Status
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Gene information
General information
TC002076 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6148638..6159504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002076
    iBeetle screen iB_09537

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002076 RA
      MGLRTASAR RPYAEVMAP ALTETSGQK QSFTLPPKY 
      LAPECSDCD IMSDQNMGI HHKTTTSLD LTSPQDVDD 
      LMQVIKSIP EEDMAIENL LTDDDVMNM SIDDPKETL 
      TELKTKQNR IERRLDFLR RKIRKLQAR NMGQHVAGE 
      VAGVYEYVH RSLKRLKDN THLEEEKPQ MKPMSSAST 
      KTMLKKLEM AAVLQAKNA ARQRHTPKY FGSGSCEPG 
      NHRFPAPGV VNVPTWPME NKLELQKVT GLLQTELNL 
      VQHEVDSEA TESSSGGES CDEMQSYNN PHQQYLSIQ 
      KRALWKYAS QRAAIASRW TWLQAQISD LEYRIRQHG 
      DIQRQIRAS KGEIQLGGV SPPPPTSPT AVNGYRGQL 
      PGSSPLTSK SEDVVPTTP NGAHPEYEC ARTRPLVNF 
      KKRKLLQLN GLHVISKKA ARPASVRCG CVNTTAPCA 
      VCTGRTDPT HPRDPTEAL SKPERIALV DPGFHPVFS 
      MPEDASYAV HCEAIMKNP EWQQRSTRM KSLKVLNKD 
      RSDKSVLEH HHRSKKIDH RKKYNRLLK PSTMSALSA 
      KIRNKIRGR KSGRHSTSS LTSFKKRPL LSGGDGADE 
      EVESIGNNS ATGAANSAV GSPSSSPLL HIQAISGYN 
      RRNRANSYD IDNIVIPYS VAAATRVEK LQYKEILTP 
      KWRIADIDL KCDTKNNGA VRNPSQDSD MEDLSEEAV 
      LARHEKSEY EEKKKFLSY LKLPSGHGR SRAHKRTDS 
      LAESSGANT PDPLSPHPA ETAESPLTS PPATPLPEE 
      LPSISAMRR RTISQSRVV PTTVAREEN NTPENVEVA 
      PFDTRTFPL DDETYEKML KSMPENHQF KTNYRAQDC 
      DYLDEKVDS PGSESTESA LGEEDPNDP EWVDMERVR 
      DRHKR
    CDS sequence
    • >TC002076 RA
      atgggattg cgcacggcg tcggcgagg cggccctat 
      gcggaggtc atggcccca gcgttgacg gagaccagc 
      ggccagaag cagagcttc acgttgccc cctaagtac 
      ctcgccccc gagtgctcg gactgtgac attatgagt 
      gaccaaaac atgggcata caccacaaa acgacaacc 
      agcctggac ctaacctcg ccccaggac gtggacgac 
      ctcatgcag gtcatcaaa tccataccg gaggaggat 
      atggccatt gagaatctc ctcaccgat gacgatgtt 
      atgaatatg tcgattgat gaccccaaa gaaacactc 
      accgagttg aagaccaag caaaatcgg atcgaaaga 
      cgactggat tttctcaga aggaagatc aggaagttg 
      caagcgagg aatatggga cagcatgtt gcgggggaa 
      gttgctggg gtctacgag tacgtccac aggtcgctc 
      aagcgcctc aaggacaac acgcacctg gaggaagag 
      aagccccag atgaagcca atgtcgtcg gcgtcgacc 
      aaaaccatg ttaaaaaaa ctggaaatg gctgctgtt 
      cttcaagcg aaaaatgca gcacgtcaa agacataca 
      ccgaaatat ttcggttcg ggttcgtgt gaaccggga 
      aatcaccga tttcccgca cctggtgtt gtaaatgtg 
      ccaacgtgg ccaatggaa aataaactt gaattacaa 
      aaagtgacc ggtctttta caaaccgaa ttaaatcta 
      gtgcaacat gaagttgat tcagaagcc accgagagt 
      agttcgggg ggtgaaagt tgtgatgag atgcagagt 
      tataataat ccgcatcag cagtacctt agtatacaa 
      aaacgtgca ttatggaaa tacgcttct caacgtgcg 
      gccatcgcc tcacgctgg acctggctt caagcccaa 
      atctccgac ttggaatac cgtatacgt cagcacggc 
      gacatccag cgtcaaatt cgcgcatca aagggcgaa 
      attcaactc ggcggcgta agtccgccg ccgccaaca 
      tctccgacg gcggtgaac ggctaccgc ggccaattg 
      ccaggcagt tcgccgctg acgtcgaag agcgaagac 
      gtcgttccg acgacgccg aacggcgcc caccctgaa 
      tacgaatgc gcccgaacg cgccctctc gtcaacttc 
      aagaagcga aaattgttg caattgaac ggcttgcat 
      gtgatttcg aaaaaggcg gcacgtccg gcgtccgta 
      cgctgcgga tgcgtcaat acgacggcg ccgtgcgcc 
      gtttgcacg ggacgcacg gatccgacg catccgcgc 
      gatcccacg gaagcgcta agcaaaccg gaacgcatc 
      gccttggtc gatccgggt tttcatccg gttttttcg 
      atgccggaa gacgcatca tacgctgta cattgcgag 
      gcgattatg aaaaatccc gaatggcaa cagcgcagc 
      acccgaatg aaatctctc aaagttttg aataaggat 
      agatctgac aagtctgtg ttggaacac caccaccga 
      tcgaagaaa atcgaccat cgaaagaaa tacaatcgt 
      ttattgaaa ccgagcacc atgtcggca ctctccgcc 
      aagattcgg aataagata cggggacgt aaaagtgga 
      cgacactcg acttcgtct ctgacgagt tttaagaag 
      cgtccgctg ttaagtggc ggcgatgga gcggacgaa 
      gaagtcgag tctattggt aataatagt gcgacaggg 
      gcggcgaat tcggcggtg ggttcgccg agttcgtca 
      ccgttgctt catatacaa gctatatcg ggttataat 
      cgacggaat cgggcgaat tcgtacgat atcgataat 
      attgttata ccgtatagt gtggcggcg gcgacgcgg 
      gtcgaaaag ttacagtac aaagagatt ttgacacca 
      aaatggcgg atagcagac attgatttg aaatgtgat 
      acgaaaaat aacggagct gttagaaac cctagtcaa 
      gatagcgat atggaagat ctctctgag gaggcggtg 
      ctcgcacgc cacgaaaaa tccgaatac gaagaaaag 
      aaaaagttt ctcagttat ctgaaactg ccgagtggt 
      cacggccgt agccgagcc cacaaacga acggatagt 
      ttagccgaa tcgagcggt gcgaacaca cccgatccg 
      ttatcgccg catccggcg gaaacggcc gaatcgccg 
      ttgacgtca cctccagcc acgcccctt cccgaagag 
      ctcccctcc atcagtgcg atgcgtcgt cggacgatt 
      tcgcagtca cgtgtcgtt ccgacgact gtggcacga 
      gaggagaac aatacgccg gaaaatgtc gaagtggcg 
      ccgtttgac acgcgaacg tttccattg gacgacgaa 
      acgtacgag aagatgctt aaaagtatg ccggaaaat 
      caccagttt aagacgaat tatcgcgcg caggattgt 
      gattatttg gacgagaag gtggactcg cccgggagc 
      gagtccacc gagtcggcg ctgggcgaa gaggacccg 
      aacgatccc gagtgggtg gacatggag agggttaga 
      gacaggcat aagagataa
    mRNA sequence
    • >TC002076 RA
      ttgggtcag atttgagtt tggcgcgcg tagatgtcg 
      ctggcgtcg gttgaatcg tgtcatgtc gtgaagcgc 
      caaaaaagc gtcttgtcg cggtttcgt gcgtttttc 
      gggccggtt ttcggtcgt agcgtgcgt ttaaaaacg 
      gatgtggaa ttattgcgg gtgtttcaa cggtttggg 
      cgcgttgga tttgttacg taatgcgtt ttggttgtt 
      gttttgtct acggcggtg gttgagtcc ttgactgaa 
      atctcgcgt ttgctctgt atcaagtcg cctatggtt 
      gttgtgagt gtaatcgaa tagagtgcg ttgtttggt 
      gtaaagtcg cgtcgccat tgttgtaaa atcgttgta 
      aaatcgtct ttggaggcg gaagtggtg ctggcgatg 
      tgttggtga ttgatttcc ggcggtggt ttggcgtca 
      cgtgcgagt ggttgtgcg gggggcgca atgggggag 
      ctggccggc gactaggac tggagcccc gtttttgga 
      tgaatggga ttgcgcacg gcgtcggcg aggcggccc 
      tatgcggag gtcatggcc ccagcgttg acggagacc 
      agcggccag aagcagagc ttcacgttg ccccctaag 
      tacctcgcc cccgagtgc tcggactgt gacattatg 
      agtgaccaa aacatgggc atacaccac aaaacgaca 
      accagcctg gacctaacc tcgccccag gacgtggac 
      gacctcatg caggtcatc aaatccata ccggaggag 
      gatatggcc attgagaat ctcctcacc gatgacgat 
      gttatgaat atgtcgatt gatgacccc aaagaaaca 
      ctcaccgag ttgaagacc aagcaaaat cggatcgaa 
      agacgactg gattttctc agaaggaag atcaggaag 
      ttgcaagcg aggaatatg ggacagcat gttgcgggg 
      gaagttgct ggggtctac gagtacgtc cacaggtcg 
      ctcaagcgc ctcaaggac aacacgcac ctggaggaa 
      gagaagccc cagatgaag ccaatgtcg tcggcgtcg 
      accaaaacc atgttaaaa aaactggaa atggctgct 
      gttcttcaa gcgaaaaat gcagcacgt caaagacat 
      acaccgaaa tatttcggt tcgggttcg tgtgaaccg 
      ggaaatcac cgatttccc gcacctggt gttgtaaat 
      gtgccaacg tggccaatg gaaaataaa cttgaatta 
      caaaaagtg accggtctt ttacaaacc gaattaaat 
      ctagtgcaa catgaagtt gattcagaa gccaccgag 
      agtagttcg gggggtgaa agttgtgat gagatgcag 
      agttataat aatccgcat cagcagtac cttagtata 
      caaaaacgt gcattatgg aaatacgct tctcaacgt 
      gcggccatc gcctcacgc tggacctgg cttcaagcc 
      caaatctcc gacttggaa taccgtata cgtcagcac 
      ggcgacatc cagcgtcaa attcgcgca tcaaagggc 
      gaaattcaa ctcggcggc gtaagtccg ccgccgcca 
      acatctccg acggcggtg aacggctac cgcggccaa 
      ttgccaggc agttcgccg ctgacgtcg aagagcgaa 
      gacgtcgtt ccgacgacg ccgaacggc gcccaccct 
      gaatacgaa tgcgcccga acgcgccct ctcgtcaac 
      ttcaagaag cgaaaattg ttgcaattg aacggcttg 
      catgtgatt tcgaaaaag gcggcacgt ccggcgtcc 
      gtacgctgc ggatgcgtc aatacgacg gcgccgtgc 
      gccgtttgc acgggacgc acggatccg acgcatccg 
      cgcgatccc acggaagcg ctaagcaaa ccggaacgc 
      atcgccttg gtcgatccg ggttttcat ccggttttt 
      tcgatgccg gaagacgca tcatacgct gtacattgc 
      gaggcgatt atgaaaaat cccgaatgg caacagcgc 
      agcacccga atgaaatct ctcaaagtt ttgaataag 
      gatagatct gacaagtct gtgttggaa caccaccac 
      cgatcgaag aaaatcgac catcgaaag aaatacaat 
      cgtttattg aaaccgagc accatgtcg gcactctcc 
      gccaagatt cggaataag atacgggga cgtaaaagt 
      ggacgacac tcgacttcg tctctgacg agttttaag 
      aagcgtccg ctgttaagt ggcggcgat ggagcggac 
      gaagaagtc gagtctatt ggtaataat agtgcgaca 
      ggggcggcg aattcggcg gtgggttcg ccgagttcg 
      tcaccgttg cttcatata caagctata tcgggttat 
      aatcgacgg aatcgggcg aattcgtac gatatcgat 
      aatattgtt ataccgtat agtgtggcg gcggcgacg 
      cgggtcgaa aagttacag tacaaagag attttgaca 
      ccaaaatgg cggatagca gacattgat ttgaaatgt 
      gatacgaaa aataacgga gctgttaga aaccctagt 
      caagatagc gatatggaa gatctctct gaggaggcg 
      gtgctcgca cgccacgaa aaatccgaa tacgaagaa 
      aagaaaaag tttctcagt tatctgaaa ctgccgagt 
      ggtcacggc cgtagccga gcccacaaa cgaacggat 
      agtttagcc gaatcgagc ggtgcgaac acacccgat 
      ccgttatcg ccgcatccg gcggaaacg gccgaatcg 
      ccgttgacg tcacctcca gccacgccc cttcccgaa 
      gagctcccc tccatcagt gcgatgcgt cgtcggacg 
      atttcgcag tcacgtgtc gttccgacg actgtggca 
      cgagaggag aacaatacg ccggaaaat gtcgaagtg 
      gcgccgttt gacacgcga acgtttcca ttggacgac 
      gaaacgtac gagaagatg cttaaaagt atgccggaa 
      aatcaccag tttaagacg aattatcgc gcgcaggat 
      tgtgattat ttggacgag aaggtggac tcgcccggg 
      agcgagtcc accgagtcg gcgctgggc gaagaggac 
      ccgaacgat cccgagtgg gtggacatg gagagggtt 
      agagacagg cataagaga taatttttt tttcggttc 
      atttttttg ataaagctt gggattttg tattacgag 
      gtctgtgat tgtcattac attacatgt gagatacgt 
      caatacagt tgttgcttt tttagtatt gtaatgtat 
      ttaaaaata cagaattcg aagcttacg tgctaggtt 
      cagttgaca taatttgta atataatca taatttaag 
      taatttctt tttgtgctg ttgcagagt ctagttcac 
      ttgattgta acatgactg gttagacta gttactaat 
      tttaactgt acttttgtt actgaattg gtagttatg 
      ttacttttt tgatgtcaa gtacaaccc agtgacttg 
      ttatatgaa attctattg taaataaag cagtgcaaa 
      tatttgtta tagagatat tttcgaaat catttgcat 
      ttttttcgt ttttatgag aattattca aattgtaca 
      tgctcttca taagagaca aatatgtat catattatt 
      attaaagtg tttattgta aagcaaatc gcgttatat 
      ctttgcaag tattcacca tgtagttac ttcctgtag 
      tattgtacg tttttatat gcagatgta ttgattgaa 
      taaagtcca atctgctta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY