Status
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Gene information
General information
TC031678
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002274,TC002273
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 283607..290742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031678
    iBeetle screen iB_09550

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031678 RA
      MGNSSSITK PEELVRFRK LNTSFDDVD IPARPENRT 
      LTDKKGFVI FGICILLLL PFVIYALTK SDLRHLDSG 
      YDDCGNFCG YQNEKIEGI SCSGKDYTA QPFSDNLKC 
      VTQCPKDSL AGVCVDEEA QLDDIVEDS DFTETGSIV 
      WRFIVSILI TISLSIVVL VLLRFIPAI VVWSCLVLT 
      VLMLIAITF ILWFSVARA KDVNKGDFE EFSRNGVKF 
      DVLTPLTAL ASIWTIIAL VLIIIIAVV FKKIKLVIL 
      LFKEATKAL AAMPMLMLI PVTVTIIQF IVIVLFIVT 
      TIYILAARQ LVRLENGLY YYEVNGVII FAFVFNLVM 
      LNYVARFFA GINYMVISG SVSSWFFTR NKEYLGSPI 
      LTSVSNTFK YHLGTVAFG SFLMLLFGF VKMIIKPLC 
      RNACCRCIC MMCCTYVDI LFQFLSENA YIETGIHGQ 
      SYLRSAKRA TKLLLKNAK SVIAINCVG DFVLGIIVF 
      LLAIVTSVI SSTLFASSG PIYVYGPIC FIINLIIVV 
      ITFGVFQII IDTLFICFC EDSVINDGI SRPFYMTRG 
      LMEFVEHSK KLYGEKY
    CDS sequence
    • >TC031678 RA
      atgggcaac tctagcagc attacgaag ccagaagag 
      ctcgttagg ttccgaaag ttgaataca tctttcgac 
      gatgtcgac ataccagct cgtcctgaa aacaggaca 
      ctcactgat aaaaaaggt tttgtaatt tttggcata 
      tgcatcctc cttttgctt ccttttgtc atttatgca 
      ttaacaaaa tcagacctt cggcatctc gattccggt 
      tatgacgac tgcggcaac ttttgtgga taccaaaac 
      gaaaaaatt gaaggaatc tcttgctct ggaaaggat 
      tataccgca caaccattt tcagataat ttgaaatgt 
      gtgacgcaa tgccccaaa gatagttta gcaggtgtt 
      tgcgtcgac gaagaggca caacttgat gacatcgtt 
      gaggatagt gatttcact gagacaggc tcaattgta 
      tggagattt attgtttct attttgatc acaataagt 
      ttgagtatt gtagtacta gtgcttctg aggttcatt 
      cccgccatt gtagtatgg agctgttta gttcttact 
      gttttaatg ctgattgct ataacgttc atactctgg 
      tttagtgtt gctcgagcc aaagacgta aataaaggc 
      gatttcgaa gagttttct agaaatgga gtgaagttt 
      gacgtgcta acaccttta accgcatta gcatcaata 
      tggacaata attgccttg gttcttatt ataattatc 
      gcagtcgta tttaagaaa attaaattg gtaatacta 
      ctgttcaaa gaagcaact aaggctctt gctgcaatg 
      cccatgtta atgttaata ccagtaaca gtgaccatc 
      atccagttc atcgtaatt gttcttttc attgtcaca 
      actatttac atacttgct gcccgacaa cttgtgcga 
      ctagaaaac gggctttac tactacgaa gtaaatgga 
      gtaataata tttgctttt gtgttcaat ctcgtcatg 
      cttaactat gtagcacga ttttttgct ggaattaat 
      tacatggta atatctgga agtgtctcg tcttggttt 
      ttcactagg aacaaggag tatttaggt tctccaatt 
      ttgacaagt gtttccaac acttttaaa taccacctt 
      ggcactgta gcgtttggg tcttttctt atgctcttg 
      tttggtttt gttaaaatg attattaaa cctttgtgc 
      agaaatgct tgctgtaga tgcatatgt atgatgtgc 
      tgtacgtat gttgatatt ctcttccaa tttctttcg 
      gaaaatgct tatatagaa acgggcatc catggacag 
      tcttatctt agatcggca aaaagggcc actaagttg 
      ttattgaaa aatgcaaaa agtgtaatt gcgattaac 
      tgtgtgggg gattttgtt ctgggaata attgtgttt 
      ctgttagct attgtaacg tcggtcatt tccagtaca 
      ctgtttgca agttctgga cccatatac gtttatggc 
      ccgatttgt tttatcata aatttgatc atcgttgtc 
      attaccttt ggagtattt cagattata atagatact 
      ctctttatt tgcttctgt gaagactcg gttattaat 
      gatggaatc tcaagaccg ttttacatg acaagaggc 
      cttatggaa tttgttgaa cactcgaag aagttgtat 
      ggtgaaaag tactaa
    mRNA sequence
    • >TC031678 RA
      ctgttcttg ctttgactg tgcttaaac gccttacaa 
      taacaccac caaaaaata agtggttca agtatttgg 
      tgttatata ttaatatat taaatatta ataataagt 
      aatatttgg taaaatata tataaaaaa agatttttt 
      gaaacaaaa cgtgttcaa gatgtgaat gtggaaata 
      aattaaatg aaaaacggt ttattttta tttttatta 
      atgagaata atctttacc aaacgaagc tttttatat 
      atgtttggt tgttgtgct ctagtctta attaagtca 
      ggcagaact aggcccctc cttcagcgt gaatcctgt 
      agcaaaagc tctgacccc attatctaa atcattatc 
      caccactcc ctaactcat gtctggaaa cctctcatg 
      cgagcagct ttttcaact gttatttat tattccgaa 
      ttcgctcgc gatgtgacc ttcgtctat ctaaatatg 
      ccagcctca aatttcaaa gagtagtgg tactagcta 
      gcgtctgtt gcgtaactt gcagtgctt tggtctgtt 
      tttaataaa ttgattatt gtgcttttc aacacctga 
      cattgaatc aactcaata cgtatgagc ttgcataat 
      ttggtagag taaaatggg caactctag cagcattac 
      gaagccaga agagctcgt taggttccg aaagttgaa 
      tacatcttt cgacgatgt cgacatacc agctcgtcc 
      tgaaaacag gacactcac tgataaaaa aggttttgt 
      aatttttgg catatgcat cctcctttt gcttccttt 
      tgtcattta tgcattaac aaaatcaga ccttcggca 
      tctcgattc cggttatga cgactgcgg caacttttg 
      tggatacca aaacgaaaa aattgaagg aatctcttg 
      ctctggaaa ggattatac cgcacaacc attttcaga 
      taatttgaa atgtgtgac gcaatgccc caaagatag 
      tttagcagg tgtttgcgt cgacgaaga ggcacaact 
      tgatgacat cgttgagga tagtgattt cactgagac 
      aggctcaat tgtatggag atttattgt ttctatttt 
      gatcacaat aagtttgag tattgtagt actagtgct 
      tctgaggtt cattcccgc cattgtagt atggagctg 
      tttagttct tactgtttt aatgctgat tgctataac 
      gttcatact ctggtttag tgttgctcg agccaaaga 
      cgtaaataa aggcgattt cgaagagtt ttctagaaa 
      tggagtgaa gtttgacgt gctaacacc tttaaccgc 
      attagcatc aatatggac aataattgc cttggttct 
      tattataat tatcgcagt cgtatttaa gaaaattaa 
      attggtaat actactgtt caaagaagc aactaaggc 
      tcttgctgc aatgcccat gttaatgtt aataccagt 
      aacagtgac catcatcca gttcatcgt aattgttct 
      tttcattgt cacaactat ttacatact tgctgcccg 
      acaacttgt gcgactaga aaacgggct ttactacta 
      cgaagtaaa tggagtaat aatatttgc ttttgtgtt 
      caatctcgt catgcttaa ctatgtagc acgattttt 
      tgctggaat taattacat ggtaatatc tggaagtgt 
      ctcgtcttg gtttttcac taggaacaa ggagtattt 
      aggttctcc aattttgac aagtgtttc caacacttt 
      taaatacca ccttggcac tgtagcgtt tgggtcttt 
      tcttatgct cttgtttgg ttttgttaa aatgattat 
      taaaccttt gtgcagaaa tgcttgctg tagatgcat 
      atgtatgat gtgctgtac gtatgttga tattctctt 
      ccaatttct ttcggaaaa tgcttatat agaaacggg 
      catccatgg acagtctta tcttagatc ggcaaaaag 
      ggccactaa gttgttatt gaaaaatgc aaaaagtgt 
      aattgcgat taactgtgt gggggattt tgttctggg 
      aataattgt gtttctgtt agctattgt aacgtcggt 
      catttccag tacactgtt tgcaagttc tggacccat 
      atacgttta tggcccgat ttgttttat cataaattt 
      gatcatcgt tgtcattac ctttggagt atttcagat 
      tataataga tactctctt tatttgctt ctgtgaaga 
      ctcggttat taatgatgg aatctcaag accgtttta 
      catgacaag aggccttat ggaatttgt tgaacactc 
      gaagaagtt gtatggtga aaagtacta attatataa 
      agtgttaaa tgtttctac gcttcacca tatatatat 
      tatctttat aacaatttt gtatttgca aatgtgccc 
      aaacaagca gtttacata tttataaag tatattctt 
      caatatttg aaactatgt attaaagtt cttgttcat 
      attttaaga attaattta gatattaga taatcagac 
      gtttgtaca actttaatg taggtatat tgtgcttac 
      aataatttg aaagtctat atttacata aggtttagt 
      ctgaaataa atatttttt tattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY