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Gene information
General information
TC010320 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451153.1: 8996..23619
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010320
    iBeetle screen iB_09572

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010320 RA
      MAVGSVKPL SLPTPFRPH DYAYDPLFV VSGPKDHYK 
      AALLARLST AKFQICPIF ANMFSDLPN HPRKQYIRR 
      KSAHLPQAG RSYKSRLEP NLDYLCSRK VDVIGADRS 
      KYFHVPKTS IGGHLIPEL QLHYTSPRE KLPSVVKAE 
      PKSQNKKIQ TEFRESSVQ TSPWEPSIV FNGDTEPEL 
      LKLDFLKWG SGLPPGMHE VQLLERARM KRAWEERCE 
      VRTAEDLEK RRTIIAAME RDEWAFREQ EIQDIQDLR 
      LKLLEKMLN ELHEKSKNR NEAKLRMFA EMKYAEKEQ 
      KLKKLRDKT KRELRKLDL KHAGIDQKY HSVNVVEEH 
      INHASEIYG PLMRHGEHP KRWHLIIDE ALTRFKAQF 
      VGVEEFNTL PTWLNKATK IKDCMCLKM KGTRLCMRE 
      TKWTAPVLK KLHEELLAL RKHDLKPCT LIKKIQSPP 
      SAISTPEVE GISDGDEDF YQAMVMIQS VIKGRAAQM 
      HIYEGRERC RELIQELKF SSGLLVEEN VQIHDYKLK 
      IKLQQREEQ IALEKLFRL RESLDELQS TVVGSLLDF 
      LNKELRRLL EERRAHAIC ILLERERYD REAAEAGRR 
      QVELRRRRE HDEMFKQIV KIQQETVDL YLEDIIKES 
      MEFTSEEEA RSYVEGMAR KIDEEAYRI KEATNITIN 
      DEEEIVADL VHHFVLPEV QKQIIRKKI QRKQREKLR 
      TVHDTVYSE IEDVNVTQE PEPSEQSSL KEYPVVGSS 
      QKFRQHSVP GTSTEDRHF SIIHEATND TTSHASSDG 
      KSHKNSKCQ RPDV
    CDS sequence
    • >TC010320 RA
      atggcagtg ggatcagtt aaaccatta tcactacct 
      accccattt aggccccat gactacgct tacgatccc 
      ttatttgtg gtctctgga cccaaagac cactacaaa 
      gctgcttta ttggcaaga ctatcaaca gcgaaattc 
      caaatatgt ccaattttt gccaacatg ttctcggat 
      ttgccaaac cacccgcgc aaacaatac attcgacgc 
      aaatccgcg cacttacct caagctggc agaagctac 
      aaatccaga cttgaaccg aatcttgat tatttatgt 
      tcgaggaaa gtggatgtg attggagca gatcgctcg 
      aaatatttc cacgttccc aaaactagc attgggggg 
      catttaatt ccagaactt cagctccac tatacgtca 
      ccaagagaa aaattaccc tctgttgtt aaagcggag 
      ccgaagagt caaaataag aaaattcaa actgagttc 
      agggaatcg tcggttcaa acttctcca tgggagccc 
      tcgattgtg tttaatgga gatactgag cccgaattg 
      ttgaaactt gactttctc aaatggggt agcggtttg 
      ccgcccggc atgcacgaa gtgcagctc cttgaaaga 
      gccaggatg aagagagct tgggaggaa agatgtgaa 
      gtgagaact gctgaggat ttagaaaaa cggaggact 
      attattgcg gcgatggaa cgagatgag tgggctttt 
      agagaacaa gaaatacaa gatattcaa gaccttcga 
      cttaaactt ttagaaaaa atgttaaat gagcttcac 
      gaaaaatca aagaataga aatgaggcc aagttgaga 
      atgtttgcc gagatgaaa tatgccgag aaagaacaa 
      aaactgaag aaattgcgt gataaaaca aaacgagaa 
      ctgcgaaaa ttagattta aaacatgcg ggcattgac 
      cagaaatac cactctgtc aacgttgtt gaagaacac 
      atcaaccac gcatccgaa atttacggt cctctgatg 
      aggcacggt gaacatccg aaacgttgg cacttaata 
      atcgacgaa gccctcaca cgattcaaa gcccaattt 
      gtcggtgtt gaagagttc aacacttta ccaacttgg 
      ttgaataaa gcaaccaaa attaaagac tgcatgtgt 
      ttgaaaatg aaaggcaca cgtctctgc atgagagaa 
      acaaaatgg acagctcct gtgctgaaa aagcttcat 
      gaggagctt ttagcacta cgaaagcac gatcttaaa 
      ccttgcact ctaattaag aaaattcaa agcccaccc 
      tctgcaatc agcactcca gaagttgaa ggcatttcg 
      gacggtgat gaagatttt taccaagcg atggttatg 
      atacaatcg gttatcaaa ggacgagcg gctcaaatg 
      catatttac gaaggtcgg gaaagatgt agagaatta 
      atccaagaa ctgaaattt tcaagcggt cttttggtg 
      gaagaaaac gtccaaata cacgactac aaactaaaa 
      ataaaactc caacaaaga gaggaacaa atcgcactg 
      gaaaagctt ttcagattg cgtgaaagt ctagacgag 
      cttcaaagt acagtagtt ggatctttg ttggacttt 
      cttaataaa gagttgcgg cgattgctt gaagaacgc 
      cgtgctcat gcaatttgt attcttctt gaacgtgag 
      agatatgat agagaagcg gctgaagct ggtcgcaga 
      caagtggag ctgagacga agacgtgaa cacgatgaa 
      atgttcaag caaattgtt aaaatccaa caggaaact 
      gtcgatttg tatcttgag gatattatt aaggaaagt 
      atggaattt acgtcggag gaagaagcg agaagttat 
      gttgaagga atggcgagg aaaatcgac gaagaggcg 
      tatagaata aaggaggca accaatata accatcaac 
      gacgaagaa gaaatcgtg gcggacctc gtccaccac 
      ttcgtctta ccagaggtt cagaagcaa ataatacgc 
      aaaaaaatc cagcgaaaa caacgcgaa aaattaaga 
      acagtccac gacacagtt tactccgaa atcgaagac 
      gtgaacgtc actcaagag ccggaacca tccgaacaa 
      tcgagtctt aaggagtat cctgttgtt ggaagcagc 
      caaaagttt cgacaacac agcgttcca gggactagt 
      actgaagac agacacttc agtataata catgaagcg 
      acaaacgac accacttca catgccagc tccgatgga 
      aagagtcac aagaatagc aagtgccag agaccagac 
      gtctaa
    mRNA sequence
    • >TC010320 RA
      tgacaaatt cactcattt ttggtttat ttctgaatg 
      aaaaataaa gcgcaatgg cagtgggat cagttaaac 
      cattatcac tacctaccc catttaggc cccatgact 
      acgcttacg atcccttat ttgtggtct ctggaccca 
      aagaccact acaaagctg ctttattgg caagactat 
      caacagcga aattccaaa tatgtccaa tttttgcca 
      acatgttct cggatttgc caaaccacc cgcgcaaac 
      aatacattc gacgcaaat ccgcgcact tacctcaag 
      ctggcagaa gctacaaat ccagacttg aaccgaatc 
      ttgattatt tatgttcga ggaaagtgg atgtgattg 
      gagcagatc gctcgaaat atttccacg ttcccaaaa 
      ctagcattg gggggcatt taattccag aacttcagc 
      tccactata cgtcaccaa gagaaaaat taccctctg 
      ttgttaaag cggagccga agagtcaaa ataagaaaa 
      ttcaaactg agttcaggg aatcgtcgg ttcaaactt 
      ctccatggg agccctcga ttgtgttta atggagata 
      ctgagcccg aattgttga aacttgact ttctcaaat 
      ggggtagcg gtttgccgc ccggcatgc acgaagtgc 
      agctccttg aaagagcca ggatgaaga gagcttggg 
      aggaaagat gtgaagtga gaactgctg aggatttag 
      aaaaacgga ggactatta ttgcggcga tggaacgag 
      atgagtggg cttttagag aacaagaaa tacaagata 
      ttcaagacc ttcgactta aacttttag aaaaaatgt 
      taaatgagc ttcacgaaa aatcaaaga atagaaatg 
      aggccaagt tgagaatgt ttgccgaga tgaaatatg 
      ccgagaaag aacaaaaac tgaagaaat tgcgtgata 
      aaacaaaac gagaactgc gaaaattag atttaaaac 
      atgcgggca ttgaccaga aataccact ctgtcaacg 
      ttgttgaag aacacatca accacgcat ccgaaattt 
      acggtcctc tgatgaggc acggtgaac atccgaaac 
      gttggcact taataatcg acgaagccc tcacacgat 
      tcaaagccc aatttgtcg gtgttgaag agttcaaca 
      ctttaccaa cttggttga ataaagcaa ccaaaatta 
      aagactgca tgtgtttga aaatgaaag gcacacgtc 
      tctgcatga gagaaacaa aatggacag ctcctgtgc 
      tgaaaaagc ttcatgagg agcttttag cactacgaa 
      agcacgatc ttaaacctt gcactctaa ttaagaaaa 
      ttcaaagcc caccctctg caatcagca ctccagaag 
      ttgaaggca tttcggacg gtgatgaag atttttacc 
      aagcgatgg ttatgatac aatcggtta tcaaaggac 
      gagcggctc aaatgcata tttacgaag gtcgggaaa 
      gatgtagag aattaatcc aagaactga aattttcaa 
      gcggtcttt tggtggaag aaaacgtcc aaatacacg 
      actacaaac taaaaataa aactccaac aaagagagg 
      aacaaatcg cactggaaa agcttttca gattgcgtg 
      aaagtctag acgagcttc aaagtacag tagttggat 
      ctttgttgg actttctta ataaagagt tgcggcgat 
      tgcttgaag aacgccgtg ctcatgcaa tttgtattc 
      ttcttgaac gtgagagat atgatagag aagcggctg 
      aagctggtc gcagacaag tggagctga gacgaagac 
      gtgaacacg atgaaatgt tcaagcaaa ttgttaaaa 
      tccaacagg aaactgtcg atttgtatc ttgaggata 
      ttattaagg aaagtatgg aatttacgt cggaggaag 
      aagcgagaa gttatgttg aaggaatgg cgaggaaaa 
      tcgacgaag aggcgtata gaataaagg aggcaacca 
      atataacca tcaacgacg aagaagaaa tcgtggcgg 
      acctcgtcc accacttcg tcttaccag aggttcaga 
      agcaaataa tacgcaaaa aaatccagc gaaaacaac 
      gcgaaaaat taagaacag tccacgaca cagtttact 
      ccgaaatcg aagacgtga acgtcactc aagagccgg 
      aaccatccg aacaatcga gtcttaagg agtatcctg 
      ttgttggaa gcagccaaa agtttcgac aacacagcg 
      ttccaggga ctagtactg aagacagac acttcagta 
      taatacatg aagcgacaa acgacacca cttcacatg 
      ccagctccg atggaaaga gtcacaaga atagcaagt 
      gccagagac cagacgtct aaaactaag cacccgcac 
      acagaagat aaatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY