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Gene information
General information
TC002073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6127588..6128650
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002073
    iBeetle screen iB_09544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    egg chamber mostlynot present - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1

    egg chamber potentiallydegenerated - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002073 RA
      MDESPEALK ELGNSAVKN QKYEEAILY 
      YTQALKSDS NNYTLYSNR SFAFLKVQQ 
      YYFAMQDAN ETIRLNPNW PKGYFRRAE 
      VEYSAKHFV EACESYQKA LSLKPDDIN 
      IIEALSRAK RQIAKERKA DEVIPWLGA 
      GIGIVIGVI IVIADCILT HKPTHPLLM 
      AFVTIIIAM VGYGIAKTY RYYVRCQRD 
      GLLEPPPDL MSEDDRDET SEKETKHTP 
      RYTKSQARQ RYKKGKL
    CDS sequence
    • >TC002073 RA
      atggacgag tcaccggaa gcgttaaaa gaactggga 
      aacagcgct gtcaaaaat caaaaatat gaagaagcg 
      attttatac tacactcaa gcccttaag agcgattct 
      aacaactac accctgtat agtaatcga tcttttgca 
      ttcctcaag gtccaacaa tattatttt gcaatgcaa 
      gatgcaaac gaaacgatc agactgaac ccaaattgg 
      cccaaaggt tatttccgc agggctgag gtcgaatat 
      tcggccaaa cactttgtg gaagcctgt gagtcgtac 
      caaaaagcc ttaagttta aaacccgac gatatcaat 
      attattgag gcccttagt agggccaaa agacagatt 
      gccaaggag aggaaggcg gacgaggtc ataccatgg 
      ctgggggcc ggcattggc atcgtcata ggggtgata 
      atcgtcata gctgactgt attctcacc cacaaaccg 
      acacaccct cttttgatg gctttcgtc accatcatt 
      atagccatg gtggggtac gggatcgcc aaaacctac 
      agatattac gtgaggtgt caaagggac gggctacta 
      gagccccca cctgaccta atgagtgaa gatgatagg 
      gacgaaacg agcgagaaa gagacgaaa cataccccc 
      agatacacc aaatcgcaa gcgagacag agatacaag 
      aagggaaag ttgtga
    mRNA sequence
    • >TC002073 RA
      ttaccgtca cttgtcaca atgacagtg gcgaggtta 
      tgttcatga gcaatcaaa accgagcac tttaataaa 
      taatggacg agtcaccgg aagcgttaa aagaactgg 
      gaaacagcg ctgtcaaaa atcaaaaat atgaagaag 
      cgattttat actacactc aagccctta agagcgatt 
      ctaacaact acaccctgt atagtaatc gatcttttg 
      cattcctca aggtccaac aatattatt ttgcaatgc 
      aagatgcaa acgaaacga tcagactga acccaaatt 
      ggcccaaag gttatttcc gcagggctg aggtcgaat 
      attcggcca aacactttg tggaagcct gtgagtcgt 
      accaaaaag ccttaagtt taaaacccg acgatatca 
      atattattg aggccctta gtagggcca aaagacaga 
      ttgccaagg agaggaagg cggacgagg tcataccat 
      ggctggggg ccggcattg gcatcgtca taggggtga 
      taatcgtca tagctgact gtattctca cccacaaac 
      cgacacacc ctcttttga tggctttcg tcaccatca 
      ttatagcca tggtggggt acgggatcg ccaaaacct 
      acagatatt acgtgaggt gtcaaaggg acgggctac 
      tagagcccc cacctgacc taatgagtg aagatgata 
      gggacgaaa cgagcgaga aagagacga aacataccc 
      ccagataca ccaaatcgc aagcgagac agagataca 
      agaagggaa agttgtgaa atatcggcc tcacattcc 
      gttaacccc atttatggt tgttgctta tgtaagatg 
      gtacataat gtgtttgat gttgttgaa taaaattat 
      ttaaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY