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Gene information
General information
TC011634 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15780973..15782518
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011634
    iBeetle screen iB_09532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011634 RA
      MLLKIIFLV HFAHCQTPP QNNVCSNLA DYAFSQLSN 
      NCWYNPDVG STPEEIIKR HGFPFESNQ VITEDGYKL 
      GLFRMPNAG RPVLLQHAF KLYNAGYDV WLGNFRGTI 
      YSNKHNNSN ISEENYWDF SFYEMGIYD LTAMIEFMS 
      KTVGNKRKI IYIGHSMGT TAAFVYAIK RKNHSEKNL 
      EALIALAPV VYMKHVYFP ISGLAPFAQ PIQAVANFL 
      HIHNLGMSN AEKVTNRFN TCLNSLKKL RCEVMTEIM 
      MALNLDSLP PESIPMFMT QAPAGTSVK TLQHYSQLI 
      QNGQFQLFD YGNKNREKY GHEKPPILN PGAVAIPVY 
      LLYGNKDAI GDESDVFEL FGRLGAKKI LKKIPGRDE 
      TNFKHLDFL YGRDINYLY EALFEIIN
    CDS sequence
    • >TC011634 RA
      atgctcctc aaaatcatt tttcttgta cattttgca 
      cattgccaa actccacca caaaataat gtttgttcg 
      aatttagcc gattatgcg ttttcacaa ttgagtaat 
      aattgctgg tataatccc gatgtgggt tcgactccg 
      gaagaaatt ataaaacgg cacggtttt ccgttcgaa 
      tcaaatcaa gtaataacc gaagatgga tacaaattg 
      ggacttttc cggatgccg aacgccgga cgtccggta 
      ttactccag cacgctttc aaactttac aatgcgggc 
      tatgatgtc tggcttggg aatttccgt gggactatt 
      tattcaaat aaacacaat aattccaac atatcggag 
      gaaaattat tgggatttt agtttctac gaaatggga 
      atttacgac ttgacagca atgatcgaa ttcatgtct 
      aaaacagta ggaaataaa cgcaaaatt atttacatt 
      ggacactcg atgggtaca acagccgca ttcgtctat 
      gcaatcaaa cggaaaaat cactctgaa aaaaatttg 
      gaagccttg attgctctc gcacctgtt gtgtacatg 
      aaacacgtt tatttccca atttctggt ctcgcaccc 
      ttcgctcaa ccaatccaa gcagtggcc aatttttta 
      cacatccac aatctcgga atgtcaaat gctgaaaaa 
      gttacaaat cgcttcaac acatgcctt aactccctg 
      aaaaaattg cgatgcgaa gtgatgact gaaataatg 
      atggcttta aacctcgac tctctccca ccggaatca 
      atccccatg tttatgaca caagcacct gccggtact 
      tcggtcaaa acactccag cactattcg caattaatc 
      caaaacggg caattccaa ctgttcgat tatggtaac 
      aaaaatcgg gaaaaatac ggccatgaa aaaccccca 
      atcctgaat ccgggcgca gttgcaatc ccggtttat 
      ttattgtac gggaacaag gacgcaatc ggggacgaa 
      tcggatgtt tttgaattg tttggacgg ctcggggcg 
      aaaaaaatt ctaaagaaa attcccgga cgtgatgaa 
      acgaatttc aaacattta gactttctg tacggccgt 
      gatataaac tatttgtac gaagctttg tttgaaata 
      atcaattaa
    mRNA sequence
    • >TC011634 RA
      gcaaaatgc tcctcaaaa tcatttttc ttgtacatt 
      ttgcacatt gccaaactc caccacaaa ataatgttt 
      gttcgaatt tagccgatt atgcgtttt cacaattga 
      gtaataatt gctggtata atcccgatg tgggttcga 
      ctccggaag aaattataa aacggcacg gttttccgt 
      tcgaatcaa atcaagtaa taaccgaag atggataca 
      aattgggac ttttccgga tgccgaacg ccggacgtc 
      cggtattac tccagcacg ctttcaaac tttacaatg 
      cgggctatg atgtctggc ttgggaatt tccgtggga 
      ctatttatt caaataaac acaataatt ccaacatat 
      cggaggaaa attattggg attttagtt tctacgaaa 
      tgggaattt acgacttga cagcaatga tcgaattca 
      tgtctaaaa cagtaggaa ataaacgca aaattattt 
      acattggac actcgatgg gtacaacag ccgcattcg 
      tctatgcaa tcaaacgga aaaatcact ctgaaaaaa 
      atttggaag ccttgattg ctctcgcac ctgttgtgt 
      acatgaaac acgtttatt tcccaattt ctggtctcg 
      cacccttcg ctcaaccaa tccaagcag tggccaatt 
      ttttacaca tccacaatc tcggaatgt caaatgctg 
      aaaaagtta caaatcgct tcaacacat gccttaact 
      ccctgaaaa aattgcgat gcgaagtga tgactgaaa 
      taatgatgg ctttaaacc tcgactctc tcccaccgg 
      aatcaatcc ccatgttta tgacacaag cacctgccg 
      gtacttcgg tcaaaacac tccagcact attcgcaat 
      taatccaaa acgggcaat tccaactgt tcgattatg 
      gtaacaaaa atcgggaaa aatacggcc atgaaaaac 
      ccccaatcc tgaatccgg gcgcagttg caatcccgg 
      tttatttat tgtacggga acaaggacg caatcgggg 
      acgaatcgg atgtttttg aattgtttg gacggctcg 
      gggcgaaaa aaattctaa agaaaattc ccggacgtg 
      atgaaacga atttcaaac atttagact ttctgtacg 
      gccgtgata taaactatt tgtacgaag ctttgtttg 
      aaataatca attaatttt tgagttgga gttttatcg 
      agggtcact cgaccgaat ataatcaga tgttcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY