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Gene information
General information
TC032001
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005231
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1767560..1769815
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032001
    iBeetle screen iB_09589

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032001 RA
      MSRNLLYYL LLSNLYLSK LQNLAEVKL IANYVKKQE 
      YPQHVTLFT CWNKRTILT VYKQLAEKK IVLNVLTNH 
      WKINQTKLS QHTFLVGDT LCPQFNSLL SHAKLLQAP 
      NKWLVFDQN STVNTNDLL LDSNFAVAS QISNGRFHL 
      KLCYKRAPN ETIKFNEIG VFSNGFEYY NHFIPTRNR 
      SDLSGVNIT VSYVVTKPD YPFDVEDYR FRHLEAFSK 
      LSYAMVYPM LEMLNCTKK FIQRSSWGY KGANETQFV 
      GGMFGDIQN GTAEIGGTV SFYTVDRMS VVDYLSVTT 
      PSDLKFILR APPLSYVNN LFTLPFDTK VWYCLYFIV 
      GVTVLILYV IVRCESTYE NALERRNNI DNIKPKFFD 
      VVMLQIEAI TQQGSENEP KTMSGRIAV FIVFLVLMF 
      LYTSYSANI VVLLQSTSA NINTLQDLL NSKITLGVE 
      DVVYSHHYF ETQTEFTRK SIYEKKVAP KNQKSNFMT 
      TEMGIEKMK DEFFAFHVE TTAGYKQIM DTFQEHEKC 
      GLIEIDYLN VLYPSITIR KNSPYKEIV KVNFRKIYE 
      SGIRHRQLN RIYYKKPHC VGKGGSFKS VGIVDIYFS 
      VEIFAIGCF MALWLLLLE VLFKKKIKF LVQTTLSRD 
      NL
    CDS sequence
    • >TC032001 RA
      atgtctcgt aacttactg tactatttg ttactttca 
      aacttgtat ctttcaaaa ttacaaaac ttagccgaa 
      gtaaaatta atagctaat tatgtgaaa aagcaagaa 
      tatccccaa cacgtgacc ctattcact tgttggaat 
      aaacgaaca atattaaca gtatacaaa cagcttgca 
      gagaaaaaa attgtttta aacgtgctc acaaatcat 
      tggaaaata aatcaaacc aaactttca caacacaca 
      tttctagtc ggtgacaca ttgtgtcca caatttaat 
      tcactcctc agtcacgcc aaattatta caagctcca 
      aataaatgg ctagtcttc gatcaaaat tctactgta 
      aatacaaat gatctgctc ttggacagc aactttgca 
      gtggcttcg caaatttca aacggtcgg tttcatcta 
      aaactgtgc tacaaacgt gcaccaaat gaaacaatc 
      aagtttaac gaaattgga gttttttcg aatggtttt 
      gagtattat aatcacttt atacctact cgaaaccgt 
      tcagatcta agtggtgta aacataacg gtttcgtat 
      gtggtcact aaaccggat tatccattt gatgttgaa 
      gattatcga tttagacac cttgaagct tttagtaaa 
      cttagttat gctatggtt tatcccatg cttgagatg 
      ttgaattgt acgaaaaaa tttatacaa cgttcctca 
      tggggttat aaaggggca aacgaaacg cagtttgtt 
      ggaggaatg tttggtgat attcaaaac ggtactgct 
      gagattgga ggtactgtc tccttctac acagttgac 
      cgaatgagt gttgttgac tatctttcg gtaacaaca 
      ccaagtgat ttaaaattt atcctaaga gcaccgcct 
      ctgtcttac gtaaacaat ctcttcact ttgcctttc 
      gatacaaaa gtttggtac tgcctgtac ttcattgtt 
      ggagttaca gttttaatt ttgtatgta attgttcgc 
      tgtgaaagt acgtacgaa aacgcctta gaaagacga 
      aacaacatt gacaacatc aaaccaaaa ttcttcgat 
      gttgttatg ctccaaatt gaagccata acccagcaa 
      ggatcagaa aacgaaccc aaaacaatg tctggacga 
      attgccgtt tttatcgta ttcttagtc ttgatgttc 
      ttgtacact tcgtattca gcaaacatt gttgtgctt 
      ttacaatca acgtccgca aacatcaac acattacaa 
      gacttgctc aactcaaaa ataacttta ggggttgaa 
      gatgtggtt tacagccac cattatttt gagacgcaa 
      accgaattt acgagaaaa tcaatttat gaaaaaaaa 
      gtcgctccc aagaatcaa aaatcgaat tttatgacg 
      acagaaatg gggattgaa aagatgaaa gatgagttt 
      tttgccttc catgttgaa acaacagct ggatacaaa 
      caaattatg gatacgttt caagaacat gaaaaatgt 
      ggtttgatt gaaatcgat tatttgaat gttttgtat 
      ccaagtatt acgataagg aaaaattcg ccctataaa 
      gagattgtc aaagttaat tttagaaaa atctacgaa 
      agtgggata cgtcatcgg caattgaac cgaatttac 
      tataagaaa ccacactgt gtgggcaaa ggaggtagt 
      tttaaaagc gtcggaatt gtcgacatt tatttttct 
      gtagaaatt tttgctatt ggatgtttt atggcatta 
      tggttgtta ttgcttgaa gtattattc aaaaagaaa 
      atcaaattt ctagtgcag acaacactt tcaagagat 
      aatttataa
    mRNA sequence
    • >TC032001 RA
      ccatgagct gccatgtct cgtaactta ctgtactat 
      ttgttactt tcaaacttg tatctttca aaattacaa 
      aacttagcc gaagtaaaa ttaatagct aattatgtg 
      aaaaagcaa gaatatccc caacacgtg accctattc 
      acttgttgg aataaacga acaatatta acagtatac 
      aaacagctt gcagagaaa aaaattgtt ttaaacgtg 
      ctcacaaat cattggaaa ataaatcaa accaaactt 
      tcacaacac acatttcta gtcggtgac acattgtgt 
      ccacaattt aattcactc ctcagtcac gccaaatta 
      ttacaagct ccaaataaa tggctagtc ttcgatcaa 
      aattctact gtaaataca aatgatctg ctcttggac 
      agcaacttt gcagtggct tcgcaaatt tcaaacggt 
      cggtttcat ctaaaactg tgctacaaa cgtgcacca 
      aatgaaaca atcaagttt aacgaaatt ggagttttt 
      tcgaatggt tttgagtat tataatcac tttatacct 
      actcgaaac cgttcagat ctaagtggt gtaaacata 
      acggtttcg tatgtggtc actaaaccg gattatcca 
      tttgatgtt gaagattat cgatttaga caccttgaa 
      gcttttagt aaacttagt tatgctatg gtttatccc 
      atgcttgag atgttgaat tgtacgaaa aaatttata 
      caacgttcc tcatggggt tataaaggg gcaaacgaa 
      acgcagttt gttggagga atgtttggt gatattcaa 
      aacggtact gctgagatt ggaggtact gtctccttc 
      tacacagtt gaccgaatg agtgttgtt gactatctt 
      tcggtaaca acaccaagt gatttaaaa tttatccta 
      agagcaccg cctctgtct tacgtaaac aatctcttc 
      actttgcct ttcgataca aaagtttgg tactgcctg 
      tacttcatt gttggagtt acagtttta attttgtat 
      gtaattgtt cgctgtgaa agtacgtac gaaaacgcc 
      ttagaaaga cgaaacaac attgacaac atcaaacca 
      aaattcttc gatgttgtt atgctccaa attgaagcc 
      ataacccag caaggatca gaaaacgaa cccaaaaca 
      atgtctgga cgaattgcc gtttttatc gtattctta 
      gtcttgatg ttcttgtac acttcgtat tcagcaaac 
      attgttgtg cttttacaa tcaacgtcc gcaaacatc 
      aacacatta caagacttg ctcaactca aaaataact 
      ttaggggtt gaagatgtg gtttacagc caccattat 
      tttgagacg caaaccgaa tttacgaga aaatcaatt 
      tatgaaaaa aaagtcgct cccaagaat caaaaatcg 
      aattttatg acgacagaa atggggatt gaaaagatg 
      aaagatgag ttttttgcc ttccatgtt gaaacaaca 
      gctggatac aaacaaatt atggatacg tttcaagaa 
      catgaaaaa tgtggtttg attgaaatc gattatttg 
      aatgttttg tatccaagt attacgata aggaaaaat 
      tcgccctat aaagagatt gtcaaagtt aattttaga 
      aaaatctac gaaagtggg atacgtcat cggcaattg 
      aaccgaatt tactataag aaaccacac tgtgtgggc 
      aaaggaggt agttttaaa agcgtcgga attgtcgac 
      atttatttt tctgtagaa atttttgct attggatgt 
      tttatggca ttatggttg ttattgctt gaagtatta 
      ttcaaaaag aaaatcaaa tttctagtg cagacaaca 
      ctttcaaga gataattta taaccaaca aacaagcaa 
      actaaactt caatttttt tatttcctt gaagtagat 
      gcaggctga gtctgttat aagtaccaa tttttttag 
      tttatctac tgggtgtta gggaatggt ctcccaaga 
      attttaggg tgcgtttat gaagcaaaa tgtgtcgaa 
      aacctttat acaattttt tgataaaac aaattaaaa 
      aatataaac gtgacatta gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY