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Gene information
General information
TC002097 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452015.1: 4899..7003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002097
    iBeetle screen iB_09580

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002097 RA
      MLKTVQMVQ LMETTHKNL WLKLTPYLA LFIFIDLSE 
      CINQAEVDR HLELGKQYL ARGQLGDAL SHYHSAVEG 
      DPHNYLTYF KRGTVYLAL GKAKNAISD FDLVLEMKP 
      DFTAARISR GNIHLKQAN YDLAQLDFY NVLKTDPYN 
      QEANDLIAR IDPAKERVR LAEYYYAHD DYVNAIHLV 
      TEALETSPW APQLYELRS QMHMHNGDE MAAVSDIRS 
      ATKLQSDNT EGYFKLSQL LYKLGHASE SLKFIRECL 
      KLDPEHKDC FPFYKKIKK VEKFLTEAE AALENKNYQ 
      ECIDAALKA EKNEKEVQN VVYNAKRLL CACYSNDEQ 
      SEEAVTACT QALEINDDP NVYCDRAEA YLQSELYDD 
      AIRDYKAAL EMNSQFERA REGLSKAEN RQKQSERRD 
      YYKILGVKR TASKREITK AYRKMAQKW HPDNYQNDE 
      KMKKIAEKK FIDIAAAKE VLTDDEKRR QFDNGEDPL 
      DPESGRGGM PNFHQFHTF HGSPFQFKF HFN
    CDS sequence
    • >TC002097 RA
      atgttaaag acagtacaa atggtacaa ttaatggaa 
      acgacacat aaaaacctc tggttgaag ttaacaccg 
      tatttagcg ctatttatc tttattgac ctttcggaa 
      tgtatcaat caggccgaa gttgaccgg catttagag 
      ttagggaaa caatactta gctaggggc caattaggg 
      gacgccttg agccattat cattcggct gttgagggc 
      gaccctcac aattattta acttatttc aaacgcggc 
      acggtttat ctcgcttta ggcaaggcc aagaatgca 
      atcagcgac tttgacttg gtcctggag atgaaacca 
      gacttcacc gctgctagg attagtcga ggaaatatt 
      cacttaaaa caggccaat tatgacctg gcccagtta 
      gatttttat aatgtgtta aaaacagac ccttacaac 
      caagaggcc aatgatttg attgcacgg atagaccca 
      gccaaggag cgagtgcgt ttggctgaa tattattac 
      gcccatgat gattacgta aacgcaatt cacttagtc 
      acggaggcc ctagagaca agcccctgg gccccccag 
      ttgtacgag ctgcggtcc cagatgcat atgcacaac 
      ggtgacgaa atggcagct gtcagtgat atcagatcg 
      gccacaaag ctacaaagc gataacacc gaaggttac 
      ttcaagtta tcgcaatta ttatacaaa ctggggcat 
      gcttccgag tcccttaaa ttcataagg gaatgtctc 
      aaacttgat cccgaacac aaagattgc ttcccattt 
      tataaaaaa atcaaaaaa gttgagaaa ttcctaact 
      gaggctgag gcagctctt gagaacaag aattatcag 
      gagtgcatt gatgctgct ctcaaggct gaaaaaaac 
      gaaaaggag gttcagaat gttgtgtac aatgccaag 
      cggttgttg tgcgcttgt tattcgaat gatgagcag 
      tcggaggag gcggtgacg gcctgtact caagcgctt 
      gaaataaac gacgaccca aatgtttac tgtgatagg 
      gcagaggcg tatttacag tcagaactg tacgatgac 
      gctattagg gactacaag gcggcgttg gaaatgaat 
      tcgcagttt gaaagggcg agggaaggg ctcagcaag 
      gccgaaaat cgccaaaaa cagtcagaa cgtcgcgac 
      tattataaa atcctaggt gttaaaagg acagcttcc 
      aagcgggag atcacgaaa gcgtaccga aaaatggca 
      caaaagtgg caccccgac aattaccag aacgacgag 
      aagatgaag aaaattgcc gagaagaag tttattgac 
      attgctgcc gctaaagag gttttgacg gacgatgag 
      aagcgaaga cagtttgat aacggggaa gacccgctt 
      gaccctgag tcaggtcgg ggaggaatg cccaacttc 
      catcagttc cacactttc cacggaagt ccgttccag 
      ttcaaattt catttcaat taa
    mRNA sequence
    • >TC002097 RA
      cgaaacgta aatttgttg tgttcgtgt atgtcacaa 
      aatggaata aaatcgttc gttaagtgg gtgtaaata 
      atgttaaag acagtacaa atggtacaa ttaatggaa 
      acgacacat aaaaacctc tggttgaag ttaacaccg 
      tatttagcg ctatttatc tttattgac ctttcggaa 
      tgtatcaat caggccgaa gttgaccgg catttagag 
      ttagggaaa caatactta gctaggggc caattaggg 
      gacgccttg agccattat cattcggct gttgagggc 
      gaccctcac aattattta acttatttc aaacgcggc 
      acggtttat ctcgcttta ggcaaggcc aagaatgca 
      atcagcgac tttgacttg gtcctggag atgaaacca 
      gacttcacc gctgctagg attagtcga ggaaatatt 
      cacttaaaa caggccaat tatgacctg gcccagtta 
      gatttttat aatgtgtta aaaacagac ccttacaac 
      caagaggcc aatgatttg attgcacgg atagaccca 
      gccaaggag cgagtgcgt ttggctgaa tattattac 
      gcccatgat gattacgta aacgcaatt cacttagtc 
      acggaggcc ctagagaca agcccctgg gccccccag 
      ttgtacgag ctgcggtcc cagatgcat atgcacaac 
      ggtgacgaa atggcagct gtcagtgat atcagatcg 
      gccacaaag ctacaaagc gataacacc gaaggttac 
      ttcaagtta tcgcaatta ttatacaaa ctggggcat 
      gcttccgag tcccttaaa ttcataagg gaatgtctc 
      aaacttgat cccgaacac aaagattgc ttcccattt 
      tataaaaaa atcaaaaaa gttgagaaa ttcctaact 
      gaggctgag gcagctctt gagaacaag aattatcag 
      gagtgcatt gatgctgct ctcaaggct gaaaaaaac 
      gaaaaggag gttcagaat gttgtgtac aatgccaag 
      cggttgttg tgcgcttgt tattcgaat gatgagcag 
      tcggaggag gcggtgacg gcctgtact caagcgctt 
      gaaataaac gacgaccca aatgtttac tgtgatagg 
      gcagaggcg tatttacag tcagaactg tacgatgac 
      gctattagg gactacaag gcggcgttg gaaatgaat 
      tcgcagttt gaaagggcg agggaaggg ctcagcaag 
      gccgaaaat cgccaaaaa cagtcagaa cgtcgcgac 
      tattataaa atcctaggt gttaaaagg acagcttcc 
      aagcgggag atcacgaaa gcgtaccga aaaatggca 
      caaaagtgg caccccgac aattaccag aacgacgag 
      aagatgaag aaaattgcc gagaagaag tttattgac 
      attgctgcc gctaaagag gttttgacg gacgatgag 
      aagcgaaga cagtttgat aacggggaa gacccgctt 
      gaccctgag tcaggtcgg ggaggaatg cccaacttc 
      catcagttc cacactttc cacggaagt ccgttccag 
      ttcaaattt catttcaat taaatattt tgtgttgtg 
      tggccaact caggataag tgtgtgata aaaatccat 
      cacggaatt tgccagaac aagcttatc tagttattt 
      tgtgtaaaa agggactga tatcatgta cataaatta 
      aatgagtag tttttacca tttgtcctt acattgtac 
      atatttata tgataataa aatatttat attacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY