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Gene information
General information
TC005067 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7134612..7137038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005067
    iBeetle screen iB_09567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005067 RA
      MDALHRIDC SLSPISIDQ IEPNLFLGS LSAAKDVDT 
LARYKITHI LTIDTCPLP RKILELKHI TTKYIQLSD 
QPKEDLLSH FDDAGAFIL EGVTKGAVL VHCYFGVSR 
SASVVIAYV MKKYELSYK EAFEKVKAK RGLVYPNHG 
FVSQLHLYK EMGYKIDPN NMKYKLFRL NVAANHVKK 
VKILPQNFM DLIKFDPGL TQTQPEPNV YRCKKCRRV 
LASESNLMT HKVGGEVCT KTYFLEPLA WMNVTQTTQ 
DKLYCPKCN SKVGSFSWI MGCLCPCGV QVAPAFYLT 
PSKVDFTNV VKNVEMTF
    CDS sequence
    • >TC005067 RA
      atggacgca ttacacagg attgactgc agcttgtcg 
cctatcagt atcgatcaa atcgaacca aatttattt 
cttggttca ttgtctgca gccaaagat gtggacact 
ctggcaagg tacaaaata acccacatt ctgacaata 
gacacctgt cctttgcct cgaaaaatc ctggaactg 
aaacacatc accacgaaa tacatccag ctgtctgat 
caacccaag gaagattta ttgagtcac tttgacgat 
gctggggct tttattctc gaaggtgtg accaaaggg 
gctgtattg gtgcactgc tacttcggc gtctcccga 
agcgccagt gttgttata gcctatgtc atgaaaaaa 
tacgaattg agttacaag gaggctttt gagaaggtc 
aaagcgaag aggggttta gtgtacccc aaccacggt 
tttgtgtct caactccac ttgtacaag gaaatgggc 
tacaaaatc gacccaaac aacatgaag tacaagctg 
tttagatta aacgtcgcc gctaatcac gtgaagaaa 
gtgaaaatc cttccacaa aacttcatg gatttgatc 
aaatttgat cccgggttg actcaaacg cagcctgag 
cccaacgtc tacagatgc aagaagtgt cgaagagtt 
ttggcgtcc gagtccaat ctgatgacg cacaaggtt 
gggggcgag gtttgtacc aaaacttat tttcttgaa 
ccgctggct tggatgaat gtcactcag acaacccag 
gataagttg tattgtcca aagtgtaac agtaaagtt 
ggctcgttt agctggatc atgggttgt ctgtgccct 
tgtggggtc caagtggcc ccggccttt tatttgaca 
ccatccaag gttgatttc accaatgtt gtgaagaac 
gttgaaatg actttttaa
    mRNA sequence
    • >TC005067 RA
      tcacaggct ttgaaacaa ttgacatta aagaattcg 
gtcgctttg taaatgtta gatgtcaat cagaatcaa 
aaataaata aatcagaat ttttacaac aaagtttga 
tttctaaga cgattgtta ttgccgtgg aggaaggga 
gagacgata gtgtgggtg acgcaatat tgtctatat 
ttagggcta aaatgatca aagataacc ttatcaaag 
tgtggcagt tgaaggttt aagtggcga aatggacgc 
attacacag gattgactg cagcttgtc gcctatcag 
tatcgatca aatcgaacc aaatttatt tcttggttc 
attgtctgc agccaaaga tgtggacac tctggcaag 
gtacaaaat aacccacat tctgacaat agacacctg 
tcctttgcc tcgaaaaat cctggaact gaaacacat 
caccacgaa atacatcca gctgtctga tcaacccaa 
ggaagattt attgagtca ctttgacga tgctggggc 
ttttattct cgaaggtgt gaccaaagg ggctgtatt 
ggtgcactg ctacttcgg cgtctcccg aagcgccag 
tgttgttat agcctatgt catgaaaaa atacgaatt 
gagttacaa ggaggcttt tgagaaggt caaagcgaa 
gaggggttt agtgtaccc caaccacgg ttttgtgtc 
tcaactcca cttgtacaa ggaaatggg ctacaaaat 
cgacccaaa caacatgaa gtacaagct gtttagatt 
aaacgtcgc cgctaatca cgtgaagaa agtgaaaat 
ccttccaca aaacttcat ggatttgat caaatttga 
tcccgggtt gactcaaac gcagcctga gcccaacgt 
ctacagatg caagaagtg tcgaagagt tttggcgtc 
cgagtccaa tctgatgac gcacaaggt tgggggcga 
ggtttgtac caaaactta ttttcttga accgctggc 
ttggatgaa tgtcactca gacaaccca ggataagtt 
gtattgtcc aaagtgtaa cagtaaagt tggctcgtt 
tagctggat catgggttg tctgtgccc ttgtggggt 
ccaagtggc cccggcctt ttatttgac accatccaa 
ggttgattt caccaatgt tgtgaagaa cgttgaaat 
gacttttta agtacctga tacgtgaga aacaataat 
attatccca gaaaagtct tccaacttt ttttgtgat 
tcttgaacg gaaggaagc cttttcaaa taaattcat 
tttgggatt tgtatcaag ccaaataat cagtgcttc 
attattaat tacaataat ttcatgttg tgttttgtg 
gcaaaattg tgatattgt tataaatat ggtgtgatc 
aaaaatttc cttgaagtt tggatgcct tcataattt 
tcgaggaaa ataattagg gctgtctcc ggtaccggc 
acactagtt tgactccca taactctcc tacttttaa 
tttttcgtt tgagattgc tcacagtta ctgagaaaa 
ttgttgtaa tagacaaaa aggaccact ttctgaata 
cttgataac tttgaattt attaagttt gtcgacgcc 
aatcgactt cttggaacg ttttgcatt tcaatttca 
aattttgtt taaaaaaca aagattttt cgtaagctt 
attttaaat tattaaaac ttaaaaaaa tatgctagt 
taaacaaaa aaattccac ggtttttct cgttatctg 
aactgtact ctgtactgc tattactat ttaatgcca 
agattttaa atttgttgg gatggtcac tttagttct 
aggtgaaaa gattagaac tcggaaact caaatttcc 
gctcgatat ttcgaaaat gattcgtca tacattaaa 
ctggcggtc tggttataa ttaaaaaaa tttttgaat 
tatttttac ggcttaatt ttgatcgat cgttgtaga 
atttttcaa gaattcaca cctttcaaa aaacgatat 
ttttatttt tgtgaggct ttagggctc ctaaaggtg 
caaaaacga caagttttc accgatttt cagttttgc 
caacatcgg aggcatgcc aagtaatta ctactgttc 
aacgcacat aatttgaaa tttttgatg gcactatat 
ttgtaggat aagaaagta ttaaagtct atttttgtg 
taatttttg gctccaaca ggagtaacg taataatgt 
taatttaaa actcgttgt tataaggta acataaaca 
aatgctaga gcaatactt ttaatgaat ataaaaaaa 
aacagtgat acaataatc aacacttca ctgaacata 
aatacagta ttgaccttt ccaggtatt atattgatg 
gccaaaaac agataaaag aattaaatt gtacaacct 
aca
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