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Gene information
General information
TC004294 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451971.1: 63661..66213
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004294
    iBeetle screen iB_09562

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004294 RA
      MAWLQNLAG RAEDLLNKI DQNAATVLN DSKLIDENI 
      MPSDLDEKT YEETINLTG PQSDTIDSI KPTFEEELC 
      VVEGHDYDL EKLSKGDSP GPSISSHNS FVLTEDVQS 
      YTDTINKLE IELQDLNKQ LLNLHHLYA ELRNENANL 
      RSQVEASNY LVATAQSEM EQYKARAQR ILQEKDDLI 
      RLKNENKSS QNDQILTNY HDELKKEIE FQQSRNVEL 
      SEKVKKLTH EISHLQQQM VLSQNSSQQ STQLLQENL 
      TREQKIRLM AEDECRVKN QELQLKLQE LAQQYQVIQ 
      SKSDEIAKL QDALKSKPS TDFESRIKS LTDTLMVKQ 
      NALERVTTE RNALRIQLE KLETEYHNN LAQLERAQV 
      RVINVNDTD DVKSQVPQF MRVSPFDAG VTRRVKHAY 
      STVDAISVR TGIFLRRYP LARVFVFCY MVLLHFWAL 
      ILLFLYAPS SR
    CDS sequence
    • >TC004294 RA
      atggcttgg ttgcaaaat ttggcaggt cgtgccgaa 
      gacttgttg aataaaatt gaccaaaat gcggcgacc 
      gttctaaac gatagtaaa ttaattgac gaaaacata 
      atgccgtca gatctggac gaaaaaact tatgaggaa 
      acaattaat ctgactgga ccacaaagc gacacaatt 
      gattcaatt aaacccacg tttgaggag gagttatgt 
      gtggttgaa ggtcacgat tacgattta gagaaatta 
      tcgaagggg gattcgccg ggcccgtca atatcgtca 
      cacaatagt ttcgttttg actgaagac gtgcaaagc 
      tacactgac acaataaac aagcttgaa attgagcta 
      caagacctc aataagcaa ctcttaaat ttgcaccat 
      ttgtatgcc gaacttcgc aatgaaaat gcaaacttg 
      cgatcacaa gttgaagcc tcgaattac ttagtcgca 
      acagcgcag tccgaaatg gagcagtac aaagcccgg 
      gcccaacgt attttgcaa gagaaggac gatttaatc 
      cgcttgaaa aacgagaat aaatcgtcg caaaacgac 
      caaatttta accaattat cacgacgag ttaaaaaaa 
      gagatcgag tttcagcaa agccgcaac gtggaattg 
      agcgagaaa gtgaagaag ctaacgcat gaaatttcc 
      cacttgcaa caacaaatg gttttgtcc cagaattcg 
      tcgcaacaa tcaacgcaa ctcctccaa gagaatcta 
      acaagggaa cagaagatc cggcttatg gccgaggat 
      gagtgtcgg gttaaaaac caagagttg cagttgaaa 
      ttgcaagaa ttggcgcaa cagtaccaa gtcatccaa 
      tcaaaatcg gacgaaatc gccaagttg caagacgcc 
      ctcaagtcg aaacccagc accgacttt gagtcgcgg 
      attaagtcc ctcactgat acgctcatg gtgaagcaa 
      aatgcctta gaacgagtt acaaccgaa cgcaacgcg 
      ttgcgaatc caactagag aagctggaa actgagtat 
      cacaataat ttggcgcag cttgagcgc gcccaagtg 
      cgtgtcatc aacgtgaat gatacagat gatgttaaa 
      tcgcaagtt ccgcaattt atgcgcgtg tctccgttc 
      gatgctggg gtcacaaga cgggtcaaa cacgcgtat 
      tcgactgtt gatgcgatc agtgttagg acggggatt 
      ttcctgcgg cggtacccc ctggccagg gtttttgtt 
      ttctgttat atggtcttg ttgcacttt tgggccttg 
      attctactc ttcctgtat gccccaagc agccgttaa
    mRNA sequence
    • >TC004294 RA
      taataaatg gtaaatttt ggtcatggc ttggttgca 
      aaatttggc aggtcgtgc cgaagactt gttgaataa 
      aattgacca aaatgcggc gaccgttct aaacgatag 
      taaattaat tgacgaaaa cataatgcc gtcagatct 
      ggacgaaaa aacttatga ggaaacaat taatctgac 
      tggaccaca aagcgacac aattgattc aattaaacc 
      cacgtttga ggaggagtt atgtgtggt tgaaggtca 
      cgattacga tttagagaa attatcgaa gggggattc 
      gccgggccc gtcaatatc gtcacacaa tagtttcgt 
      tttgactga agacgtgca aagctacac tgacacaat 
      aaacaagct tgaaattga gctacaaga cctcaataa 
      gcaactctt aaatttgca ccatttgta tgccgaact 
      tcgcaatga aaatgcaaa cttgcgatc acaagttga 
      agcctcgaa ttacttagt cgcaacagc gcagtccga 
      aatggagca gtacaaagc ccgggccca acgtatttt 
      gcaagagaa ggacgattt aatccgctt gaaaaacga 
      gaataaatc gtcgcaaaa cgaccaaat tttaaccaa 
      ttatcacga cgagttaaa aaaagagat cgagtttca 
      gcaaagccg caacgtgga attgagcga gaaagtgaa 
      gaagctaac gcatgaaat ttcccactt gcaacaaca 
      aatggtttt gtcccagaa ttcgtcgca acaatcaac 
      gcaactcct ccaagagaa tctaacaag ggaacagaa 
      gatccggct tatggccga ggatgagtg tcgggttaa 
      aaaccaaga gttgcagtt gaaattgca agaattggc 
      gcaacagta ccaagtcat ccaatcaaa atcggacga 
      aatcgccaa gttgcaaga cgccctcaa gtcgaaacc 
      cagcaccga ctttgagtc gcggattaa gtccctcac 
      tgatacgct catggtgaa gcaaaatgc cttagaacg 
      agttacaac cgaacgcaa cgcgttgcg aatccaact 
      agagaagct ggaaactga gtatcacaa taatttggc 
      gcagcttga gcgcgccca agtgcgtgt catcaacgt 
      gaatgatac agatgatgt taaatcgca agttccgca 
      atttatgcg cgtgtctcc gttcgatgc tggggtcac 
      aagacgggt caaacacgc gtattcgac tgttgatgc 
      gatcagtgt taggacggg gattttcct gcggcggta 
      ccccctggc cagggtttt tgttttctg ttatatggt 
      cttgttgca cttttgggc cttgattct actcttcct 
      gtatgcccc aagcagccg ttaataaac tttttaaaa 
      ttattttcg tatttcatt attacaggg caaagtgat 
      tctgattgt ccgtaaata ggtgattta attgcatat 
      tcaaattct gaaaaaatt aagaaacat tttctgtca 
      ttaaaaaaa ggcattgcc taaacgact gtcggcgct 
      gctgttagt ttacattaa taaaaagac cgtcgttcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY