Status
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Gene information
General information
TC011614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15467463..15468939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011614
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09538

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011614 RA
      MLGDFDEFF VFWTVLLVV FLCQKYYFQ NSGVIKVND 
      QIVPKSVNY HFTRQCNYE CGFCFHTAK TSFVLDLDV 
      AKKGLRMLK EAGMEKINF SGGEPFIVK RGRYLGEMV 
      KFCKMALKL PSVSIVSNG SLITEKWFQ SYGKYVDIM 
      AVSCDSFNE ETNKLIGRG QGSKNHLEQ ILKVRNWCE 
      KYNVLFKLN TVVNTYNQN EDMCEQVSR INPIRWKVF 
      QCLLLEGEN AGPEALRNA ERFYIDDAT FESFLQRHE 
      SIKSLVPES NTKMQNSYL ILDEYMRFL DCTSGSKVP 
      SKSILDIGV ENALKFSGF DEDMFKKRG GFYKWSKEA 
      ERLSW
    CDS sequence
    • >TC011614 RA
      atgttgggc gattttgac gagtttttc gtattttgg 
      accgtttta ttggtcgtt tttttgtgt cagaagtat 
      tattttcaa aattcgggc gtaataaaa gtgaatgat 
      caaattgta ccgaaaagc gtcaattat cattttacg 
      agacagtgc aattacgag tgcggtttt tgcttccat 
      acggccaaa acgtccttt gttttggat ttggacgtt 
      gccaaaaag gggctaagg atgctcaag gaggctgga 
      atggaaaaa atcaatttt agtggcggg gaacctttt 
      attgtgaaa cgcgggcga tatttgggc gaaatggtc 
      aaattttgc aaaatggct ctgaaacta ccatcggtt 
      tccatagtt tcaaatggg tcgctaatc accgaaaaa 
      tggtttcaa agttatgga aaatacgtc gacattatg 
      gccgtttcg tgcgactct tttaatgag gaaaccaac 
      aagctcatt ggtcgtgga caagggagc aaaaaccac 
      ttggagcaa atcctaaaa gtgcgcaac tggtgcgaa 
      aaatacaac gttttgttc aaattaaac acagtcgtt 
      aacacttac aaccaaaac gaagacatg tgtgaacaa 
      gtcagccga ataaaccca atccgctgg aaagtgttt 
      caatgtttg ttgctcgag ggcgaaaac gccggtcct 
      gaagctttg cgaaacgcc gaacgcttt tacatcgac 
      gatgcaact ttcgaaagc tttctccaa agacatgaa 
      agcatcaaa agtttggtt ccggaatcg aacaccaag 
      atgcagaat agttattta atccttgac gaatacatg 
      cggtttttg gactgtacg agtggctca aaagtgccc 
      tcgaagtca attctggac attggggtt gaaaatgcg 
      ctcaaattt tccggcttt gatgaagac atgttcaag 
      aagagggga ggcttttac aagtggtcc aaggaggcc 
      gagaggctg tcctggtag
    mRNA sequence
    • >TC011614 RA
      ttaatacgt agtagcaac atgttgggc gattttgac 
      gagtttttc gtattttgg accgtttta ttggtcgtt 
      tttttgtgt cagaagtat tattttcaa aattcgggc 
      gtaataaaa gtgaatgat caaattgta ccgaaaagc 
      gtcaattat cattttacg agacagtgc aattacgag 
      tgcggtttt tgcttccat acggccaaa acgtccttt 
      gttttggat ttggacgtt gccaaaaag gggctaagg 
      atgctcaag gaggctgga atggaaaaa atcaatttt 
      agtggcggg gaacctttt attgtgaaa cgcgggcga 
      tatttgggc gaaatggtc aaattttgc aaaatggct 
      ctgaaacta ccatcggtt tccatagtt tcaaatggg 
      tcgctaatc accgaaaaa tggtttcaa agttatgga 
      aaatacgtc gacattatg gccgtttcg tgcgactct 
      tttaatgag gaaaccaac aagctcatt ggtcgtgga 
      caagggagc aaaaaccac ttggagcaa atcctaaaa 
      gtgcgcaac tggtgcgaa aaatacaac gttttgttc 
      aaattaaac acagtcgtt aacacttac aaccaaaac 
      gaagacatg tgtgaacaa gtcagccga ataaaccca 
      atccgctgg aaagtgttt caatgtttg ttgctcgag 
      ggcgaaaac gccggtcct gaagctttg cgaaacgcc 
      gaacgcttt tacatcgac gatgcaact ttcgaaagc 
      tttctccaa agacatgaa agcatcaaa agtttggtt 
      ccggaatcg aacaccaag atgcagaat agttattta 
      atccttgac gaatacatg cggtttttg gactgtacg 
      agtggctca aaagtgccc tcgaagtca attctggac 
      attggggtt gaaaatgcg ctcaaattt tccggcttt 
      gatgaagac atgttcaag aagagggga ggcttttac 
      aagtggtcc aaggaggcc gagaggctg tcctggtag 
      tttttgcat aattttcag tgatgccaa cagtgaagt 
      attaatacg tttaatagt ttgtatcca gtttaactt 
      ttttataat aattaattt gttttggag aaataaacc 
      ttacttaag ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY