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Gene information
General information
TC004824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2448994..2457607
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004824
    iBeetle screen iB_09585

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004824 RA
      MILLFHWFT SFLYSLFRK RMKQRAPSS WSNFTITKL 
      NSPNHPLAQ RDAENDIKF DPPVYKQRY ERAVDILLD 
      EKWKNQVNK VVDFGCAEF GFFVFLKNR LSLSELLLI 
      DIDDLLLND YLYRVYPLN ADHLVGRPK PLTVNVYAG 
      SIAEPDPSL LNTDAVIAL EIIEHLYPD TLDALPYNI 
      FSYIRPKLV IVTTPNAEF NVLFTKLQK FRHADHKFE 
      WTREQFQSW ATNITSRFP SYTVQFDGV GLGPHGTDD 
      SIGCCSQLA VFIRKDVIC DTYEETCNV SNDSCGCNS 
      YGVCTYHSH SRKLPDYKL IASINYPYD VDTRTEDEK 
      ILDELKYRM HLFENSEEE FYNVETKCF QIPLNQLIY 
      HITKPFPPE PDIRKILLK YNYKIEECR NPITKKLES 
      CVIYEAEMD SGGSGSDTE ASGYGSDNK YNVDEGKFS 
      DWDENDLTW TSSTSKENE PAVSSKGVK TQLHLEVNE 
      SKNPQALFD SGYQKSPPD DSPQSKDQA VALDFKSLN 
      DRPNKLSHI LSVFDNFDK INELDAVRR EKKYFNFAS 
      NLPHREVMK KICQEIAKH PIAGPSRDP KKGKQLKKS 
      QSSDSDESV DDVKSITTC ILENSLNKI ECQDEDIRG 
      NLIQELIPE ENLEEIPVV EPILIVENG DLANNNRDL 
      EGNNYPAED VEQNDEIVD NQELINANN NDVEVAVAQ 
      AEEDEEENI EIEPPVDVE VAWSSREAL FDINSQVDL 
      LEDFEMEAS DVVVNGVSF PNSCVIVAE NNDPVLPPE 
      ESGFPNWLL QIFDEAEVL PPEDDLHDE PHFYCQGDG 
      LGVHPSTVA INDDDADDE GNDSGSDSS SSSGSVDFA 
      NADDHSVQE VFVADESRN DNSLVLVGQ PMSIQEPNE 
      EVETSDQED VFENAEGSE SNLVASSCT Q
    CDS sequence
    • >TC004824 RA
      atgatattg ttattccac tggtttaca agttttctc 
      tacagtttg tttagaaaa cgaatgaaa caaagagct 
      ccctcatcg tggagtaac ttcaccata accaagcta 
      aattcacca aaccatcct ttggctcaa cgtgacgcc 
      gaaaacgat ataaaattt gatcccccg gtgtacaaa 
      cagcgatac gaaagagca gtcgatatc cttttggac 
      gaaaaatgg aaaaaccaa gtaaataaa gtggtggat 
      tttggttgt gcggagttc ggtttcttt gtcttcctc 
      aagaacaga ctatcatta agcgaactg ttacttatc 
      gatattgac gatttgtta ctgaatgat tatctctac 
      agagtgtac cccttaaac gccgatcat ttggttgga 
      agaccaaaa cctttaact gtaaatgtt tacgcagga 
      agtattgca gaacccgac ccaagtctg ttaaacaca 
      gacgctgtt attgcatta gaaatcatt gaacacttg 
      tatcctgat acactagac gcgcttcca tacaacata 
      ttctcatac attagacca aaattagta attgtaaca 
      accccaaac gccgaattc aatgttttg tttacaaaa 
      ttgcaaaaa tttaggcat gctgatcac aaattcgaa 
      tggaccagg gagcagttc cagtcatgg gccaccaac 
      ataacatca cgatttcct tcatacaca gtccagttt 
      gatggtgtt ggcttaggc cctcatgga actgacgac 
      agcattggt tgttgctct caattagct gtgttcatc 
      agaaaagac gttatctgt gatacttac gaggaaact 
      tgcaatgtt tcaaacgat tcttgcggc tgtaattct 
      tatggtgtc tgtacatat cattcccac tctcgaaaa 
      ctaccagac tataaatta attgcatca attaattat 
      ccttacgat gttgacacg cggactgaa gacgaaaaa 
      atcttagat gagctcaaa tatagaatg catttattc 
      gaaaacagt gaagaggag ttttacaat gtcgaaact 
      aagtgtttc caaatacca cttaatcaa cttatttac 
      cacataaca aagccgttt ccaccagaa ccggatata 
      agaaaaata ttgttaaaa tataattac aaaattgaa 
      gaatgtcgg aatccaatt actaagaaa ctcgaatct 
      tgcgtaatt tatgaggct gaaatggac agtgggggc 
      tccggtagt gataccgaa gcgtcaggc tatggttca 
      gacaataaa tacaacgtt gatgagggc aagtttagc 
      gattgggac gaaaacgac ttaacgtgg accagctcc 
      acaagcaaa gagaatgaa ccggctgtg agtagcaaa 
      ggcgtcaaa acgcagctc cacttagaa gtcaacgag 
      agcaaaaac ccgcaagct ttattcgat tccggttac 
      caaaaatcg cctcctgac gactcgccc caatcaaaa 
      gaccaggcc gttgctcta gactttaaa agcttgaat 
      gatagaccg aacaaacta tcgcacatt ttatcggtg 
      tttgataat ttcgacaaa atcaacgaa ctagatgcg 
      gtccggcgg gaaaaaaag tacttcaat ttcgcctca 
      aatctgccg caccgcgaa gtgatgaaa aaaatttgc 
      caagaaatc gccaaacat ccaattgct ggaccttca 
      cgcgatcca aaaaaaggg aaacagctg aaaaagtca 
      caatcgtcg gatagtgac gaatcggtc gacgatgtc 
      aagtcgata acgacttgc attttggag aattcccta 
      aacaaaatt gagtgtcag gatgaggat atacggggg 
      aatttgata caagagttg ataccggag gagaatctg 
      gaagaaatt ccagttgtt gaaccaatt ttaatagta 
      gaaaacggc gatttagct aacaataac agggacttg 
      gagggtaat aattacccg gctgaagac gtggaacaa 
      aacgacgaa attgtggat aatcaagaa ttgatcaat 
      gctaataac aatgacgtg gaggtggcc gtggctcaa 
      gccgaggag gacgaagag gaaaacata gaaattgag 
      cctccggtt gacgtagaa gtggcttgg tccagccgt 
      gaagccctc tttgatata aactcgcaa gttgattta 
      cttgaagat tttgaaatg gaggcttcg gatgttgta 
      gtcaatggt gtttctttt ccaaacagt tgcgtaatt 
      gtggccgag aataatgat ccagtgcta cctcccgaa 
      gagagcggc tttcctaat tggttgttg cagattttt 
      gatgaggct gaggttttg ccgcctgaa gatgacctg 
      catgatgag cctcatttc tactgtcag ggtgacggg 
      ctaggggtt cacccatcc actgttgcc ataaatgac 
      gacgatgcg gacgacgag ggcaacgat tctggttca 
      gattcgtcg tcatcgagc ggttctgtt gattttgca 
      aacgctgat gatcattct gttcaagaa gttttcgtg 
      gccgatgag tcccgaaat gacaatagt ttggtacta 
      gtgggtcaa ccaatgtct attcaagaa ccaaatgaa 
      gaagtcgag acctcggac caagaagac gtgtttgaa 
      aacgccgag ggatcagaa tctaattta gttgctagt 
      tcttgtact caataa
    mRNA sequence
    • >TC004824 RA
      catgcgtca ttcttgact ttgtagaat aatataaac 
      aaaaacaaa atgctttga taacattta cgaaataca 
      gaaaaatga tattgttat tccactggt ttacaagtt 
      ttctctaca gtttgttta gaaaacgaa tgaaacaaa 
      gagctccct catcgtgga gtaacttca ccataacca 
      agctaaatt caccaaacc atcctttgg ctcaacgtg 
      acgccgaaa acgatataa aatttgatc ccccggtgt 
      acaaacagc gatacgaaa gagcagtcg atatccttt 
      tggacgaaa aatggaaaa accaagtaa ataaagtgg 
      tggattttg gttgtgcgg agttcggtt tctttgtct 
      tcctcaaga acagactat cattaagcg aactgttac 
      ttatcgata ttgacgatt tgttactga atgattatc 
      tctacagag tgtacccct taaacgccg atcatttgg 
      ttggaagac caaaacctt taactgtaa atgtttacg 
      caggaagta ttgcagaac ccgacccaa gtctgttaa 
      acacagacg ctgttattg cattagaaa tcattgaac 
      acttgtatc ctgatacac tagacgcgc ttccataca 
      acatattct catacatta gaccaaaat tagtaattg 
      taacaaccc caaacgccg aattcaatg ttttgttta 
      caaaattgc aaaaattta ggcatgctg atcacaaat 
      tcgaatgga ccagggagc agttccagt catgggcca 
      ccaacataa catcacgat ttccttcat acacagtcc 
      agtttgatg gtgttggct taggccctc atggaactg 
      acgacagca ttggttgtt gctctcaat tagctgtgt 
      tcatcagaa aagacgtta tctgtgata cttacgagg 
      aaacttgca atgtttcaa acgattctt gcggctgta 
      attcttatg gtgtctgta catatcatt cccactctc 
      gaaaactac cagactata aattaattg catcaatta 
      attatcctt acgatgttg acacgcgga ctgaagacg 
      aaaaaatct tagatgagc tcaaatata gaatgcatt 
      tattcgaaa acagtgaag aggagtttt acaatgtcg 
      aaactaagt gtttccaaa taccactta atcaactta 
      tttaccaca taacaaagc cgtttccac cagaaccgg 
      atataagaa aaatattgt taaaatata attacaaaa 
      ttgaagaat gtcggaatc caattacta agaaactcg 
      aatcttgcg taatttatg aggctgaaa tggacagtg 
      ggggctccg gtagtgata ccgaagcgt caggctatg 
      gttcagaca ataaataca acgttgatg agggcaagt 
      ttagcgatt gggacgaaa acgacttaa cgtggacca 
      gctccacaa gcaaagaga atgaaccgg ctgtgagta 
      gcaaaggcg tcaaaacgc agctccact tagaagtca 
      acgagagca aaaacccgc aagctttat tcgattccg 
      gttaccaaa aatcgcctc ctgacgact cgccccaat 
      caaaagacc aggccgttg ctctagact ttaaaagct 
      tgaatgata gaccgaaca aactatcgc acattttat 
      cggtgtttg ataatttcg acaaaatca acgaactag 
      atgcggtcc ggcgggaaa aaaagtact tcaatttcg 
      cctcaaatc tgccgcacc gcgaagtga tgaaaaaaa 
      tttgccaag aaatcgcca aacatccaa ttgctggac 
      cttcacgcg atccaaaaa aagggaaac agctgaaaa 
      agtcacaat cgtcggata gtgacgaat cggtcgacg 
      atgtcaagt cgataacga cttgcattt tggagaatt 
      ccctaaaca aaattgagt gtcaggatg aggatatac 
      gggggaatt tgatacaag agttgatac cggaggaga 
      atctggaag aaattccag ttgttgaac caattttaa 
      tagtagaaa acggcgatt tagctaaca ataacaggg 
      acttggagg gtaataatt acccggctg aagacgtgg 
      aacaaaacg acgaaattg tggataatc aagaattga 
      tcaatgcta ataacaatg acgtggagg tggccgtgg 
      ctcaagccg aggaggacg aagaggaaa acatagaaa 
      ttgagcctc cggttgacg tagaagtgg cttggtcca 
      gccgtgaag ccctctttg atataaact cgcaagttg 
      atttacttg aagattttg aaatggagg cttcggatg 
      ttgtagtca atggtgttt cttttccaa acagttgcg 
      taattgtgg ccgagaata atgatccag tgctacctc 
      ccgaagaga gcggctttc ctaattggt tgttgcaga 
      tttttgatg aggctgagg ttttgccgc ctgaagatg 
      acctgcatg atgagcctc atttctact gtcagggtg 
      acgggctag gggttcacc catccactg ttgccataa 
      atgacgacg atgcggacg acgagggca acgattctg 
      gttcagatt cgtcgtcat cgagcggtt ctgttgatt 
      ttgcaaacg ctgatgatc attctgttc aagaagttt 
      tcgtggccg atgagtccc gaaatgaca atagtttgg 
      tactagtgg gtcaaccaa tgtctattc aagaaccaa 
      atgaagaag tcgagacct cggaccaag aagacgtgt 
      ttgaaaacg ccgagggat cagaatcta atttagttg 
      ctagttctt gtactcaat aaatctcaa accaagtgt 
      agttatttt catttgtaa cgagactga acgcttttg 
      taatgaata aaacttgtg tttctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY