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Gene information
General information
TC004910 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4517468..4526323
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004910
    iBeetle screen iB_09592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax potentially partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen potentially circumference decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004910 RA
      MTIHTKIAI SKVYNYTHN TSLYKNFFF LVFIVLIVT 
      TFTVLPRLR INIGDESSN FKKITLVNI RNLPEFRLV 
      GDSASPRNP ECTHWDCFN IYKCGRTGH DRIAVYVYP 
      PKKYVDEQG VPATELMSK EYLALLQAV VNSKYYTAN 
      PHEACIFIP SIDTLNQER LRPNLTSKA LRSLPFWND 
      GENHLIFNM IAGNAPDFS PVVDLATGK ALIAGADFN 
      KYSFRTNFD VSIPLFSPI ARPGDINHR RRSWLVTSS 
      QLNLDVNYL GVLQKLSSR HNSLLILDA CHRHNYTKR 
      CRENSNEMV DYPAILHES TFCLVFRGE RIGQFALLE 
      AMAANCIPV VVMDGAVLP FSNVIDWKR AAVFIMENY 
      LHTLVDVLE KISPQRIKQ MQKTGRFLY DSYFSSIEK 
      IALTTLDII QDRVYPHWS KIYDDWNLR PEEKNTNPL 
      FLPLTAPKS HGFTAVILT YDRVDSLYT LIDRLSRVP 
      SLMKVIVVW NNQKKSPPP LSSFPQIQK PVKVIRTSA 
      NKLSNRFYP YNEIETEAI LHIDDDIVM LTADEVEFG 
      YEVWREFPD RIVGFPSRT HRWDNATNS WKYESEWTN 
      EISMVLTGA AFLHKYWSF LYTTAMPAN IRELVDEHM 
      NCEDIAMNF LVANVTNKP PIKVTPRKK FKCPECVNN 
      EMLSADEGH MIERSQCVD RFVKAFGRM PLKSVEFRA 
      DPVLFKDPF PEKLKRFND IGSL
    CDS sequence
    • >TC004910 RA
      atgactata catacaaaa atcgcaata tcgaaagtg 
      tacaactac acccacaat acttcttta tacaaaaat 
      ttctttttt cttgtgttt attgtactg attgtaact 
      acgttcacc gttttaccg agactacgt atcaatatt 
      ggtgatgag tcttcaaac ttcaagaaa atcactttg 
      gtaaacatc agaaacttg cccgaattt agattggta 
      ggggatagt gcctcgccg agaaaccca gaatgcacc 
      cattgggac tgcttcaat atttacaaa tgtgggcgc 
      acgggtcat gacaggata gccgtttac gtttacccg 
      ccgaaaaaa tacgtagat gaacaggga gtccctgca 
      actgaactc atgtcgaag gaatattta gctttactc 
      caggctgtg gtcaattcc aaatactac actgctaac 
      ccgcacgaa gcgtgcatt ttcattcca tccattgat 
      accttgaac caggaaaga ctcaggcca aacctcacc 
      tcaaaggct ttaagaagc ttgcctttc tggaatgat 
      ggagaaaat catctcatt ttcaatatg atagcagga 
      aatgctcct gatttttcc ccagttgtc gacttagcc 
      actggaaag gctctgata gctggagct gacttcaat 
      aaatactca tttaggacg aattttgat gtctcgata 
      ccgttattt agtccaatc gctagacct ggggatatt 
      aaccatcgt cgaaggtcg tggttggtg acgtcttcg 
      caactcaac ctcgacgtc aactacttg ggagttctc 
      cagaagcta tcatcgcgc cacaactcg cttttaatc 
      ctcgacgcc tgccataga cacaactac acgaaacgt 
      tgcagggag aattcaaac gaaatggtg gattatcct 
      gcgatttta cacgaaagc acattttgt ctcgttttt 
      cgaggcgaa cgcatcggt caattcgct ttattggaa 
      gcgatggcc gccaattgt atccccgtc gtggtaatg 
      gacggggca gtgttgcca tttagtaat gtgatagat 
      tggaaacgt gccgccgtt ttcatcatg gagaattat 
      ttacacacc ttagtggac gtactcgag aaaatttct 
      cctcaacgt atcaaacag atgcaaaaa actggccga 
      ttcctctac gattcgtac ttctcaagc attgagaaa 
      attgcactc acgacttta gatattatc caggatcgg 
      gtgtatcca cactggtcg aaaatttac gacgattgg 
      aatcttcga ccagaggag aaaaataca aatccgctg 
      tttttgccg ttgacggca ccgaaatcg cacggtttt 
      accgcggtt attttaacg tatgatcga gttgatagt 
      ctttacact ttgatcgat aggttatca cgagttcct 
      agtcttatg aaagttatc gttgtgtgg aacaaccaa 
      aagaaaagt ccgccgcca ttatcttcg tttcctcaa 
      atacagaag ccagtcaaa gtcattcga acaagtgcc 
      aataaatta tctaatcga ttttatccg tataatgag 
      atcgaaact gaggccatt ttacatatc gatgatgat 
      atcgttatg ttgacggca gacgaagtc gagttcggt 
      tatgaggtc tggagggag tttccagat cggatcgta 
      ggctttccc agtcgtact cacaggtgg gacaatgct 
      accaacagc tggaaatac gaatcggaa tggaccaat 
      gaaatttcg atggttctt actggcgca gctttccta 
      cacaagtat tggagcttt ctgtacacc acagcaatg 
      ccagcaaac attcgcgag ttggtagac gagcacatg 
      aattgtgag gacattgcc atgaatttt ctagttgca 
      aatgttaca aataagcca ccgattaag gttacacct 
      cgcaaaaag ttcaaatgt ccggaatgt gtcaataat 
      gaaatgctg tcggccgac gaaggtcat atgatagag 
      aggtcccag tgtgtggat agattcgtg aaagctttc 
      ggccgcatg cctttaaaa tcggtcgaa tttcgagca 
      gatcctgta ttattcaaa gatccattc ccagagaag 
      ttgaaacgc tttaacgac attggcagc ttgtag
    mRNA sequence
    • >TC004910 RA
      tcctgttgg atcaattgt tttttatgc gccctaacc 
      tcgaaataa tgtttatta gtgaaacgt caaagtagt 
      gttaaacat gacttaaat tagacgagt gtgcgaaat 
      cccaaagta aataaattg tgccatgta tttgacgag 
      gggccgcat caaactaaa gtgctaatc tttactcta 
      aaatgacta tacatacaa aaatcgcaa tatcgaaag 
      tgtacaact acacccaca atacttctt tatacaaaa 
      atttctttt ttcttgtgt ttattgtac tgattgtaa 
      ctacgttca ccgttttac cgagactac gtatcaata 
      ttggtgatg agtcttcaa acttcaaga aaatcactt 
      tggtaaaca tcagaaact tgcccgaat ttagattgg 
      taggggata gtgcctcgc cgagaaacc cagaatgca 
      cccattggg actgcttca atatttaca aatgtgggc 
      gcacgggtc atgacagga tagccgttt acgtttacc 
      cgccgaaaa aatacgtag atgaacagg gagtccctg 
      caactgaac tcatgtcga aggaatatt tagctttac 
      tccaggctg tggtcaatt ccaaatact acactgcta 
      acccgcacg aagcgtgca ttttcattc catccattg 
      ataccttga accaggaaa gactcaggc caaacctca 
      cctcaaagg ctttaagaa gcttgcctt tctggaatg 
      atggagaaa atcatctca ttttcaata tgatagcag 
      gaaatgctc ctgattttt ccccagttg tcgacttag 
      ccactggaa aggctctga tagctggag ctgacttca 
      ataaatact catttagga cgaattttg atgtctcga 
      taccgttat ttagtccaa tcgctagac ctggggata 
      ttaaccatc gtcgaaggt cgtggttgg tgacgtctt 
      cgcaactca acctcgacg tcaactact tgggagttc 
      tccagaagc tatcatcgc gccacaact cgcttttaa 
      tcctcgacg cctgccata gacacaact acacgaaac 
      gttgcaggg agaattcaa acgaaatgg tggattatc 
      ctgcgattt tacacgaaa gcacatttt gtctcgttt 
      ttcgaggcg aacgcatcg gtcaattcg ctttattgg 
      aagcgatgg ccgccaatt gtatccccg tcgtggtaa 
      tggacgggg cagtgttgc catttagta atgtgatag 
      attggaaac gtgccgccg ttttcatca tggagaatt 
      atttacaca ccttagtgg acgtactcg agaaaattt 
      ctcctcaac gtatcaaac agatgcaaa aaactggcc 
      gattcctct acgattcgt acttctcaa gcattgaga 
      aaattgcac tcacgactt tagatatta tccaggatc 
      gggtgtatc cacactggt cgaaaattt acgacgatt 
      ggaatcttc gaccagagg agaaaaata caaatccgc 
      tgtttttgc cgttgacgg caccgaaat cgcacggtt 
      ttaccgcgg ttattttaa cgtatgatc gagttgata 
      gtctttaca ctttgatcg ataggttat cacgagttc 
      ctagtctta tgaaagtta tcgttgtgt ggaacaacc 
      aaaagaaaa gtccgccgc cattatctt cgtttcctc 
      aaatacaga agccagtca aagtcattc gaacaagtg 
      ccaataaat tatctaatc gattttatc cgtataatg 
      agatcgaaa ctgaggcca ttttacata tcgatgatg 
      atatcgtta tgttgacgg cagacgaag tcgagttcg 
      gttatgagg tctggaggg agtttccag atcggatcg 
      taggctttc ccagtcgta ctcacaggt gggacaatg 
      ctaccaaca gctggaaat acgaatcgg aatggacca 
      atgaaattt cgatggttc ttactggcg cagctttcc 
      tacacaagt attggagct ttctgtaca ccacagcaa 
      tgccagcaa acattcgcg agttggtag acgagcaca 
      tgaattgtg aggacattg ccatgaatt ttctagttg 
      caaatgtta caaataagc caccgatta aggttacac 
      ctcgcaaaa agttcaaat gtccggaat gtgtcaata 
      atgaaatgc tgtcggccg acgaaggtc atatgatag 
      agaggtccc agtgtgtgg atagattcg tgaaagctt 
      tcggccgca tgcctttaa aatcggtcg aatttcgag 
      cagatcctg tattattca aagatccat tcccagaga 
      agttgaaac gctttaacg acattggca gcttgtaga 
      tccattttt gtagataat ttgtaaata tttatgtac 
      agattgtgt aaaccaggg ttaaattaa ttttttata 
      taaatttta gatttttta tccatgtgt atatgttgt 
      aaatcagat tatttttat ttatgttgt ttgtaactt 
      tgcttttat atgcgaaag atgacttat tgagggact 
      gacttaggt tagactcgc tgtttttta ctattaaaa 
      ctattaaat tatg
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