Status
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Gene information
General information
TC011119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 23603..25655
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011119
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09590

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011119 RA
      MSQEIVNKF KLPGRYAGS EKSVWVEYI QLAMDYKPL 
      NLGQGFPDY QSAPSHVTE ALANVAKSP NCLLHQYTR 
      GFGHPRLVS AIAKLYSHL IGREINPHS EVLTTLGAY 
      EALYSTILG HVDVGDEVI IIEPFFDCY EPMVKCAGG 
      IPRFIPLRL RQNFTGELP SSGDWILDQ NELEGLFND 
      KTKMIIVNT PHNPLGKVF KMAELEFIA NLCKKWNVL 
      CIADEVYEW IVYKPNKHI RIATLPGMW ERTITIGSA 
      GKSFSVTGW KLGWAYGPA HLMLNLQMI HQNCVYTGV 
      TPIQESFSL NSKFAPFDC VIIGGRGYE F
    • >TC011119 RB
      MSQEIVNKF KLPGRYAGS EKSVWVEYI QLAMDYKPL 
      NLGQGFPDY QSAPSHVTE ALANVAKSP NCLLHQYTR 
      GFGHPRLVS AIAKLYSHL IGREINPHS EVLTTLGAY 
      EALYSTILG HVDVGDEVI IIEPFFDCY EPMVKCAGG 
      IPRFIPLRL RQNFTGELP SSGDWILDQ NELEGLFND 
      KTKMIIVNT PHNPLGKVF KMAELEFIA NLCKKWNVL 
      CIADEVYEW IVYKPNKHI RIATLPGMW ERTITIGSA 
      GKSFSVTGW KLGWAYGPA HLMLNLQMI HQNCVYTGE 
      AVAMSFEKE IERLDKDEC YFKSISCEL LAKRDFTAK 
      FLCEVGMKP TIPEGGYFM VADWSPLES KVDLASESD 
      KYKDYRFTK WMTKNIGLQ GIPPTAFYS EKHKNLAEN 
      FVRYCYFKK DENLQKAAE ILHNWKCGK K
    CDS sequence
    • >TC011119 RA
      atgtcgcaa gaaatcgtt aacaagttc aaacttccg 
      ggacgttac gctggcagc gaaaaaagc gtgtgggtc 
      gagtacatc cagttggcc atggattac aaaccgctg 
      aacttgggt cagggattc cccgactac caatcagcg 
      cccagtcac gtgactgaa gctttagcc aacgttgcc 
      aaatcgccc aattgttta ctccaccaa tacacgcga 
      ggttttggg catccccgg ctcgtgtcc gccattgct 
      aaactttac tcgcatttg attggtcgt gagattaat 
      ccgcacagt gaggttttg acaacttta ggagcttat 
      gaagctttg tattcgaca attttggga cacgtggat 
      gtgggagat gaggttata ataattgag ccgttcttc 
      gattgttac gaaccgatg gtcaagtgt gctgggggg 
      attccccgg tttattccc ttgcgattg cgccaaaac 
      tttaccggg gaattaccg agctctgga gactggatt 
      ttggaccaa aatgaattg gaggggtta tttaatgat 
      aaaacaaag atgattatt gtaaatacg ccccataat 
      ccgttgggt aaagtgttc aaaatggcg gagctcgaa 
      ttcatagcc aacttatgc aaaaaatgg aacgttttg 
      tgcattgcg gatgaagtt tacgagtgg attgtttac 
      aaaccgaat aaacatatc agaattgca acgctgccg 
      ggaatgtgg gaacgcacg attacgatt ggctcagcc 
      ggtaagagt ttcagtgtc acggggtgg aaactgggt 
      tgggcctat ggccccgcc cacttgatg cttaaccta 
      caaatgatt caccaaaac tgtgtctac actggtgtt 
      acgccaatc caagaaagt ttttcttta aattcgaaa 
      ttcgcgcct tttgactgt gtgattata ggaggccgt 
      ggctatgag ttttga
    • >TC011119 RB
      atgtcgcaa gaaatcgtt aacaagttc aaacttccg 
      ggacgttac gctggcagc gaaaaaagc gtgtgggtc 
      gagtacatc cagttggcc atggattac aaaccgctg 
      aacttgggt cagggattc cccgactac caatcagcg 
      cccagtcac gtgactgaa gctttagcc aacgttgcc 
      aaatcgccc aattgttta ctccaccaa tacacgcga 
      ggttttggg catccccgg ctcgtgtcc gccattgct 
      aaactttac tcgcatttg attggtcgt gagattaat 
      ccgcacagt gaggttttg acaacttta ggagcttat 
      gaagctttg tattcgaca attttggga cacgtggat 
      gtgggagat gaggttata ataattgag ccgttcttc 
      gattgttac gaaccgatg gtcaagtgt gctgggggg 
      attccccgg tttattccc ttgcgattg cgccaaaac 
      tttaccggg gaattaccg agctctgga gactggatt 
      ttggaccaa aatgaattg gaggggtta tttaatgat 
      aaaacaaag atgattatt gtaaatacg ccccataat 
      ccgttgggt aaagtgttc aaaatggcg gagctcgaa 
      ttcatagcc aacttatgc aaaaaatgg aacgttttg 
      tgcattgcg gatgaagtt tacgagtgg attgtttac 
      aaaccgaat aaacatatc agaattgca acgctgccg 
      ggaatgtgg gaacgcacg attacgatt ggctcagcc 
      ggtaagagt ttcagtgtc acggggtgg aaactgggt 
      tgggcctat ggccccgcc cacttgatg cttaaccta 
      caaatgatt caccaaaac tgtgtctac actggtgag 
      gccgtggct atgagtttt gagaaagag atagagcga 
      ttagataag gacgagtgt tattttaaa tcgatttcg 
      tgcgaattg ctcgcgaaa cgtgatttt acagcgaaa 
      tttttatgc gaggttgga atgaagccg acgattccc 
      gaaggcggg tatttcatg gttgctgat tggtcgccg 
      ctagaatcc aaagtggat ctagcgtcc gaaagtgat 
      aaatacaag gattatcgc tttacgaaa tggatgacg 
      aaaaatata ggtttacag gggattccc ccaacggct 
      ttttacagc gagaagcat aaaaatcta gccgagaat 
      tttgtgcga tattgttac tttaaaaaa gatgagaat 
      ttacaaaaa gccgccgaa attttgcat aattggaag 
      tgtgggaag aaataa
    mRNA sequence
    • >TC011119 RA
      cattcattt tccataccc agaggagac aaaaaatac 
      acatttttt gtaacaaaa caattgtaa ctatcacaa 
      aatacattt tttcaatta attttttgt atttatttg 
      ccccgacct tgaaaaatc gagattaaa aaagtgagg 
      ttagattag ttaaagtca cctcaaagt cacccaatt 
      aatagtcaa attattcaa attaaacgc actttctta 
      tctatttta tcacagtga ttgtgcaaa aacattcac 
      tctcttcag atggtcaga aacgtccta aaattgtcc 
      caaaaaatg tcgcaagaa atcgttaac aagttcaaa 
      cttccggga cgttacgct ggcagcgaa aaaagcgtg 
      tgggtcgag tacatccag ttggccatg gattacaaa 
      ccgctgaac ttgggtcag ggattcccc gactaccaa 
      tcagcgccc agtcacgtg actgaagct ttagccaac 
      gttgccaaa tcgcccaat tgtttactc caccaatac 
      acgcgaggt tttgggcat ccccggctc gtgtccgcc 
      attgctaaa ctttactcg catttgatt ggtcgtgag 
      attaatccg cacagtgag gttttgaca actttagga 
      gcttatgaa gctttgtat tcgacaatt ttgggacac 
      gtggatgtg ggagatgag gttataata attgagccg 
      ttcttcgat tgttacgaa ccgatggtc aagtgtgct 
      ggggggatt ccccggttt attcccttg cgattgcgc 
      caaaacttt accggggaa ttaccgagc tctggagac 
      tggattttg gaccaaaat gaattggag gggttattt 
      aatgataaa acaaagatg attattgta aatacgccc 
      cataatccg ttgggtaaa gtgttcaaa atggcggag 
      ctcgaattc atagccaac ttatgcaaa aaatggaac 
      gttttgtgc attgcggat gaagtttac gagtggatt 
      gtttacaaa ccgaataaa catatcaga attgcaacg 
      ctgccggga atgtgggaa cgcacgatt acgattggc 
      tcagccggt aagagtttc agtgtcacg gggtggaaa 
      ctgggttgg gcctatggc cccgcccac ttgatgctt 
      aacctacaa atgattcac caaaactgt gtctacact 
      ggtgttacg ccaatccaa gaaagtttt tctttaaat 
      tcgaaattc gcgcctttt gactgtgtg attatagga 
      ggccgtggc tatgagttt tgagaaaga gatagagcg 
      attagataa ggacgagtg ttattttaa atcgatttc 
      gtgcgaatt gctcgcgaa acgtgattt tacagcgaa 
      atttttatg cgaggttgg aatgaagcc gacgattcc 
      cgaaggcgg gtatttcat ggttgctga ttggtcgcc 
      gctaggtac gaacatagc tgaatgatg tgattggtt 
      aattttggc ggtttttta gaatccaaa gtggatcta 
      gcgtccgaa agtgataaa tacaaggat tatcgcttt 
      acgaaatgg atgacgaaa aatataggt ttacagggg 
      attccccca acggctttt tacagcgag aagcataaa 
      aatctagcc gagaatttt gtgcgatat tgttacttt 
      aaaaaagat gagaattta caaaaagcc gccgaaatt 
      ttgcataat tggaagtgt gggaagaaa taatttgtg 
      aggta
    • >TC011119 RB
      cattcattt tccataccc agaggagac aaaaaatac 
      acatttttt gtaacaaaa caattgtaa ctatcacaa 
      aatacattt tttcaatta attttttgt atttatttg 
      ccccgacct tgaaaaatc gagattaaa aaagtgagg 
      ttagattag ttaaagtca cctcaaagt cacccaatt 
      aatagtcaa attattcaa attaaacgc actttctta 
      tctatttta tcacagtga ttgtgcaaa aacattcac 
      tctcttcag atggtcaga aacgtccta aaattgtcc 
      caaaaaatg tcgcaagaa atcgttaac aagttcaaa 
      cttccggga cgttacgct ggcagcgaa aaaagcgtg 
      tgggtcgag tacatccag ttggccatg gattacaaa 
      ccgctgaac ttgggtcag ggattcccc gactaccaa 
      tcagcgccc agtcacgtg actgaagct ttagccaac 
      gttgccaaa tcgcccaat tgtttactc caccaatac 
      acgcgaggt tttgggcat ccccggctc gtgtccgcc 
      attgctaaa ctttactcg catttgatt ggtcgtgag 
      attaatccg cacagtgag gttttgaca actttagga 
      gcttatgaa gctttgtat tcgacaatt ttgggacac 
      gtggatgtg ggagatgag gttataata attgagccg 
      ttcttcgat tgttacgaa ccgatggtc aagtgtgct 
      ggggggatt ccccggttt attcccttg cgattgcgc 
      caaaacttt accggggaa ttaccgagc tctggagac 
      tggattttg gaccaaaat gaattggag gggttattt 
      aatgataaa acaaagatg attattgta aatacgccc 
      cataatccg ttgggtaaa gtgttcaaa atggcggag 
      ctcgaattc atagccaac ttatgcaaa aaatggaac 
      gttttgtgc attgcggat gaagtttac gagtggatt 
      gtttacaaa ccgaataaa catatcaga attgcaacg 
      ctgccggga atgtgggaa cgcacgatt acgattggc 
      tcagccggt aagagtttc agtgtcacg gggtggaaa 
      ctgggttgg gcctatggc cccgcccac ttgatgctt 
      aacctacaa atgattcac caaaactgt gtctacact 
      ggtgaggcc gtggctatg agttttgag aaagagata 
      gagcgatta gataaggac gagtgttat tttaaatcg 
      atttcgtgc gaattgctc gcgaaacgt gattttaca 
      gcgaaattt ttatgcgag gttggaatg aagccgacg 
      attcccgaa ggcgggtat ttcatggtt gctgattgg 
      tcgccgcta gaatccaaa gtggatcta gcgtccgaa 
      agtgataaa tacaaggat tatcgcttt acgaaatgg 
      atgacgaaa aatataggt ttacagggg attccccca 
      acggctttt tacagcgag aagcataaa aatctagcc 
      gagaatttt gtgcgatat tgttacttt aaaaaagat 
      gagaattta caaaaagcc gccgaaatt ttgcataat 
      tggaagtgt gggaagaaa taatttgtg aggtaaata 
      atttgtaaa taaagaaat atttttcga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY