Status
Please wait ...
Gene information
General information
TC004467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4619971..4622488
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004467
    iBeetle screen iB_09593

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004467 RA
      MFGVVCPQC KKASNGAAN RLVRDSCGH EKCRVCLLL 
      DEEKCQQCK TENTEVKEQ EPPSVIKCE SHTGVITCN 
      GTAKKTPIC PIAQNGGVE KKQPTKTSS KNLPNRNLF 
      NDISVPNHV TVLKDPLQY KCNICNKKF TTKSHIKYH 
      TYCTGQRKP FQCNLCSRD FITKSHLDV HLLKHTGTK 
      PFTCNICQK SFSERSKLN RHSLLHSTV KPYMCSECG 
      KAFRSKESL KIHTLIHRG EKPYSCRVC NISFNNTSN 
      LNKHLITHS NEKSHMCDQ CGKRFKLKW ALTVHRRSH 
      LKDRPFECT SCSRAFVNN KDLQRHQLI HLETKAYNC 
      SICNTSFRR KDNLYRHVK NTHPGKKAE PVTTTVKQL 
      LVKPNKPVD NPNAINVIT ASPACKTKP VNNRTGTVI 
      NGPLKLAFK TSAFKNNYN IHRDCDDDL IPNVSKPYD 
      LAESVNICQ KILSPHSPP ARNIIRTST EPTSTEICQ 
      NILSPYSRP IHYETSFEH KKHAIIKNI KFKLPPNYT 
      NLKPTNINF NVDTDLANN KTYTELKTV QNAATSVIV 
      NSNLSQNSN MHWRRRTLQ NLCSRNLEN
    CDS sequence
    • >TC004467 RA
      atgtttggg gtggtttgc ccccaatgc aagaaagca 
      tcgaacggg gctgccaac cgactggta agagattcc 
      tgcggtcat gaaaagtgc cgtgtgtgt cttctcctc 
      gacgaggaa aaatgtcaa cagtgtaaa actgaaaat 
      actgaggtt aaagaacaa gagcctcca agtgttatc 
      aaatgtgag agccacact ggcgtgata acgtgtaat 
      ggcacggcg aaaaagaca ccaatttgc cccattgcc 
      cagaatgga ggagttgag aagaaacaa ccgactaaa 
      acatcatca aaaaattta cctaaccga aatttgttt 
      aatgatatt tctgtcccc aatcatgtt actgttctt 
      aaggatccc cttcaatat aaatgcaac atttgtaac 
      aagaaattt accacaaag tcgcatatt aagtaccac 
      acctactgt acaggacag aggaaacct tttcaatgc 
      aatttgtgc tccagagat tttattact aagtcacac 
      ctagacgtt catctttta aagcacact ggaaccaaa 
      ccgttcacc tgtaacatc tgccagaaa tctttcagc 
      gaaagaagc aaattaaat cgtcacagt cttttacat 
      tcaacagtc aaaccgtac atgtgttca gagtgtggt 
      aaagcattc cgtagcaag gaatcactc aaaatccat 
      accttgatc catcgcggc gaaaaacca tactcctgc 
      agggtgtgc aacatcagt ttcaacaat acctcaaac 
      ttaaacaaa catctcata acgcattca aatgaaaaa 
      tcgcacatg tgtgaccag tgtggcaaa agatttaaa 
      ttgaaatgg gctttgaca gttcaccga cgatctcat 
      ttaaaagac cgacctttt gaatgcacc agttgctca 
      agggctttt gttaataat aaagatttg caacgtcat 
      caacttatc cacttagaa acgaaagct tacaattgc 
      agcatttgc aacactagt tttcggcgc aaagataac 
      ttataccgg catgtgaaa aacacacat ccgggcaaa 
      aaagccgaa cctgttaca actaccgtc aaacaatta 
      ctcgtcaaa cctaacaaa cccgtggat aatcccaat 
      gcgattaat gtgataaca gcgtcgccg gcttgtaaa 
      accaaaccg gtgaataat cgcaccgga actgtgatt 
      aacggcccc ttgaagctt gcatttaag actagcgca 
      tttaaaaat aattacaat attcataga gattgtgac 
      gacgatctg ataccgaac gtatcaaaa ccgtacgat 
      ttggccgag tcggtgaat atttgtcag aagattttg 
      tcgccgcac tcgcctccg gctcgtaac ataattcgg 
      acatcgaca gagccgact tccaccgaa atctgtcag 
      aatatcctt tccccatat tccagacct attcattac 
      gagacttct ttcgaacat aaaaaacac gcaattatt 
      aaaaatatc aaattcaag ttgcctcca aattacacg 
      aatcttaaa ccgacgaat attaatttc aacgttgac 
      acggacttg gctaataat aagacttac acagaactg 
      aaaacggtg caaaatgcc gccaccagt gtcattgtt 
      aacagcaat ttgagccaa aatagtaac atgcactgg 
      aggagaagg acgttgcaa aatctttgt tcgaggaat 
      ttagagaat tga
    mRNA sequence
    • >TC004467 RA
      agtagttca aaattacgc cgccatttt tcttattgt 
      tattgtcaa aaagcattt ttattcacg tgtttgaag 
      ccctaaact acagttcta gtgtctcat tatcataat 
      aaacctaat cttattgtc ctgtagtga aaaatgtga 
      tcttcgagt gttttttat aatctaacc ttaattgaa 
      ttggcaatg tttggggtg gtttgcccc caatgcaag 
      aaagcatcg aacggggct gccaaccga ctggtaaga 
      gattcctgc ggtcatgaa aagtgccgt gtgtgtctt 
      ctcctcgac gaggaaaaa tgtcaacag tgtaaaact 
      gaaaatact gaggttaaa gaacaagag cctccaagt 
      gttatcaaa tgtgagagc cacactggc gtgataacg 
      tgtaatggc acggcgaaa aagacacca atttgcccc 
      attgcccag aatggagga gttgagaag aaacaaccg 
      actaaaaca tcatcaaaa aatttacct aaccgaaat 
      ttgtttaat gatatttct gtccccaat catgttact 
      gttcttaag gatcccctt caatataaa tgcaacatt 
      tgtaacaag aaatttacc acaaagtcg catattaag 
      taccacacc tactgtaca ggacagagg aaacctttt 
      caatgcaat ttgtgctcc agagatttt attactaag 
      tcacaccta gacgttcat cttttaaag cacactgga 
      accaaaccg ttcacctgt aacatctgc cagaaatct 
      ttcagcgaa agaagcaaa ttaaatcgt cacagtctt 
      ttacattca acagtcaaa ccgtacatg tgttcagag 
      tgtggtaaa gcattccgt agcaaggaa tcactcaaa 
      atccatacc ttgatccat cgcggcgaa aaaccatac 
      tcctgcagg gtgtgcaac atcagtttc aacaatacc 
      tcaaactta aacaaacat ctcataacg cattcaaat 
      gaaaaatcg cacatgtgt gaccagtgt ggcaaaaga 
      tttaaattg aaatgggct ttgacagtt caccgacga 
      tctcattta aaagaccga ccttttgaa tgcaccagt 
      tgctcaagg gcttttgtt aataataaa gatttgcaa 
      cgtcatcaa cttatccac ttagaaacg aaagcttac 
      aattgcagc atttgcaac actagtttt cggcgcaaa 
      gataactta taccggcat gtgaaaaac acacatccg 
      ggcaaaaaa gccgaacct gttacaact accgtcaaa 
      caattactc gtcaaacct aacaaaccc gtggataat 
      cccaatgcg attaatgtg ataacagcg tcgccggct 
      tgtaaaacc aaaccggtg aataatcgc accggaact 
      gtgattaac ggccccttg aagcttgca tttaagact 
      agcgcattt aaaaataat tacaatatt catagagat 
      tgtgacgac gatctgata ccgaacgta tcaaaaccg 
      tacgatttg gccgagtcg gtgaatatt tgtcagaag 
      attttgtcg ccgcactcg cctccggct cgtaacata 
      attcggaca tcgacagag ccgacttcc accgaaatc 
      tgtcagaat atcctttcc ccatattcc agacctatt 
      cattacgag acttctttc gaacataaa aaacacgca 
      attattaaa aatatcaaa ttcaagttg cctccaaat 
      tacacgaat cttaaaccg acgaatatt aatttcaac 
      gttgacacg gacttggct aataataag acttacaca 
      gaactgaaa acggtgcaa aatgccgcc accagtgtc 
      attgttaac agcaatttg agccaaaat agtaacatg 
      cactggagg agaaggacg ttgcaaaat ctttgttcg 
      aggaattta gagaattga agactgttg aaggctttt 
      cgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY