Status
Please wait ...
Gene information
General information
TC004891 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3815090..3817025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004891
    iBeetle screen iB_09591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004891 RA
      MTTLMMDYE FKMKTQNER VKVEDLFDY EGCKVGRGT 
      YGHVYKGRR KDGSDNRDY ALKQIEGTG LSMSACREI 
      ALLRELKHP NVITLIRVF LSHNDRKVW LLFDFAEHD 
      LWHIIKFHR AAKANKKPV MVPKGMVKS LLYQILDGI 
      HYLHSNWVL HRDLKPANI LVMGEGPER GRVKIADMG 
      FARLFNAPL KPLADLDPV VVTFWYRAP ELLLGARHY 
      TKAIDIWAI GCIFAELLT SEPIFHCRQ EDIKTSNPY 
      HHDQLDRIF NVMGFPLEK DWEDIKKMP EHPTLVKDF 
      KKQSYINCS LVKYMDRHK IKPDSKAFH LLQKLLLMD 
      PNKRITSEQ AMQDAYFSE DPLPTQDVF AGCPIPYPK 
      REFLTDDDQ EEKAESKQR QNQQQQQQQ QQQQSQQQQ 
      QQQQQQQQQ QSQQQQSGN GDNHGNPSK RVRLSGNAH 
      PGQVNPQAI PISQQQEFH QQQMMYNNG SQQQQNFQQ 
      RF
    CDS sequence
    • >TC004891 RA
      atgacaact ctcatgatg gactacgaa tttaaaatg 
      aagactcag aatgagcgg gtaaaggtg gaggatttg 
      ttcgattac gagggttgt aaggtaggt cggggcacg 
      tacggccat gtgtataaa gggcggcgg aaagacggg 
      tcggacaat cgagactat gccctaaaa cagatcgag 
      gggacagga ctatccatg tcagcatgc agagaaata 
      gcgctttta agggaattg aaacaccca aacgttatc 
      acgttaatt agagtcttt ttatctcac aatgataga 
      aaagtgtgg cttttattc gacttcgcc gaacacgac 
      ttgtggcac attattaaa tttcacaga gccgctaaa 
      gctaataag aaaccagtc atggtccct aaaggaatg 
      gtgaagtca ttgctttac caaatctta gacggaatt 
      cattatttg cactcaaac tgggtgtta cacagggac 
      ttaaaaccg gcaaatatt ctggttatg ggtgaaggg 
      ccggagaga ggacgtgtt aaaatcgca gatatgggt 
      tttgcgcgc ctttttaac gcaccattg aaacctcta 
      gctgatttg gaccctgtt gtggtcact ttttggtac 
      cgagcgcct gaattattg ctcggagct cgccattac 
      acgaaagca attgatatt tgggccatt ggatgcatt 
      tttgccgaa ctgttgacc tcagaacca atttttcat 
      tgtcggcaa gaagacatc aaaaccagc aatccgtac 
      catcatgac cagttagac aggattttt aacgttatg 
      ggattcccc cttgaaaag gactgggaa gatatcaaa 
      aaaatgccc gaacatccc acactagtt aaagatttt 
      aaaaaacaa agctatatc aactgttcg ttagtaaaa 
      tacatggat agacataag attaaacct gacagtaaa 
      gcgtttcac ttattacaa aagttatta ttaatggac 
      ccaaataaa cgaattact tcagaacaa gcgatgcaa 
      gatgcctac ttttctgaa gaccctcta cctacgcag 
      gacgttttt gcgggttgt ccaatccca tatcccaag 
      cgcgaattt ttgactgat gacgatcag gaagagaag 
      gctgaaagt aagcaacgt caaaaccag cagcaacag 
      caacaacaa caacagcag caatctcag caacagcaa 
      caacagcag cagcaacaa caacaacag caatcccaa 
      cagcaacaa tctggaaat ggggataat cacgggaat 
      ccctcaaaa cgtgtgaga ctgtcgggg aatgcccat 
      cccggtcaa gtcaatcca caagctatt ccaatatcg 
      caacaacag gagtttcat cagcagcag atgatgtat 
      aataacggg tcgcaacaa caacagaat tttcaacaa 
      agattttaa
    mRNA sequence
    • >TC004891 RA
      ttgcaatta attattaaa ttaacagta gatatttaa 
      aaattaaat ttcattttt tctttagtt tgtgcgttg 
      tttttgcgc atgctcgtc gcggttaga catttgaca 
      agacgttga cgtttgtca aaaacaaat tttgattaa 
      ttattatta tttacaaac attcctgtt cttcccaat 
      agtgaagtg caaatttgt tagggaatc accggtgtt 
      atcatataa acttggcac ttctcgcga agtgctcta 
      atttctgat caattgaat attatgaca actctcatg 
      atggactac gaatttaaa atgaagact cagaatgag 
      cgggtaaag gtggaggat ttgttcgat tacgagggt 
      tgtaaggta ggtcggggc acgtacggc catgtgtat 
      aaagggcgg cggaaagac gggtcggac aatcgagac 
      tatgcccta aaacagatc gaggggaca ggactatcc 
      atgtcagca tgcagagaa atagcgctt ttaagggaa 
      ttgaaacac ccaaacgtt atcacgtta attagagtc 
      tttttatct cacaatgat agaaaagtg tggctttta 
      ttcgacttc gccgaacac gacttgtgg cacattatt 
      aaatttcac agagccgct aaagctaat aagaaacca 
      gtcatggtc cctaaagga atggtgaag tcattgctt 
      taccaaatc ttagacgga attcattat ttgcactca 
      aactgggtg ttacacagg gacttaaaa ccggcaaat 
      attctggtt atgggtgaa gggccggag agaggacgt 
      gttaaaatc gcagatatg ggttttgcg cgccttttt 
      aacgcacca ttgaaacct ctagctgat ttggaccct 
      gttgtggtc actttttgg taccgagcg cctgaatta 
      ttgctcgga gctcgccat tacacgaaa gcaattgat 
      atttgggcc attggatgc atttttgcc gaactgttg 
      acctcagaa ccaattttt cattgtcgg caagaagac 
      atcaaaacc agcaatccg taccatcat gaccagtta 
      gacaggatt tttaacgtt atgggattc ccccttgaa 
      aaggactgg gaagatatc aaaaaaatg cccgaacat 
      cccacacta gttaaagat tttaaaaaa caaagctat 
      atcaactgt tcgttagta aaatacatg gatagacat 
      aagattaaa cctgacagt aaagcgttt cacttatta 
      caaaagtta ttattaatg gacccaaat aaacgaatt 
      acttcagaa caagcgatg caagatgcc tacttttct 
      gaagaccct ctacctacg caggacgtt tttgcgggt 
      tgtccaatc ccatatccc aagcgcgaa tttttgact 
      gatgacgat caggaagag aaggctgaa agtaagcaa 
      cgtcaaaac cagcagcaa cagcaacaa caacaacag 
      cagcaatct cagcaacag caacaacag cagcagcaa 
      caacaacaa cagcaatcc caacagcaa caatctgga 
      aatggggat aatcacggg aatccctca aaacgtgtg 
      agactgtcg gggaatgcc catcccggt caagtcaat 
      ccacaagct attccaata tcgcaacaa caggagttt 
      catcagcag cagatgatg tataataac gggtcgcaa 
      caacaacag aattttcaa caaagattt taaatttag 
      gtttaagac cttaataaa tttatataa atcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY