Status
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Gene information
General information
TC011554 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451203.1: 1170..5657
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011554
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_09546

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011554 RA
        MGTRRSSVS SKRSRLDDD SNEEFDEVE HVKAIKDDL 
        LKILFNEAN KCNKGAIEQ ILNRFEDLE LVTMRLIRD 
        NSFLEGRIK EMKKYSTTG EEIRNQKEG GLVVPVSNN 
        VVKSYAIVV KGKDENASK IKEKLMKVE TDAKIQAVK 
        QSRAGGLVI QTTNEKDLN KIKDQINKQ EQGWTIEEA 
        RRRDPTLLV YDVPEKIDN EEFAKEVYK KNVAALMGE 
        EEFIKEFRV EPYEWKQNV RGMPLHFRV ISSKKAAIV 
        VNDESIEVM EVGEFRREW AVCAWLKGD FGECVVCSL 
        YCVYGEGME DKAAYLEEL WRKFGRLKM LVGLDANAV 
        SPLWHSKWE GWREDERVV RGRVLEEVI LREGIAVLN 
        SPSDEYTYS GPGGQSDID VTLCTDGFL GLVRKWCVR 
        DEVSCSDHN LIELELGYH IDRDCGPPP GGWVVCEGD 
        WPKFDACFL DEVFCVERE FALLDAEGM VELMTKMVR 
        RAGDRSFKV NRGHSNRRV RWWNERLGD LRARCRRSR 
        RVYQRLRRH NAEGQDAAR ARYIEEWNE YKNEIRKAK 
        EEDWRRFVD RVGNVDPWG GVYKIIRGR KGRICMGAV 
        RKDNGEMTV GWEDTASAL LNEFFPPDE KVRDEVRLV 
        GGARMEPVT EEEVEVAVK RINGKKAPG LDGIKGDIV 
        KRVWALRPD LMHRMYEQC VLEGTFPRE WKKANVVVL 
        LKSPDKDRA EIRSYRPIC LLSVFGKVM ERIMVNRLL 
        GGLEGSMSD CQYGFTVGR GTEDAWLKV MDVVEGSAC 
        KYVLGMFID FKGAFDFLR WCRILDVLG AVGCREIEL 
        WKSYFCERK VCYRNARGE MVWKDVSRG CPQGSICGP 
        FVWNLCMNE LLRGLGELG AQVVAYADD LLVLVEGNS 
        RRDLESRGS EYLGAVLNW GRHYGVEVS ATKSSTLIL 
        KGTLSSTRP VLVKAQGIS LKSDKKVKY LGIHVEERL 
        KFGNHLKLL RKKIVDVAS GLRRVIRDD WGLGRTAIK 
        TIFNGLLMA CALYGSSVW WKTLRTGKG VRLLNTCER 
        AGLLVCLRV CRTVSTDAM EVLMGVLPW RHQALLRTV 
        MFLRSRGRP VGLVGVVED RELEDLGRS GVRDLLRDR 
        LFARWQREW DGSETGRVL YCFVPDVET VRRCKWFWV 
        GMHEGYIMT GQGSMNAWL HDRGLSESP ECECGAPRE 
        DWMHVLVEC VLYEDLRDL EAMGLVVEN DGEINGLRE 
        IVMNEESFK TYVEFARNV FARRVRMVP LLTPVRLSG 
        SSDPAYLSW SSSAGLFSQ SNCSLMAAG SPVSCVELK 
        NWYRGGR
      CDS sequence
      • >TC011554 RA
        atggggact agaagaagt agcgtgtcc agtaaaagg 
        tcaaggctc gacgatgac agtaatgag gaatttgac 
        gaggtggag catgtcaaa gcgattaag gacgacctt 
        ctgaagatc ctttttaat gaggcaaac aagtgtaac 
        aagggcgca attgaacag attctaaat aggttcgag 
        gacctggaa ctggtgaca atgcgtctg attagagat 
        aacagtttc ttagagggg aggattaaa gaaatgaag 
        aagtattcc actacagga gaggaaata aggaatcaa 
        aaggaggga ggactggtg gtgcccgta agtaacaac 
        gtggtgaaa agttatgct atagtagtg aaaggaaag 
        gatgagaac gcctcaaaa ataaaggaa aaattgatg 
        aaggttgaa acggacgcc aaaattcag gcggtaaag 
        caatcaaga gcaggaggg ttggttatt cagaccacc 
        aatgaaaaa gacttgaat aagataaag gaccagatt 
        aataaacag gagcagggc tggacgatt gaagaagcg 
        aggagaaga gacccaaca ttgttggtc tacgatgtc 
        ccagagaaa atcgataat gaagaattc gctaaagaa 
        gtgtacaaa aagaacgtg gccgcgttg atgggagag 
        gaggaattt ataaaggag tttagagtg gaaccgtat 
        gaatggaag cagaatgta agggggatg ccacttcat 
        ttcagagtg atatccagt aagaaagcg gcgatcgta 
        gtgaatgat gaaagtata gaagtgatg gaggttgga 
        gaatttaga agggagtgg gctgtatgt gcttggttg 
        aaaggtgac tttggtgaa tgtgtggta tgttcgctt 
        tattgtgtg tatggtgag ggaatggag gacaaggct 
        gcgtaccta gaggagctt tggcgtaaa ttcggtaga 
        ctaaagatg ctggtaggg cttgatgcc aatgcggtg 
        tcccccctg tggcacagc aaatgggaa ggatggagg 
        gaggatgaa agggttgtt cgtggaagg gtactggag 
        gaggttatt ttgagggag gggattgct gtcctaaat 
        tctcccagt gatgaatac acttactcc ggtccaggg 
        ggacagagt gatattgac gtcacgctg tgtaccgat 
        gggtttttg ggactggta cgcaaatgg tgtgtgaga 
        gatgaggtt agttgtagc gaccataac ttaatcgaa 
        ttggagttg ggataccat attgatcgg gattgtggg 
        cctcctcct ggaggatgg gtggtatgc gagggcgac 
        tggccgaaa tttgacgca tgttttctc gatgaggtg 
        ttttgcgta gagagagag tttgccctt cttgatgct 
        gagggtatg gttgagcta atgactaaa atggtgcgg 
        cgcgcaggt gacaggtcc tttaaagtg aatcgggga 
        cactcaaat agacgggta cggtggtgg aacgagagg 
        ctgggggat cttcgggct agatgcaga cgttcgagg 
        agggtttat cagcgcctt cggaggcat aatgcagag 
        gggcaggac gcggccagg gcacggtac attgaggaa 
        tggaatgaa tacaaaaac gagataagg aaggccaaa 
        gaggaggac tggagaagg tttgtggac agggtagga 
        aatgtggac ccatggggt ggggtttat aaaattatt 
        aggggtagg aagggtaga atctgtatg ggagctgtt 
        aggaaagat aatggggaa atgacggtt gggtgggag 
        gatacggca agtgctttg ttgaatgaa ttctttccc 
        ccagacgaa aaggtaagg gatgaggtg agactggtg 
        gggggggcg agaatggaa ccggtaacc gaggaagag 
        gttgaggtt gcggtcaaa agaattaat ggtaagaag 
        gctccggga ttagatgga ataaaaggt gacattgtt 
        aagcgtgtc tgggcttta aggccggat ctgatgcat 
        cgtatgtat gagcagtgt gtgttggag ggaaccttc 
        cccagggaa tggaagaaa gcgaatgtt gtggttctg 
        ttgaagtct ccggacaaa gacagggct gagataaga 
        tcatataga cctatatgc ttgttgagt gtgtttggt 
        aaagtcatg gaaaggatt atggtgaat aggcttctt 
        ggagggttg gaagggtct atgagtgat tgccaatat 
        ggtttcacg gttggtaga ggtacagag gatgcatgg 
        ttgaaagtg atggatgtt gtggaggga tctgcctgc 
        aagtatgtg ttgggaatg tttatcgat tttaaggga 
        gcgtttgat ttcttgaga tggtgcaga attttggat 
        gtgctgggt gctgttggc tgtcgagaa atagaatta 
        tggaagagc tacttctgt gagagaaag gtttgttac 
        aggaatgca cgtggtgaa atggtatgg aaagacgta 
        agtagagga tgtcctcag ggttcgata tgtggtccg 
        tttgtatgg aatttgtgt atgaatgag cttttgagg 
        ggccttggg gaattggga gcccaagtg gtggcttat 
        gccgatgac cttctcgtc ctggtagag ggtaacagt 
        aggagggac ttggagagc agaggtagc gaataccta 
        ggtgctgtt ctcaattgg ggcagacat tatggcgtg 
        gaggtctct gccacgaaa tcatctacg ttaatctta 
        aaggggaca ctgtcctct accagacct gttctggtt 
        aaggcgcag ggtatatca cttaaaagt gacaaaaaa 
        gttaagtac ctgggtatc cacgttgag gaacgatta 
        aaatttggg aaccaccta aaattgctc cggaaaaaa 
        attgtagac gttgcctcg ggactgaga agggtcatc 
        agggatgat tggggactt ggcagaaca gccattaag 
        acaattttt aacggcctt ctgatggca tgtgcgttg 
        tatggttca agtgtgtgg tggaagacc ctgaggacg 
        ggaaagggt gtgagatta ttgaatact tgcgagagg 
        gctggactg cttgtgtgt ttgagagtg tgcaggacg 
        gtctccacg gacgcgatg gaggtacta atgggagtg 
        ttgccatgg cgccatcag gcgttgttg cgcactgta 
        atgtttctt agaagtcgg ggaagacct gtggggctg 
        gttggggtg gtagaggat cgcgaacta gaggacctg 
        gggaggtct ggggttcgc gatttactg agggatagg 
        ttatttgcc agatggcaa cgggaatgg gatggctcc 
        gaaacgggc agggttctt tattgcttc gttcctgat 
        gtggagact gtccgcagg tgtaaatgg ttttgggtg 
        ggtatgcat gagggatat ataatgaca gggcagggc 
        agcatgaat gcatggctg catgataga ggattgtcg 
        gagagtcca gaatgtgaa tgtggggct ccccgcgag 
        gattggatg catgttttg gttgaatgt gtactgtat 
        gaagatttg agggatttg gaagcaatg ggattagta 
        gtggagaac gatggagaa ataaatggt ttgagagaa 
        attgttatg aatgaggag agttttaaa acctatgtt 
        gagtttgct aggaacgtg tttgcaagg cgtgtgaga 
        atggtgcct cttctgaca ccagtgaga ctttcgggg 
        agtagcgac cccgcctac ctgagctgg tctagcagt 
        gccggattg ttcagtcaa tccaactgt agcctcatg 
        gccgcaggt tcaccagtt tcctgtgtc gaactgaaa 
        aactggtac cgcgggggt cggtga
      mRNA sequence
      • >TC011554 RA
        taattttgt tgaacgggg tcaaaattt ttttggaat 
        cataatggg gactagaag aagtagcgt gtccagtaa 
        aaggtcaag gctcgacga tgacagtaa tgaggaatt 
        tgacgaggt ggagcatgt caaagcgat taaggacga 
        ccttctgaa gatcctttt taatgaggc aaacaagtg 
        taacaaggg cgcaattga acagattct aaataggtt 
        cgaggacct ggaactggt gacaatgcg tctgattag 
        agataacag tttcttaga ggggaggat taaagaaat 
        gaagaagta ttccactac aggagagga aataaggaa 
        tcaaaagga gggaggact ggtggtgcc cgtaagtaa 
        caacgtggt gaaaagtta tgctatagt agtgaaagg 
        aaaggatga gaacgcctc aaaaataaa ggaaaaatt 
        gatgaaggt tgaaacgga cgccaaaat tcaggcggt 
        aaagcaatc aagagcagg agggttggt tattcagac 
        caccaatga aaaagactt gaataagat aaaggacca 
        gattaataa acaggagca gggctggac gattgaaga 
        agcgaggag aagagaccc aacattgtt ggtctacga 
        tgtcccaga gaaaatcga taatgaaga attcgctaa 
        agaagtgta caaaaagaa cgtggccgc gttgatggg 
        agaggagga atttataaa ggagtttag agtggaacc 
        gtatgaatg gaagcagaa tgtaagggg gatgccact 
        tcatttcag agtgatatc cagtaagaa agcggcgat 
        cgtagtgaa tgatgaaag tatagaagt gatggaggt 
        tggagaatt tagaaggga gtgggctgt atgtgcttg 
        gttgaaagg tgactttgg tgaatgtgt ggtatgttc 
        gctttattg tgtgtatgg tgagggaat ggaggacaa 
        ggctgcgta cctagagga gctttggcg taaattcgg 
        tagactaaa gatgctggt agggcttga tgccaatgc 
        ggtgtcccc cctgtggca cagcaaatg ggaaggatg 
        gagggagga tgaaagggt tgttcgtgg aagggtact 
        ggaggaggt tattttgag ggaggggat tgctgtcct 
        aaattctcc cagtgatga atacactta ctccggtcc 
        agggggaca gagtgatat tgacgtcac gctgtgtac 
        cgatgggtt tttgggact ggtacgcaa atggtgtgt 
        gagagatga ggttagttg tagcgacca taacttaat 
        cgaattgga gttgggata ccatattga tcgggattg 
        tgggcctcc tcctggagg atgggtggt atgcgaggg 
        cgactggcc gaaatttga cgcatgttt tctcgatga 
        ggtgttttg cgtagagag agagtttgc ccttcttga 
        tgctgaggg tatggttga gctaatgac taaaatggt 
        gcggcgcgc aggtgacag gtcctttaa agtgaatcg 
        gggacactc aaatagacg ggtacggtg gtggaacga 
        gaggctggg ggatcttcg ggctagatg cagacgttc 
        gaggagggt ttatcagcg ccttcggag gcataatgc 
        agaggggca ggacgcggc cagggcacg gtacattga 
        ggaatggaa tgaatacaa aaacgagat aaggaaggc 
        caaagagga ggactggag aaggtttgt ggacagggt 
        aggaaatgt ggacccatg gggtggggt ttataaaat 
        tattagggg taggaaggg tagaatctg tatgggagc 
        tgttaggaa agataatgg ggaaatgac ggttgggtg 
        ggaggatac ggcaagtgc tttgttgaa tgaattctt 
        tcccccaga cgaaaaggt aagggatga ggtgagact 
        ggtgggggg ggcgagaat ggaaccggt aaccgagga 
        agaggttga ggttgcggt caaaagaat taatggtaa 
        gaaggctcc gggattaga tggaataaa aggtgacat 
        tgttaagcg tgtctgggc tttaaggcc ggatctgat 
        gcatcgtat gtatgagca gtgtgtgtt ggagggaac 
        cttccccag ggaatggaa gaaagcgaa tgttgtggt 
        tctgttgaa gtctccgga caaagacag ggctgagat 
        aagatcata tagacctat atgcttgtt gagtgtgtt 
        tggtaaagt catggaaag gattatggt gaataggct 
        tcttggagg gttggaagg gtctatgag tgattgcca 
        atatggttt cacggttgg tagaggtac agaggatgc 
        atggttgaa agtgatgga tgttgtgga gggatctgc 
        ctgcaagta tgtgttggg aatgtttat cgattttaa 
        gggagcgtt tgatttctt gagatggtg cagaatttt 
        ggatgtgct gggtgctgt tggctgtcg agaaataga 
        attatggaa gagctactt ctgtgagag aaaggtttg 
        ttacaggaa tgcacgtgg tgaaatggt atggaaaga 
        cgtaagtag aggatgtcc tcagggttc gatatgtgg 
        tccgtttgt atggaattt gtgtatgaa tgagctttt 
        gaggggcct tggggaatt gggagccca agtggtggc 
        ttatgccga tgaccttct cgtcctggt agagggtaa 
        cagtaggag ggacttgga gagcagagg tagcgaata 
        cctaggtgc tgttctcaa ttggggcag acattatgg 
        cgtggaggt ctctgccac gaaatcatc tacgttaat 
        cttaaaggg gacactgtc ctctaccag acctgttct 
        ggttaaggc gcagggtat atcacttaa aagtgacaa 
        aaaagttaa gtacctggg tatccacgt tgaggaacg 
        attaaaatt tgggaacca cctaaaatt gctccggaa 
        aaaaattgt agacgttgc ctcgggact gagaagggt 
        catcaggga tgattgggg acttggcag aacagccat 
        taagacaat ttttaacgg ccttctgat ggcatgtgc 
        gttgtatgg ttcaagtgt gtggtggaa gaccctgag 
        gacgggaaa gggtgtgag attattgaa tacttgcga 
        gagggctgg actgcttgt gtgtttgag agtgtgcag 
        gacggtctc cacggacgc gatggaggt actaatggg 
        agtgttgcc atggcgcca tcaggcgtt gttgcgcac 
        tgtaatgtt tcttagaag tcggggaag acctgtggg 
        gctggttgg ggtggtaga ggatcgcga actagagga 
        cctggggag gtctggggt tcgcgattt actgaggga 
        taggttatt tgccagatg gcaacggga atgggatgg 
        ctccgaaac gggcagggt tctttattg cttcgttcc 
        tgatgtgga gactgtccg caggtgtaa atggttttg 
        ggtgggtat gcatgaggg atatataat gacagggca 
        gggcagcat gaatgcatg gctgcatga tagaggatt 
        gtcggagag tccagaatg tgaatgtgg ggctccccg 
        cgaggattg gatgcatgt tttggttga atgtgtact 
        gtatgaaga tttgaggga tttggaagc aatgggatt 
        agtagtgga gaacgatgg agaaataaa tggtttgag 
        agaaattgt tatgaatga ggagagttt taaaaccta 
        tgttgagtt tgctaggaa cgtgtttgc aaggcgtgt 
        gagaatggt gcctcttct gacaccagt gagactttc 
        ggggagtag cgaccccgc ctacctgag ctggtctag 
        cagtgccgg attgttcag tcaatccaa ctgtagcct 
        catggccgc aggttcacc agtttcctg tgtcgaact 
        gaaaaactg gtaccgcgg gggtcggtg agccccgga 
        gccgagggc tccggccga ccattggtg ttggactcg 
        cgggggcag tggttgaaa gct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY