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Gene information
General information
TC011604 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15236114..15237491
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011604
    iBeetle screen iB_09542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3

    ovary without detectable defects - Number of animals showing the phenotype: 1

    follicular epithelium potentially not present - Number of animals showing the phenotype: 1

    egg chamber potentiallyfused - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011604 RA
      MPPSQKIQV KLTDPIDPL IETEQVFIP FPLSQEVKK 
      SSQWAAWAR NTPFGILSV SVLEIIFYA ASDEKTQKL 
      LRFEPHKLT EIWRFVTYM LLHEDWIHL TLNIFMQVL 
      FAYFLEARH GSVRILVLY VTGGITGVL GAACFHPDL 
      VIGASGGVY ALLISHISD IFLNFETLN YKIYRSACI 
      GIIVIFDII YNVLHANFK KEPLISWGA HMIGGLAGL 
      FLGLVLFKQ KPSIARTRR LVLFLVGLL LYVILLISL 
      VIITVQIKK CTPKDAIRK RYVYFC
    CDS sequence
    • >TC011604 RA
      atgccaccg tcgcaaaaa atccaagtg aaattaacc 
      gaccccatc gacccctta atcgaaacc gagcaagtt 
      tttatcccc tttccctta tcacaagaa gtgaagaaa 
      tcgtcgcaa tgggccgct tgggcaaga aacacacct 
      ttcggtatt ttgtcagtc agtgtttta gagataatc 
      ttctacgct gcctcggat gaaaaaacc caaaaactg 
      ctccgcttc gaaccgcac aaactgacc gaaatttgg 
      cgattcgtc acctacatg cttttgcac gaagactgg 
      atccacctg actctcaat attttcatg caagtgctt 
      ttcgcctat tttttagaa gcgcgacac ggaagcgtg 
      cggattctt gttttgtac gtgaccggg ggaatcacc 
      ggagtcttg ggagcggcc tgttttcac cccgacttg 
      gtcattggg gcctccggg ggcgtgtat gcgctttta 
      atttcgcac atttccgat atttttcta aactttgaa 
      accttgaat tacaaaatc tacagatct gcgtgtatc 
      ggaataatc gtcattttc gacattatc tacaacgtg 
      ttacatgcc aattttaaa aaggagcct ttgatatcg 
      tggggggcc cacatgatc gggggcctc gcaggcctg 
      tttttgggt ctagtgctc ttcaagcaa aaaccgtca 
      attgcacgc acacggaga ttagtttta tttttggtg 
      ggtttatta ctttacgta attttattg atctcgctt 
      gtaataata accgttcag attaaaaaa tgtactccg 
      aaggatgcc atacgcaag cggtatgtt tatttttgc 
      tga
    mRNA sequence
    • >TC011604 RA
      taccttctt aaacgcaag ttttttgtt tttataact 
      ttaactatt tatagttgg ttcagtttt attcaaact 
      gtatgcgga gtagatgcc gcataataa ttgctcaaa 
      tagtacgac acaaaatcg ggcctattt ccctccact 
      ttcggacat gttccattt tcgacccaa atttggtaa 
      attttaatt atgccaccg tcgcaaaaa atccaagtg 
      aaattaacc gaccccatc gacccctta atcgaaacc 
      gagcaagtt tttatcccc tttccctta tcacaagaa 
      gtgaagaaa tcgtcgcaa tgggccgct tgggcaaga 
      aacacacct ttcggtatt ttgtcagtc agtgtttta 
      gagataatc ttctacgct gcctcggat gaaaaaacc 
      caaaaactg ctccgcttc gaaccgcac aaactgacc 
      gaaatttgg cgattcgtc acctacatg cttttgcac 
      gaagactgg atccacctg actctcaat attttcatg 
      caagtgctt ttcgcctat tttttagaa gcgcgacac 
      ggaagcgtg cggattctt gttttgtac gtgaccggg 
      ggaatcacc ggagtcttg ggagcggcc tgttttcac 
      cccgacttg gtcattggg gcctccggg ggcgtgtat 
      gcgctttta atttcgcac atttccgat atttttcta 
      aactttgaa accttgaat tacaaaatc tacagatct 
      gcgtgtatc ggaataatc gtcattttc gacattatc 
      tacaacgtg ttacatgcc aattttaaa aaggagcct 
      ttgatatcg tggggggcc cacatgatc gggggcctc 
      gcaggcctg tttttgggt ctagtgctc ttcaagcaa 
      aaaccgtca attgcacgc acacggaga ttagtttta 
      tttttggtg ggtttatta ctttacgta attttattg 
      atctcgctt gtaataata accgttcag attaaaaaa 
      tgtactccg aaggatgcc atacgcaag cggtatgtt 
      tatttttgc tgatatttt ttttataac gagtgtttg 
      gctataaat gtgcggtag caaagtaaa gcgtgaaat 
      tacacaact tatagatgt ttgcggcta tttttagac 
      gttggtttt gtaaaaaag tgaaaaata aacgattaa 
      acaaactgc tgcttactt ggttgcacg ctcatcaca 
      acaccacta gctcaactg agatttttt taacgtttt 
      taacactta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY