttc 
      gaatacaaa aattcaatt ctgaaagga ggtttcaaa 
      gacatggtc atggacgaa gcgcctaat atgaaagtt 
      gccaacttt gatattgag ggttttaca cggatttta 
      acttttggt gatatttac gtcgcggga agcgatgaa 
      gtcacactt gattatcaa aatattatc aatagttca 
      aatgataat atgactggg tatatcccc tacagtgat 
      accaccaca acaccatcg accacaagt aattcagtt 
      gaagaaaat gacttcttt tctgatgtt tcgttgttc 
      aaagtggac caaaacaag tcaactgac ttttttaag 
      ggatgtatt aacgagatt aggatcggc ggtattttg 
      ctgccgttt ttcccatcg aatgaactt ttccaaaaa 
      tatcactat ttcgagctt tactccgaa aaccgtccc 
      caaattggt tgcattctc tgccacccg atcgactgt 
      tacaatcaa ggagtgtgt gccaaccct ctggaattt 
      tacaaatgt aactgtccg gccggtttt gcagccgac 
      gattgttcg atagacatc aatgaatgt gagaaaaac 
      gagtgtcag aacaatgcc acttgtata gatcttgtc 
      gctgaatac gagtgcaag tgcgaacca ggatttgag 
      ggcgaacat tgtgaaacc gacatcgat gaatgtgca 
      agtaacccg tgccgccat gggggcacc tgtaacgac 
      gccatcgga acattcaaa tgcgaatgc cccgaaggc 
      ttcgccggc aaacaatgc gaagcccct atactcata 
      acttgcgac aataaacca tgcaaagag ggcgccact 
      tgcaaaacg ggtccaaac gaaatcacc ggtaacaac 
      ttcacttgt atttgcacc ccaggcatg gagggcccc 
      ctttgtgac acccccttc tgttaccaa caaccttgc 
      ataaacaac ggtacgtgt gtcactgag accgaagta 
      ccgttctgt aattgcaca gttcggggt ttcgagggc 
      aaattctgc gaaataaac atcgacgat tgtgatatt 
      ccgactgga aatccgtgc caacacggc ggcatttgc 
      aacgaccaa gtaaacaaa tacgaatgc gattgtaat 
      gggacaggc tggggtggc ctactatgc gaaaacgac 
      atcaatgag tgtgtacaa atgcccgag ccgtgtggt 
      gttggcgga aaatgcgaa aacttcgaa gggggctat 
      aattgcgtc tgcgatgat aagtcaaag tgtgggcat 
      tcgtgtctt ttggacaac ccctgtgat tcccagcct 
      tgtgttcat ggggattgt actcctgca tgcaccgac 
      aaggccgac tatatttgc aaatgtagg gaggagtac 
      accgggaaa aattgcagt gattataaa gtagcagcg 
      agtcaaatg gaccatatt aacattttg tatatcatt 
      atcccgatt gttttgatt ttgagtgta ggtatcgcg 
      attgggttg gcagtactt gttaatgtg gctcggagt 
      aaaagggca acgagggga acgtatagt ccaagtgcc 
      caggaattt tgtaatccc agagttgag ttggaccat 
      gttttgaaa cctccaccc gaggaaagg cttatttaa
    mRNA sequence
    • >TC004424 RA
      aattgtcac tagaaaaag tgttgtgtg ggtgctggg 
      tgttgttgt cttgtgaac ctgtcgttt attttgtgt 
      ttatttact ggttttaaa taataaaat tatgttttt 
      ttcaacata aacacacct ccccgttgt tgcttcatg 
      gtgcatatg attttaatg aatgtaacg aaaaaaccg 
      caatccagt tgtctcaaa gatgggttt cttgagttc 
      ttggaggtt gggaccaat ttctgtttt cttggcaat 
      ttgctgcat ttgtagcat aaattgcca aaacttggg 
      accatcaaa tactccaga agcttattt caacggctc 
      agcttatct acgtttaca aacgactat ttctccaat 
      aaaacaaac aggtctcag ctttcggac atgcacagg 
      tggcggcct attttccca acaacaaaa cgacgattt 
      cctcgaagt ctctgttaa ttcggaggg cgtgatatt 
      tttcgcaaa aacagccgg caaacaatt caataacaa 
      aattttcgg taatttcat caacaataa atggcacgt 
      tgtgttcct ttattacga gcaagggaa cttaactct 
      gagagtcga taacgaaaa acagttggt ggcaaattc 
      gagttacca aaccgaact catcacaaa ccccggctt 
      ttacaacga gggggcttc agtcctcct cgtgggcaa 
      acaattcaa cggttgtct cctcgaagg cccctctct 
      tgtcttcaa ccattcgat tatttacaa ccacaatgt 
      taaattcga gccttgccc cattcctga agactcttg 
      tattctcca agtgatcga ttactgcgt cactgagcc 
      ttgtatgcg tcgtggcac gtgccacaa cagccccaa 
      aggttacaa ttgcacgtg ccagccacg atacacggg 
      aaaaaattg cgagatgga tttggggaa tccctgcga 
      aagaaatcc agcgatttg caagaacgg ggccacgtg 
      taatgcgga caacacggg ggtttacac gtgcacgtg 
      tcccccaaa tttcacagg caaacactg tgaacaact 
      aatcgttgt gaatcaaca gtgtcagga caatccgtg 
      ccaaaatgg ggccacgtg tgtctcgaa tggaaatat 
      ggagtgttt gtgtttacc gggtttcga tgggcctaa 
      gtgcgagtt taatatcga cgattgtaa gggaaatcc 
      gtgcaagaa tggagggat ttgcaggga tggactgga 
      taatttcac ctgtgattg cagtaggac tggatatac 
      agggcgttt ttgtcaaat taacatcaa tgaatgtga 
      gacgagtcc ttgtttgaa tcatgggac ttgtttcga 
      cacgtatgg aggattttt gtgccagtg tccgccggg 
      atatggggg cgcatattg tcaaaatac tttacacgc 
      atgttcatc acaacaatg tctcaacga gggccaatg 
      cattaacac ccctgacgg ttttaaatg catttgccc 
      agatgggtt tgctggtga acgttgcga agctggcga 
      aagacaaat ttcctgtga tggaacgaa atgtccacc 
      atatgccga ctgtgtaaa agccggcaa caattttgg 
      atgtatttg caaaccaga atatccagg aaattatcc 
      gaattgttc cattcccaa tatttgtgc taataatcc 
      atgcaaaaa tcaaggcat ttgcacgtc ttggaatgg 
      ttatttcaa ttgttcgtg ctcaccggg cttcacagg 
      tcaactttg cgaaatacc tgtcgattc gcaaccgaa 
      ttgtaactc aaatccttg ccagaatgg gggctcttg 
      tttcgataa acccaccgg tggttttta ctgcaattg 
      cacggacca gtggatggg cacttattg caacgagtc 
      gtatgatgt ttgtaaatt ggagccttg ccagaataa 
      cgctacttg tatttcttc gcaaaataa gcgcgattt 
      cgtctgcga atgtttgcc gggctttga aggacaaca 
      ttgcgagag gaacatcga tgattgtgt gggggttac 
      gtgccccta tggtcaagt gtgcttcga tttggttaa 
      tgatcacga gtgtaggtg tcctctggg ttataaagg 
      ggagaattg taccattga cgctgaccc atgtgccaa 
      aaaaccgtg tatgaatgg ggccacatg tcagatgaa 
      ccataacga gaatgggtt tgtttgtaa ttgtttgga 
      gggtttttc gggggagag atgcgaaac ggatattga 
      tgagtgtaa aaatcagcc gggaatttg caatgaggg 
      gatctgcca gaatgagct tggaggatt tcagtgtta 
      ttgcaggcc ggggtatac aggggaacg atgcgattt 
      ggattttga tgagtgttt atcgatgcc ctgtcggca 
      tcaagccac ttgtcttaa taaagttaa taattacga 
      gtgcatttg tccccctgg gtatgaagg caaagactg 
      ctcaattaa tataaatga gtgcgagcc aatgccgtg 
      catgaatgg ctccacgtg cattgatgg gatcaataa 
      atttacgtg taattgtca gccggggct tacggggaa 
      gatttgcga aattaatat cgacgattg tgagtcatc 
      tccatgtct gaacggcgc cgaatgcat cgacggcct 
      caacagcta cacctgcaa ctgcaccga caccggcta 
      caccggcac ccactgcga gacaaacat caacgattg 
      cataggcga cccttgcga aaacggcgc ttcctgcga 
      ggacaaagt caaggacta cgattgcca ctgctatgc 
      cggttattc tggcaaaaa ctgcgaaat cgacatcaa 
      cgaatgcga cagcaatcc gtgcaaaca caacggcac 
      atgttacga acactcgaa ccaaaccct ctaccaact 
      ctctgacca aaccctccc cgaaatttt ctcggcgga 
      atttagcta cgacaaagc cgccggtta cgaatgcct 
      gtgcgttga cggcgtcac aggcgaaaa ttgcgaaat 
      cgacatcaa cgaatgcga aagcaatcc gtgctttga 
      aggcacttg tgtggataa aatcggggg gtttgcctg 
      cacgtgtga tgagggttt cgagggcga gaggtgcga 
      tatagacat caatgaatg cgaacggtt caaaccgtg 
      cgtttacgg gaaatgttg ggataggag agcgtcgta 
      ttattgcga atgtgacgc gaattacgg cgggaaaaa 
      ctgttcggt tggattaac aggctgtga taccaaccc 
      gtgtttgaa ctccgggac gtgcaaacc gtatttaat 
      cgacgaaaa catccacag gttcaattg ttcgtgccc 
      tcatggcta ttacggaca agtttgcga aaaaatcac 
      cacgatgtc gctatcggg gaagtccct aattattgt 
      aaacacgtc acgggagga aggctatga catccagtt 
      ccgtttcaa gacaacact gggcgatgg attattggc 
      tttgggcaa aggcttgac ttattacat tcttgaatt 
      atcccgagg gcgcctaaa cttgcactc aagcctgtt 
      gaataagtg ggagggtgt tttcatcgg atcgaactt 
      aaacgacag tcaatggca acgcgtttt cgttgcgat 
      taactcaac tcatcttgt tttatcagc gaacgacga 
      acaaacaat ttatccgat cagttttaa cgaaatatc 
      aaatggcac cacctatac cagtttccc cttgacata 
      cattggggg gatcccaag caatttgaa aaaactcac 
      ccatggtaa aacctactt ggttggttg taccgaaga 
      cgtgctcat caacggcga atggatttt gccaccaag 
      ctcaagccc acacacaaa tcccgcgtt cacttttca 
      agaagtgga gccaagttg taagagaca gccgcagtg 
      taatccgaa cccgtgtca ttcaggggg gcactgtac 
      cgatctctg ggtcaattt taagtgtac ttgtggacg 
      gccctactt gggggacac ttgccaata caattatac 
      agctgcgac ttttggtta tgaaaatat aaccgattc 
      tttggttac ggtttacgt ggacagtgc tgcaagacg 
      ggctgttcg taccatagt cgatattag catgtttat 
      aaggacgag gcaaccaaa aggacagat tttttatct 
      agggtcctt gccaaggac tatgaatcc gtcggagga 
      gacgtacat tgcagcaca gttggaagg gggcgaatt 
      gttggtcag gatacagtt taatggtac tccagaggc 
      ttatactgt aggcggtgt gaagttgga taatggata 
      cgaccattt aattgaggt catcagaaa cgtcacttt 
      agtgcaagt aaaactaaa tggaactga atatttccg 
      caagacaat ttcagcaac gggtaccct cgatgctca 
      agtcctctt tctcggggg acccccaca aacccgacc 
      ggtgcgcca agccaacga caacttagt caaaatcga 
      tacaatttc cccaacttc ttcagccgt aatagcaac 
      tcctttaag caacgtcca cttcaaggg aatcatcca 
      agacgttca aataagcaa cggatcgtc ggtcatgat 
      cgtcgaatt cttccctct gaacgtgaa agacttaga 
      catcccgaa atcgttcgg tgaagttcg atttgacga 
      aacttcggt ccttgaagg cgtccgatc tgataactc 
      ctgccgaat aaatccgtg tttacacaa cggtgtgtg 
      cgaaaacac gtggaatga ttacagatg tatctgtac 
      gcggggttt taaggggaa agattgctc ggatttgga 
      gttttgtga aattgaaag gtgtcccaa agaatcagt 
      ttgcaaaaa tctcgaaga cggatctga atgtgtcgc 
      taatgcgac ttttgatgg taaaacacc accacttaa 
      ataccggct agtgacttg gcccaattc gaccaaaat 
      cgtgtctta tgatacttt agaagtgac ctatcggac 
      gaggtcatg ggggaccat ttttttcgc gaaacatga 
      aaacgactt ttttgccat ttttattta tcataatga 
      ggttgtggt ggaatggtc tattaacaa aatggggga 
      tagtaaacg gttcaggaa ggattattt cgaggggca 
      gtggttgac catctactt cgaatacaa aaattcaat 
      tctgaaagg aggtttcaa agacatggt catggacga 
      agcgcctaa tatgaaagt tgccaactt tgatattga 
      gggttttac acggatttt aacttttgg tgatattta 
      cgtcgcggg aagcgatga agtcacact tgattatca 
      aaatattat caatagttc aaatgataa tatgactgg 
      gtatatccc ctacagtga taccaccac aacaccatc 
      gaccacaag taattcagt tgaagaaaa tgacttctt 
      ttctgatgt ttcgttgtt caaagtgga ccaaaacaa 
      gtcaactga cttttttaa gggatgtat taacgagat 
      taggatcgg cggtatttt gctgccgtt tttcccatc 
      gaatgaact tttccaaaa atatcacta tttcgagct 
      ttactccga aaaccgtcc ccaaattgg ttgcattct 
      ctgccaccc gatcgactg ttacaatca aggagtgtg 
      tgccaaccc tctggaatt ttacaaatg taactgtcc 
      ggccggttt tgcagccga cgattgttc gatagacat 
      caatgaatg tgagaaaaa cgagtgtca gaacaatgc 
      cacttgtat agatcttgt cgctgaata cgagtgcaa 
      gtgcgaacc aggatttga gggcgaaca ttgtgaaac 
      cgacatcga tgaatgtgc aagtaaccc gtgccgcca 
      tgggggcac ctgtaacga cgccatcgg aacattcaa 
      atgcgaatg ccccgaagg cttcgccgg caaacaatg 
      cgaagcccc tatactcat aacttgcga caataaacc 
      atgcaaaga gggcgccac ttgcaaaac gggtccaaa 
      cgaaatcac cggtaacaa cttcacttg tatttgcac 
      cccaggcat ggagggccc cctttgtga caccccctt 
      ctgttacca acaaccttg cataaacaa cggtacgtg 
      tgtcactga gaccgaagt accgttctg taattgcac 
      agttcgggg tttcgaggg caaattctg cgaaataaa 
      catcgacga ttgtgatat tccgactgg aaatccgtg 
      ccaacacgg cggcatttg caacgacca agtaaacaa 
      atacgaatg cgattgtaa tgggacagg ctggggtgg 
      cctactatg cgaaaacga catcaatga gtgtgtaca 
      aatgcccga gccgtgtgg tgttggcgg aaaatgcga 
      aaacttcga agggggcta taattgcgt ctgcgatga 
      taagtcaaa gtgtgggca ttcgtgtct tttggacaa 
      cccctgtga ttcccagcc ttgtgttca tggggattg 
      tactcctgc atgcaccga caaggccga ctatatttg 
      caaatgtag ggaggagta caccgggaa aaattgcag 
      tgattataa agtagcagc gagtcaaat ggaccatat 
      taacatttt gtatatcat tatcccgat tgttttgat 
      tttgagtgt aggtatcgc gattgggtt ggcagtact 
      tgttaatgt ggctcggag taaaagggc aacgagggg 
      aacgtatag tccaagtgc ccaggaatt ttgtaatcc 
      cagagttga gttggacca tgttttgaa acctccacc 
      cgaggaaag gcttattta attgttttt tgttaattt 
      tgacggcat tcgaatcag taggtttag tatttattg 
      tagatactc atttgttcg gtttttacg caaagtaat 
      gtaacatag tacttaatc tttttatta atgatagta 
      ttatttaaa acagtgcgc tgttaacgg caataatca 
      atttcaagc cgtccattg tagtattgt gataatggt 
      aggacaaaa gtgtaatta ttaagaaaa atgtagacc 
      aaagtgtgt atatatttt tttatctat atagatttt 
      ctacgtttt atttggtgt aatttatga agtgatgca 
      agtcggatt tgtacttaa tgctctgta acgtttaaa 
      taaaattcc aacactttt
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