Status
Please wait ...
Gene information
General information
TC005295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450381.1: 735..8245
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005295
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_09579

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005295 RA
        MVEERLTGL ALLHVHKNI PIEADEVIT RFGRRPAEQ 
        TKTASTSVP LATTTTTAV NNAATVTLT PAAAAVTSA 
        SAKHTTTVN TATPTNTTT VVATILVAV AAATVTTAA 
        ILTKHPRTI TPTPDTATV GARPPTSRT STVTTQTEP 
        PEITIPETR LLTVPPPTV LIPVCPSCS FNPLLTPKT 
        PFLYFPPPP PRPRLPTPP PMPPTPPST EPTPKKATA 
        NTLLQPTFR WQDVNHRQV RAGKYYRIS PTVRCHPGP 
        NVGRNGRMK DINDQIIID YLNEQEYPV NETIRIKNR 
        KGETTSMML ISLDRKYKS IYNVKKIIG LDVVIEPLK 
        PKGENVQCH RCQRYGHVQ KYCNAQFKC MKCAEEHST 
        HECPKERTS TPKCAKCGG EHISIWRNC PTKRKIFAA 
        YSPPKAEID ENELDIIMN GNTPTLCAG DFNSKNKNW 
        KCKSDNKKR KDLAKYTEN RNLTVIAPT EPTHIPSNG 
        NADILDIAI IKNITENID CEILEHLNS DHYPVIIEI 
        SNSSIVKDS KIELHDINQ FKQNIKIPQ QEIRSIEDL 
        EKAVEDFEK EIITAWEKS KYTVKPRSQ RIPQEIILK 
        IQKTQTQKT LPTHSITPN KTRIEQNAT RSKRKITRK 
        AKRIVGPQT GKNK
      CDS sequence
      • >TC005295 RA
        atggttgaa gaacgtcta accggactg gcacttctc 
        cacgtccat aaaaacata cccattgaa gcagatgaa 
        gtgataacg cgttttggg agaagaccc gccgaacag 
        accaaaacc gcctctacc tccgtcccc ctagccacc 
        actaccacc actgccgtc aacaatgcc gccaccgtt 
        accctcact cccgccgcc gccgccgtt acctccgct 
        tccgccaaa cacaccacc accgtcaac accgccacc 
        cccaccaac actaccacc gtcgtcgcc accattctg 
        gtcgccgtt gccgccgcc accgtaacc accgccgcc 
        atcctcact aaacaccct cgcaccatc accccaaca 
        cccgacact gccaccgtg ggagcacgg ccacccacc 
        tcacgcact tccacggtg accacccag actgaaccc 
        cccgaaatt actataccc gagactagg ctcctcact 
        gtccctccc ccaaccgtc ctcatccca gtctgcccc 
        tcatgctct ttcaatccc ctgctaacc cctaaaact 
        cctttcctg tattttcca ccaccacca cctcgtcca 
        cgactccca acaccacca ccaatgccg ccaacccca 
        ccatcaact gaaccaacc ccaaaaaaa gcaacggcc 
        aatacgctt ctccaaccg acatttaga tggcaagac 
        gtcaaccac cgtcaagtc cgagctggg aagtattat 
        cggataagc cccacagtc aggtgtcat cccgggccg 
        aacgttggg agaaatgga agaatgaaa gacatcaac 
        gaccagata ataatagat tacctcaat gaacaagaa 
        tacccagta aatgaaacc atcaggata aagaacaga 
        aaaggagaa acaacatca atgatgcta atatcactc 
        gatcgaaaa tataaatcc atctacaat gtgaaaaag 
        ataatcgga ctagatgta gtcatcgaa cctctaaaa 
        ccaaaagga gaaaatgta cagtgccat cgttgtcaa 
        agatacggt cacgttcag aaatactgc aatgctcag 
        ttcaaatgt atgaagtgt gcggaagag cactcgacc 
        catgaatgt ccaaaagaa cgcacatcc acaccaaaa 
        tgtgctaag tgtggcggg gaacatatt tctatctgg 
        agaaactgc cctacaaaa agaaaaatc ttcgcagca 
        tacagccct ccaaaagca gaaatcgac gaaaatgaa 
        cttgacatt atcatgaac ggaaatacc ccaactctt 
        tgcgctggc gacttcaac agcaagaac aagaactgg 
        aaatgcaaa agcgacaac aaaaaaaga aaagatcta 
        gcaaaatac acggagaac agaaaccta actgtaatt 
        gcaccaaca gaaccaaca cacatccct tcaaacgga 
        aatgctgac atactggat atagcaata ataaaaaat 
        ataacagaa aatattgat tgcgaaatc ctagaacac 
        ctaaactca gaccactac cctgtaatc atagaaata 
        agcaacagc tcaattgta aaagacagt aaaatagaa 
        ctacacgac ataaaccaa tttaaacaa aatataaaa 
        attcctcaa caagaaatt agaagcata gaagatcta 
        gaaaaagca gtagaagac tttgaaaaa gaaataata 
        acagcttgg gaaaaatcg aaatacaca gtaaaacca 
        agaagccaa cgaatccca caagaaatc atattgaaa 
        atccaaaaa acgcaaact cagaaaact ctaccaact 
        cactctatc accccaaat aaaacaaga attgaacaa 
        aatgcaaca agaagtaaa agaaaaatt acaagaaaa 
        gctaaacga atcgtggga ccgcaaact ggaaaaaat 
        aaatag
      mRNA sequence
      • >TC005295 RA
        cttacctca atcaactgt tttattctc ataatagtt 
        aatacagta ctaagttaa aaaacttta aaatttcat 
        tataagtaa tagcgttct taaaagtgg ttggtgcaa 
        attcattac aagaaatgg cgctactgt atctttaag 
        cagaatact gcaatcgaa ctcaattga tttaaaaaa 
        agctgcgga cgctattaa tgatactat aagtgttct 
        acaaaataa aagagaaaa tgcggacgt tatttaccg 
        acagctttt tgaagaggc taaagaagt ggctgggca 
        gctagatgt agaaattaa atgtcccag aatagtgtc 
        aagacaaat acatagggc caataacca acctgccca 
        atctgctga agagtattt ccgcagagc tatatacat 
        ccctcttct agattcaat aataaatga cctagagga 
        ccgtatgtc tcttgaagt acttaattt gttcagctc 
        agcgttttc atgccaaaa agcgaatac agtaatcaa 
        gacattgaa actgtgaaa caactagca accgattat 
        acattgtta ctagataat actccagtt tctgtcgtt 
        gtaaacgaa tatcgactg tggatggtg aagtggcag 
        cggagtcag gacatgccg cagtcgatt tctgacctt 
        attttaaat tgcgatatt gaaatgtat cctaatata 
        agaaaattt ttatgtatt atggctaca ctacctgtt 
        agtatagcg acagcggaa agatctttc tctacatta 
        cgaagaatc aaatcatgg ctaagagct gcaatggtt 
        gaagaacgt ctaaccgga ctggcactt ctccacgtc 
        cataaaaac atacccatt gaagcagat gaagtgata 
        acgcgtttt gggagaaga cccgccgaa cagaccaaa 
        accgcctct acctccgtc cccctagcc accactacc 
        accactgcc gtcaacaat gccgccacc gttaccctc 
        actcccgcc gccgccgcc gttacctcc gcttccgcc 
        aaacacacc accaccgtc aacaccgcc acccccacc 
        aacactacc accgtcgtc gccaccatt ctggtcgcc 
        gttgccgcc gccaccgta accaccgcc gccatcctc 
        actaaacac cctcgcacc atcacccca acacccgac 
        actgccacc gtgggagca cggccaccc acctcacgc 
        acttccacg gtgaccacc cagactgaa ccccccgaa 
        attactata cccgagact aggctcctc actgtccct 
        cccccaacc gtcctcatc ccagtctgc ccctcatgc 
        tctttcaat cccctgcta acccctaaa actcctttc 
        ctgtatttt ccaccacca ccacctcgt ccacgactc 
        ccaacacca ccaccaatg ccgccaacc ccaccatca 
        actgaacca accccaaaa aaagcaacg gccaatacg 
        cttctccaa ccgacattt agatggcaa gacgtcaac 
        caccgtcaa gtccgagct gggaagtat tatcggata 
        agccccaca gtcaggtgt catcccggg ccgaacgtt 
        gggagaaat ggaagaatg aaagacatc aacgaccag 
        ataataata gattacctc aatgaacaa gaataccca 
        gtaaatgaa accatcagg ataaagaac agaaaagga 
        gaaacaaca tcaatgatg ctaatatca ctcgatcga 
        aaatataaa tccatctac aatgtgaaa aagataatc 
        ggactagat gtagtcatc gaacctcta aaaccaaaa 
        ggagaaaat gtacagtgc catcgttgt caaagatac 
        ggtcacgtt cagaaatac tgcaatgct cagttcaaa 
        tgtatgaag tgtgcggaa gagcactcg acccatgaa 
        tgtccaaaa gaacgcaca tccacacca aaatgtgct 
        aagtgtggc ggggaacat atttctatc tggagaaac 
        tgccctaca aaaagaaaa atcttcgca gcatacagc 
        cctccaaaa gcagaaatc gacgaaaat gaacttgac 
        attatcatg aacggaaat accccaact ctttgcgct 
        ggcgacttc aacagcaag aacaagaac tggaaatgc 
        aaaagcgac aacaaaaaa agaaaagat ctagcaaaa 
        tacacggag aacagaaac ctaactgta attgcacca 
        acagaacca acacacatc ccttcaaac ggaaatgct 
        gacatactg gatatagca ataataaaa aatataaca 
        gaaaatatt gattgcgaa atcctagaa cacctaaac 
        tcagaccac taccctgta atcatagaa ataagcaac 
        agctcaatt gtaaaagac agtaaaata gaactacac 
        gacataaac caatttaaa caaaatata aaaattcct 
        caacaagaa attagaagc atagaagat ctagaaaaa 
        gcagtagaa gactttgaa aaagaaata ataacagct 
        tgggaaaaa tcgaaatac acagtaaaa ccaagaagc 
        caacgaatc ccacaagaa atcatattg aaaatccaa 
        aaaacgcaa actcagaaa actctacca actcactct 
        atcacccca aataaaaca agaattgaa caaaatgca 
        acaagaagt aaaagaaaa attacaaga aaagctaaa 
        cgaatcgtg ggaccgcaa actggaaaa aataaatag 
        tcaggaaga aatccctgg aaaattgca aaattcctg 
        aaaaacgaa agagcacaa attccatcg ctaaaaaat 
        ggcatccaa acagctata accgacgaa gaaaaagcc 
        gacatattc aaaaaccaa ctaatcgag caatttact 
        cccaatgaa agcaataac ccagcctac gaagaaaga 
        ataaaccaa attacccac agaatactc aggacccca 
        gaaaactac aaattcgac aaaataaaa gcagaagaa 
        atataacaa ataataaaa accttaccg aaaagaaaa 
        acaccagga cgcgacaga ataaacaac gacataatc 
        aaaaaccta ccaccagat gcaataaat tatataata 
        taat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY