Status
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Gene information
General information
TC011644 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15938103..15946718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011644
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011644 RA
      MSKDAKSMI WDYLVFVIF VIASTFVAV YSRFFGPKE 
      KTKADFVFA AGKVSMAAM MLSIARGTL GVRSFLGYP 
      SELFYRGST MWETLYGMV LAYPIVCFI FVPVYFSLG 
      ITSVYQYLD LRFKSRLVR CLASGTYIV RQLLNQGVT 
      VFTPCVALN TVIGIPYWA SIFGITLVS VIFTILGGL 
      KAAILADVM QGLTMIAVS LAIIIQGCI EVGGFGAVF 
      ERNKADGRL DFFNFSMDP TIRVTTTSA LIGQLFMSL 
      SIFGCQQNF VQRYCSMDS QKKVTKTLM CNIPVITVL 
      FSLSWVVGM VVYATYASC DPYTSGYIE KYDEVLPFF 
      VEDRFNYLP GILGLFMAS LFNGALSLN VSNLNSLAT 
      VTFEDFLKP IPFLKGLKD KHQLYTIKA IGVLYGFVI 
      MGISFGVGL LDGVIESSM LVTSATSGP LLGVFIMAL 
      LIPCTNWKG ASTGVIAGH LVTLWITFG RLTIEKPPS 
      PQLPLSTEG CTNTSFNDH ILKPEHQSA YWTFTTTTS 
      APINNNTGN FSLDVINTT RAAPSDPLT QLYSVTYMY 
      YSLIGCFIS ILIGWTVSY FTGSESDLY DQELIHPIA 
      RKMANFFPG KKRRYAEKT IKHKTRRNE SMNHSPSAT 
      SMDKKSGNA NPAFTQDVQ MEVYRTKL
    CDS sequence
    • >TC011644 RA
      atgtcgaaa gacgcaaaa agtatgata tgggactat 
      ctggtcttt gtgattttt gtaatcgca agcaccttt 
      gtcgccgtc tattctcgt ttctttggt cctaaagag 
      aagacaaag gccgatttt gtttttgct gctggcaaa 
      gtttccatg gccgctatg atgctgagt atagcgaga 
      ggaactctc ggtgttcga tcatttttg gggtatccg 
      tccgaattg ttctatcgg ggcagcacg atgtgggaa 
      accctgtac gggatggtc ctggcttat cccatcgta 
      tgtttcatc ttcgtaccg gtgtatttc agcttagga 
      attacctca gtctaccaa tatttggat cttcggttc 
      aaatctcgc ctagtcaga tgtttggca tcgggaact 
      tacatcgta aggcaactc ctaaatcaa ggtgtcaca 
      gtgttcacc ccttgcgtc gccctcaat accgttatc 
      ggaatacct tattgggcc tccatcttc ggcattacc 
      ctcgtcagt gttattttt accatactg ggaggcctg 
      aaagcagca attcttgcc gacgtaatg caaggatta 
      acaatgatc gcagtgagc ctcgcaata atcatccag 
      ggctgtatc gaagtgggt ggtttcggt gcagttttc 
      gaaaggaac aaagccgac ggaagactg gacttcttc 
      aatttcagc atggaccca acgataagg gtaaccacg 
      acatcggca ctgatcggc cagcttttc atgtctttg 
      tcaatattc ggttgccaa cagaacttt gtacaacgc 
      tattgcagc atggattcg cagaaaaaa gtaacaaag 
      actctcatg tgcaacatt cccgtaatc actgtcctg 
      tttagttta tcctgggtt gtgggaatg gtcgtttat 
      gcaacgtat gcttcatgt gatccgtat acttccggt 
      tatatagaa aaatacgat gaagtactt cccttcttt 
      gtagaagat cgtttcaac tacttgcct ggtatactc 
      ggtttgttt atggcttca cttttcaac ggagcgtta 
      agtttgaat gtgtctaat ctgaattct ctggccacc 
      gttacgttc gaagatttc ctcaaaccg attccgttc 
      ttgaaggga ctgaaggac aagcatcag ctgtatact 
      atcaaagct atcggagtt ctctacgga ttcgttatc 
      atgggaatc agcttcgga gtgggcctc ttagacggc 
      gttatcgaa tcatctatg ctggtcacc tccgccact 
      tcaggacct ctcttagga gtcttcata atggccctg 
      ttgataccc tgcaccaac tggaaagga gcttcaact 
      ggcgtcatt gccggtcat ttagttact ctgtggata 
      actttcgga cgattaaca atagaaaaa ccaccatcc 
      ccacagtta cccttatca actgaaggg tgcacaaac 
      acgtccttc aacgatcat attttaaag ccggaacac 
      cagtcggca tactggact tttacgacg acaacttcc 
      gcacccatc aataataac acgggaaac ttttccctc 
      gacgtcatc aacaccaca cgagctgca ccttcggac 
      cctctaact caactctat tcagtgacc tacatgtac 
      tactctctc atcggatgc ttcatttcc atactcatc 
      ggttggaca gtatcctat ttcacgggt agcgaaagc 
      gatttgtac gatcaagaa ttaattcat cccatcgct 
      cgcaagatg gccaacttt ttccctggc aaaaaacga 
      cgctacgca gagaaaacc atcaaacat aaaacacgg 
      agaaatgaa tcgatgaac cattcgcca agtgccacc 
      agtatggat aaaaagagc ggaaatgcg aatcccgct 
      ttcactcaa gacgtacaa atggaagtg tacagaacc 
      aaattatga
    mRNA sequence
    • >TC011644 RA
      ttttttccg cgccaaaaa acaaaaaaa tcccttcgc 
      aaacagtcg ctcgaaaaa tattttttc agtcgtcgc 
      ttaaaagct cttcgattt catggtggg agaatagtt 
      cggtcgcga cctgaccag gagatagtt cgattggta 
      atttttcct cctgtacca tacagtttt caattaatt 
      gtcgcgaat tcttagtga catttgata atcaatagt 
      tttgcgcct gactgtata ccgttcttc gattcaaca 
      cggcccaag agtgcgacc gctggagcc ctcctgacc 
      ttttaccgc ttgctccag gctgaggct gcgattctt 
      taagtacca ccctttatg tttttacta gtttcgtga 
      cggaaccta taaatcaac agatccaag ttggagaga 
      cggcccgac aatgtcgaa agacgcaaa aagtatgat 
      atgggacta tctggtctt tgtgatttt tgtaatcgc 
      aagcacctt tgtcgccgt ctattctcg tttctttgg 
      tcctaaaga gaagacaaa ggccgattt tgtttttgc 
      tgctggcaa agtttccat ggccgctat gatgctgag 
      tatagcgag aggaactct cggtgttcg atcattttt 
      ggggtatcc gtccgaatt gttctatcg gggcagcac 
      gatgtggga aaccctgta cgggatggt cctggctta 
      tcccatcgt atgtttcat cttcgtacc ggtgtattt 
      cagcttagg aattacctc agtctacca atatttgga 
      tcttcggtt caaatctcg cctagtcag atgtttggc 
      atcgggaac ttacatcgt aaggcaact cctaaatca 
      aggtgtcac agtgttcac cccttgcgt cgccctcaa 
      taccgttat cggaatacc ttattgggc ctccatctt 
      cggcattac cctcgtcag tgttatttt taccatact 
      gggaggcct gaaagcagc aattcttgc cgacgtaat 
      gcaaggatt aacaatgat cgcagtgag cctcgcaat 
      aatcatcca gggctgtat cgaagtggg tggtttcgg 
      tgcagtttt cgaaaggaa caaagccga cggaagact 
      ggacttctt caatttcag catggaccc aacgataag 
      ggtaaccac gacatcggc actgatcgg ccagctttt 
      catgtcttt gtcaatatt cggttgcca acagaactt 
      tgtacaacg ctattgcag catggattc gcagaaaaa 
      agtaacaaa gactctcat gtgcaacat tcccgtaat 
      cactgtcct gtttagttt atcctgggt tgtgggaat 
      ggtcgttta tgcaacgta tgcttcatg tgatccgta 
      tacttccgg ttatataga aaaatacga tgaagtact 
      tcccttctt tgtagaaga tcgtttcaa ctacttgcc 
      tggtatact cggtttgtt tatggcttc acttttcaa 
      cggagcgtt aagtttgaa tgtgtctaa tctgaattc 
      tctggccac cgttacgtt cgaagattt cctcaaacc 
      gattccgtt cttgaaggg actgaagga caagcatca 
      gctgtatac tatcaaagc tatcggagt tctctacgg 
      attcgttat catgggaat cagcttcgg agtgggcct 
      cttagacgg cgttatcga atcatctat gctggtcac 
      ctccgccac ttcaggacc tctcttagg agtcttcat 
      aatggccct gttgatacc ctgcaccaa ctggaaagg 
      agcttcaac tggcgtcat tgccggtca tttagttac 
      tctgtggat aactttcgg acgattaac aatagaaaa 
      accaccatc cccacagtt acccttatc aactgaagg 
      gtgcacaaa cacgtcctt caacgatca tattttaaa 
      gccggaaca ccagtcggc atactggac ttttacgac 
      gacaacttc cgcacccat caataataa cacgggaaa 
      cttttccct cgacgtcat caacaccac acgagctgc 
      accttcgga ccctctaac tcaactcta ttcagtgac 
      ctacatgta ctactctct catcggatg cttcatttc 
      catactcat cggttggac agtatccta tttcacggg 
      tagcgaaag cgatttgta cgatcaaga attaattca 
      tcccatcgc tcgcaagat ggccaactt tttccctgg 
      caaaaaacg acgctacgc agagaaaac catcaaaca 
      taaaacacg gagaaatga atcgatgaa ccattcgcc 
      aagtgccac cagtatgga taaaaagag cggaaatgc 
      gaatcccgc tttcactca agacgtaca aatggaagt 
      gtacagaac caaattatg atttttctt tcttcctag 
      agatggatg tgagaaatg gacacggac tcgtgtttt 
      tagtgcctt ttatttggc aacatctgt gatctctag 
      ctgataaga tacaagtgt tcgattgtt gaattatgc 
      aaagactac ttaactatt tttatacct tattacatt 
      gttattatt gaataagat tgtacaact tgatacgcg 
      tacgtgttg aaatattta ttttctttt gtaagctta 
      gactagtgc taacaacga gtatttatt acaacattt 
      tagtacaat aatatttaa ttaataata agcgtagtg 
      tttgttcaa agagtttaa gctaccaac gaaaatata 
      atataaata aaaaaaatt ttgtgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY