Status
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Gene information
General information
TC001976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 46935..50622
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001976
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09566

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    vertex triplet vertex triplet anterior not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001976 RA
      MKLVVLLNA LCIGLSLCF NGQLAELEC PDDCDCHYF 
      RINWVTDCS DSNLTEIPY DELSLSVYV LDLNNNQIT 
      DVGPFPHDI KMRRLQLAD NLMTELKKE SFAGLNYLI 
      DADFSGNMI TRVEPDCFD DSPGFITLE LQGNPLEPV 
      DGIFLSIKS LLYLDISNC GLKYLNPLF FANITALST 
      LDLSGNPLG VIDNKVFEP LSSLETLKM NGCNLTYIA 
      NYAFNSLEN LKNLELSAN MLTTDWSSV LDYLPRLEY 
      LDLRNSQIR HLPESVFRN NTYLRTLIL AQNELNDFD 
      VSQTVGKLQ QLDSLDLSF CNLTLPLSE DAFVNATKI 
      RSLFLSGNS LFASDLLVA LAPLSNLER LSLSNCGLT 
      RLPDTFRNF KSLQELDIS HNPLNDAFV KLLTPLDKL 
      EYLNMGYSN LSYIAPTSF SKMTNMKRL VLSGNDLNN 
      LEAGLFGNL TRLESLELN SCGLRRSIN ATLFFNNLT 
      YTDLTELQL AGNPLQVSK SGPLLPRQL SRLLTLDLS 
      NCNLTFLPP DAFYWTRNI TTLILSGNH FSSADNLQF 
      LELLPKLKV LDLRHNKLS SFSPKTIYL NPNVEKLKL 
      IGNPWRCDC TVAELWDWA NLEKGSLGI LEGSTIAAE 
      HVTVGKTNK RKKLLVCNY DTSQPLPII TKTVRGRRP 
      FIKPQRVLT STNRTWAKY VRESGCEQL PVLQRSPRA 
      ADLNYRRSE LEPNTWAAA AISAAAVYI TLMTIVGIA 
      FVFTKKRRT PRTTPDKNN
    CDS sequence
    • >TC001976 RA
      atgaagttg gtggtctta ttaaacgct ctatgcatc 
      ggtctcagt ttgtgtttt aatggtcag ttggcggaa 
      cttgaatgt ccggacgat tgcgattgc cattacttc 
      cggatcaat tgggtgacg gattgttcc gatagcaac 
      ctaaccgag ataccgtac gatgagttg agtctgagt 
      gtttacgtc ttggacttg aacaacaat caaataact 
      gacgtcggg ccgtttccg cacgacatc aaaatgcgg 
      cggttgcag ctcgcggac aatctcatg actgagctg 
      aagaaggag tcgttcgca ggacttaac tacctgatc 
      gatgccgat ttttcggga aatatgata accagagtc 
      gagcctgat tgcttcgat gattcgccg ggctttatc 
      actttagaa cttcaaggg aatccgcta gagccggtc 
      gatgggatc tttttatcc atcaagtca ttgctttat 
      ttggacatt agcaactgc gggctaaaa tatttaaat 
      ccacttttc ttcgccaat attaccgca ctgagtacg 
      ctcgactta tccggaaat cccttaggc gtgatagat 
      aataaagtc ttcgagcct ttgtctagt ctggaaacg 
      ctgaaaatg aacggctgc aatttgact tacattgca 
      aattacgcc ttcaattct ttggaaaat ctaaaaaat 
      ctagaatta agcgctaac atgcttact actgattgg 
      tcgtcagtc ctcgattat ctcccccga ttggagtat 
      ctcgatctc cggaattca caaattcgt cacttgccg 
      gaatctgtc ttccgtaac aacacttat ctaaggacg 
      ctaattctg gcccagaat gagctcaac gattttgat 
      gtatcacaa accgtcggc aaactccaa caactggac 
      tcactagat ctgtctttc tgcaattta acattgcct 
      ctatcggaa gatgctttc gtgaacgcg acgaaaatt 
      cgtagtttg ttcctatca ggaaattcc ctattcgcg 
      tccgactta ttggttgct ttggctcca ttatcaaat 
      ctagagcga ctctcttta agtaattgt ggattaact 
      cgtctaccg gatactttc cgtaacttc aaaagtcta 
      caagaactc gatatttcc cataatcct ctcaatgac 
      gcattcgtc aaattattg acgcctctc gataaatta 
      gaatactta aatatgggt tacagtaat ttatcatac 
      atagccccg acgtcattc tcaaaaatg acaaatatg 
      aaacgatta gtactttcc ggaaacgac ttgaataac 
      ttagaggcc ggtcttttt ggcaactta actagactc 
      gaaagttta gaattaaat tcttgcggc ttaagacga 
      tcgatcaac gcaacgtta ttcttcaat aacttgaca 
      tacaccgat ttaaccgaa ctacaattg gccggaaat 
      ccgctccaa gtatcgaaa tcaggccct cttttacct 
      cgacaatta tcaagactg ctaacttta gatctgagc 
      aattgtaat ttgacgttc ctgccaccg gatgcgttc 
      tattggact agaaatatc accacattg attttaagc 
      ggaaaccat ttctcctca gccgataat ttacaattc 
      cttgaatta ctacctaaa ctaaaagtg ctcgatttg 
      aggcacaat aaattatcc tcattctct ccgaagaca 
      atctattta aatcccaac gtggaaaaa ctaaaacta 
      atcggcaat ccctggagg tgcgactgc accgttgcc 
      gaattatgg gattgggcc aatttggaa aagggcagt 
      ctcggcatt ttggagggc tcaaccatc gcagccgag 
      cacgtgacg gtgggcaaa accaacaag cgcaaaaaa 
      ctgcttgtg tgcaattac gacacctcg cagcctttg 
      cccattatc accaaaacg gttcgcggc cgcagaccc 
      ttcatcaag ccgcaaagg gttctaacc tccaccaac 
      cgtacgtgg gccaaatat gtgcgcgaa tcgggatgc 
      gagcaactc cctgttttg caaaggtct ccacgtgcc 
      gctgatctg aattaccgt agatcggaa ttagagccc 
      aacacttgg gccgcggcc gccatcagc gctgccgct 
      gtctatatc accctaatg acgatcgtc ggaattgcc 
      tttgttttt acgaagaag cggcgaacg cccagaaca 
      acccccgac aaaaataat taa
    mRNA sequence
    • >TC001976 RA
      ccttcgtga atgtcgaag cttccactc ctgctgctc 
      ctgcaaaat ggatcgacg ctcgtgcat tattcagct 
      cagttaaca tttgtggcg ttgtcatgc gtttggtga 
      gctttgtga agtgcatat tggtgcagt atccgaacg 
      tttgtcgtc acgtctgaa tttgcgagg atgaagttg 
      gtggtctta ttaaacgct ctatgcatc ggtctcagt 
      ttgtgtttt aatggtcag ttggcggaa cttgaatgt 
      ccggacgat tgcgattgc cattacttc cggatcaat 
      tgggtgacg gattgttcc gatagcaac ctaaccgag 
      ataccgtac gatgagttg agtctgagt gtttacgtc 
      ttggacttg aacaacaat caaataact gacgtcggg 
      ccgtttccg cacgacatc aaaatgcgg cggttgcag 
      ctcgcggac aatctcatg actgagctg aagaaggag 
      tcgttcgca ggacttaac tacctgatc gatgccgat 
      ttttcggga aatatgata accagagtc gagcctgat 
      tgcttcgat gattcgccg ggctttatc actttagaa 
      cttcaaggg aatccgcta gagccggtc gatgggatc 
      tttttatcc atcaagtca ttgctttat ttggacatt 
      agcaactgc gggctaaaa tatttaaat ccacttttc 
      ttcgccaat attaccgca ctgagtacg ctcgactta 
      tccggaaat cccttaggc gtgatagat aataaagtc 
      ttcgagcct ttgtctagt ctggaaacg ctgaaaatg 
      aacggctgc aatttgact tacattgca aattacgcc 
      ttcaattct ttggaaaat ctaaaaaat ctagaatta 
      agcgctaac atgcttact actgattgg tcgtcagtc 
      ctcgattat ctcccccga ttggagtat ctcgatctc 
      cggaattca caaattcgt cacttgccg gaatctgtc 
      ttccgtaac aacacttat ctaaggacg ctaattctg 
      gcccagaat gagctcaac gattttgat gtatcacaa 
      accgtcggc aaactccaa caactggac tcactagat 
      ctgtctttc tgcaattta acattgcct ctatcggaa 
      gatgctttc gtgaacgcg acgaaaatt cgtagtttg 
      ttcctatca ggaaattcc ctattcgcg tccgactta 
      ttggttgct ttggctcca ttatcaaat ctagagcga 
      ctctcttta agtaattgt ggattaact cgtctaccg 
      gatactttc cgtaacttc aaaagtcta caagaactc 
      gatatttcc cataatcct ctcaatgac gcattcgtc 
      aaattattg acgcctctc gataaatta gaatactta 
      aatatgggt tacagtaat ttatcatac atagccccg 
      acgtcattc tcaaaaatg acaaatatg aaacgatta 
      gtactttcc ggaaacgac ttgaataac ttagaggcc 
      ggtcttttt ggcaactta actagactc gaaagttta 
      gaattaaat tcttgcggc ttaagacga tcgatcaac 
      gcaacgtta ttcttcaat aacttgaca tacaccgat 
      ttaaccgaa ctacaattg gccggaaat ccgctccaa 
      gtatcgaaa tcaggccct cttttacct cgacaatta 
      tcaagactg ctaacttta gatctgagc aattgtaat 
      ttgacgttc ctgccaccg gatgcgttc tattggact 
      agaaatatc accacattg attttaagc ggaaaccat 
      ttctcctca gccgataat ttacaattc cttgaatta 
      ctacctaaa ctaaaagtg ctcgatttg aggcacaat 
      aaattatcc tcattctct ccgaagaca atctattta 
      aatcccaac gtggaaaaa ctaaaacta atcggcaat 
      ccctggagg tgcgactgc accgttgcc gaattatgg 
      gattgggcc aatttggaa aagggcagt ctcggcatt 
      ttggagggc tcaaccatc gcagccgag cacgtgacg 
      gtgggcaaa accaacaag cgcaaaaaa ctgcttgtg 
      tgcaattac gacacctcg cagcctttg cccattatc 
      accaaaacg gttcgcggc cgcagaccc ttcatcaag 
      ccgcaaagg gttctaacc tccaccaac cgtacgtgg 
      gccaaatat gtgcgcgaa tcgggatgc gagcaactc 
      cctgttttg caaaggtct ccacgtgcc gctgatctg 
      aattaccgt agatcggaa ttagagccc aacacttgg 
      gccgcggcc gccatcagc gctgccgct gtctatatc 
      accctaatg acgatcgtc ggaattgcc tttgttttt 
      acgaagaag cggcgaacg cccagaaca acccccgac 
      aaaaataat taaacgcga gagattttt tcctgtagt 
      ttagttgtg atacatcca atcgttcct taatagtgc 
      ccaaggttt agccaacag aggctggtt ttctccaag 
      acgtaataa taaacttcc gttaattgt gttaatcac 
      gagtcagca atttcatag aaaacaatt atagagatg 
      taaacaata agtgtgaaa ggtatttgt ttaattacg 
      tagacgtgt taatcggag aactgcgtt ttttcaaga 
      ctgtttttt gttgatttt gtatttaac tgggtgtgg 
      ctgttattt tctatgcta ataaactat tcatttata 
      ataaacgtg gttgttttg cgcacctat ttgtgtgat 
      cccctccgt atcacaata actatgcaa attggtgcg 
      tgcactacg atctttttt tctatgacc cttcgaatt 
      atgttcgtt gcccaaggc gatttttaa attgaactc 
      cttttagta aagggaata tgtaatgct ataccggct 
      ataactcga aaatggcca cgaccacct cttaccctg 
      tcatagagg gcgggtttc cagcagttc ctgtaattg 
      caagtggaa cgtgaacag cccttgttt cggcccttc 
      tgcctctcc taattagaa cacgcgccc cttaaaccc 
      aaccaccta ctcgccgat aacagggac acctacagg 
      atggaccga aactaattt tcgaaaaat ttaacgaga 
      tgatgtcaa cacgacaat tttcatatt atcttaaca 
      aggaaattg taggctgat tttatgaat ttatttaca 
      tggtagacg cgtcccttt tttcctatt gtagttgat 
      aagtgcatc ttgttttat ttttgtact gcaattgcg 
      taattagat tgttgtaat ttgtcacag tattaaaac 
      gtacctttt tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY