Status
Please wait ...
Gene information
General information
TC001392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452119.1: 3463..5043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001392
    iBeetle screen iB_09576

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 2

    tropharium structure irregular - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001392 RA
      MTTSEQIGL NKTWELSLY ELHRTPQDV ITDNTEIAV 
      SPRSLHSEL MCPICLDML KKTMTTKEC LHRFCSDCI 
      ITALRSGNK ECPTCRKKL VSKRSLRPD PNFDLLISK 
      IYPSRDEYE AHQERVLAK LNKSHSQAA LVNSITEGI 
      KIQSQNRPN RSRKNNEVE RNQGENPEN PVPPNENTV 
      GAPQPAPGV VNNGSPAPS GRSTPSQPP SPVSSNVSS 
      ISKSIKRPK STLTSERSE ESESSERTE QTDTEGEGP 
      SEPMTLNEI ELVFKPHPT EMSGDNPLV KALKENSIR 
      YIKTTANAT VDHLHKYLA MRLTLDLDS QLPTTHSLL 
      NFCIYISPT AGQYVQLNG NQTLGQVND KYWKVNKPL 
      EMYYSWKKT
    CDS sequence
    • >TC001392 RA
      atgacgacg tccgagcaa atcggacta aacaaaaca 
      tgggaattg tccctttac gagctccac cgcaccccc 
      caggacgtc ataaccgac aacaccgag atcgccgtg 
      tccccccgc agcctccac agcgagctc atgtgcccc 
      atctgcctc gacatgctc aagaagacc atgacgacg 
      aaagaatgc ctccaccgc ttctgctcc gactgcata 
      atcacagcc ctgcgctca gggaacaag gagtgcccc 
      acctgtcgc aagaaactc gtctcgaag cgctcgctg 
      cgccccgac cccaacttc gacctcctc atctcgaaa 
      atctacccc agtcgcgac gaatacgaa gcccaccag 
      gagcgagtc ctggccaag ctgaacaaa agccactca 
      caggccgct ttagtcaac tcaatcacc gaaggcatc 
      aaaatccag tcacaaaac cgccccaac cgctcgcgc 
      aaaaacaac gaagttgag cgaaatcaa ggggaaaac 
      cccgaaaat cccgtgcct ccgaatgaa aacacagtc 
      ggggccccg cagcccgcg cccggcgtc gtgaacaac 
      gggagcccc gcgccgtcg gggcgcagc accccgtcg 
      cagccgcct agtcccgtc tcgtccaac gtcagctcg 
      atcagcaag tcgataaag cgccccaaa agcacgctc 
      acgtccgaa cgaagcgag gagagcgag tcgagtgag 
      cgcaccgag cagacggac acggagggc gagggcccc 
      tcggaaccc atgactttg aatgagatc gagctcgtt 
      tttaagcca cacccgacg gaaatgtcc ggtgataac 
      ccccttgtt aaggctttg aaggagaac tcaatcaga 
      tatattaag acaacggca aatgccact gttgaccat 
      ttgcataag tatctagcg atgaggcta acgctcgat 
      ttggattcg caattaccg acgacgcat agtttgctt 
      aatttttgt atttatatc tcgccaacg gctggacag 
      tacgtgcag ttgaacggg aaccagact ttggggcaa 
      gtcaacgac aagtactgg aaggtgaat aagcccttg 
      gagatgtac tactcgtgg aagaagaca tag
    mRNA sequence
    • >TC001392 RA
      acaaaatta ggggcaaag cagtgccaa ccctcacct 
      cacctgtgc catcactta tctctggtc acaatcgca 
      ttattaaga caattgtga gaactttgg ttgtaagac 
      tttggtttt aaattgcct aacctataa atcaattgt 
      caaaaaaac ttgcgattt ttgtaccgc atggttggt 
      gctcccgat cacacaatt aattctttt atatcgcaa 
      tttgtgggc attattaca attagagga cccaaatat 
      tactaaaac gcattaaat aatgtaaat atgatgcgt 
      gataatgat ttaatttcg tggtttcgc tattagtgc 
      gtgttccat ggttagttc ccgttccgc ggttggcaa 
      ttcgcttgt tgccatttc tccctagtg tcaaaatga 
      cgacgtccg agcaaatcg gactaaaca aaacatggg 
      aattgtccc tttacgagc tccaccgca ccccccagg 
      acgtcataa ccgacaaca ccgagatcg ccgtgtccc 
      cccgcagcc tccacagcg agctcatgt gccccatct 
      gcctcgaca tgctcaaga agaccatga cgacgaaag 
      aatgcctcc accgcttct gctccgact gcataatca 
      cagccctgc gctcaggga acaaggagt gccccacct 
      gtcgcaaga aactcgtct cgaagcgct cgctgcgcc 
      ccgacccca acttcgacc tcctcatct cgaaaatct 
      accccagtc gcgacgaat acgaagccc accaggagc 
      gagtcctgg ccaagctga acaaaagcc actcacagg 
      ccgctttag tcaactcaa tcaccgaag gcatcaaaa 
      tccagtcac aaaaccgcc ccaaccgct cgcgcaaaa 
      acaacgaag ttgagcgaa atcaagggg aaaaccccg 
      aaaatcccg tgcctccga atgaaaaca cagtcgggg 
      ccccgcagc ccgcgcccg gcgtcgtga acaacggga 
      gccccgcgc cgtcggggc gcagcaccc cgtcgcagc 
      cgcctagtc ccgtctcgt ccaacgtca gctcgatca 
      gcaagtcga taaagcgcc ccaaaagca cgctcacgt 
      ccgaacgaa gcgaggaga gcgagtcga gtgagcgca 
      ccgagcaga cggacacgg agggcgagg gcccctcgg 
      aacccatga ctttgaatg agatcgagc tcgttttta 
      agccacacc cgacggaaa tgtccggtg ataaccccc 
      ttgttaagg ctttgaagg agaactcaa tcagatata 
      ttaagacaa cggcaaatg ccactgttg accatttgc 
      ataagtatc tagcgatga ggctaacgc tcgatttgg 
      attcgcaat taccgacga cgcatagtt tgcttaatt 
      tttgtattt atatctcgc caacggctg gacagtacg 
      tgcagttga acgggaacc agactttgg ggcaagtca 
      acgacaagt actggaagg tgaataagc ccttggaga 
      tgtactact cgtggaaga agacatagg gaacgcgga 
      aggggggca ccaaggccc cctaataag gcatttgta 
      cggtgtaat atttaataa aaaatattt aatgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY