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Gene information
General information
TC011607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15270974..15272531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011607
    iBeetle screen iB_09540

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Constriction often separates head and thorax from abdomen segments.
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011607 RA
      MTRRVVVTG MGVLAPVGL NLTQAWATI TSGKSGISQ 
      LEGPAYAPL PCRIAGLIK EKGARIDLT SRFKKTELK 
      SMGAATAYA LLAAHEALT DSGLVSPPD SVKLRTGVA 
      VGMGMVDLE DICATSDAL KRGYNQVSP FFVPRILPN 
      MCAGQISIK YGFRGPNHA VSTACATGA HAIGDSFRF 
      IRHGDADVM VCGGAEACI SPLAIAAFC RLRALSTGF 
      NDSPQKGSR PFDKARDGF VMGEGSAIL ILEELQHAK 
      DRGAKIYAE ILGYGLSGD ASHLTAPRS DGTGAILAM 
      SRAVEDAGV DKGAVGYIN AHATSTPIG DAIETTAIK 
      TLFKDYLGP NFAVSSTKG AHGHLLGAA GNLETVFTV 
      KAVEEGVLP PTINLENCE DSSLNFVPN QSQPWAPTS 
      PRVALKNAF GFGGTNACL CISQFVN
    CDS sequence
    • >TC011607 RA
      atgacgcgc cgagtcgtc gtgacgggc atgggggtc 
      ctggccccc gtcggcctt aacctcact caggcctgg 
      gcgaccata acttcgggc aaatcgggc atttcccag 
      ctcgagggc cccgcgtac gcccccttg ccgtgccga 
      atcgccggc ttgatcaaa gaaaagggg gctcgaatc 
      gacctaacc tcccgtttc aaaaaaacc gaactcaaa 
      tcaatgggg gctgcgacc gcttacgcc ctccttgcc 
      gcccacgag gccttgacc gattccggc cttgtgagc 
      ccccctgac agtgttaag ctccggaca ggggtcgcc 
      gtcggtatg gggatggtc gatttggag gatatttgc 
      gcaactagt gacgccctc aagcggggc tacaaccaa 
      gtgagcccc tttttcgtc ccgcggatt ctccccaac 
      atgtgcgcc gggcagatt agcatcaag tatggtttc 
      agggggccc aaccatgcc gtttcaacg gcgtgtgcc 
      acgggggct cacgctatt ggagactcg ttcaggttt 
      ataagacac ggggatgcg gatgtgatg gtgtgtggg 
      ggcgccgag gcgtgcatt tcgcccctt gctatcgcg 
      gcgttttgc cggctgagg gcactaagt accgggttt 
      aatgacagc ccccagaag gggtcccgg ccgtttgat 
      aaagcgcgc gatgggttt gtgatgggt gaaggtagt 
      gcgatttta attttggaa gagttgcaa catgccaaa 
      gacaggggg gctaaaatt tatgcggag attttgggg 
      tatgggtta tcgggggac gcttcgcat ttaacggcg 
      cctaggtcc gacgggacg ggggctatt ttagccatg 
      agccgggcg gtggaagac gccggtgtg gataagggg 
      gctgtgggg tatatcaat gcccatgca acttcaact 
      ccaattggg gatgccatt gagacaact gcgattaaa 
      accttgttt aaggattat ttggggccc aatttcgcc 
      gtgtcgtcg actaagggg gctcatggc catttgtta 
      ggggcggcg gggaacctc gaaactgtc tttacggtt 
      aaagcggtt gaggaaggg gtgctaccg cctacaatt 
      aatctcgaa aattgtgaa gattcaagt ttgaacttt 
      gtgccaaat cagagccaa ccttgggcc cccacaagt 
      ccaagggtg gcccttaaa aatgcgttt ggcttcggg 
      ggcaccaac gcttgtctc tgtatctcc caatttgta 
      aattaa
    mRNA sequence
    • >TC011607 RA
      acgtgcaaa tggacgaaa ttatttttt ttaacttat 
      tactttgta gttgtgtta ataatcaat tgatcagtt 
      acttagttt tgaatcaat tacactcga atatcaatt 
      gaaatttac caataggcc tcaaataaa tactttacc 
      caaataact aaaccacaa aacgtctaa ctttaagtg 
      aggttttgt tgcaaaatt tattaataa aaaaccgat 
      aataccatg tcttaatta atagacacg acgattgga 
      ccagttatt tgtacaatg acgcgccga gtcgtcgtg 
      acgggcatg ggggtcctg gcccccgtc ggccttaac 
      ctcactcag gcctgggcg accataact tcgggcaaa 
      tcgggcatt tcccagctc gagggcccc gcgtacgcc 
      cccttgccg tgccgaatc gccggcttg atcaaagaa 
      aagggggct cgaatcgac ctaacctcc cgtttcaaa 
      aaaaccgaa ctcaaatca atgggggct gcgaccgct 
      tacgccctc cttgccgcc cacgaggcc ttgaccgat 
      tccggcctt gtgagcccc cctgacagt gttaagctc 
      cggacaggg gtcgccgtc ggtatgggg atggtcgat 
      ttggaggat atttgcgca actagtgac gccctcaag 
      cggggctac aaccaagtg agccccttt ttcgtcccg 
      cggattctc cccaacatg tgcgccggg cagattagc 
      atcaagtat ggtttcagg gggcccaac catgccgtt 
      tcaacggcg tgtgccacg ggggctcac gctattgga 
      gactcgttc aggtttata agacacggg gatgcggat 
      gtgatggtg tgtgggggc gccgaggcg tgcatttcg 
      ccccttgct atcgcggcg ttttgccgg ctgagggca 
      ctaagtacc gggtttaat gacagcccc cagaagggg 
      tcccggccg tttgataaa gcgcgcgat gggtttgtg 
      atgggtgaa ggtagtgcg attttaatt ttggaagag 
      ttgcaacat gccaaagac aggggggct aaaatttat 
      gcggagatt ttggggtat gggttatcg ggggacgct 
      tcgcattta acggcgcct aggtccgac gggacgggg 
      gctatttta gccatgagc cgggcggtg gaagacgcc 
      ggtgtggat aagggggct gtggggtat atcaatgcc 
      catgcaact tcaactcca attggggat gccattgag 
      acaactgcg attaaaacc ttgtttaag gattatttg 
      gggcccaat ttcgccgtg tcgtcgact aagggggct 
      catggccat ttgttaggg gcggcgggg aacctcgaa 
      actgtcttt acggttaaa gcggttgag gaaggggtg 
      ctaccgcct acaattaat ctcgaaaat tgtgaagat 
      tcaagtttg aactttgtg ccaaatcag agccaacct 
      tgggccccc acaagtcca agggtggcc cttaaaaat 
      gcgtttggc ttcgggggc accaacgct tgtctctgt 
      atctcccaa tttgtaaat taaacttgg attttttta 
      aacttcttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY