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Gene information
General information
TC031934
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001426,TC001427
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450792.1: 21665..26161
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031934
      iBeetle screen iB_09578

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
      head appendages number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

      prothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs sometimes partiallysize decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031934 RA
        MTQTQQRYA QIEKELLVI CFGVNKFYQ 
        YVFGKKFNV ETDHKPLIS IFKKPLNDC 
        PARLQRMLL SLQKFDINL IYKPGKKLI 
        IADHLSRSN LNSEDEIVD ISDVLEQNG 
        DLNHLEHSA EIASDENDS ASGASDENN 
        SASEILDCN TNNTGNIET ENKQKDCSA 
        VKYTRSGRR ISKPSRFKD VGK
      CDS sequence
      • >TC031934 RA
        atgacacaa acacaacag cggtacgct caaattgaa 
        aaagagcta ttggtcatc tgttttggg gtaaacaaa 
        ttttatcag tacgtgttc gggaaaaaa tttaacgta 
        gaaactgat cacaaacca ttaatctca atttttaag 
        aaaccttta aatgattgt ccagcccgg ttacaaaga 
        atgctttta tcattacaa aaattcgac ataaactta 
        atttataag cccgggaaa aaattaata attgcagat 
        cacttatca agatctaat ttaaattct gaggacgaa 
        attgtagat ataagtgat gtattagaa caaaacggt 
        gacttaaat catttggaa catagtgcg gaaattgca 
        agtgatgaa aatgacagt gcatctggt gctagtgac 
        gaaaataat agtgcaagt gaaattttg gactgtaac 
        acgaacaat accggaaat atcgaaacc gaaaataaa 
        caaaaagac tgtagtgcg gtaaagtac accaggtcc 
        ggtcgtaga ataagtaaa ccaagtaga tttaaggat 
        gttggaaaa taa
      mRNA sequence
      • >TC031934 RA
        cacgcggtc aaacaatta acgcaagtg ttacgttgt 
        taatacgta tgcaaggac acagtcaca aggaaatgc 
        accggaaat agatgcaat aaacaagaa acgagcacc 
        ggcgagatc aaagaagta cgaaaatcc caggaacga 
        gaggcacta cgaccactt ccaagaacg gaacccgaa 
        caaacaagg taataaact cataaaggg atgttcaag 
        atgtggacg tgaccatcc aatgaataa ttgtccggc 
        ctacgggaa aatttgtaa tttatgcaa aaaaccaaa 
        tcattttgc aaatttttg taaatttaa caattcaaa 
        aagaaaagt caatgaaat tgaatattg taatgacgc 
        agcaagcac ttcagctga ttattttgt aattgaaag 
        cattgactc aattgaaaa taacataag taatcctag 
        tttaataga cttagaaat taataataa aattataag 
        gtgtaaaat agatagcgg tgcaagcgc taatgtaat 
        ttcgaaaac gacactaat aaatttaga ttttgattt 
        aagaaaaat acaaaaaac aaatgacaa attagttac 
        gtttacagg gcaaaacat tccgcttct cggaaaatg 
        ttatttaaa gataaaatt taataacaa aagttatga 
        gacatgttt ttatgtaga aaaaggctc gcatgaacc 
        aataattgg tattcacac aagtgtttc gattggact 
        tttaacaat aaatgaaga aattaaggt aatagataa 
        aaagtcaaa taattatgt tcaattaac aaacaagta 
        caaagacat tttttttgg gtatcggta aattaagta 
        aaccgtatc atataaata ttcagaaag acacaaaac 
        caatcgtca acccaatta gaagtgttc catttgcat 
        taagggatg aatttaagc aatgtttag aggaattac 
        aaagagcga atatcatta aaagagtgg aaggtagca 
        cagaatggc taaattcat atgttttag ttaaaaaag 
        ctgacgggt cattaagaa tatgtttgg acccaaaat 
        atttaaatc aagcaatca taaatcaaa gttacaagc 
        taccaagta cagacgaaa taatttcaa aactaaaag 
        actcaaaat tcttttcca cattagacg cggcaaacg 
        gtttttgga atattcctc tcgatgacg aatcaagta 
        aattatgta catttggca cacctttcg gtaggttta 
        gatttttaa gaatgccat ttggaataa aaatcgcaa 
        gcgaggttt ttcaggaat atttctacg acatatttt 
        cgatggaag gcgttgaaa tttatattg acgatattt 
        taatacacg ccaaaacaa aagaagagc acgatatta 
        aattagaaa aagtttttc agttagcgc gtaagcata 
        atataaaat ttaatgcaa aaaaatgtc tactcggtg 
        ctaatgaag ttaaatatt taggttaca aattttcag 
        acaaaggcg tatcaattg atgaagaaa aattagagg 
        ccataaaaa acatgcctt ctcctacaa ataaaaaag 
        aagtgcaaa gatttttgg gtttaataa cgtatgtag 
        atcgattca taaaaaatc tttcggaaa aaactcatc 
        cactacgtg aaataatta aacgagaaa atatttttt 
        attgggggg aagaacaac aaaaaacat ttgatgaat 
        taaaaaatc ttttagcta ataaaccga ttttacaat 
        actttgacg caaataaag aaatcactt tgtcggtag 
        atgcttcgc aaaaaggtt tgggtgcag ttttattgc 
        aggataata aaccatgtg cctatgctt caagggcaa 
        tgacacaaa cacaacagc ggtacgctc aaattgaaa 
        aagagctat tggtcatct gttttgggg taaacaaat 
        tttatcagt acgtgttcg ggaaaaaat ttaacgtag 
        aaactgatc acaaaccat taatctcaa tttttaaga 
        aacctttaa atgattgtc cagcccggt tacaaagaa 
        tgcttttat cattacaaa aattcgaca taaacttaa 
        tttataagc ccgggaaaa aattaataa ttgcagatc 
        acttatcaa gatctaatt taaattctg aggacgaaa 
        ttgtagata taagtgatg tattagaac aaaacggtg 
        acttaaatc atttggaac atagtgcgg aaattgcaa 
        gtgatgaaa atgacagtg catctggtg ctagtgacg 
        aaaataata gtgcaagtg aaattttgg actgtaaca 
        cgaacaata ccggaaata tcgaaaccg aaaataaac 
        aaaaagact gtagtgcgg taaagtaca ccaggtccg 
        gtcgtagaa taagtaaac caagtagat ttaaggatg 
        ttggaaaat aaactaatt agtacatac gtttgttaa 
        ctgttaaag aaataataa cataaaaaa ggagatttt 
        gcatgatac gaatttaga tgttatgaa atgctagtg 
        tggtaatgt taatagata agtttgtta ccatttcct 
        tattttgtt ttataagga aaggagatg tcatgatat 
        acatgtatg tttggttgc ataacttgt agatattgt 
        aaatattgg aactttaaa ataaaatca gtcttcaac 
        t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY