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Gene information
General information
TC002366 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 172196..173821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002366
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09557

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002366 RA
      MEENKNSGD ETPETEVTS NGENEAKVE SSEQPQDEA 
      PTEQKSEAT EPPRGETGQ DAASYDPKN VHYEGDLAV 
      YTDPKTGTQ YEWNSEKSE WRLREVKYS FEGDTHVYT 
      DKDGVKFFW DKEKKAWFP KVDDEFMAI YQLNYGFSE 
      NNSENSEEK KVDEEEPKV DKSQPKPKG EKRKASEPT 
      WFEVDETEN TKVYVSNLP TDIEEQEFV DLMQKCGLV 
      MKDPTNGSF KVKLYKEPG TGYLKGDAL CTYIKIESV 
      ELALSLLDG YDYKGHKLK VERAKFQMK GAYDPKLKP 
      KMKKRKEKL KLKKMQEKL FDWRPEKPI DERAKHEKV 
      VIVKNLFDP SIFDTDVGL ILEFQEDLR EEVSKIGEV 
      RKVMIYDRH PEGVAQINM SSPEEADEV VKLLNGRWF 
      MKRQLTAEI WDGKTKFKI AETDAQINE RLENWDKFL 
      EEDTPKNES VVNVDDLNK K
    CDS sequence
    • >TC002366 RA
      atggaggaa aataaaaat tccggggac gaaacacca 
      gagacggaa gtgactagt aatggcgaa aatgaggca 
      aaagtcgag tccagcgaa caaccacaa gatgaagct 
      ccaaccgag caaaaatca gaagcaaca gagcctcca 
      cgaggcgaa acgggtcaa gatgcggct tcatatgac 
      ccgaaaaac gtccactac gagggggat ctagctgtg 
      tatactgac cccaaaact ggtacgcaa tacgagtgg 
      aactctgag aaaagtgag tggcgtttg cgtgaagtt 
      aagtacagt ttcgaggga gatacacat gtctacact 
      gacaaagac ggtgttaaa ttcttctgg gacaaagag 
      aagaaggct tggtttccg aaagtggac gatgagttt 
      atggcaata tatcaattg aattacggt tttagcgaa 
      aataattcg gaaaattcg gaggagaag aaagtggac 
      gaggaagag ccaaaagtg gataaaagt cagcctaag 
      cccaaaggc gagaaacgc aaagcgtcc gaaccgacg 
      tggtttgag gtcgatgag acggaaaat actaaagtt 
      tacgtcagt aatttacca acggatatc gaggagcaa 
      gaattcgtt gatttgatg caaaagtgc ggacttgtg 
      atgaaagac cccactaat ggcagtttt aaagtaaaa 
      ttatacaaa gagccaggc actggttac ctcaaaggc 
      gacgccctt tgcacctac attaaaata gaatcagta 
      gagttagcc cttagttta ctggacggt tacgactac 
      aagggccac aagttgaag gtcgaacgc gccaagttc 
      cagatgaag ggcgcctac gaccccaaa ctcaagccc 
      aaaatgaag aaacgcaaa gagaaactc aaactcaaa 
      aaaatgcaa gagaaattg ttcgattgg cggccggaa 
      aaacccata gacgagcgc gccaagcat gagaaagtc 
      gttatcgtg aaaaacctc tttgatccg tcgattttc 
      gacacagac gtgggcctc atcctcgaa ttccaggag 
      gacctgcga gaggaagtt tccaaaatc ggggaagtc 
      cgcaaagtt atgatttac gacaggcat ccagaaggc 
      gtagcacag atcaacatg tcctcacct gaggaggcc 
      gacgaggtc gtgaaatta ctaaacgga cggtggttt 
      atgaaaaga cagttaact gcagagatt tgggacgga 
      aaaaccaag tttaagatt gcggaaacg gacgcacaa 
      attaacgaa aggttagag aattgggac aagtttttg 
      gaggaagac accccgaag aacgaatca gttgtaaac 
      gttgacgat ttaaataaa aaataa
    mRNA sequence
    • >TC002366 RA
      ctctttaat taacctaaa atttatttt ccaaaatat 
      tttttatat taataatta aacaattaa tggtgcatg 
      tatactccc cacaatgga ggaaaataa aaattccgg 
      ggacgaaac accagagac ggaagtgac tagtaatgg 
      cgaaaatga ggcaaaagt cgagtccag cgaacaacc 
      acaagatga agctccaac cgagcaaaa atcagaagc 
      aacagagcc tccacgagg cgaaacggg tcaagatgc 
      ggcttcata tgacccgaa aaacgtcca ctacgaggg 
      ggatctagc tgtgtatac tgaccccaa aactggtac 
      gcaatacga gtggaactc tgagaaaag tgagtggcg 
      tttgcgtga agttaagta cagtttcga gggagatac 
      acatgtcta cactgacaa agacggtgt taaattctt 
      ctgggacaa agagaagaa ggcttggtt tccgaaagt 
      ggacgatga gtttatggc aatatatca attgaatta 
      cggttttag cgaaaataa ttcggaaaa ttcggagga 
      gaagaaagt ggacgagga agagccaaa agtggataa 
      aagtcagcc taagcccaa aggcgagaa acgcaaagc 
      gtccgaacc gacgtggtt tgaggtcga tgagacgga 
      aaatactaa agtttacgt cagtaattt accaacgga 
      tatcgagga gcaagaatt cgttgattt gatgcaaaa 
      gtgcggact tgtgatgaa agaccccac taatggcag 
      ttttaaagt aaaattata caaagagcc aggcactgg 
      ttacctcaa aggcgacgc cctttgcac ctacattaa 
      aatagaatc agtagagtt agcccttag tttactgga 
      cggttacga ctacaaggg ccacaagtt gaaggtcga 
      acgcgccaa gttccagat gaagggcgc ctacgaccc 
      caaactcaa gcccaaaat gaagaaacg caaagagaa 
      actcaaact caaaaaaat gcaagagaa attgttcga 
      ttggcggcc ggaaaaacc catagacga gcgcgccaa 
      gcatgagaa agtcgttat cgtgaaaaa cctctttga 
      tccgtcgat tttcgacac agacgtggg cctcatcct 
      cgaattcca ggaggacct gcgagagga agtttccaa 
      aatcgggga agtccgcaa agttatgat ttacgacag 
      gcatccaga aggcgtagc acagatcaa catgtcctc 
      acctgagga ggccgacga ggtcgtgaa attactaaa 
      cggacggtg gtttatgaa aagacagtt aactgcaga 
      gatttggga cggaaaaac caagtttaa gattgcgga 
      aacggacgc acaaattaa cgaaaggtt agagaattg 
      ggacaagtt tttggagga agacacccc gaagaacga 
      atcagttgt aaacgttga cgatttaaa taaaaaata 
      agttggaat ttggcgcgt ttcattcgg tgtgtacag 
      tgtgctgca ttttggatg tttgaactt tgaatacaa 
      gatactcat ttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY