Status
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Gene information
General information
TC031906
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016006
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450959.1: 8611..13533
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031906
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031906 RA
      MEVVSYFFS LFLDFLIVF LVSIQASPA WQRNNHIVF 
      PNSLEEHET FIAPKCSGN KTFCEDVGH YPKNKFSVI 
      LENTTYGED YFMPQSTAE EESIKNRLY GVTEYYICN 
      SHVQTQYPK VAQNVRNEW KYVYNFDNY KQGVRTEEC 
      VVRCTCSED STQPSVAVE EMWVFVLVF AVFKELGIQ 
      AMSFNPAKM DDRKGLTFP DSSEIGGDF LYETVVPNC 
      QNHTYCEHL DRYPNELFS KILKSREKE FSRYFQSMA 
      VIEPIMSRN QDTSCICAS RKSIIYPKA AYNIKNNLK 
      FIYNFDGYK QGVSVEVCV IKNQMCKFY EKVRKLKTR 
      CRQKFVEKL LLTADENGR PYADSWVTA E
    • >TC031906 RB
      MPQSTAEEE SIKNRLYGV TEYYICNSH VQTQYPKVA 
      QNVRNEWKY VYNFDNYKQ GVRTEECVV RCTCSEDST 
      QPSVAVEEM WVFVLVFAV FKELGIQAM SFNPAKMVL 
      DDRKGLTFP DSSEIGGDF LYETVVPNC QNHTYCEHL 
      DRYPNELFS KILKSREKE FSRYFQSMA VIEPIMSRN 
      QDTSCICAS RKSIIYPKA AYNIKNNLK FIYNFDGYK 
      QGVSVEVCV IKNQMCKFY EKVRKLKTR CRQKFVEKL 
      LLTADENGR PYADSWVTA E
    • >TC031906 RC
      MPQSTAEEE SIKNRLYGV TEYYICNSH VQTQYPKVA 
      QNVRNEWKY VYNFDNYKQ GVRTEECVV RCTCSEDST 
      QPSVAVEEM WVFVLVFAV FKELGIQAM SFNPAKMDD 
      RKGLTFPDS SEIGGDFLY ETVVPNCQN HTYCEHLDR 
      YPNELFSKI LKSREKEFS RYFQSMAVI EPIMSRNQD 
      TSCICASRK SIIYPKAAY NIKNNLKFI YNFDGYKQG 
      VSVEVCVIK NQMCKFYEK VRKLKTRCR QKFVEKLLL 
      TADENGRPY ADSWVTAE
    CDS sequence
    • >TC031906 RA
      atggaagtg gttagttat ttcttttct ctttttctg 
      gattttctt atagttttt ttagtcagt attcaagca 
      agtccagcc tggcagaga aacaatcac atcgttttt 
      ccgaactcg ctagaagag catgaaact ttcattgct 
      ccaaagtgt agcggtaac aaaacattt tgtgaggac 
      gtcggacat taccccaaa aataaattt agcgtaata 
      ttagaaaac accacatac ggggaagat tatttcatg 
      ccgcaaagc accgccgag gaagaatct atcaaaaat 
      cgattgtac ggagtaact gaatattac atctgcaat 
      tctcacgtc caaacgcaa tatcccaaa gtggcgcaa 
      aacgtcaga aacgagtgg aaatatgtt tacaatttt 
      gataactac aagcaaggc gtaaggaca gaagaatgt 
      gttgtcaga tgtacctgt tcagaagac agtacacaa 
      ccttcagtg gcagtggaa gaaatgtgg gtttttgtg 
      ttggttttt gccgttttc aaagagttg ggcattcaa 
      gccatgtca tttaatcca gccaagatg gatgatcgc 
      aagggtttg acctttccg gattcgagt gaaattggg 
      ggtgatttt ttatacgag acggtggtc cccaactgc 
      caaaatcac acgtactgt gaacacttg gaccgctac 
      cctaacgaa ctattttcc aaaatatta aaaagccgc 
      gaaaaggag ttttctcgg tactttcag agtatggca 
      gtaatagag cccattatg agtaggaac caagacact 
      tcctgtatt tgtgcatca cgcaaaagt attatatac 
      ccaaaagcg gcatataat ataaaaaat aatctgaaa 
      tttatctac aacttcgat ggatacaaa caaggagtg 
      tccgtagag gtctgcgtg ataaaaaat caaatgtgc 
      aaattttac gagaaggtg agaaagtta aaaacaagg 
      tgtcgacaa aagttcgtc gaaaaattg ttactgacg 
      gctgatgaa aatggaaga ccgtacgct gatagttgg 
      gtaactgca gagtaa
    • >TC031906 RB
      atgccgcaa agcaccgcc gaggaagaa tctatcaaa 
      aatcgattg tacggagta actgaatat tacatctgc 
      aattctcac gtccaaacg caatatccc aaagtggcg 
      caaaacgtc agaaacgag tggaaatat gtttacaat 
      tttgataac tacaagcaa ggcgtaagg acagaagaa 
      tgtgttgtc agatgtacc tgttcagaa gacagtaca 
      caaccttca gtggcagtg gaagaaatg tgggttttt 
      gtgttggtt tttgccgtt ttcaaagag ttgggcatt 
      caagccatg tcatttaat ccagccaag atggtttta 
      gatgatcgc aagggtttg acctttccg gattcgagt 
      gaaattggg ggtgatttt ttatacgag acggtggtc 
      cccaactgc caaaatcac acgtactgt gaacacttg 
      gaccgctac cctaacgaa ctattttcc aaaatatta 
      aaaagccgc gaaaaggag ttttctcgg tactttcag 
      agtatggca gtaatagag cccattatg agtaggaac 
      caagacact tcctgtatt tgtgcatca cgcaaaagt 
      attatatac ccaaaagcg gcatataat ataaaaaat 
      aatctgaaa tttatctac aacttcgat ggatacaaa 
      caaggagtg tccgtagag gtctgcgtg ataaaaaat 
      caaatgtgc aaattttac gagaaggtg agaaagtta 
      aaaacaagg tgtcgacaa aagttcgtc gaaaaattg 
      ttactgacg gctgatgaa aatggaaga ccgtacgct 
      gatagttgg gtaactgca gagtaa
    • >TC031906 RC
      atgccgcaa agcaccgcc gaggaagaa tctatcaaa 
      aatcgattg tacggagta actgaatat tacatctgc 
      aattctcac gtccaaacg caatatccc aaagtggcg 
      caaaacgtc agaaacgag tggaaatat gtttacaat 
      tttgataac tacaagcaa ggcgtaagg acagaagaa 
      tgtgttgtc agatgtacc tgttcagaa gacagtaca 
      caaccttca gtggcagtg gaagaaatg tgggttttt 
      gtgttggtt tttgccgtt ttcaaagag ttgggcatt 
      caagccatg tcatttaat ccagccaag atggatgat 
      cgcaagggt ttgaccttt ccggattcg agtgaaatt 
      gggggtgat tttttatac gagacggtg gtccccaac 
      tgccaaaat cacacgtac tgtgaacac ttggaccgc 
      taccctaac gaactattt tccaaaata ttaaaaagc 
      cgcgaaaag gagttttct cggtacttt cagagtatg 
      gcagtaata gagcccatt atgagtagg aaccaagac 
      acttcctgt atttgtgca tcacgcaaa agtattata 
      tacccaaaa gcggcatat aatataaaa aataatctg 
      aaatttatc tacaacttc gatggatac aaacaagga 
      gtgtccgta gaggtctgc gtgataaaa aatcaaatg 
      tgcaaattt tacgagaag gtgagaaag ttaaaaaca 
      aggtgtcga caaaagttc gtcgaaaaa ttgttactg 
      acggctgat gaaaatgga agaccgtac gctgatagt 
      tgggtaact gcagagtaa
    mRNA sequence
    • >TC031906 RA
      atggcataa attcagaga gtggttttg ggaacagga 
      aacaaacac gaaaaaatg aaattttga ttgaaacgc 
      cactgtttg tttttgttt atgtcttta cataaaaca 
      cgtaacctt gacctatcg tccacactt aaacaaaaa 
      actaattgg tcgctttca ggggatttg ctgggcggg 
      gagttcggc gaccaggac ataatattt cccgacacg 
      tactaagtg attttcaat gatgttccg aaaatcgac 
      ggtcctcct aagtgtgcc gaagacaac actttttgt 
      gaggacttt gattcttat ccttacaac cacgtgaaa 
      aacgtgctt aagcgcaag cgggtgcat actgattta 
      ttcggcaag gacgaaccc ctcgatgag cgctacagc 
      atcaccaac agaaacggc gacttcgag gcgttcatc 
      tgcacgagc atatccaaa acgattttc ccccgagtt 
      ggcatcaac aaaaacaac aagtggaag gtcataatc 
      aatcaagag gaagacgga tacattcaa gggattagg 
      acggaaatt tgccgaaag taaagggtt gaaagttga 
      tttctgtgt cggtgacag agtgttgcg gggaagggg 
      cggcttgtg acctcatcg gcgacaggg cgaacggct 
      acataacca gctgcaagc aaaaataca tgttgcgga 
      ggatgatgt ctttatctg aaaatgggg atcccgtcc 
      cggacacat tccagttac cgtcagcct gctgctgct 
      cttacagga aggatctga gttttttgt cccgatttg 
      gcgccgacg atgtttcct taaggtcga aagggaaag 
      gaaaataaa ataaagaat gtgatataa gttatgtga 
      ttgaacttt ttattgtaa aaacggcag cgggattat 
      ttattgaat aaaatcata cagaagcaa accacaacc 
      aatgaacga acccttctc cccacctcc ggccgaaac 
      cctaaaccc tttcaactt tcctaatca cttatttac 
      gattctatt caacattat tcttcgtgg ctgcaaacg 
      tcgaatgtg ggtgaagtg tgttttcgt ctgtgttta 
      ttttatgac tatggaagt ggttagtta tttcttttc 
      tctttttct ggattttct tatagtttt tttagtcag 
      tattcaagc aagtccagc ctggcagag aaacaatca 
      catcgtttt tccgaactc gctagaaga gcatgaaac 
      tttcattgc tccaaagtg tagcggtaa caaaacatt 
      ttgtgagga cgtcggaca ttaccccaa aaataaatt 
      tagcgtaat attagaaaa caccacata cggggaaga 
      ttatttcat gccgcaaag caccgccga ggaagaatc 
      tatcaaaaa tcgattgta cggagtaac tgaatatta 
      catctgcaa ttctcacgt ccaaacgca atatcccaa 
      agtggcgca aaacgtcag aaacgagtg gaaatatgt 
      ttacaattt tgataacta caagcaagg cgtaaggac 
      agaagaatg tgttgtcag atgtacctg ttcagaaga 
      cagtacaca accttcagt ggcagtgga agaaatgtg 
      ggtttttgt gttggtttt tgccgtttt caaagagtt 
      gggcattca agccatgtc atttaatcc agccaagat 
      ggatgatcg caagggttt gacctttcc ggattcgag 
      tgaaattgg gggtgattt tttatacga gacggtggt 
      ccccaactg ccaaaatca cacgtactg tgaacactt 
      ggaccgcta ccctaacga actattttc caaaatatt 
      aaaaagccg cgaaaagga gttttctcg gtactttca 
      gagtatggc agtaataga gcccattat gagtaggaa 
      ccaagacac ttcctgtat ttgtgcatc acgcaaaag 
      tattatata cccaaaagc ggcatataa tataaaaaa 
      taatctgaa atttatcta caacttcga tggatacaa 
      acaaggagt gtccgtaga ggtctgcgt gataaaaaa 
      tcaaatgtg caaatttta cgagaaggt gagaaagtt 
      aaaaacaag gtgtcgaca aaagttcgt cgaaaaatt 
      gttactgac ggctgatga aaatggaag accgtacgc 
      tgatagttg ggtaactgc agagtaata catttactt 
      tgtggagaa gtgaactga gacgatttt atccctgta 
      attatcgca gtattaggt ggaagaaaa cgataggta 
      ttaatcaca atttagtga atcccagct aataattct 
      tgaaaagta attacgtgt gatttgtgt gattatgac 
      gaattgtag gtacgtaag ttattgtgg actaatgtg 
      tgtttttat aattaaatt ttagcaatt ttagtccgg 
      acgttagtc aagttttta tttattgtt acaagcttt 
      accagtgtc tgaaactgt ttaagcatt ttatgaaat 
      attatgtta gaactagct taagcatta aaaatgtac 
      aaacagacg aaaagtatt gtagtattg taaatttat 
      gtgtactat aaactgtaa ttaataaag ctgttgatg 
      ttcgaatga acttatttt atgtgggaa aataaataa 
      aatttgttg caaacagga aagctgtta ataactgaa 
      acttaaatg aatttcctt cagttttgt ttgaggaac 
      ggtgtggtc tgatgtgcg tccgtgatg ctgagttat 
      ttgtatttg aaataagaa gcctctaat ttaatagta 
      ataggtgag ttggttaaa ttcaaccaa aacgtagtg 
      tgggctctt cttgcacct ttattgtaa caaaaaaat 
      tccacatat ctcactctt aatacttta attaattgc 
      tgttgtgtt ttgaggggg gatttattg gttttatga 
      atttattta aatagttta aaaaaaatt ttggcttgt 
      ggggctaag gatattcta atagaattt taaataatt 
      catatttgt ttagttttt tctggattg tttttgttt 
      tgaagaacg gtttttttt ctttttaga atttatttt 
      attgttgag ggattaact tgatttttg gaatttaat 
      ttgttaata attaactct agaagaatt gttatgact 
      tttttattt gactgaata tcatttctt tttatgaga 
      tttgtatta tttatttct agtatagta attttttat 
      aggcgtgaa tatattggt aggggctcc aaaatgtaa 
      ttttaacat tcggtcctt tcgtactag aacattaac 
      ataaataaa agatagaaa ccaacctgg ctcacgccg 
      gtttaaact cagatcatg taaaatttt aaaggtcga 
      acagaccca atattccag cttctacac cgaaaatta 
      attttaatc caacatcga ggtcgcaaa cttttctgt 
      aaatatgaa ctttcaaaa aaaattacg ctgttatcc 
      ctaaggtaa tttaatctt ttaatccaa aattcagga 
      tcaacaatt cactaatca gtgtaaaaa caaaaaaaa 
      agttaaatc aattttcct atcacccca acaaaattc 
      tttaaacta tcaaggaaa agaaaactt caataccaa 
      taaaaactt aaaaaataa aactctata gggtcttct 
      cgtcttttt aaaaaattt aagcattct cacttaaaa 
      ttcaaatta taatacaat taattagag acagttaat 
      ttctcatta aaccattca ttccagctt ctaattaag 
      aaactaatg attatgcta cctttgcac agtcaaaat 
      actgcggcc attaaaaca ctcattggg caggcccga 
      ccttaaatt ataatcaaa aagccatgt ttttaataa 
      acaggtgaa aattcaatt gccgagttt cttaaacta 
      accttataa ataaaaatt aattttaca ataaattat 
      actaatttt a
    • >TC031906 RB
      atggcataa attcagaga gtggttttg ggaacagga 
      aacaaacac gaaaaaatg aaattttga ttgaaacgc 
      cactgtttg tttttgttt atgtcttta cataaaaca 
      cgtaacctt gacctatcg tccacactt aaacaaaaa 
      actaattgg tcgctttca ggggatttg ctgggcggg 
      gagttcggc gaccaggac ataatattt cccgacacg 
      tactaagtg attttcaat gatgttccg aaaatcgac 
      ggtcctcct aagtgtgcc gaagacaac actttttgt 
      gaggacttt gattcttat ccttacaac cacgtgaaa 
      aacgtgctt aagcgcaag cgggtgcat actgattta 
      ttcggcaag gacgaaccc ctcgatgag cgctacagc 
      atcaccaac agaaacggc gacttcgag gcgttcatc 
      tgcacgagc atatccaaa acgattttc ccccgagtt 
      ggcatcaac aaaaacaac aagtggaag gtcataatc 
      aatcaagag gaagacgga tacattcaa gggattagg 
      acggaaatt tgccgaaag taaagggtt gaaagttga 
      tttctgtgt cggtgacag agtgttgcg gggaagggg 
      cggcttgtg acctcatcg gcgacaggg cgaacggct 
      acataacca gctgcaagc aaaaataca tgttgcgga 
      ggatgatgt ctttatctg aaaatgggg atcccgtcc 
      cggacacat tccagttac cgtcagcct gctgctgct 
      cttacagga aggatctga gttttttgt cccgatttg 
      gcgccgacg atgtttcct taaggtcga aagggaaag 
      gaaaataaa ataaagaat gtgatataa gttatgtga 
      ttgaacttt ttattgtaa aaacggcag cgggattat 
      ttattgaat aaaatcata cagaagcaa accacaacc 
      aatgaacga acccttctc cccacctcc ggccgaaac 
      cctaaaccc tttcaactt tcctaatca cttatttac 
      gattctatt caacattat tcttcgtgg ctgcaaacg 
      tcgaatgtg ggtgaagtg tgttttcgt ctgtgttta 
      ttttatgac tatggaagt ggttagtta tttcttttc 
      tctttttct ggattttct tatagtttt tttagtcag 
      tattcaagc aagtccagc ctggcagag taagattta 
      tcgccttga aaatgaaaa tttaattat tttttatgg 
      caggaaaca atcacatcg tttttccga actcgctag 
      aagagcatg aaactttca ttgctccaa agtgtagcg 
      gtaacaaaa cattttgtg aggacgtcg gacattacc 
      ccaaaaata aatttagcg taatattag aaaacacca 
      catacgggg aagattatt tcatgccgc aaagcaccg 
      ccgaggaag aatctatca aaaatcgat tgtacggag 
      taactgaat attacatct gcaattctc acgtccaaa 
      cgcaatatc ccaaagtgg cgcaaaacg tcagaaacg 
      agtggaaat atgtttaca attttgata actacaagc 
      aaggcgtaa ggacagaag aatgtgttg tcagatgta 
      cctgttcag aagacagta cacaacctt cagtggcag 
      tggaagaaa tgtgggttt ttgtgttgg tttttgccg 
      ttttcaaag agttgggca ttcaagcca tgtcattta 
      atccagcca agatggttt tagatgatc gcaagggtt 
      tgacctttc cggattcga gtgaaattg ggggtgatt 
      ttttatacg agacggtgg tccccaact gccaaaatc 
      acacgtact gtgaacact tggaccgct accctaacg 
      aactatttt ccaaaatat taaaaagcc gcgaaaagg 
      agttttctc ggtactttc agagtatgg cagtaatag 
      agcccatta tgagtagga accaagaca cttcctgta 
      tttgtgcat cacgcaaaa gtattatat acccaaaag 
      cggcatata atataaaaa ataatctga aatttatct 
      acaacttcg atggataca aacaaggag tgtccgtag 
      aggtctgcg tgataaaaa atcaaatgt gcaaatttt 
      acgagaagg tgagaaagt taaaaacaa ggtgtcgac 
      aaaagttcg tcgaaaaat tgttactga cggctgatg 
      aaaatggaa gaccgtacg ctgatagtt gggtaactg 
      cagagtaat acatttact ttgtggaga agtgaactg 
      agacgattt tatccctgt aattatcgc agtattagg 
      tggaagaaa acgataggt attaatcac aatttagtg 
      aatcccagc taataattc ttgaaaagt aattacgtg 
      tgatttgtg tgattatga cgaattgta ggtacgtaa 
      gttattgtg gactaatgt gtgttttta taattaaat 
      tttagcaat tttagtccg gacgttagt caagttttt 
      atttattgt tacaagctt taccagtgt ctgaaactg 
      tttaagcat tttatgaaa tattatgtt agaactagc 
      ttaagcatt aaaaatgta caaacagac gaaaagtat 
      tgtagtatt gtaaattta tgtgtacta taaactgta 
      attaataaa gctgttgat gttcgaatg aacttattt 
      tatgtggga aaataaata aaatttgtt gcaaacagg 
      aaagctgtt aataactga aacttaaat gaatttcct 
      tcagttttg tttgaggaa cggtgtggt ctgatgtgc 
      gtccgtgat gctgagtta tttgtattt gaaataaga 
      agcctctaa tttaatagt aataggtga gttggttaa 
      attcaacca aaacgtagt gtgggctct tcttgcacc 
      tttattgta acaaaaaaa ttccacata tctcactct 
      taatacttt aattaattg ctgttgtgt tttgagggg 
      ggatttatt ggttttatg aatttattt aaatagttt 
      aaaaaaaat tttggcttg tggggctaa ggatattct 
      aatagaatt ttaaataat tcatatttg tttagtttt 
      ttctggatt gtttttgtt ttgaagaac ggttttttt 
      tctttttag aatttattt tattgttga gggattaac 
      ttgattttt ggaatttaa tttgttaat aattaactc 
      tagaagaat tgttatgac ttttttatt tgactgaat 
      atcatttct ttttatgag atttgtatt atttatttc 
      tagtatagt aatttttta taggcgtga atatattgg 
      taggggctc caaaatgta attttaaca ttcggtcct 
      ttcgtacta gaacattaa cataaataa aagatagaa 
      accaacctg gctcacgcc ggtttaaac tcagatcat 
      gtaaaattt taaaggtcg aacagaccc aatattcca 
      gcttctaca ccgaaaatt aattttaat ccaacatcg 
      aggtcgcaa acttttctg taaatatga actttcaaa 
      aaaaattac gctgttatc cctaaggta atttaatct 
      tttaatcca aaattcagg atcaacaat tcactaatc 
      agtgtaaaa acaaaaaaa aagttaaat caattttcc 
      tatcacccc aacaaaatt ctttaaact atcaaggaa 
      aagaaaact tcaatacca ataaaaact taaaaaata 
      aaactctat agggtcttc tcgtctttt taaaaaatt 
      taagcattc tcacttaaa attcaaatt ataatacaa 
      ttaattaga gacagttaa tttctcatt aaaccattc 
      attccagct tctaattaa gaaactaat gattatgct 
      acctttgca cagtcaaaa tactgcggc cattaaaac 
      actcattgg gcaggcccg accttaaat tataatcaa 
      aaagccatg tttttaata aacaggtga aaattcaat 
      tgccgagtt tcttaaact aaccttata aataaaaat 
      taattttac aataaatta tactaattt ta
    • >TC031906 RC
      atggcataa attcagaga gtggttttg ggaacagga 
      aacaaacac gaaaaaatg aaattttga ttgaaacgc 
      cactgtttg tttttgttt atgtcttta cataaaaca 
      cgtaacctt gacctatcg tccacactt aaacaaaaa 
      actaattgg tcgctttca ggggatttg ctgggcggg 
      gagttcggc gaccaggac ataatattt cccgacacg 
      tactaagtg attttcaat gatgttccg aaaatcgac 
      ggtcctcct aagtgtgcc gaagacaac actttttgt 
      gaggacttt gattcttat ccttacaac cacgtgaaa 
      aacgtgctt aagcgcaag cgggtgcat actgattta 
      ttcggcaag gacgaaccc ctcgatgag cgctacagc 
      atcaccaac agaaacggc gacttcgag gcgttcatc 
      tgcacgagc atatccaaa acgattttc ccccgagtt 
      ggcatcaac aaaaacaac aagtggaag gtcataatc 
      aatcaagag gaagacgga tacattcaa gggattagg 
      acggaaatt tgccgaaag taaagggtt gaaagttga 
      tttctgtgt cggtgacag agtgttgcg gggaagggg 
      cggcttgtg acctcatcg gcgacaggg cgaacggct 
      acataacca gctgcaagc aaaaataca tgttgcgga 
      ggatgatgt ctttatctg aaaatgggg atcccgtcc 
      cggacacat tccagttac cgtcagcct gctgctgct 
      cttacagga aggatctga gttttttgt cccgatttg 
      gcgccgacg atgtttcct taaggtcga aagggaaag 
      gaaaataaa ataaagaat gtgatataa gttatgtga 
      ttgaacttt ttattgtaa aaacggcag cgggattat 
      ttattgaat aaaatcata cagaagcaa accacaacc 
      aatgaacga acccttctc cccacctcc ggccgaaac 
      cctaaaccc tttcaactt tcctaatca cttatttac 
      gattctatt caacattat tcttcgtgg ctgcaaacg 
      tcgaatgtg ggtgaagtg tgttttcgt ctgtgttta 
      ttttatgac tatggaagt ggttagtta tttcttttc 
      tctttttct ggattttct tatagtttt tttagtcag 
      tattcaagc aagtccagc ctggcagag taagattta 
      tcgccttga aaatgaaaa tttaattat tttttatgg 
      caggaaaca atcacatcg tttttccga actcgctag 
      aagagcatg aaactttca ttgctccaa agtgtagcg 
      gtaacaaaa cattttgtg aggacgtcg gacattacc 
      ccaaaaata aatttagcg taatattag aaaacacca 
      catacgggg aagattatt tcatgccgc aaagcaccg 
      ccgaggaag aatctatca aaaatcgat tgtacggag 
      taactgaat attacatct gcaattctc acgtccaaa 
      cgcaatatc ccaaagtgg cgcaaaacg tcagaaacg 
      agtggaaat atgtttaca attttgata actacaagc 
      aaggcgtaa ggacagaag aatgtgttg tcagatgta 
      cctgttcag aagacagta cacaacctt cagtggcag 
      tggaagaaa tgtgggttt ttgtgttgg tttttgccg 
      ttttcaaag agttgggca ttcaagcca tgtcattta 
      atccagcca agatggatg atcgcaagg gtttgacct 
      ttccggatt cgagtgaaa ttgggggtg attttttat 
      acgagacgg tggtcccca actgccaaa atcacacgt 
      actgtgaac acttggacc gctacccta acgaactat 
      tttccaaaa tattaaaaa gccgcgaaa aggagtttt 
      ctcggtact ttcagagta tggcagtaa tagagccca 
      ttatgagta ggaaccaag acacttcct gtatttgtg 
      catcacgca aaagtatta tatacccaa aagcggcat 
      ataatataa aaaataatc tgaaattta tctacaact 
      tcgatggat acaaacaag gagtgtccg tagaggtct 
      gcgtgataa aaaatcaaa tgtgcaaat tttacgaga 
      aggtgagaa agttaaaaa caaggtgtc gacaaaagt 
      tcgtcgaaa aattgttac tgacggctg atgaaaatg 
      gaagaccgt acgctgata gttgggtaa ctgcagagt 
      aatacattt actttgtgg agaagtgaa ctgagacga 
      ttttatccc tgtaattat cgcagtatt aggtggaag 
      aaaacgata ggtattaat cacaattta gtgaatccc 
      agctaataa ttcttgaaa agtaattac gtgtgattt 
      gtgtgatta tgacgaatt gtaggtacg taagttatt 
      gtggactaa tgtgtgttt ttataatta aattttagc 
      aattttagt ccggacgtt agtcaagtt tttatttat 
      tgttacaag ctttaccag tgtctgaaa ctgtttaag 
      cattttatg aaatattat gttagaact agcttaagc 
      attaaaaat gtacaaaca gacgaaaag tattgtagt 
      attgtaaat ttatgtgta ctataaact gtaattaat 
      aaagctgtt gatgttcga atgaactta ttttatgtg 
      ggaaaataa ataaaattt gttgcaaac aggaaagct 
      gttaataac tgaaactta aatgaattt ccttcagtt 
      ttgtttgag gaacggtgt ggtctgatg tgcgtccgt 
      gatgctgag ttatttgta tttgaaata agaagcctc 
      taatttaat agtaatagg tgagttggt taaattcaa 
      ccaaaacgt agtgtgggc tcttcttgc acctttatt 
      gtaacaaaa aaattccac atatctcac tcttaatac 
      tttaattaa ttgctgttg tgttttgag gggggattt 
      attggtttt atgaattta tttaaatag tttaaaaaa 
      aattttggc ttgtggggc taaggatat tctaataga 
      attttaaat aattcatat ttgtttagt tttttctgg 
      attgttttt gttttgaag aacggtttt ttttctttt 
      tagaattta ttttattgt tgagggatt aacttgatt 
      tttggaatt taatttgtt aataattaa ctctagaag 
      aattgttat gactttttt atttgactg aatatcatt 
      tctttttat gagatttgt attatttat ttctagtat 
      agtaatttt ttataggcg tgaatatat tggtagggg 
      ctccaaaat gtaatttta acattcggt cctttcgta 
      ctagaacat taacataaa taaaagata gaaaccaac 
      ctggctcac gccggttta aactcagat catgtaaaa 
      ttttaaagg tcgaacaga cccaatatt ccagcttct 
      acaccgaaa attaatttt aatccaaca tcgaggtcg 
      caaactttt ctgtaaata tgaactttc aaaaaaaat 
      tacgctgtt atccctaag gtaatttaa tcttttaat 
      ccaaaattc aggatcaac aattcacta atcagtgta 
      aaaacaaaa aaaaagtta aatcaattt tcctatcac 
      cccaacaaa attctttaa actatcaag gaaaagaaa 
      acttcaata ccaataaaa acttaaaaa ataaaactc 
      tatagggtc ttctcgtct ttttaaaaa atttaagca 
      ttctcactt aaaattcaa attataata caattaatt 
      agagacagt taatttctc attaaacca ttcattcca 
      gcttctaat taagaaact aatgattat gctaccttt 
      gcacagtca aaatactgc ggccattaa aacactcat 
      tgggcaggc ccgacctta aattataat caaaaagcc 
      atgttttta ataaacagg tgaaaattc aattgccga 
      gtttcttaa actaacctt ataaataaa aattaattt 
      tacaataaa ttatactaa tttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY