Status
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Gene information
General information
TC031931
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004108
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452207.1: 36693..38787
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031931
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031931 RA
      MSVGFGQRG GNRPPPNPA QVQKMLDEN GHLIQTIQE 
      YQSKGKAPE VVQYQQALH RNLTYLATM ADSAQNVQS 
      FLPMLQTQA PPPPHGQPP PTMDQQHMN NFNHQQQPA 
      YRQPPGPQR PAFQPTLTP SEVTNKTNT LANTHLKVT 
      PIPNKTNTP QTNPRSIPH RMLKPITDN HRQRQHTTT 
      TNSRVTARP HLVEPHPNR VQGPTLRQV PRPKPHPGR 
      TLMVAPTPI PRLHRLTHR DTRPSRATP RLHPLANHK 
      RPPPLIPTN QVRLHKITG LVIPSLRPF RLHPIIIKV 
      LRLLRLHPP HHNRINLHI HPILSPRIL TQDTHPIPH 
      KGIPLQDRP IIMGMPHNL AIHHSLNMG DNHKGTSIQ 
      GLQQHKVHH LRKGSMGTI TLPSQTHSR KSLFYVPFF 
      RNQLEVLIV FLRFF
    • >TC031931 RB
      MLQTQAPPP PHGQPPPTM DQQHMNNFN HQQQPAYRQ 
      PPGPQRPAF QPTLTPSEV TNKTNTLAN THLKVTPIP 
      NKTNTPQTN PRSIPHRML KPITDNHRQ RQHTTTTNS 
      RVTARPHLV EPHPNRVQG PTLRQVPRP KPHPGRTLM 
      VAPTPIPRL HRLTHRDTR PSRATPRLH PLANHKRPP 
      PLIPTNQVR LHKITGLVI PSLRPFRLH PIIIKVLRL 
      LRLHPPHHN RINLHIHPI LSPRILTQD THPIPHKGI 
      PLQDRPIIM GMPHNLAIH HSLNMGDNH KGTSIQGLQ 
      QHKVHHLRK GSMGTITLP SQTHSRKSL FYVPFFRNQ 
      LEVLIVFLR FF
    CDS sequence
    • >TC031931 RA
      atgtctgtg ggattcggt caacgtggc ggtaatcga 
      ccgccacca aacccagca caagtacaa aaaatgctc 
      gacgaaaat ggacatttg attcaaaca attcaagag 
      tatcaaagc aaaggaaaa gctccagaa gttgtccag 
      tatcaacaa gcattgcac aggaattta acttatcta 
      gccacgatg gcagactct gctcaaaat gttcagtct 
      tttttgcct atgttgcaa acacaggca cccccacca 
      ccgcacggc cagccgcca cccacaatg gatcaacag 
      cacatgaac aatttcaac caccaacaa caacccgcc 
      taccgccag cctccagga ccgcaacgc cctgcgttc 
      caacccacc cttaccccc agcgaggtt accaacaaa 
      accaatacc ctggccaat acccacctc aaggtaacg 
      cctatcccc aacaaaacc aatacccca caaaccaac 
      ccccggtct atcccccat cgaatgctc aagccaatt 
      acggacaac caccgtcaa cgacagcac acaacaact 
      accaacagc cgggttacg gcacgcccc cacctggtg 
      gagccccac cccaaccgg gtgcagggc cctaccctc 
      cgccaggtg ccccgcccc aagccccac ccgggacgt 
      accctaatg gtggcccct accccaatt ccacggctc 
      cacagactt acccacagg gatacccgc cccagccgg 
      gctacgccc cgcctccac cctctagcc aaccacaaa 
      cgtcccccg cctcttatc ccaaccaac caagtacgt 
      ctccacaaa attacgggc ctagtaata cccagtctc 
      cgccctttt cggctccac ccaataata atcaaagtc 
      ctgcggtta cttcggctc cacccacca caccacaac 
      cgtatcaac ctccatatc cacccaatt ctcagccct 
      cgaatccta acgcaggat acacaccca ataccccac 
      aagggtatc cccctgcag gaccgccca ataattatg 
      ggtatgccc cacaacctg gctatccac cacagcctc 
      aatatgggg gacaaccac aagggtacc agtatccaa 
      ggcctccaa cagcacaag gtccaccac ctccgcaag 
      ggcagtatg ggtacaatt accctgccc agccaaacc 
      cacagtaga aagtcctta ttctatgtt ccatttttt 
      aggaatcaa ctcgaagta cttatcgta tttctgcgt 
      tttttttag
    • >TC031931 RB
      atgttgcaa acacaggca cccccacca ccgcacggc 
      cagccgcca cccacaatg gatcaacag cacatgaac 
      aatttcaac caccaacaa caacccgcc taccgccag 
      cctccagga ccgcaacgc cctgcgttc caacccacc 
      cttaccccc agcgaggtt accaacaaa accaatacc 
      ctggccaat acccacctc aaggtaacg cctatcccc 
      aacaaaacc aatacccca caaaccaac ccccggtct 
      atcccccat cgaatgctc aagccaatt acggacaac 
      caccgtcaa cgacagcac acaacaact accaacagc 
      cgggttacg gcacgcccc cacctggtg gagccccac 
      cccaaccgg gtgcagggc cctaccctc cgccaggtg 
      ccccgcccc aagccccac ccgggacgt accctaatg 
      gtggcccct accccaatt ccacggctc cacagactt 
      acccacagg gatacccgc cccagccgg gctacgccc 
      cgcctccac cctctagcc aaccacaaa cgtcccccg 
      cctcttatc ccaaccaac caagtacgt ctccacaaa 
      attacgggc ctagtaata cccagtctc cgccctttt 
      cggctccac ccaataata atcaaagtc ctgcggtta 
      cttcggctc cacccacca caccacaac cgtatcaac 
      ctccatatc cacccaatt ctcagccct cgaatccta 
      acgcaggat acacaccca ataccccac aagggtatc 
      cccctgcag gaccgccca ataattatg ggtatgccc 
      cacaacctg gctatccac cacagcctc aatatgggg 
      gacaaccac aagggtacc agtatccaa ggcctccaa 
      cagcacaag gtccaccac ctccgcaag ggcagtatg 
      ggtacaatt accctgccc agccaaacc cacagtaga 
      aagtcctta ttctatgtt ccatttttt aggaatcaa 
      ctcgaagta cttatcgta tttctgcgt tttttttag
    mRNA sequence
    • >TC031931 RA
      tagaaaaaa caccagtca agtgaactc gaacgaaat 
      tacgacaaa tgtgagctt cgtttttcg ttaaaataa 
      ttgttttta agtgcaacc acgttaatt aagtgaaaa 
      tgtctgtgg gattcggtc aacgtggcg gtaatcgac 
      cgccaccaa acccagcac aagtacaaa aaatgctcg 
      acgaaaatg gacatttga ttcaaacaa ttcaagagt 
      atcaaagca aaggaaaag ctccagaag ttgtccagt 
      atcaacaag cattgcaca ggaatttaa cttatctag 
      ccacgatgg cagactctg ctcaaaatg ttcagtctt 
      ttttgccta tgttgcaaa cacaggcac ccccaccac 
      cgcacggcc agccgccac ccacaatgg atcaacagc 
      acatgaaca atttcaacc accaacaac aacccgcct 
      accgccagc ctccaggac cgcaacgcc ctgcgttcc 
      aacccaccc ttaccccca gcgaggtta ccaacaaaa 
      ccaataccc tggccaata cccacctca aggtaacgc 
      ctatcccca acaaaacca ataccccac aaaccaacc 
      cccggtcta tcccccatc gaatgctca agccaatta 
      cggacaacc accgtcaac gacagcaca caacaacta 
      ccaacagcc gggttacgg cacgccccc acctggtgg 
      agccccacc ccaaccggg tgcagggcc ctaccctcc 
      gccaggtgc cccgcccca agccccacc cgggacgta 
      ccctaatgg tggccccta ccccaattc cacggctcc 
      acagactta cccacaggg atacccgcc ccagccggg 
      ctacgcccc gcctccacc ctctagcca accacaaac 
      gtcccccgc ctcttatcc caaccaacc aagtacgtc 
      tccacaaaa ttacgggcc tagtaatac ccagtctcc 
      gcccttttc ggctccacc caataataa tcaaagtcc 
      tgcggttac ttcggctcc acccaccac accacaacc 
      gtatcaacc tccatatcc acccaattc tcagccctc 
      gaatcctaa cgcaggata cacacccaa taccccaca 
      agggtatcc ccctgcagg accgcccaa taattatgg 
      gtatgcccc acaacctgg ctatccacc acagcctca 
      atatggggg acaaccaca agggtacca gtatccaag 
      gcctccaac agcacaagg tccaccacc tccgcaagg 
      gcagtatgg gtacaatta ccctgccca gccaaaccc 
      acagtagaa agtccttat tctatgttc catttttta 
      ggaatcaac tcgaagtac ttatcgtat ttctgcgtt 
      ttttttagt ttttttctc tcgagaaat agtgtgata 
      ttgaatgtt ttttctttt gtatcgcca tttcattgt 
      aataaattt gacaaaata agcacttaa tcattaatg 
      accacagcc ctccaaacg ccaaatttc ttcaaaaat 
      ataaaataa aaacgtaac tagaac
    • >TC031931 RB
      tagaaaaaa caccagtca agtgaactc gaacgaaat 
      tacgacaaa tgtgagctt cgtttttcg ttaaaataa 
      ttgttttta agtgcaacc acgttaatt aagtgaaaa 
      tgtctgtgg gattcggtc aacgtggcg gtaatcgac 
      cgccaccaa acccagcac aagtacaaa aaatgctcg 
      acgaaaatg gacatttga ttcaaacaa ttcaagagt 
      atcaaagca aaggaaaag ctccagaag ttgtccagt 
      atcaacaag cattgcaca ggaatttaa cttatctag 
      ccacgatgg cagactctg ctcaaaatg ttcagtctt 
      ttttgccta tgttgcaaa cacaggcac ccccaccac 
      cgcacggcc agccgccac ccacaatgg atcaacagc 
      acatgaaca atttcaacc accaacaac aacccgcct 
      accgccagc ctccaggac cgcaacgcc ctgcgttcc 
      aacccaccc ttaccccca gcgaggtta ccaacaaaa 
      ccaataccc tggccaata cccacctca aggtaacgc 
      ctatcccca acaaaacca ataccccac aaaccaacc 
      cccggtcta tcccccatc gaatgctca agccaatta 
      cggacaacc accgtcaac gacagcaca caacaacta 
      ccaacagcc gggttacgg cacgccccc acctggtgg 
      agccccacc ccaaccggg tgcagggcc ctaccctcc 
      gccaggtgc cccgcccca agccccacc cgggacgta 
      ccctaatgg tggccccta ccccaattc cacggctcc 
      acagactta cccacaggg atacccgcc ccagccggg 
      ctacgcccc gcctccacc ctctagcca accacaaac 
      gtcccccgc ctcttatcc caaccaacc aagtacgtc 
      tccacaaaa ttacgggcc tagtaatac ccagtctcc 
      gcccttttc ggctccacc caataataa tcaaagtcc 
      tgcggttac ttcggctcc acccaccac accacaacc 
      gtatcaacc tccatatcc acccaattc tcagccctc 
      gaatcctaa cgcaggata cacacccaa taccccaca 
      agggtatcc ccctgcagg accgcccaa taattatgg 
      gtatgcccc acaacctgg ctatccacc acagcctca 
      atatggggg acaaccaca agggtacca gtatccaag 
      gcctccaac agcacaagg tccaccacc tccgcaagg 
      gcagtatgg gtacaatta ccctgccca gccaaaccc 
      acagtagaa agtccttat tctatgttc catttttta 
      ggaatcaac tcgaagtac ttatcgtat ttctgcgtt 
      ttttttagt ttttttctc tcgagaaat agtgtgata 
      ttgaatgtt ttttctttt gtatcgcca tttcattgt 
      aataaattt gacaaaata agcacttaa tcattaatg 
      accacagcc ctccaaacg ccaaatttc ttcaaaaat 
      ataaaataa aaacgtaac tagaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY