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Gene information
General information
TC002339 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 207655..209484
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002339
    iBeetle screen iB_09558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002339 RA
      MTEPINQKE YLKKYLNMG KAPGEKKKK KKKKPTTSR 
      VQIIDDDAD ANIPTHVIE EDLALANED APQIVAIID 
      ERPPSMRVD EKTKNPLWQ PIGSVENSD TSEAKQSIK 
      SFKIAPKST DKLPFALPD KSPPRRKKK TTHGDNTPP 
      RRTPPREIL IKEENSPPR RSDTPPRPV IKEERRSEA 
      RSFDDNSPP RSRNGDRDI SPPRSRRDD DISPPRRRR 
      DDDISPPRR RKDDDISPP RRRRDDDIS PPSRRRNDD 
      ISPPRRRRD DISLPRKRR DDDTSLPRK RRDDDTSSP 
      RKRRNDGIS SPRKRNNDD ISPARKRSR DTSPSRRKG 
      PKDGDSPPR RHPNKTEER QSEKMKKTL DGKSAGLQN 
      ARELVEETK QFRSREDQM FKTLSKDVS GENAATVHR 
      NRKTGQIRN LEEEEEIQR KKREKEEEN KEKYTRWGR 
      GLVQVENRE QQIQEQLHE MSKPLARYA DDEDLERYL 
      KEQEREGDP MLAYIRKKK KKKAAEAGE VEKPLFEGN 
      FMPNRYGIR PGHRWDGVD RSNGYESKW FEVQNAKKA 
      ILEDSYKWS TEDM
    CDS sequence
    • >TC002339 RA
      atgaccgaa ccaataaat cagaaagaa tacctgaaa 
      aaatatctc aatatgggt aaagcccct ggtgaaaaa 
      aagaagaag aaaaagaaa aaaccgaca acgagcagg 
      gtccaaata atcgacgat gacgccgac gctaatatt 
      ccaacccat gtgattgag gaagacctt gcccttgct 
      aacgaagat gcgccccaa attgtggct ataattgac 
      gaaaggccc ccttcgatg cgcgttgat gaaaaaaca 
      aaaaatccc ttatggcaa cccatcgga agtgttgaa 
      aattcagac acttccgaa gcaaaacaa tcaatcaag 
      agtttcaaa attgcacca aaaagcacc gacaagttg 
      ccgtttgcg ttaccggat aagtcgccc cccagaaga 
      aagaagaaa acaactcac ggcgacaac acgcctcct 
      agacggaca cctccacgg gaaatcctc ataaaagag 
      gaaaattcg cccccaaga aggagcgac acaccgcca 
      agacctgtc atcaaggaa gaaagacga tcagaagct 
      cggagtttt gatgataat tcaccccca aggagcaga 
      aatggtgat cgtgacatt tcgcctccc aggagtagg 
      agagatgat gacatctcg cctccaaga agaaggaga 
      gatgatgac atttcccct ccaagaaga aggaaagat 
      gatgatatt tcccctcca agacgaagg agagacgat 
      gatatctcc cctccaagt agaaggaga aatgatgac 
      atttcccct ccaagaagg aggagggat gatatttcg 
      ttgcctagg aaacggagg gatgatgac acttcgttg 
      cctaggaaa cggagggat gatgacaca tcgtcacct 
      aggaaacga agaaatgat ggaatttct tctcccaga 
      aaaaggaac aatgatgat atttctcct gccagaaaa 
      agaagcaga gacacttcc ccatcgagg agaaaaggg 
      cctaaagat ggagattca cctccaaga aggcaccca 
      aataagact gaagaaaga cagtcggaa aaaatgaag 
      aaaactctg gatggaaaa tcggctggc cttcagaat 
      gctcgagaa ctggttgaa gaaacgaag caattccgt 
      tcccgcgaa gaccaaatg ttcaaaact ctgtctaaa 
      gatgtttcc ggtgagaac gcggcaaca gtccatcgc 
      aaccgaaaa accggtcag atccgcaac ttggaggaa 
      gaagaagaa atccaacga aagaaacgc gaaaaagag 
      gaagaaaac aaggagaaa tacacgcga tggggtcgt 
      gggttggtc caggtggaa aaccgggaa caacaaatc 
      caggaacaa ttgcacgaa atgagcaaa cctttggct 
      cgatatgct gacgatgaa gatttggag cgatatttg 
      aaggaacag gaacgtgag ggcgaccca atgttggcg 
      tacatcagg aaaaagaag aagaaaaag gctgcggaa 
      gcaggcgag gttgagaaa ccactgttc gaaggcaat 
      ttcatgccc aataggtac ggcataagg ccggggcat 
      aggtgggac ggggtcgat agatcaaac ggctacgag 
      agtaaatgg ttcgaggtg caaaacgcc aaaaaggcc 
      attctggaa gattcctac aagtggagt accgaagat 
      atgtag
    mRNA sequence
    • >TC002339 RA
      cctgtcatt caaaaatgt caattttgt aacctcaaa 
      aaatacgtt gtcaattga tacaacaaa atctaattt 
      gcgcaaaaa tgaccgaac caataaatc agaaagaat 
      acctgaaaa aatatctca atatgggta aagcccctg 
      gtgaaaaaa agaagaaga aaaagaaaa aaccgacaa 
      cgagcaggg tccaaataa tcgacgatg acgccgacg 
      ctaatattc caacccatg tgattgagg aagaccttg 
      cccttgcta acgaagatg cgccccaaa ttgtggcta 
      taattgacg aaaggcccc cttcgatgc gcgttgatg 
      aaaaaacaa aaaatccct tatggcaac ccatcggaa 
      gtgttgaaa attcagaca cttccgaag caaaacaat 
      caatcaaga gtttcaaaa ttgcaccaa aaagcaccg 
      acaagttgc cgtttgcgt taccggata agtcgcccc 
      ccagaagaa agaagaaaa caactcacg gcgacaaca 
      cgcctccta gacggacac ctccacggg aaatcctca 
      taaaagagg aaaattcgc ccccaagaa ggagcgaca 
      caccgccaa gacctgtca tcaaggaag aaagacgat 
      cagaagctc ggagttttg atgataatt cacccccaa 
      ggagcagaa atggtgatc gtgacattt cgcctccca 
      ggagtagga gagatgatg acatctcgc ctccaagaa 
      gaaggagag atgatgaca tttcccctc caagaagaa 
      ggaaagatg atgatattt cccctccaa gacgaagga 
      gagacgatg atatctccc ctccaagta gaaggagaa 
      atgatgaca tttcccctc caagaagga ggagggatg 
      atatttcgt tgcctagga aacggaggg atgatgaca 
      cttcgttgc ctaggaaac ggagggatg atgacacat 
      cgtcaccta ggaaacgaa gaaatgatg gaatttctt 
      ctcccagaa aaaggaaca atgatgata tttctcctg 
      ccagaaaaa gaagcagag acacttccc catcgagga 
      gaaaagggc ctaaagatg gagattcac ctccaagaa 
      ggcacccaa ataagactg aagaaagac agtcggaaa 
      aaatgaaga aaactctgg atggaaaat cggctggcc 
      ttcagaatg ctcgagaac tggttgaag aaacgaagc 
      aattccgtt cccgcgaag accaaatgt tcaaaactc 
      tgtctaaag atgtttccg gtgagaacg cggcaacag 
      tccatcgca accgaaaaa ccggtcaga tccgcaact 
      tggaggaag aagaagaaa tccaacgaa agaaacgcg 
      aaaaagagg aagaaaaca aggagaaat acacgcgat 
      ggggtcgtg ggttggtcc aggtggaaa accgggaac 
      aacaaatcc aggaacaat tgcacgaaa tgagcaaac 
      ctttggctc gatatgctg acgatgaag atttggagc 
      gatatttga aggaacagg aacgtgagg gcgacccaa 
      tgttggcgt acatcagga aaaagaaga agaaaaagg 
      ctgcggaag caggcgagg ttgagaaac cactgttcg 
      aaggcaatt tcatgccca ataggtacg gcataaggc 
      cggggcata ggtgggacg gggtcgata gatcaaacg 
      gctacgaga gtaaatggt tcgaggtgc aaaacgcca 
      aaaaggcca ttctggaag attcctaca agtggagta 
      ccgaagata tgtagtttt ttgtaaatt atgaataaa 
      acaaaaatt tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY