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Gene information
General information
TC012868 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15388157..15390347
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012868
    iBeetle screen iB_09539

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012868 RA
      MVGSRVYVG GLPYGTTER DLERFFRGY GRMRDVLIK 
      NGYGFVEFD DHRDADDAV YELNGKKLL GERVTVERA 
      RGTPRGRDQ WSSRSDHRS HERYGPPTR TNYRLIVEN 
      LSSRISWQD LKDYMRQAG EVTYADAHK QHRNEGVVE 
      FASYSDLKN AIEKLDDTE LNGRRIRLI EDRKRSRRS 
      GSYSSRSRS RSRSRSRSR SRSKSRSRS ASAKPNSKS 
      RSRSNSRKK SVDRSRSNS RKKSVERSR SRSRSPKRS 
      REPSKEASR SRSRSPSKE RSKSRSRSA SPN
    CDS sequence
    • >TC012868 RA
      atggtaggg tctcgcgtg tacgtcggg gggttgccg 
      tacgggacc accgagcgg gacttagag cgctttttc 
      aggggctac gggcgcatg cgggacgtg ctcatcaaa 
      aacggctac gggtttgtt gaatttgat gatcatcgc 
      gatgccgac gatgctgta tatgaatta aacggtaaa 
      aaactttta ggggagcgt gtgactgtc gaacgggcc 
      cgagggaca ccccgaggt cgagaccaa tggtccagc 
      cgcagcgac caccgctcc catgaacgc tacgggccc 
      cccaccaga accaattac cgacttatc gtcgaaaac 
      ctctcgtct cgcattagc tggcaagac ctgaaagat 
      tacatgcgc caggcgggc gaagtcact tatgccgac 
      gcccataaa cagcaccgt aacgagggc gtcgtcgag 
      ttcgcctcg tactcagac ctcaaaaac gccatcgaa 
      aagctagac gataccgag ctgaacggc agacgcatt 
      cggctgatc gaggatcga aaacgcagt agacgatca 
      ggctcgtac agcagccgt agtcgctcc cgcagccgc 
      tccaggtcc cgaagccgc tccagatct aaaagccgc 
      tccagatcg gccagcgcc aaacccaac agcaagtcc 
      agatccaga tcaaactcg cgcaaaaag tcagtcgat 
      cgctccaga tctaactcg cgcaagaag tcggtcgag 
      agatccaga tcgcgctca aggtcgccc aaacgatca 
      agggaaccg tcgaaggag gcctccagg tccaggtca 
      agatcccca agcaaagaa cgcagcaag tccagatcg 
      cgctctgcc tcacccaac taa
    mRNA sequence
    • >TC012868 RA
      ttgatctcg ttccttttt ggtgtgaaa aaagtggtt 
      aaaacgttt gaaatttgg tcgaaaaag tagcgaggc 
      gttggatct agtcaagtg cgtcgcatg gtggcatcg 
      ctgatagcc gtaggtcaa ggtaaaaaa acgcgtagg 
      gagctaaag ttgatgctg gaagtgccg tcgcatcgg 
      agttgggac gagcgaata aagacagtg ccggcgtac 
      ggtctgtac ctttgcgag gtggagtat tcggatcgg 
      cataggtcc gcgtttaga gagggcttg caagggggc 
      caatagcga tggtagggt ctcgcgtgt acgtcgggg 
      ggttgccgt acgggacca ccgagcggg acttagagc 
      gctttttca ggggctacg ggcgcatgc gggacgtgc 
      tcatcaaaa acggctacg ggtttgttg aatttgatg 
      atcatcgcg atgccgacg atgctgtat atgaattaa 
      acggtaaaa aacttttag gggagcgtg tgactgtcg 
      aacgggccc gagggacac cccgaggtc gagaccaat 
      ggtccagcc gcagcgacc accgctccc atgaacgct 
      acgggcccc ccaccagaa ccaattacc gacttatcg 
      tcgaaaacc tctcgtctc gcattagct ggcaagacc 
      tgaaagatt acatgcgcc aggcgggcg aagtcactt 
      atgccgacg cccataaac agcaccgta acgagggcg 
      tcgtcgagt tcgcctcgt actcagacc tcaaaaacg 
      ccatcgaaa agctagacg ataccgagc tgaacggca 
      gacgcattc ggctgatcg aggatcgaa aacgcagta 
      gacgatcag gctcgtaca gcagccgta gtcgctccc 
      gcagccgct ccaggtccc gaagccgct ccagatcta 
      aaagccgct ccagatcgg ccagcgcca aacccaaca 
      gcaagtcca gatccagat caaactcgc gcaaaaagt 
      cagtcgatc gctccagat ctaactcgc gcaagaagt 
      cggtcgaga gatccagat cgcgctcaa ggtcgccca 
      aacgatcaa gggaaccgt cgaaggagg cctccaggt 
      ccaggtcaa gatccccaa gcaaagaac gcagcaagt 
      ccagatcgc gctctgcct cacccaact aattcttca 
      aacacactt gtagtaaaa agttgtagc ttttagtcc 
      tctatattt tagcattta taaccatat attttgttt 
      aatcataat cgcgttata tacttttgt atataatat 
      tatttgtaa tgagtttct tggacgaat tgcgcacga 
      aaaaacaca ctttgagac ccacgacca cgattttga 
      cacatttgg acggcagga aatttgaac aaagtggtg 
      cagaaagca cacaagtgg ccacagcac aatacggat 
      ttatcgtcg ataataaac cagataacg tcccggcaa 
      aaatcaccg attatctct atttgggat ctcaggatt 
      gttgtgatt caacagttt tgcaaaatt accaaattg 
      aaaacgttc tgagtgtcg gaattcaag tcgagtgtg 
      acagaaatc ataaatacg tcgagtgtt tggatttac 
      cggaaacag acttgggac aagttctag cgcaaagtg 
      ttagtttta tcgagaatg ctgtgcaaa aacagagta 
      atattctag tgcattgta acgccggtg tgagccgat 
      cggcctcca ttgtcatag gttatttaa ttttagttc 
      gtggtttaa attttttcg atgcatata atattgtaa 
      aaaaggcca ggagttgta ttcgaccga atgacggat 
      ttgttacgc aactacgga gtttgacta gatcgtaat 
      tttttcatt ggtttttgt agtttgtag aattgttca 
      ttaaagttt tttattggt atgataaga attaattgt 
      aatttaact tccaataaa gttttatat ttacgtaac 
      ttcattttg tgtaccttt gataacaac attttgtaa 
      ataatcata ggtgcatgg tgtttgaat aaaaaaaca 
      aaggaggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY