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Gene information
General information
TC012839 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15775760..15777311
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012839
    iBeetle screen iB_09533

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012839 RA
      MRLKFLFFF LTIHLIEAD INPENNACS SIESYHIAQ 
      MSKKCFYNP DVLSDVPTM TARHGYEAK TFTVTTSDG 
      YILTIFRII SNKTEPVNG PVLVQHGIL GSSSSWVAI 
      GNRSLAFYL VDRGYDVWL GNTRGSYYS NQHVNLSVE 
      NPEYWDFDV DTIASIDIP TQLKFVFNN TGEKITYIG 
      HSMGTSVIF MYVASNWDA DNYVKEIIA LAPIAYLND 
      IPIFEFVRP LGLFLVKIL DFVEITGLF YHEDAIHGL 
      LTQICKNTA PELCSLLIS LTSGKTVQF PPDDLLLYY 
      SYWPGGISI YILQQYLQI IQSKQFQKF DYGPKRNAK 
      LYGSQTPPV YNLSEIKLP THLFYGEND IFYRKENIE 
      RLYNEIGSS DKTAFSVGT DEEKPFDHI DFLYSENLI 
      QFLYERMFD NL
    CDS sequence
    • >TC012839 RA
      atgcgttta aaattttta ttctttttc ttgacaatt 
      cacttaatt gaagccgac ataaatcca gaaaacaac 
      gcctgttcc tcaatcgaa tcttaccat attgctcag 
      atgagcaaa aagtgtttt tacaacccg gatgtgtta 
      tcagatgtt cccacaatg accgcaaga catggctat 
      gaagccaaa accttcact gtcacaact agtgatggc 
      tacattttg accattttt cggattatt tcaaacaaa 
      accgaacct gtgaatggg ccagtgttg gtccaacac 
      ggaattttg ggctcgtca tcgtcgtgg gtggcaatt 
      gggaacaga tcacttgcg ttttatcta gttgatcga 
      gggtatgac gtctggttg ggtaatacg agaggttct 
      tactactcc aatcaacac gttaattta tcagtggaa 
      aatccggaa tattgggac tttgacgtg gacactatt 
      gcatccatc gatattccg acacaactc aagttcgtt 
      tttaataac accggtgag aaaatcact tatatcgga 
      cattcgatg ggcacttcg gtcattttt atgtacgtt 
      gcaagcaat tgggacgcc gataattac gttaaagaa 
      attatcgcg cttgcacca attgcctac cttaacgat 
      atcccaata tttgagttt gtgcggcct ttaggcctg 
      tttctggtc aaaatttta gatttcgtc gagatcacc 
      ggtttgttt taccacgag gatgcaatt catggctta 
      ttgacccaa atttgcaaa aacaccgca cctgagctg 
      tgctctctc cttattagc ctcacttcg ggaaaaacc 
      gtccagttc ccacctgac gacctcttg ttgtattac 
      agttattgg ccgggtggc atttccatc tacatactg 
      caacaatat ttgcaaatt attcaaagc aaacaattc 
      caaaaattt gattacggg ccgaaacga aacgccaaa 
      ctttacggt tcacaaaca cctcccgtt tataattta 
      tcggaaatc aagcttccg acgcattta ttttacgga 
      gagaacgat attttttac agaaaagag aatatcgaa 
      cgcctctat aacgaaatc ggaagctcg gacaagacc 
      gctttttca gttgggact gacgaggaa aagcctttc 
      gaccacatt gattttctc tattctgaa aatttaatt 
      caatttttg tacgaacga atgtttgat aatttgtag
    mRNA sequence
    • >TC012839 RA
      tgtcaccaa aatgcgttt aaaattttt attcttttt 
      cttgacaat tcacttaat tgaagccga cataaatcc 
      agaaaacaa cgcctgttc ctcaatcga atcttacca 
      tattgctca gatgagcaa aaagtgttt ttacaaccc 
      ggatgtgtt atcagatgt tcccacaat gaccgcaag 
      acatggcta tgaagccaa aaccttcac tgtcacaac 
      tagtgatgg ctacatttt gaccatttt tcggattat 
      ttcaaacaa aaccgaacc tgtgaatgg gccagtgtt 
      ggtccaaca cggaatttt gggctcgtc atcgtcgtg 
      ggtggcaat tgggaacag atcacttgc gttttatct 
      agttgatcg agggtatga cgtctggtt gggtaatac 
      gagaggttc ttactactc caatcaaca cgttaattt 
      atcagtgga aaatccgga atattggga ctttgacgt 
      ggacactat tgcatccat cgatattcc gacacaact 
      caagttcgt ttttaataa caccggtga gaaaatcac 
      ttatatcgg acattcgat gggcacttc ggtcatttt 
      tatgtacgt tgcaagcaa ttgggacgc cgataatta 
      cgttaaaga aattatcgc gcttgcacc aattgccta 
      ccttaacga tatcccaat atttgagtt tgtgcggcc 
      tttaggcct gtttctggt caaaatttt agatttcgt 
      cgagatcac cggtttgtt ttaccacga ggatgcaat 
      tcatggctt attgaccca aatttgcaa aaacaccgc 
      acctgagct gtgctctct ccttattag cctcacttc 
      gggaaaaac cgtccagtt cccacctga cgacctctt 
      gttgtatta cagttattg gccgggtgg catttccat 
      ctacatact gcaacaata tttgcaaat tattcaaag 
      caaacaatt ccaaaaatt tgattacgg gccgaaacg 
      aaacgccaa actttacgg ttcacaaac acctcccgt 
      ttataattt atcggaaat caagcttcc gacgcattt 
      attttacgg agagaacga tatttttta cagaaaaga 
      gaatatcga acgcctcta taacgaaat cggaagctc 
      ggacaagac cgctttttc agttgggac tgacgagga 
      aaagccttt cgaccacat tgattttct ctattctga 
      aaatttaat tcaattttt gtacgaacg aatgtttga 
      taatttgta gtttgaatt tgcaatttg ggacaagtg 
      gtgtgtttt gaaataaat aattttaac acaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY