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Gene information
General information
TC031957
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004539
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2918992..2920286
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031957
    iBeetle screen iB_09581

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031957 RA
      MLVKPALFC ALVALVASK SSPSVTFKN VLVSGYSMY 
      TYKFNVTIK SSNNLKENL PKQVFDRLD IINSEIPVL 
      YKNSISDLE DLDELIFAN NELSDIQPG AFKNVPQLR 
      KLEIRNNKL EEIKAGVFN NLNIATLDL SNNSISTIA 
      SGAFDDMPE LLNVNLADN RVKIWNKNW FANTPLLAR 
      VSFQNNLIE EIPSNAFRH FQGEKQFGS VPLTINIVL 
      SHNLIKHIH NDAFKGLEK INNLWLDGN ELSEWDENL 
      LTGVSVHDL RIDNNKLEC LDGNLDKVF VAATTHIDG 
      NPWDCGCLK NIDLWASKA TQHVVMIYS KMNCDAERI 
      ARKLDALDL RLKELRKNN TDVM
    CDS sequence
    • >TC031957 RA
      atgttagta aaaccggcg ttattttgt gccttggtg 
      gctttagtg gccagcaaa tcgagtcca agtgttaca 
      ttcaaaaat gtccttgtt agtggctac agcatgtac 
      acttacaaa ttcaacgtg accataaaa tcatcgaac 
      aatttgaaa gaaaatctc ccaaaacaa gtgtttgat 
      cgacttgac ataatcaac agcgaaatc cccgtcttg 
      tacaaaaat tcaatttcg gatctcgaa gacttggac 
      gaactaatt tttgcgaac aacgagctt tcggacatc 
      cagcctgga gctttcaaa aacgtccct cagttgcgc 
      aaattggaa atacgaaac aataagttg gaagaaatc 
      aaagctgga gttttcaac aatttgaac attgccact 
      ttggatttg agtaacaat tccataagt acgatagct 
      tcgggcgct ttcgacgac atgcccgag cttttgaat 
      gtgaacctt gccgacaac cgagtgaag atttggaac 
      aagaactgg tttgccaac accccactg ttggcaagg 
      gtctcgttc cagaataat ttaatcgag gagattccg 
      tccaacgct tttcgacat ttccagggc gagaagcag 
      ttcggttcg gtgcctttg accatcaat atagtgctg 
      agccacaac ttgatcaaa cacatccat aacgacgcc 
      ttcaagggc ttggagaag atcaacaat ttgtggtta 
      gatggtaac gagttgagc gaatgggac gaaaatttg 
      ttaaccgga gtttcggtg catgattta agaattgat 
      aataataag ttggagtgt ctggacggt aatttggac 
      aaggttttc gttgctgct acaactcat atcgatggt 
      aatccgtgg gattgtggg tgtttgaaa aatattgat 
      ttgtgggcc tctaaagca acgcaacac gtggttatg 
      atttattca aaaatgaac tgcgatgct gaacgaatc 
      gctcggaaa ttggacgct ttggacctc cggctcaag 
      gaattgagg aaaaataat actgacgta atgtaa
    mRNA sequence
    • >TC031957 RA
      ctagtataa aaactggag gaaacgaaa cgtaaagta 
      cagtgttac aatgttagt aaaaccggc gttattttg 
      tgccttggt ggctttagt ggccagcaa atcgagtcc 
      aagtgttac attcaaaaa tgtccttgt tagtggcta 
      cagcatgta cacttacaa attcaacgt gaccataaa 
      atcatcgaa caatttgaa agaaaatct cccaaaaca 
      agtgtttga tcgacttga cataatcaa cagcgaaat 
      ccccgtctt gtacaaaaa ttcaatttc ggatctcga 
      agacttgga cgaactaat ttttgcgaa caacgagct 
      ttcggacat ccagcctgg agctttcaa aaacgtccc 
      tcagttgcg caaattgga aatacgaaa caataagtt 
      ggaagaaat caaagctgg agttttcaa caatttgaa 
      cattgccac tttggattt gagtaacaa ttccataag 
      tacgatagc ttcgggcgc tttcgacga catgcccga 
      gcttttgaa tgtgaacct tgccgacaa ccgagtgaa 
      gatttggaa caagaactg gtttgccaa caccccact 
      gttggcaag ggtctcgtt ccagaataa tttaatcga 
      ggagattcc gtccaacgc ttttcgaca tttccaggg 
      cgagaagca gttcggttc ggtgccttt gaccatcaa 
      tatagtgct gagccacaa cttgatcaa acacatcca 
      taacgacgc cttcaaggg cttggagaa gatcaacaa 
      tttgtggtt agatggtaa cgagttgag cgaatggga 
      cgaaaattt gttaaccgg agtttcggt gcatgattt 
      aagaattga taataataa gttggagtg tctggacgg 
      taatttgga caaggtttt cgttgctgc tacaactca 
      tatcgatgg taatccgtg ggattgtgg gtgtttgaa 
      aaatattga tttgtgggc ctctaaagc aacgcaaca 
      cgtggttat gatttattc aaaaatgaa ctgcgatgc 
      tgaacgaat cgctcggaa attggacgc tttggacct 
      ccggctcaa ggaattgag gaaaaataa tactgacgt 
      aatgtaatt gtcttgttt acaatcgta atttcttat 
      tcttgttat gtaaataaa taaaaggca gtcgagttg 
      cattcagtt cctgttcgt cctcaaatc cagacatgt 
      ctaagttta ttcctgtgg gtttgtgtt tggttgctt 
      gtgttatca gtcaaagtg atgaacatt tccgaacaa 
      ataatttga cttttatgg tggagccta aaggcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY