Status
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Gene information
General information
TC012909 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 37515..43469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012909
      iBeetle screen iB_09551

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012909 RA
        MNSPPLAPQ DDVIQNISY TDSDTEYSA KIVGHEVTV 
        QCNSGQHNH GSSKVKLIN RIDYNWEFH YYRGQLVAV 
        HINGKIIAY GMKGKDGGM IRVTNQETN ERALIKNLK 
        NDVKDLSFA FSRQQIILG CIDDEGNVF IYEIIDEPS 
        AIKYKQLLH IFHSDVVPH SSPNFRIIW CPYLTCFDE 
        EEETPDDPE KMFVVLNWD KAEIWNFGM INAKYGPGP 
        LQPNENYEG YVEISHTAE LVDASFSSD GTAIAIACL 
        DGYVKFFQI YMVDNEKQK CLHEWRPHD GKPLSSIIF 
        VDNLLECSS QCWKFTITG ANHNSELKL WSCETWTCL 
        QTIHFKPDP SNLIQSVYL KMSIDYSGQ YLLMSDLNN 
        KVLYVLQLK RSDEAQQIS VTMISQFLL PTAFLSFHI 
        TEANCRKLR SSDMSEEFS QDADQDDDD EDDTVAAVV 
        IKMLVIQQK KFQECNIVF QPDAMLCNT EIIFNEIKE 
        ELEKVPKLD DLQSSVTLL IKQQQQQQL TLMTPDAFT 
        TAEQNSRPN SVRTSINGD INKSTEGTN GDTVENLID 
        FQRPEKDNF ASGGSSPSR EVQEILSLN NSTYSTQDY 
        FDNLTKMQE EQEIPQKEY KHNENLMYQ ENPNPEMVW 
        LKLPLVKKD NEINKEDNL RLGGGDEIT NLNETEWDK 
        NQWQAINFR LTSIENAIS DQNVIIQKI HQELKSLNQ 
        TKQGLSEDF NKELDLTMS KQQLQIAKM LENLINMQK 
        NNDRDQQDH VIASVSQIV VKSVSDKLQ NIVAHEMKH 
        VVVPTIRNL VETLKHQLD VQNSQKLNS DMVLQDKIS 
        KIINSKALA ESLSLSVAN VITPILEKC YRDMITSTL 
        VPSWEKVCG NMFHQINET FTQGTKEYT ASVEHYMER 
        QRRVQERGK DLIVQMQSV SENLQMNTD KLVGTLSSE 
        IHKQFNTSM KHTQENLLQ SMKDLISEQ IKLGFKSHA 
        SFLEDSVIN AVRSRAVTP SPHVESQVT LAHLDHLLA 
        KESYDEAFQ LALSAENLN YVIYVCEKV DSNILFHGS 
        SPPPQNCIL ALIQQLSME LHKNTEIKL SYIRAALVA 
        LIPGYPHTR QFIPKVLKD LLKQLQLFM TTNPSSKFI 
        TEAKLLKMA VESILDK
      CDS sequence
      • >TC012909 RA
        atgaattcg ccaccgctc gcccctcaa gacgacgtg 
        atccaaaac atcagttac accgattct gatacggaa 
        tattctgca aaaattgtg ggtcatgaa gtaacagtt 
        cagtgtaac tcgggacag cataaccat ggaagttct 
        aaagtgaaa cttatcaat cggattgat tataattgg 
        gagtttcac tattatcgc ggacaattg gtcgctgtt 
        cacattaat ggaaaaatt attgcttat gggatgaag 
        gggaaagac gggggcatg atccgagtt acgaaccaa 
        gagaccaac gaaagagca ctaatcaaa aacttgaaa 
        aatgatgtc aaagacctg tcttttgct ttttcacgc 
        caacagatt attttagga tgtattgac gacgaaggc 
        aatgttttc atttatgaa atcattgat gaacctagt 
        gcaataaaa tacaaacaa ctactccac atattccac 
        agtgatgtg gtcccacat tcaagcccc aactttcga 
        attatctgg tgtccctac ttgacctgt tttgacgag 
        gaagaggag acaccagat gatcctgaa aaaatgttt 
        gtggtatta aattgggac aaagccgaa atatggaat 
        tttggcatg attaatgca aagtatggg ccaggtcct 
        ttacaacca aatgaaaac tatgagggt tatgttgaa 
        attagccat actgctgaa ttggtagat gcttcattt 
        tcgtcagat gggaccgct attgcaatt gcttgctta 
        gacggttat gtgaaattt tttcaaatt tacatggtt 
        gacaatgaa aagcaaaag tgcttgcat gagtggaga 
        ccacatgat ggtaaacca ctatcttct atcatattt 
        gtcgacaat ttgctggag tgcagttct cagtgttgg 
        aaattcaca ataacgggt gcgaatcac aactctgag 
        ctaaagttg tggtcttgt gaaacgtgg acttgtctc 
        cagacaatc catttcaaa ccagatcca tcgaatcta 
        atccaaagt gtttatctt aaaatgagt atagactac 
        tcgggtcag tatttatta atgtccgat ctaaacaat 
        aaagtttta tacgtcctg caattaaaa cgaagcgat 
        gaagctcaa caaatatca gtaacaatg atatcgcaa 
        tttttacta ccaactgcc tttttgagc ttccacata 
        accgaagcc aactgtcgc aaattacgt tcgtccgat 
        atgagtgaa gagttttca caagatgcc gatcaagat 
        gacgatgat gaagacgat actgttgca gctgttgtt 
        atcaaaatg ttggtgatt cagcagaaa aaattccaa 
        gagtgtaat attgtattt caaccagat gcgatgttg 
        tgtaatact gagattata tttaatgaa attaaagag 
        gaattggag aaagtgcca aaattggac gatcttcag 
        tcatccgtt actttatta ataaaacaa cagcaacag 
        caacaatta actttgatg actcctgac gcttttaca 
        acagctgaa caaaactct cgtcctaat agtgtcaga 
        actagcatc aatggtgat attaataaa tcaaccgaa 
        ggcacaaat ggcgatact gttgagaat ttgattgat 
        tttcaaaga cctgaaaag gataatttt gcaagtgga 
        ggttctagt cctagtaga gaagttcag gaaattcta 
        tctcttaac aattcaacg tacagcact caggactat 
        ttcgataat ttaacgaaa atgcaagaa gaacaagaa 
        ataccccag aaggagtac aaacataac gaaaatctc 
        atgtaccaa gagaacccc aatcctgaa atggtttgg 
        ctgaaacta cctctagtt aagaaagat aatgagata 
        aataaagaa gataaccta agactggga ggcggagac 
        gagataaca aatttgaac gaaacagaa tgggacaaa 
        aaccagtgg caagcaatc aattttaga ctgactagc 
        atcgaaaac gcaattagt gaccaaaat gtgattatc 
        caaaaaatt caccaagaa ctcaaatcg ttaaaccaa 
        actaaacaa ggcttgagc gaagacttt aacaaggag 
        ttagattta acaatgtcc aaacaacaa ttgcaaatt 
        gcgaaaatg ttggaaaat ttgatcaat atgcaaaag 
        aataacgac cgtgaccaa caggaccat gtaatagcc 
        agtgtgtca caaatcgtt gttaaaagt gtctcagat 
        aaacttcaa aatatagta gctcatgag atgaaacat 
        gtggttgta ccaaccatt cgaaatcta gttgaaacg 
        ctaaaacat caattagat gttcaaaat agtcaaaaa 
        ttaaacagc gatatggtt ctacaagac aagatttcg 
        aaaataatc aacagcaag gcactagcg gagtcgctc 
        agtttatca gtggccaat gtgataacg cctatttta 
        gaaaaatgc taccgggat atgatcacg tcgactttg 
        gtcccgtct tgggagaaa gtctgtgga aatatgttc 
        caccaaatt aacgaaact ttcacacag ggcactaag 
        gaatatacc gcttctgtt gagcattat atggaaagg 
        caacgacgg gttcaagag agaggtaag gatctgatc 
        gttcaaatg caatcagtt tcggaaaat ttgcaaatg 
        aacacggat aagttagtc ggaactttg tcgtctgaa 
        attcacaaa cagttcaat acatccatg aagcatacg 
        caagagaat ttattacag agtatgaag gatttaatt 
        agcgaacaa attaagtta ggttttaag agtcacgct 
        tcatttctg gaagacagt gtaatcaac gcggtgcgg 
        tctcgagct gtgacccct tcaccacat gttgaatca 
        caagttact ttggcacat cttgatcat ctactggct 
        aaagaaagt tacgatgaa gcattccag ttggcgctg 
        tcggcggaa aatttgaat tatgtgatt tacgtctgt 
        gagaaggtg gatagtaat attctcttc catgggtct 
        tctccaccg cctcagaac tgtatttta gctctgata 
        caacagttg agtatggaa ttacacaaa aataccgaa 
        atcaaattg agttacata agggctgct ttggttgct 
        ctaatcccg ggatacccc cacacgaga cagtttatt 
        cccaaagtt ttgaaagat ttgctgaaa caattacaa 
        ctttttatg accacaaac ccctcctca aagtttatc 
        accgaggca aaactactt aaaatggct gtagaaagc 
        atcttggac aaatag
      mRNA sequence
      • >TC012909 RA
        ttttatttg acgtttgac gatttatct tttcttttt 
        agagtgact tttaataag ttgggataa gacacacaa 
        aatgaattc gccaccgct cgcccctca agacgacgt 
        gatccaaaa catcagtta caccgattc tgatacgga 
        atattctgc aaaaattgt gggtcatga agtaacagt 
        tcagtgtaa ctcgggaca gcataacca tggaagttc 
        taaagtgaa acttatcaa tcggattga ttataattg 
        ggagtttca ctattatcg cggacaatt ggtcgctgt 
        tcacattaa tggaaaaat tattgctta tgggatgaa 
        ggggaaaga cgggggcat gatccgagt tacgaacca 
        agagaccaa cgaaagagc actaatcaa aaacttgaa 
        aaatgatgt caaagacct gtcttttgc tttttcacg 
        ccaacagat tattttagg atgtattga cgacgaagg 
        caatgtttt catttatga aatcattga tgaacctag 
        tgcaataaa atacaaaca actactcca catattcca 
        cagtgatgt ggtcccaca ttcaagccc caactttcg 
        aattatctg gtgtcccta cttgacctg ttttgacga 
        ggaagagga gacaccaga tgatcctga aaaaatgtt 
        tgtggtatt aaattggga caaagccga aatatggaa 
        ttttggcat gattaatgc aaagtatgg gccaggtcc 
        tttacaacc aaatgaaaa ctatgaggg ttatgttga 
        aattagcca tactgctga attggtaga tgcttcatt 
        ttcgtcaga tgggaccgc tattgcaat tgcttgctt 
        agacggtta tgtgaaatt ttttcaaat ttacatggt 
        tgacaatga aaagcaaaa gtgcttgca tgagtggag 
        accacatga tggtaaacc actatcttc tatcatatt 
        tgtcgacaa tttgctgga gtgcagttc tcagtgttg 
        gaaattcac aataacggg tgcgaatca caactctga 
        gctaaagtt gtggtcttg tgaaacgtg gacttgtct 
        ccagacaat ccatttcaa accagatcc atcgaatct 
        aatccaaag tgtttatct taaaatgag tatagacta 
        ctcgggtca gtatttatt aatgtccga tctaaacaa 
        taaagtttt atacgtcct gcaattaaa acgaagcga 
        tgaagctca acaaatatc agtaacaat gatatcgca 
        atttttact accaactgc ctttttgag cttccacat 
        aaccgaagc caactgtcg caaattacg ttcgtccga 
        tatgagtga agagttttc acaagatgc cgatcaaga 
        tgacgatga tgaagacga tactgttgc agctgttgt 
        tatcaaaat gttggtgat tcagcagaa aaaattcca 
        agagtgtaa tattgtatt tcaaccaga tgcgatgtt 
        gtgtaatac tgagattat atttaatga aattaaaga 
        ggaattgga gaaagtgcc aaaattgga cgatcttca 
        gtcatccgt tactttatt aataaaaca acagcaaca 
        gcaacaatt aactttgat gactcctga cgcttttac 
        aacagctga acaaaactc tcgtcctaa tagtgtcag 
        aactagcat caatggtga tattaataa atcaaccga 
        aggcacaaa tggcgatac tgttgagaa tttgattga 
        ttttcaaag acctgaaaa ggataattt tgcaagtgg 
        aggttctag tcctagtag agaagttca ggaaattct 
        atctcttaa caattcaac gtacagcac tcaggacta 
        tttcgataa tttaacgaa aatgcaaga agaacaaga 
        aatacccca gaaggagta caaacataa cgaaaatct 
        catgtacca agagaaccc caatcctga aatggtttg 
        gctgaaact acctctagt taagaaaga taatgagat 
        aaataaaga agataacct aagactggg aggcggaga 
        cgagataac aaatttgaa cgaaacaga atgggacaa 
        aaaccagtg gcaagcaat caattttag actgactag 
        catcgaaaa cgcaattag tgaccaaaa tgtgattat 
        ccaaaaaat tcaccaaga actcaaatc gttaaacca 
        aactaaaca aggcttgag cgaagactt taacaagga 
        gttagattt aacaatgtc caaacaaca attgcaaat 
        tgcgaaaat gttggaaaa tttgatcaa tatgcaaaa 
        gaataacga ccgtgacca acaggacca tgtaatagc 
        cagtgtgtc acaaatcgt tgttaaaag tgtctcaga 
        taaacttca aaatatagt agctcatga gatgaaaca 
        tgtggttgt accaaccat tcgaaatct agttgaaac 
        gctaaaaca tcaattaga tgttcaaaa tagtcaaaa 
        attaaacag cgatatggt tctacaaga caagatttc 
        gaaaataat caacagcaa ggcactagc ggagtcgct 
        cagtttatc agtggccaa tgtgataac gcctatttt 
        agaaaaatg ctaccggga tatgatcac gtcgacttt 
        ggtcccgtc ttgggagaa agtctgtgg aaatatgtt 
        ccaccaaat taacgaaac tttcacaca gggcactaa 
        ggaatatac cgcttctgt tgagcatta tatggaaag 
        gcaacgacg ggttcaaga gagaggtaa ggatctgat 
        cgttcaaat gcaatcagt ttcggaaaa tttgcaaat 
        gaacacgga taagttagt cggaacttt gtcgtctga 
        aattcacaa acagttcaa tacatccat gaagcatac 
        gcaagagaa tttattaca gagtatgaa ggatttaat 
        tagcgaaca aattaagtt aggttttaa gagtcacgc 
        ttcatttct ggaagacag tgtaatcaa cgcggtgcg 
        gtctcgagc tgtgacccc ttcaccaca tgttgaatc 
        acaagttac tttggcaca tcttgatca tctactggc 
        taaagaaag ttacgatga agcattcca gttggcgct 
        gtcggcgga aaatttgaa ttatgtgat ttacgtctg 
        tgagaaggt ggatagtaa tattctctt ccatgggtc 
        ttctccacc gcctcagaa ctgtatttt agctctgat 
        acaacagtt gagtatgga attacacaa aaataccga 
        aatcaaatt gagttacat aagggctgc tttggttgc 
        tctaatccc gggataccc ccacacgag acagtttat 
        tcccaaagt tttgaaaga tttgctgaa acaattaca 
        actttttat gaccacaaa cccctcctc aaagtttat 
        caccgaggc aaaactact taaaatggc tgtagaaag 
        catcttgga caaatagct ggaacaatt tattttatc 
        cgcgtacgt tactgttta ttttaaccc gacttgtca 
        aaatttatt ttgaaagta ttttaactg ctgtgtcgt 
        tattactgt gaatagcaa cttgctata tttgatagg 
        ttgtgtata atttttttg aaataaact tctccaatg 
        ctgtaaaaa ttaatttta actgggcac acaaatttt 
        tgaagccga tactgttga ttagtaatg acagctggc 
        aaacttcac aaaattaaa cttactata atatgtaag 
        gttatgtcc tgtcagatg aaaattaaa acacaaatt 
        tgaaagcag ccaaaatgc aaaaaaagc cgagaaata 
        gcgtttttg ttgagaggc tccacaaag gttttgttt 
        gtacgtgca tagcggtaa caatttacg ccacttcgg 
        tactggcta tgaggggct atcgatact ttgttaaag 
        agaagcccg aattaaagc agaagcagc ttgtagagc 
        aggcaagtc ttctagcag agggatcac cggacacag 
        ctccaacca tacgagctt aaattcttg atgtagata 
        gagttttgg tactgtttt taccagaaa cggtgtagt 
        tatgtacat tattttaat ataatccat gatttaaaa 
        tatagaatg tgtgaaaaa ctgtaaaac agcttcttg 
        tttaggttt tgtgtagat agaagcaat ggataaaca 
        ataaactct ataagttac t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY