Status
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Gene information
General information
TC032018
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004465
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4627227..4635508
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032018
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09594

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032018 RA
      MTFVWKVVF VFFVSSVTY SEEPKDGYY VDDLNEASF 
      TTGSEPLVC PSTNVISTR YKCNVNGEW VDCTRKHCC 
      KDYTFVAGR CIPRSQDPC SMGLCEQVC TVYLQRVIC 
      TCFDGYKFN PENQKRGIK PVCVDVDEC LDNNGDCEH 
      ECINESGSY RCACRAGFT LRADNRTCE PLNNLEGTA 
      EQAGHSNRC FANCDTVLR LHDKLKSLQ EKVSALSTA 
      IRLSSFASG PPGPPGPPG PVGPPGPRG FPGAESEAL 
      SAHTTQDYT YSVLDAFVP LPGDENAQC RCKRGPQGE 
      AGPRGPQGP KGEQGEKGP RGPKGERGS FDFLLLLLA 
      DVKHDIVHL QNRVYRNGE APPKFDIDA ALHKKRIKD 
      KHTFLKQKR LLEAYTSIP TVVTHTTTE TTDSTQVEE 
      FRDGHVPDD YLDYYGSGS ESEDYMN
    CDS sequence
    • >TC032018 RA
      atgacgttc gtttggaag gtggtcttt gtgtttttc 
      gtgtcgagt gttacgtat agcgaagag ccgaaagat 
      ggctactat gtagatgat ctcaatgag gcttccttt 
      acaacaggg tccgagccg ctcgtttgc ccatccacc 
      aacgtcatt tcaacaagg tacaaatgt aacgtcaat 
      ggggagtgg gtggattgc acgcggaaa cactgctgc 
      aaggactac acttttgta gcaggaagg tgcattcct 
      agatcgcag gatccgtgt tctatgggc ttgtgcgag 
      caagtttgt actgtttat ttacaacgc gtcatttgc 
      acctgtttt gacggctac aaattcaat ccggaaaac 
      caaaaacga ggaattaaa cctgtctgc gtcgatgta 
      gacgagtgt ttagataat aacggagac tgtgaacac 
      gaatgcata aacgaatct ggaagctac cgttgcgca 
      tgtagagca ggatttaca ttgcgagcg gacaataga 
      acatgcgaa cctttaaat aatttagaa ggaacagca 
      gaacaagcc ggacacagt aatcgctgt ttcgccaac 
      tgcgacacc gttctgcga cttcatgac aagctaaaa 
      tcgttgcag gaaaaggta tccgctttg agtacggca 
      atcagattg tcgagtttc gcttcgggg ccgccaggg 
      cccccgggg ccccccggc cccgtgggc ccgccgggg 
      ccgagagga ttcccaggg gcggagagc gaagcactg 
      tctgctcac accacacaa gattacact tattcggtt 
      ttggatgcg tttgtgcct ttgccaggg gatgagaat 
      gcccaatgt cggtgcaaa agagggcca caaggcgag 
      gcggggccg agagggcct caaggaccc aaaggtgag 
      caaggagaa aaggggccg agagggcca aagggcgaa 
      aggggctcg tttgacttc ctgctgctt ttactagct 
      gatgtgaaa catgatatc gttcactta cagaatcgg 
      gtttatagg aacggggaa gcaccaccg aaattcgac 
      attgacgcc gccctccac aaaaagcga atcaaagac 
      aaacacact tttctcaag caaaaacga ttgttggaa 
      gcttacact tcaattcct actgttgtt acacacact 
      acaacggaa acgacagat tcgacacaa gtggaagaa 
      tttcgagat ggacatgtg cctgatgac tatttagat 
      tattacgga tcaggaagc gaatcggaa gattatatg 
      aattaa
    mRNA sequence
    • >TC032018 RA
      aaacactcc tgaaaaatt tcgcaccgc cactgtttt 
      agtcctgat gatgataca gatcggttt tatacaagg 
      ctgtattaa ttggaccgt ttggtgtgg aaagaagta 
      ttttgtaaa gaaatgagg ataatcgcg tgttagctg 
      catcgtaat ccgtccgca ctcccttaa ttgctaaat 
      tacaaccgc actcttcat tttcgaaac aaagttgcg 
      taaagcaat ggttaattg gtttagtcg cgaaaaaat 
      cgtctgtta gtgaaacaa tttgaacat tttaatcaa 
      aagttgaaa tgattaaac tcaagaatt tgtaattaa 
      attaaatgt tgggcgaat tttttgtga gtgtaatta 
      tgacgttcg tttggaagg tggtctttg tgtttttcg 
      tgtcgagtg ttacgtata gcgaagagc cgaaagatg 
      gctactatg tagatgatc tcaatgagg cttccttta 
      caacagggt ccgagccgc tcgtttgcc catccacca 
      acgtcattt caacaaggt acaaatgta acgtcaatg 
      gggagtggg tggattgca cgcggaaac actgctgca 
      aggactaca cttttgtag caggaaggt gcattccta 
      gatcgcagg atccgtgtt ctatgggct tgtgcgagc 
      aagtttgta ctgtttatt tacaacgcg tcatttgca 
      cctgttttg acggctaca aattcaatc cggaaaacc 
      aaaaacgag gaattaaac ctgtctgcg tcgatgtag 
      acgagtgtt tagataata acggagact gtgaacacg 
      aatgcataa acgaatctg gaagctacc gttgcgcat 
      gtagagcag gatttacat tgcgagcgg acaatagaa 
      catgcgaac ctttaaata atttagaag gaacagcag 
      aacaagccg gacacagta atcgctgtt tcgccaact 
      gcgacaccg ttctgcgac ttcatgaca agctaaaat 
      cgttgcagg aaaaggtat ccgctttga gtacggcaa 
      tcagattgt cgagtttcg cttcggggc cgccagggc 
      ccccggggc cccccggcc ccgtgggcc cgccggggc 
      cgagaggat tcccagggg cggagagcg aagcactgt 
      ctgctcaca ccacacaag attacactt attcggttt 
      tggatgcgt ttgtgcctt tgccagggg atgagaatg 
      cccaatgtc ggtgcaaaa gagggccac aaggcgagg 
      cggggccga gagggcctc aaggaccca aaggtgagc 
      aaggagaaa aggggccga gagggccaa agggcgaaa 
      ggggctcgt ttgacttcc tgctgcttt tactagctg 
      atgtgaaac atgatatcg ttcacttac agaatcggg 
      tttatagga acggggaag caccaccga aattcgaca 
      ttgacgccg ccctccaca aaaagcgaa tcaaagaca 
      aacacactt ttctcaagc aaaaacgat tgttggaag 
      cttacactt caattccta ctgttgtta cacacacta 
      caacggaaa cgacagatt cgacacaag tggaagaat 
      ttcgagatg gacatgtgc ctgatgact atttagatt 
      attacggat caggaagcg aatcggaag attatatga 
      attaaactc tgttttaat aaaagtatt ttattcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY