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Gene information
General information
TC004435 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4952046..4954497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004435
    iBeetle screen iB_09597

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004435 RA
      MHFITLVFL VLTAASAGR AFNVHYLHQ GEKFSAGVQ 
      RMPPEFHIF CYAGKPKYL IHIWQSVML QLDHKSGDY 
      TQYDGPSPD AVMEEFNAN RYSWNLNLF TNKQKQMHL 
      DPFNESCIG IDSRESYTV TLNVIRIDY WKVLLLLGG 
      ISLFLTANR LSRNTLFYY ICGISLGIC TSFLILIYL 
      ISRLFPRKP IMYGVAAFG WTVGIYLLQ LLWDNVRVI 
      LLNYKSYVV WYTLITGFI SFIICYRWG PIENERSRN 
      LIRWALQGA GLIAIFFST QFQEAAMGQ IVALLLFHN 
      VPSTWLLKP KTYWKKRFP PKIKPLTND EYYEQGVRE 
      TTKALEDLR KYCSSPECK QWQVAMKLK DVKRFASFM 
      EGNSHLSDE EILEYETSI HTTDLTDDE SEYTEDEQC 
      F
    CDS sequence
    • >TC004435 RA
      atgcacttt attacttta gtctttttg gttctcacg 
      gccgcttcc gcgggtcgg gccttcaat gttcattac 
      ttgcatcag ggagagaag ttctcagct ggggtgcaa 
      aggatgccc ccagagttt catattttt tgctacgcg 
      ggaaagccc aagtatttg atacacatt tggcaaagt 
      gttatgctc cagttggat cataaatcc ggtgattat 
      actcaatat gacggtccg tcccctgat gccgttatg 
      gaagaattc aatgctaat aggtacagt tggaattta 
      aatttattt acaaataag cagaagcag atgcatttg 
      gacccattc aatgagagc tgtattgga atagacagc 
      agggagagc tacacagtg accctaaat gtaatcaga 
      atagattac tggaaagtg cttcttcta ctagggggc 
      atttcactc tttcttaca gcaaacaga ctgagcaga 
      aacacctta ttttattac atttgtggc atttcactc 
      ggaatttgt acttcgttc ctgattctc atttattta 
      attagcaga cttttcccg aggaaacca atcatgtac 
      ggagtggct gcgtttgga tggacggtc ggaatctac 
      cttttgcaa cttttgtgg gacaacgtc cgtgtcatt 
      ttactaaat tacaaatcc tacgttgtg tggtacact 
      ctaataact ggttttata agtttcata atttgctac 
      agatggggc cccatagag aacgagaga agccgcaat 
      ttgatccga tgggccctg caaggggct ggtcttatt 
      gcaattttt ttcagcacc caattccag gaggcggca 
      atgggtcaa atcgttgcc cttttactc ttccacaac 
      gtgccatca acgtggctt ttaaaaccg aaaacatac 
      tggaaaaaa cgatttccg ccaaaaatt aaaccacta 
      actaacgac gagtattac gagcaggga gttagggaa 
      acaacgaaa gcgttggag gatttgagg aaatattgc 
      tcaagtcct gagtgtaaa cagtggcaa gttgctatg 
      aaattgaaa gatgtcaaa agatttgcg tcgtttatg 
      gaaggcaat tcgcatttg agtgatgag gaaattcta 
      gagtacgaa acgtcgatc cacacgacg gatttaacg 
      gacgatgag agtgaatat acggaggat gagcagtgt 
      ttttaa
    mRNA sequence
    • >TC004435 RA
      ctaacttct tagagactg tcaaacaaa aaatgtaac 
      ggaaactca aaatacaaa attatttca ttgtagttc 
      acgaatttt caaatttat acgtaaaat gcactttat 
      tactttagt ctttttggt tctcacggc cgcttccgc 
      gggtcgggc cttcaatgt tcattactt gcatcaggg 
      agagaagtt ctcagctgg ggtgcaaag gatgccccc 
      agagtttca tattttttg ctacgcggg aaagcccaa 
      gtatttgat acacatttg gcaaagtgt tatgctcca 
      gttggatca taaatccgg tgattatac tcaatatga 
      cggtccgtc ccctgatgc cgttatgga agaattcaa 
      tgctaatag gtacagttg gaatttaaa tttatttac 
      aaataagca gaagcagat gcatttgga cccattcaa 
      tgagagctg tattggaat agacagcag ggagagcta 
      cacagtgac cctaaatgt aatcagaat agattactg 
      gaaagtgct tcttctact agggggcat ttcactctt 
      tcttacagc aaacagact gagcagaaa caccttatt 
      ttattacat ttgtggcat ttcactcgg aatttgtac 
      ttcgttcct gattctcat ttatttaat tagcagact 
      tttcccgag gaaaccaat catgtacgg agtggctgc 
      gtttggatg gacggtcgg aatctacct tttgcaact 
      tttgtggga caacgtccg tgtcatttt actaaatta 
      caaatccta cgttgtgtg gtacactct aataactgg 
      ttttataag tttcataat ttgctacag atggggccc 
      catagagaa cgagagaag ccgcaattt gatccgatg 
      ggccctgca aggggctgg tcttattgc aattttttt 
      cagcaccca attccagga ggcggcaat gggtcaaat 
      cgttgccct tttactctt ccacaacgt gccatcaac 
      gtggctttt aaaaccgaa aacatactg gaaaaaacg 
      atttccgcc aaaaattaa accactaac taacgacga 
      gtattacga gcagggagt tagggaaac aacgaaagc 
      gttggagga tttgaggaa atattgctc aagtcctga 
      gtgtaaaca gtggcaagt tgctatgaa attgaaaga 
      tgtcaaaag atttgcgtc gtttatgga aggcaattc 
      gcatttgag tgatgagga aattctaga gtacgaaac 
      gtcgatcca cacgacgga tttaacgga cgatgagag 
      tgaatatac ggaggatga gcagtgttt ttaagactg 
      acaatttcg ttaattatt tatttactt atttgcatt 
      aatattgta aaaactgta aaatacaat taattttta 
      cttaaagct ggtacatcc aaggccaca aaaatcaaa 
      taaaaaaat cgtaattag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY