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Gene information
General information
TC002298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 194801..200149
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002298
    iBeetle screen iB_09549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002298 RA
      MENEVNVVE SLENVGMDV EERMMKRCR LPRRQVVQS 
      KRFCAKDER NASLKKVSV ILQKSETDR TFEEAQVLN 
      ECSEVVEEV QMRWRKRDV AKRRLEEFE EPPEVLKEK 
      CLILAQAIA QAQHLVVYT GAGISTAAK IPDYRGPNG 
      IWTRLQQGK DIGAHDLSM AEPTYTHMA LSELYRNKI 
      LKYVVSQNC DGLHLRSGL PRTALSELH GNMYIEVCK 
      TCKPHKEYW RLFDVTENT ARYSHKTSR RCYVCNEPL 
      VDTIVHFGE RGSLQWPLN WAGACKNAE KATTIVCLG 
      SSLKVLKKY PWLWRMDKP AKKRPNLYI VNLQWTPKD 
      DVANVKIHG KCDQVMEAV MNLLDIKVP PYDKSKDPI 
      FAHGTLLCD AELHTTSQP LLKLEDEKK EFPVQKNGI 
      KDCSTNRDN DLSSTNSIK SEFCDTSSN SDTSPSHSE 
      STLNCLPAR ETAFSIDDI LSDRVHETS ALPRRETPS 
      PLDTSFLLS NYSLLSYNH FATNLLQCQ ILGSYSDFI 
      YYPHQTSFL YPGLHSIIN PVSDFPIPE VKNEKTSPK 
      IEPSCEFCD RNYDSTSCL FYPKIEALF SNPLFRFSK 
      TEQKEKPNV CLCCDYTTD EEDIDENKE QIKNKIQAG 
      WFGKGCRKN RRWRKK
    CDS sequence
    • >TC002298 RA
      atggaaaac gaagtaaat gttgtggag agtctcgag 
      aatgttggt atggacgtt gaagaacga atgatgaaa 
      cgatgtaga ctacctaga agacaagta gtgcaatca 
      aagaggttc tgtgccaaa gatgagaga aacgcatca 
      ctgaaaaag gtttctgtt atcctccag aaatcggag 
      actgatcga acttttgaa gaagctcag gtattaaat 
      gaatgctct gaagtagtc gaagaggtc cagatgcgg 
      tggcgtaag cgagacgta gccaaacga agattagag 
      gaatttgag gaacctcca gaagtgttg aaagaaaaa 
      tgtctaatc cttgcacaa gctatagca caggcacaa 
      catctggta gtttacact ggagctgga atcagtact 
      gcagctaaa attccagat tatagaggt cctaatggg 
      atttggaca aggcttcag caaggaaaa gacattggg 
      gctcatgac ctctcaatg gctgaacca acatacacg 
      cacatggct ttgtccgaa ttataccgc aacaaaata 
      ctcaaatat gtagtatca cagaattgt gacgggttg 
      catttgcgt tcaggtttg ccacggact gctttgtca 
      gagttgcac ggaaatatg tacattgaa gtgtgcaaa 
      acttgcaag cctcataaa gagtactgg cggttgttt 
      gatgtaacg gaaaacacc gcgcgatac tcgcacaag 
      acatcacgc aggtgctac gtgtgcaac gagcctttg 
      gtcgataca atcgttcac tttggggaa agggggagc 
      ctgcagtgg ccgctaaat tgggccggt gcttgcaaa 
      aacgcggag aaggccaca acgatcgtt tgtttgggg 
      tcgagcctc aaagttttg aaaaaatat ccttggtta 
      tggcgcatg gataagccg gcgaaaaag cggccaaat 
      ttgtacatt gtcaacttg cagtggacc ccgaaagac 
      gacgttgcc aacgtcaag atccacggg aagtgtgac 
      caggtgatg gaggccgta atgaatttg ctcgacatc 
      aaagtgccc ccatacgac aaaagcaag gaccccatt 
      tttgcccac ggaacgttg ctgtgcgat gctgagctg 
      catacgacc tctcagcca ttgctcaag ctggaagat 
      gagaaaaag gagtttcca gtacaaaaa aacggtata 
      aaagactgt tccacaaat cgtgataat gatctcagt 
      tcgacaaat tcaataaaa tcagagttc tgtgatact 
      agtagcaat agtgataca tcaccaagt cattctgaa 
      tctacgcta aattgtctt cctgcaaga gaaaccgcg 
      ttttcaatc gacgacatc cttagcgat cgtgttcac 
      gaaacttca gcattacct cgtcgagag acaccaagt 
      ccgttagat actagtttt ttattgtcg aattactcg 
      cttctcagt tacaatcac ttcgccact aatttatta 
      caatgtcag atattaggc tcatattcc gattttatt 
      tattaccct catcaaacc agtttcttg taccccggt 
      cttcatagc attattaac ccagtctca gattttcct 
      ataccggaa gtcaaaaac gaaaagacc agtccgaaa 
      atcgaaccg tcatgtgaa ttttgtgat agaaattac 
      gattcaaca agttgtttg ttttacccg aaaatcgaa 
      gctctgttt agcaatcct ctatttagg tttagtaag 
      actgaacaa aaggagaaa ccgaatgtt tgtctatgt 
      tgtgattac acaactgac gaagaagac attgatgaa 
      aacaaggag caaatcaaa aataaaatt caagcgggg 
      tggttcgga aaaggctgc cgaaaaaac agacgatgg 
      aggaaaaaa tag
    mRNA sequence
    • >TC002298 RA
      caggtgaaa atggacgtg aggggctgg cagtttgtt 
      tatgtaaaa tgttgcaac aaaaacact aataataca 
      gttaattaa aatggaaaa cgaagtaaa tgttgtgga 
      gagtctcga gaatgttgg tatggacgt tgaagaacg 
      aatgatgaa acgatgtag actacctag aagacaagt 
      agtgcaatc aaagaggtt ctgtgccaa agatgagag 
      aaacgcatc actgaaaaa ggtttctgt tatcctcca 
      gaaatcgga gactgatcg aacttttga agaagctca 
      ggtattaaa tgaatgctc tgaagtagt cgaagaggt 
      ccagatgcg gtggcgtaa gcgagacgt agccaaacg 
      aagattaga ggaatttga ggaacctcc agaagtgtt 
      gaaagaaaa atgtctaat ccttgcaca agctatagc 
      acaggcaca acatctggt agtttacac tggagctgg 
      aatcagtac tgcagctaa aattccaga ttatagagg 
      tcctaatgg gatttggac aaggcttca gcaaggaaa 
      agacattgg ggctcatga cctctcaat ggctgaacc 
      aacatacac gcacatggc tttgtccga attataccg 
      caacaaaat actcaaata tgtagtatc acagaattg 
      tgacgggtt gcatttgcg ttcaggttt gccacggac 
      tgctttgtc agagttgca cggaaatat gtacattga 
      agtgtgcaa aacttgcaa gcctcataa agagtactg 
      gcggttgtt tgatgtaac ggaaaacac cgcgcgata 
      ctcgcacaa gacatcacg caggtgcta cgtgtgcaa 
      cgagccttt ggtcgatac aatcgttca ctttgggga 
      aagggggag cctgcagtg gccgctaaa ttgggccgg 
      tgcttgcaa aaacgcgga gaaggccac aacgatcgt 
      ttgtttggg gtcgagcct caaagtttt gaaaaaata 
      tccttggtt atggcgcat ggataagcc ggcgaaaaa 
      gcggccaaa tttgtacat tgtcaactt gcagtggac 
      cccgaaaga cgacgttgc caacgtcaa gatccacgg 
      gaagtgtga ccaggtgat ggaggccgt aatgaattt 
      gctcgacat caaagtgcc cccatacga caaaagcaa 
      ggaccccat ttttgccca cggaacgtt gctgtgcga 
      tgctgagct gcatacgac ctctcagcc attgctcaa 
      gctggaaga tgagaaaaa ggagtttcc agtacaaaa 
      aaacggtat aaaagactg ttccacaaa tcgtgataa 
      tgatctcag ttcgacaaa ttcaataaa atcagagtt 
      ctgtgatac tagtagcaa tagtgatac atcaccaag 
      tcattctga atctacgct aaattgtct tcctgcaag 
      agaaaccgc gttttcaat cgacgacat ccttagcga 
      tcgtgttca cgaaacttc agcattacc tcgtcgaga 
      gacaccaag tccgttaga tactagttt tttattgtc 
      gaattactc gcttctcag ttacaatca cttcgccac 
      taatttatt acaatgtca gatattagg ctcatattc 
      cgattttat ttattaccc tcatcaaac cagtttctt 
      gtaccccgg tcttcatag cattattaa cccagtctc 
      agattttcc tataccgga agtcaaaaa cgaaaagac 
      cagtccgaa aatcgaacc gtcatgtga attttgtga 
      tagaaatta cgattcaac aagttgttt gttttaccc 
      gaaaatcga agctctgtt tagcaatcc tctatttag 
      gtttagtaa gactgaaca aaaggagaa accgaatgt 
      ttgtctatg ttgtgatta cacaactga cgaagaaga 
      cattgatga aaacaagga gcaaatcaa aaataaaat 
      tcaagcggg gtggttcgg aaaaggctg ccgaaaaaa 
      cagacgatg gaggaaaaa atagtttta attcgtcag 
      tattaatgt gaattttgt attattttt ttacttttt 
      atttattta tttactgag tgtttattt ataaataaa 
      aacttgggt ataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY