Status
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Gene information
General information
TC006851 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450974.1: 94347..149299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006851
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09553

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006851 RA
      MGSRIRENV KHFFECFCE VVPPTGDKA AWIIQQYPD 
      KYKDEEVLK AVPNFAYPY KFENTVIQH YSFVLTDLE 
      SKWTFGFCR HDPRGETAI VVLSYLPWH QAFYKFLDN 
      IAVLMTSPR SEDLSEFLS AVYNTKLAE AGKYFTVPF 
      NRGESNFSV EVPKPFQLP SIPENRNLT EYYNAVDSH 
      NMMIIFASM LYERRIIFT SKKLKRLSA CVQSANDVI 
      YPMIWQHIF IPVLPMALI DYLLAPMPF LIGVPEEVM 
      KKVQRHEIG EVVILDADA NTVTTPFDD LNNLPQEVI 
      TSLKRQLKN KSALLGDGV SRAFLRALV QLIGGYRDG 
      LRIQEAQKI TFDQEKFIE TRPVSFQPF LREMLHLQI 
      FQQFIEERL AMLNNGLGF SDEFEMETF RYSVQSGSK 
      LKQQYKDWT STMKKEGSA FFKSVKEKA NPAVKSAVK 
      TVKDGGKGV KSAYRGLRS RLKENQGPQ QLQNGLDSP 
      SDKPRSAPN SPTGKRRTF AGISVPTTN HRKDTALSF 
      KSSGFERSY SPLSPTSQP MSPDGLDNS RSGSPNLEF 
      PRIDLMNEM KDLLQLRLD TPPVDRSLK PVRSLENFK 
      QHTSPTSHS PDRWVFPSR TGAPMASPP KRFFLPNQP 
      SSETQNLLL QSPDDVFSS PPVPPRVAA KDLFGTTPF 
      VVAGGHSEN GDPLFLVAN QHSIDIVKL SPPTCSPRR 
      KPEDMEDLI RLDSTSDTD DFDPLKTNS YSSSAPLSL 
      SNPLYTYEN HTTKQNGSV QNRNDQDLL QEYGLDFSF 
      TSAPNQQIF DPFAGTSAS NKVNSQSQW TRFD
    CDS sequence
    • >TC006851 RA
      atgggttcc aggatacgc gagaacgtc aagcacttt 
      ttcgaatgc ttctgcgag gtggtgccc cccacgggg 
      gacaaagcc gcctggatc atccagcag taccccgac 
      aaatacaaa gacgaggaa gtgctcaaa gctgtacca 
      aattttgca tatccttac aaattcgaa aacactgtt 
      atccagcat tattccttc gtcctgacg gatttggag 
      tcgaagtgg acgttcggt ttttgcaga catgatccg 
      agaggagag accgcgatt gtggtcttg agttatcta 
      ccctggcat caggcgttt tataagttt ctagataac 
      atagcagta ttaatgacc agtccgcga tccgaagac 
      ttgagcgaa ttcttgtcg gccgtttac aacacgaaa 
      ttagcggaa gcgggtaaa tatttcact gttcctttc 
      aaccgagga gagagtaac ttctctgtt gaggtccct 
      aaaccgttc cagttgccg agtataccg gaaaaccgc 
      aacctcacc gagtattac aacgctgtg gattcgcac 
      aacatgatg ataatcttc gcgtcaatg ttgtacgaa 
      aggaggatc atcttcacg tcgaagaaa ctgaaaaga 
      ttgagcgct tgtgtgcaa tcggccaac gatgtgatt 
      tatccaatg atatggcag cacattttc attccggtg 
      cttccgatg gcactgata gattatttg ctggcgccg 
      atgcctttc cttattggt gtacctgag gaagtaatg 
      aagaaagtg cagagacac gaaatcggc gaagttgtg 
      attctggac gcggacgca aacaccgtc acgacgcca 
      ttcgacgac ctcaataat ctacctcaa gaagtgatc 
      acgtcgcta aaacgacaa ttgaaaaat aagtcggca 
      ctattgggt gatggtgta tcgcgagcg tttcttcgg 
      gctcttgtc caattaatt ggaggatat cgggacgga 
      cttaggata caagaagcg cagaaaatc accttcgat 
      caggagaag ttcatcgag acaagacct gtgtcgttt 
      cagcccttc ctgagggag atgctgcat ctgcaaatc 
      ttccaacaa tttattgag gaacgactt gcgatgttg 
      aacaacggc ctcggtttt tccgacgaa ttcgaaatg 
      gaaacattc cgctactct gtccaatcc ggctcgaaa 
      ctgaagcaa caatacaaa gactggact tcaacaatg 
      aaaaaggaa ggttcagcc tttttcaaa agtgtcaaa 
      gaaaaggcc aatccggct gttaagtcc gctgtcaaa 
      acggtaaaa gacggtggc aagggtgta aaaagtgcc 
      taccgaggt ctgcgttcc cgtctgaaa gaaaaccaa 
      ggaccgcaa caactccaa aacggcctc gacagtcct 
      agtgataag ccgcgatca gcaccgaat tcgccgacc 
      ggaaaacgg cggacattc gccggcatc agcgtgcct 
      acaacaaac caccggaaa gatacggcg ttgtctttc 
      aaaagttca ggctttgaa aggtcgtac agtccgctg 
      agtccgacg tctcaaccc atgtctcca gacggtttg 
      gacaattca cgtagcggg agccccaat ttggagttc 
      cctcgtatc gacttgatg aatgagatg aaggattta 
      ttgcagcta cgcttggac accccacca gtggacaga 
      tctctaaaa ccggtccgt agcttggaa aacttcaaa 
      cagcacaca tccccgacg tcacacagc cccgaccgt 
      tgggtgttt ccgtcgcgt accggcgcc cccatggcc 
      tcgcccccc aaacgcttc ttcctcccc aatcaaccc 
      tcctccgaa acccaaaac ctcctcctc caatccccc 
      gacgatgtt ttttcctcc ccgcccgtt ccaccacgc 
      gttgcggcc aaagatctg ttcggaacg acgccgttt 
      gtggtcgca ggggggcac agcgaaaac ggtgatccc 
      ctttttctt gtcgctaat caacactct attgatatc 
      gttaagcta tcaccgccc acttgttcg cctaggcgg 
      aagcccgaa gacatggag gacttgatc cgactggac 
      tcaacatcg gacaccgac gacttcgat ccactcaaa 
      acgaactca tacagcagc agcgccccc ttgagtctt 
      tcaaacccg ctctatacg tacgaaaat cacacgaca 
      aaacagaac ggttcagtg caaaacaga aacgatcag 
      gacttactc caagagtac ggtctagac tttagtttc 
      accagtgcg ccaaaccaa caaattttc gatccgttc 
      gctggcact agtgcaagt aacaaagtc aattctcaa 
      agtcagtgg actagattc gactga
    mRNA sequence
    • >TC006851 RA
      taaaatttt caagttcaa cttggaaca aatcacaat 
      aacctttgt tagcaaaat taaaaaaag tgcgattaa 
      caattaaag attgggtaa agatccctt ttttgtcag 
      ctgtttcca aggttggtg ctcctgctg tgcgcttcc 
      atcttcgtc tcacccact agatttcgt tatttccat 
      tcagtctgt cagtgtccg tgtcaatcg gtggttgtt 
      ttcgtgccc ccttggagc aatatgtgt taaaattgg 
      cgctatgcc tccaaaaat ctccagttc tatcgcccc 
      cacaatgga ttactaaca ccaccagca tgggttcca 
      ggatacgcg agaacgtca agcactttt tcgaatgct 
      tctgcgagg tggtgcccc ccacggggg acaaagccg 
      cctggatca tccagcagt accccgaca aatacaaag 
      acgaggaag tgctcaaag ctgtaccaa attttgcat 
      atccttaca aattcgaaa acactgtta tccagcatt 
      attccttcg tcctgacgg atttggagt cgaagtgga 
      cgttcggtt tttgcagac atgatccga gaggagaga 
      ccgcgattg tggtcttga gttatctac cctggcatc 
      aggcgtttt ataagtttc tagataaca tagcagtat 
      taatgacca gtccgcgat ccgaagact tgagcgaat 
      tcttgtcgg ccgtttaca acacgaaat tagcggaag 
      cgggtaaat atttcactg ttcctttca accgaggag 
      agagtaact tctctgttg aggtcccta aaccgttcc 
      agttgccga gtataccgg aaaaccgca acctcaccg 
      agtattaca acgctgtgg attcgcaca acatgatga 
      taatcttcg cgtcaatgt tgtacgaaa ggaggatca 
      tcttcacgt cgaagaaac tgaaaagat tgagcgctt 
      gtgtgcaat cggccaacg atgtgattt atccaatga 
      tatggcagc acattttca ttccggtgc ttccgatgg 
      cactgatag attatttgc tggcgccga tgcctttcc 
      ttattggtg tacctgagg aagtaatga agaaagtgc 
      agagacacg aaatcggcg aagttgtga ttctggacg 
      cggacgcaa acaccgtca cgacgccat tcgacgacc 
      tcaataatc tacctcaag aagtgatca cgtcgctaa 
      aacgacaat tgaaaaata agtcggcac tattgggtg 
      atggtgtat cgcgagcgt ttcttcggg ctcttgtcc 
      aattaattg gaggatatc gggacggac ttaggatac 
      aagaagcgc agaaaatca ccttcgatc aggagaagt 
      tcatcgaga caagacctg tgtcgtttc agcccttcc 
      tgagggaga tgctgcatc tgcaaatct tccaacaat 
      ttattgagg aacgacttg cgatgttga acaacggcc 
      tcggttttt ccgacgaat tcgaaatgg aaacattcc 
      gctactctg tccaatccg gctcgaaac tgaagcaac 
      aatacaaag actggactt caacaatga aaaaggaag 
      gttcagcct ttttcaaaa gtgtcaaag aaaaggcca 
      atccggctg ttaagtccg ctgtcaaaa cggtaaaag 
      acggtggca agggtgtaa aaagtgcct accgaggtc 
      tgcgttccc gtctgaaag aaaaccaag gaccgcaac 
      aactccaaa acggcctcg acagtccta gtgataagc 
      cgcgatcag caccgaatt cgccgaccg gaaaacggc 
      ggacattcg ccggcatca gcgtgccta caacaaacc 
      accggaaag atacggcgt tgtctttca aaagttcag 
      gctttgaaa ggtcgtaca gtccgctga gtccgacgt 
      ctcaaccca tgtctccag acggtttgg acaattcac 
      gtagcggga gccccaatt tggagttcc ctcgtatcg 
      acttgatga atgagatga aggatttat tgcagctac 
      gcttggaca ccccaccag tggacagat ctctaaaac 
      cggtccgta gcttggaaa acttcaaac agcacacat 
      ccccgacgt cacacagcc ccgaccgtt gggtgtttc 
      cgtcgcgta ccggcgccc ccatggcct cgcccccca 
      aacgcttct tcctcccca atcaaccct cctccgaaa 
      cccaaaacc tcctcctcc aatcccccg acgatgttt 
      tttcctccc cgcccgttc caccacgcg ttgcggcca 
      aagatctgt tcggaacga cgccgtttg tggtcgcag 
      gggggcaca gcgaaaacg gtgatcccc tttttcttg 
      tcgctaatc aacactcta ttgatatcg ttaagctat 
      caccgccca cttgttcgc ctaggcgga agcccgaag 
      acatggagg acttgatcc gactggact caacatcgg 
      acaccgacg acttcgatc cactcaaaa cgaactcat 
      acagcagca gcgccccct tgagtcttt caaacccgc 
      tctatacgt acgaaaatc acacgacaa aacagaacg 
      gttcagtgc aaaacagaa acgatcagg acttactcc 
      aagagtacg gtctagact ttagtttca ccagtgcgc 
      caaaccaac aaattttcg atccgttcg ctggcacta 
      gtgcaagta acaaagtca attctcaaa gtcagtgga 
      ctagattcg actgaaaag tgatattta gcaagagtt 
      ttatacggt ttttatgaa tgtgcaaat gtgttttta 
      atgcaaatt ttatttata cacgagtta tgtattata 
      tgttttggg cggatacaa gttacgtaa tttatgtta 
      ttttgttgt ttagtacaa tttttccta ggtaagagc 
      aaccgaaac atacctaaa gaagactcg gaaattcta 
      gaattagct gtgatactc tcgattgtg tttgattgg 
      aagaaatcg gtttatttt tgttaaatt gcacgaatc 
      catcataga ccaacaact tataaaatt gagaaagtt 
      tattacgtc tgaacttaa aataggcta agctataat 
      actgtgatt gtaacactt gtatattca cctgattaa 
      attgtaaat gcctcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY