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Gene information
General information
TC004861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3284179..3286003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004861
    iBeetle screen iB_09587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004861 RA
      MCYGPFARI CHWGPLTAL GIIKLVTGM TIHCNGMWW 
      PPARSFGGF LNSVVFISC SGLTLYNFL SAMYHGPGY 
      LPQNWKPTN ETDCTYLQW CGVCHGFKA PRSHHCRKC 
      GRCVLKMDH HCPWINNCV GWGNHAHFT SFLAFATLG 
      CLHASIILG CSLYRALNR VHYLYYGSG KEPIVYLGL 
      YGIILCVLA LGFTIGVVI AVGMLLFFQ IRAIIRNRT 
      GIEDWIMEK ANYRRKARN ETFIFPYDL GVWRNIRQV 
      INFSCQPVG DGIFWPVVD TCDQFTLTR EQIEQKTEK 
      RQRTKLYKI IYPASGSWV PITQGLKVC CCPPCTDEA 
      RIRLEVGDT VSVTRWRKY WLFGEKLQD LPQAEESLY 
      RVRGWFPRR CAVEMIDGS EQSVKDKKI K
    CDS sequence
    • >TC004861 RA
      atgtgttac gggccgttc gctcgcatt tgccattgg 
      ggccccttg actgcgtta ggaatcatc aaattggtg 
      actgggatg acgatacac tgcaacgga atgtggtgg 
      ccccctgcg cgctccttc gggggtttt ctcaatagt 
      gttgttttt attagctgt tcgggcctg actttgtac 
      aatttttta tcggctatg tatcacggg ccgggttat 
      ttgccccaa aactggaaa cctacgaat gaaactgat 
      tgcacgtac ttacaatgg tgcggggtt tgtcacggg 
      tttaaggcc cccagatcg caccattgt cgcaaatgc 
      gggcgttgt gtcttaaaa atggatcat cactgccct 
      tggataaat aactgtgtt ggctggggg aaccacgca 
      cattttacc tcgttcctg gcttttgcc actttgggg 
      tgtttacat gcttcgatt attctgggc tgttcactt 
      tatagagct ttaaaccgt gtacattat ttatattac 
      ggatcaggg aaagaaccg attgtttat ttaggatta 
      tacggaatc atcctctgt gtccttgcg ttaggtttc 
      actatcggt gtggtaatt gcagtagga atgctcctc 
      ttcttccaa ataagagca atcattcgc aacaggacc 
      ggaatcgag gactggatc atggagaaa gctaactat 
      cggcgaaaa gctcgcaac gaaacgttt attttccct 
      tatgattta ggcgtgtgg cggaacatt cgacaagtg 
      atcaatttt agttgccaa ccagtcggc gatggaatt 
      ttttggcca gttgtcgac acttgcgac caattcact 
      ctaacaagg gagcaaatt gaacaaaaa accgaaaaa 
      agacaaaga accaaactt tacaaaatc atatatcca 
      gcatcaggt tcgtgggtt cccataacc caaggcttg 
      aaagtttgt tgttgtccg ccgtgcacc gacgaggcg 
      cgaattaga cttgaggtt ggagatacg gtctctgtg 
      actcgatgg cgaaagtat tggttgttt ggagaaaaa 
      cttcaagat ttgcctcag gctgaagaa tcgctctat 
      agggtaagg gggtggttc ccccggcgg tgtgccgtt 
      gagatgatt gatggcagt gaacaaagc gtgaaagat 
      aagaaaatt aaataa
    mRNA sequence
    • >TC004861 RA
      ttttttcga tgtaaaatg tgaggttat gtttgaatg 
      aaacaacaa atgtttgct ttttattta aacaaaaat 
      gtgttacgg gccgttcgc tcgcatttg ccattgggg 
      ccccttgac tgcgttagg aatcatcaa attggtgac 
      tgggatgac gatacactg caacggaat gtggtggcc 
      ccctgcgcg ctccttcgg gggttttct caatagtgt 
      tgtttttat tagctgttc gggcctgac tttgtacaa 
      ttttttatc ggctatgta tcacgggcc gggttattt 
      gccccaaaa ctggaaacc tacgaatga aactgattg 
      cacgtactt acaatggtg cggggtttg tcacgggtt 
      taaggcccc cagatcgca ccattgtcg caaatgcgg 
      gcgttgtgt cttaaaaat ggatcatca ctgcccttg 
      gataaataa ctgtgttgg ctgggggaa ccacgcaca 
      ttttacctc gttcctggc ttttgccac tttggggtg 
      tttacatgc ttcgattat tctgggctg ttcacttta 
      tagagcttt aaaccgtgt acattattt atattacgg 
      atcagggaa agaaccgat tgtttattt aggattata 
      cggaatcat cctctgtgt ccttgcgtt aggtttcac 
      tatcggtgt ggtaattgc agtaggaat gctcctctt 
      cttccaaat aagagcaat cattcgcaa caggaccgg 
      aatcgagga ctggatcat ggagaaagc taactatcg 
      gcgaaaagc tcgcaacga aacgtttat tttccctta 
      tgatttagg cgtgtggcg gaacattcg acaagtgat 
      caattttag ttgccaacc agtcggcga tggaatttt 
      ttggccagt tgtcgacac ttgcgacca attcactct 
      aacaaggga gcaaattga acaaaaaac cgaaaaaag 
      acaaagaac caaacttta caaaatcat atatccagc 
      atcaggttc gtgggttcc cataaccca aggcttgaa 
      agtttgttg ttgtccgcc gtgcaccga cgaggcgcg 
      aattagact tgaggttgg agatacggt ctctgtgac 
      tcgatggcg aaagtattg gttgtttgg agaaaaact 
      tcaagattt gcctcaggc tgaagaatc gctctatag 
      ggtaagggg gtggttccc ccggcggtg tgccgttga 
      gatgattga tggcagtga acaaagcgt gaaagataa 
      gaaaattaa ataatttaa caaatatca ggttttcta 
      gtcaaaatc gtagttaag ttttaatac ctttcagct 
      atgtgtacc gatttttta taaggtttt gttatgata 
      tgacggttg agtatgtgg tgaaaaggt tcatccaac 
      ttttttggc gaaattatc aatctattt gattcgcgt 
      ttttgttgt aattgtatt atagggact gtcgatagc 
      atttaacgg cgcagttct tactagaag ttgataatt 
      tcttcagaa aaaaattat atatgtatt tggaagttt 
      tttattcca atgaaacga tttatactg atggaacac 
      ttttattca cccatcttt aaaacctgc ccgtttctt 
      tgactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY