Status
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Gene information
General information
TC012903 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 55871..63252
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012903
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09552

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012903 RA
      MLKKALVLS IFISYVCCQ RFNEFHFVD PFSESWNWH 
      SRHNLFPRE REVEEITER DREQLSPNY QDPATSGTE 
      EKPVLKPKP QKPSSSYNP SVIPGGVVD IDAVGSPQL 
      PTFPEADFG FSSFGGGAH SPVGGGLFG LGGFFGSVS 
      DLKPWWKGP NVCIEREES ADDDEEEED PLESKKNET 
      ESETEKVPN FFSTAISLS NCRQSANKY ECVTKINNH 
      GVVKTFTVR YKCCYGFVR TKGGSGCEK QLELKPLLE 
      TINDLDAKV FRNLIQSTG LEETFKNGN YTIFVPTDT 
      ALNEFNEKM LELNNVDLR GDAKNALSN NDLVLSHAV 
      AGFIDLTDN SNEDIIYSE NKNSSIRIN TYPTQNHEK 
      MVTVNCAKI TKPNNLASN GIAYLIDGV ATPATQTIE 
      DIIKEHKKL SNFRKVLES TDSFKNLRV NGHYTIFAP 
      TDEAFNKLE PAAKQKLLR GDACAVSIL KHHVTAHTV 
      CSAAIMGNA TTHNIEGEI LNLERTSDD QLILEEKAK 
      LIETDIMAI NGVIHLIDT VLIPDSALH INAALKNEN 
      LTKFRELIE QAGFVEDID NLNNATVFA PSNEAFDDP 
      KVVKLLNEI KGDKEKLRD LILYHTLQG QLQSCDMNN 
      NALLKTNDH DKELRLNLY STLPLFSNV INRGTINCA 
      RIVGFDEKS CGSVIHEVS KVLLPPTQT ILDLINADS 
      KYSTLSGII KGTEVEKIL QENNRSITF LAPTDETLA 
      AIDEADLKT LKEDKEKAT TVLKNHILT EVLCCSGVG 
      PQSWGFNTL VPTMTRQHH QVGRTGSHQ IRIGRAVVT 
      SCDNLATNG VLHTVNKVL FPQRPTSPS FGGFFLFDF
    CDS sequence
    • >TC012903 RA
      atgctgaag aaagcatta gtcttgagc atcttcatc 
      tcctacgtt tgttgccag cgattcaac gaatttcat 
      tttgttgac cctttctcc gagtcatgg aactggcac 
      agtcgccac aatttattt cctcgcgaa agagaagtt 
      gaggaaatc accgaacga gaccgtgag caactgagt 
      cccaattac caggaccct gccacttct ggcaccgag 
      gagaagccc gttctcaag ccaaaaccc caaaaaccc 
      tcctcctcc tacaaccct tctgttatt cccggtggt 
      gttgtggac atcgatgcc gtcggaagt cctcaactc 
      ccaaccttt ccggaggcc gacttcggc ttttcctcg 
      ttcggaggc ggagctcac tcacctgtt gggggtggt 
      ctttttgga cttgggggt ttcttcggt tcggtctct 
      gacttgaag ccgtggtgg aaaggtcct aacgtgtgt 
      atcgaacgt gaagaatca gccgatgat gacgaagag 
      gaggaagac cctctcgaa tcgaaaaag aacgaaacc 
      gaaagtgaa accgaaaaa gtccccaac tttttctca 
      acagccatc agcttgtcc aactgccgt caatcggct 
      aacaaatac gaatgtgtt acaaaaatt aacaatcat 
      ggtgtcgtg aaaaccttc acggtccgt tacaagtgt 
      tgttatggt tttgtgcgt acgaaaggg ggctcagga 
      tgtgaaaaa caactcgaa ttgaaacct ctcctagag 
      accatcaat gatttggac gccaaagtc ttccgcaat 
      ttgattcaa agcaccggc ctggaggag actttcaaa 
      aatggcaac tacacgatc tttgtgccc actgacacg 
      gctctcaac gagttcaac gagaaaatg ctagaactg 
      aataacgtg gacttgcga ggtgacgca aagaacgct 
      ctctcgaac aacgatctc gtcctcagt catgccgtt 
      gccggtttc atcgaccta acggacaat tccaatgaa 
      gacataatt tacagcgaa aataaaaac agctcgatc 
      aggatcaac acgtatccg actcagaat cacgagaag 
      atggtcacg gtcaattgc gcaaaaatc acaaagccg 
      aacaatctc gctagtaac ggaatcgca tatctaatc 
      gatggagtt gccacaccg gccacacaa accattgaa 
      gatatcatt aaagaacac aagaaactg tccaacttc 
      cgaaaagta cttgaaagc accgattct ttcaaaaat 
      ttgcgagtg aatggccat tatacgatt ttcgcgcca 
      accgatgaa gcctttaac aaacttgag cctgcagct 
      aagcaaaag ttgctccga ggagatgct tgcgctgtt 
      agcattctc aagcaccac gttacagct cacactgtg 
      tgttctgcg gccattatg ggcaatgct accacacat 
      aatatcgaa ggcgaaatt ctcaacttg gagaggaca 
      tccgatgac cagcttatt ctggaggag aaggccaaa 
      ctgatcgaa actgacatc atggctatt aatggtgtt 
      atccatttg attgatact gttttaatc cctgattca 
      gccctccat atcaatgca gctttgaag aatgagaat 
      ttgaccaaa ttcagggaa ttgatcgaa caagccggt 
      tttgttgaa gatatcgat aatttgaac aatgctaca 
      gtttttgct ccatcaaat gaggctttt gatgacccc 
      aaagtcgtc aaattgtta aacgagatc aagggagat 
      aaagagaaa ttgagggat ttgattctg tatcacact 
      cttcaagga cagttacaa tcctgcgat atgaacaat 
      aatgccctc ttaaaaaca aacgaccat gataaggaa 
      cttcgactc aatttgtat tcaacgttg ccgttgttt 
      agtaacgtt ataaaccga gggaccatt aactgcgct 
      agaatcgtc ggtttcgac gaaaaatcg tgcggctcg 
      gtgatccat gaagtcagc aaggtcctt ctacctcca 
      acccaaacc attcttgac ctgattaac gccgattcg 
      aaatatagc actttgagt ggaataatc aaaggaacg 
      gaagttgag aaaatcctg caggaaaac aatcgttca 
      atcaccttc ttggcaccc actgacgag actctcgct 
      gcaatcgac gaagccgat ttgaagaca ctcaaagag 
      gacaaggag aaggccaca acggtgttg aaaaaccac 
      attttaacg gaagtttta tgttgttcg ggagtgggg 
      ccacaatcg tggggtttc aatactttg gtccctact 
      atgaccaga cagcaccat caagttggt aggactgga 
      agtcatcaa ataaggatt ggacgggct gttgttaca 
      agctgtgat aatttagct acaaatggt gtgctacac 
      actgtgaat aaagtatta ttcccccaa aggccgact 
      tcgcccagt tttggaggg tttttcttg tttgatttc 
      taa
    mRNA sequence
    • >TC012903 RA
      tgaattatt attccaccg atattgatg ttatcttgc 
      agaaaatat gttcagatc acacatagc cgcctaaac 
      atcaccaaa ggaatttaa atcgagcat taattaaaa 
      caaaaaggg cgccatagt atttctaat tatctccta 
      attaaatac aggattccc cacttgcct ctcattgct 
      aaatttaat agtagtatc tgttaattt ttagcataa 
      aaacctcta cctaaattt ggcctgaag cactcgagg 
      tgataatca aaccaaatt aactgttgt aatttcctg 
      cacttgttt atacaaaaa agatattta caactggat 
      tgaaataaa atgcgtatt attttgtga cgtactaaa 
      gtcatttcg aggctattt cagaattaa agtccgaaa 
      gcacaaaca taaggtgaa aatttattt tttacaagt 
      gggtttgtt agatattat tattaatgc aagtggaac 
      gttttcttg agcattacc tactatcac tataatcaa 
      atagatatt atttttact ttaaatgtg aatcatatg 
      aaattaaaa aatattaat ttacaatct ctgaccttt 
      taagcgata tttgatatt taacaaata aactcttcc 
      ttgttgtac ctactataa aatctcatc ccgtgtttt 
      tccctcgat ttttccata gagatccaa cacttttta 
      ttttgtgcc acatgttta tttctataa tatgattga 
      taactttct atcgttcta gaaagttgt gtaacaaat 
      ctttgaaga cagaaataa acaagactg taatagatt 
      tttctggac taaatctgc ccattatct aatcgtgcg 
      aattaactc ggatagggt ttttaaaga ggaagatgt 
      ataacgaat ggaaacgtt tatgcgcct caattataa 
      taattgtgt ttatcggca tttccacgc aaaactgta 
      atttaaact gaaagaaaa ataatggtt tgttctttt 
      tcttcttaa ttacacatg ttaacgact gttggaata 
      actagcggt tccaaagaa ccactagct gaccatcca 
      tccatctat cagggaaaa ttccagaat gtaaattta 
      ttttatcta tgttgctgg acatttctc tattcttag 
      aacgcgtat gaataggaa gatggtcga ctttcatcg 
      ttccactat ccatggacc catctcacc aatccagta 
      atgcttttc tctggtaca gatgaagat aaataaaat 
      gaaacaaag ttgacctaa cggtcgtta tcattggtc 
      ctgttgatg agcacccac tccccacca gcacctacg 
      acaacgttt atatacagc accatgccg aagaaacac 
      tccagtcaa ttgtaaaat aacgcagag tggatttag 
      caagaaaat tgtgttgca agagtgatt tcacaacga 
      cttgtcacg atgctgaag aaagcatta gtcttgagc 
      atcttcatc tcctacgtt tgttgccag cgattcaac 
      gaatttcat tttgttgac cctttctcc gagtcatgg 
      aactggcac agtcgccac aatttattt cctcgcgaa 
      agagaagtt gaggaaatc accgaacga gaccgtgag 
      caactgagt cccaattac caggaccct gccacttct 
      ggcaccgag gagaagccc gttctcaag ccaaaaccc 
      caaaaaccc tcctcctcc tacaaccct tctgttatt 
      cccggtggt gttgtggac atcgatgcc gtcggaagt 
      cctcaactc ccaaccttt ccggaggcc gacttcggc 
      ttttcctcg ttcggaggc ggagctcac tcacctgtt 
      gggggtggt ctttttgga cttgggggt ttcttcggt 
      tcggtctct gacttgaag ccgtggtgg aaaggtcct 
      aacgtgtgt atcgaacgt gaagaatca gccgatgat 
      gacgaagag gaggaagac cctctcgaa tcgaaaaag 
      aacgaaacc gaaagtgaa accgaaaaa gtccccaac 
      tttttctca acagccatc agcttgtcc aactgccgt 
      caatcggct aacaaatac gaatgtgtt acaaaaatt 
      aacaatcat ggtgtcgtg aaaaccttc acggtccgt 
      tacaagtgt tgttatggt tttgtgcgt acgaaaggg 
      ggctcagga tgtgaaaaa caactcgaa ttgaaacct 
      ctcctagag accatcaat gatttggac gccaaagtc 
      ttccgcaat ttgattcaa agcaccggc ctggaggag 
      actttcaaa aatggcaac tacacgatc tttgtgccc 
      actgacacg gctctcaac gagttcaac gagaaaatg 
      ctagaactg aataacgtg gacttgcga ggtgacgca 
      aagaacgct ctctcgaac aacgatctc gtcctcagt 
      catgccgtt gccggtttc atcgaccta acggacaat 
      tccaatgaa gacataatt tacagcgaa aataaaaac 
      agctcgatc aggatcaac acgtatccg actcagaat 
      cacgagaag atggtcacg gtcaattgc gcaaaaatc 
      acaaagccg aacaatctc gctagtaac ggaatcgca 
      tatctaatc gatggagtt gccacaccg gccacacaa 
      accattgaa gatatcatt aaagaacac aagaaactg 
      tccaacttc cgaaaagta cttgaaagc accgattct 
      ttcaaaaat ttgcgagtg aatggccat tatacgatt 
      ttcgcgcca accgatgaa gcctttaac aaacttgag 
      cctgcagct aagcaaaag ttgctccga ggagatgct 
      tgcgctgtt agcattctc aagcaccac gttacagct 
      cacactgtg tgttctgcg gccattatg ggcaatgct 
      accacacat aatatcgaa ggcgaaatt ctcaacttg 
      gagaggaca tccgatgac cagcttatt ctggaggag 
      aaggccaaa ctgatcgaa actgacatc atggctatt 
      aatggtgtt atccatttg attgatact gttttaatc 
      cctgattca gccctccat atcaatgca gctttgaag 
      aatgagaat ttgaccaaa ttcagggaa ttgatcgaa 
      caagccggt tttgttgaa gatatcgat aatttgaac 
      aatgctaca gtttttgct ccatcaaat gaggctttt 
      gatgacccc aaagtcgtc aaattgtta aacgagatc 
      aagggagat aaagagaaa ttgagggat ttgattctg 
      tatcacact cttcaagga cagttacaa tcctgcgat 
      atgaacaat aatgccctc ttaaaaaca aacgaccat 
      gataaggaa cttcgactc aatttgtat tcaacgttg 
      ccgttgttt agtaacgtt ataaaccga gggaccatt 
      aactgcgct agaatcgtc ggtttcgac gaaaaatcg 
      tgcggctcg gtgatccat gaagtcagc aaggtcctt 
      ctacctcca acccaaacc attcttgac ctgattaac 
      gccgattcg aaatatagc actttgagt ggaataatc 
      aaaggaacg gaagttgag aaaatcctg caggaaaac 
      aatcgttca atcaccttc ttggcaccc actgacgag 
      actctcgct gcaatcgac gaagccgat ttgaagaca 
      ctcaaagag gacaaggag aaggccaca acggtgttg 
      aaaaaccac attttaacg gaagtttta tgttgttcg 
      ggagtgggg ccacaatcg tggggtttc aatactttg 
      gtccctact atgaccaga cagcaccat caagttggt 
      aggactgga agtcatcaa ataaggatt ggacgggct 
      gttgttaca agctgtgat aatttagct acaaatggt 
      gtgctacac actgtgaat aaagtatta ttcccccaa 
      aggccgact tcgcccagt tttggaggg tttttcttg 
      tttgatttc taatgtatt ccagagtga tattttaat 
      taggtacgg actgagaaa aatgtactt ttcaatcat 
      ctttacaaa cgcacttat ttataataa tttataaca 
      ggaattcga gttgtagca acgtttata aataaaact 
      tttagaaaa ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY