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Gene information
General information
TC011647 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15990913..15992757
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011647
    iBeetle screen iB_09527

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011647 RA
      MGKIKAGPV VDILGDEMT RIIWDSIKE KLILPFLDV 
      ELHTYDLGI ENRDKTSDQ VTVDCAEAI KKYNVGIKC 
      ATITPDEKR VEEFNLKQM WKSPNGTIR NILGGTVFR 
      EAIICKNIP RLVSGWNKP IIIGRHAHA DQYKATDFV 
      VPGPGKLEL VFTPISGQP IKHIVNEYK GAGVALGMF 
      NTDESIKDF AHSSFKFAL NQGYPLYLS TKNTILKKY 
      DGRFKDIFQ DIYDREYKK QFEAKGIWY EHRLIDDMV 
      AYAMKSEGG FVWACKNYD GDVQSDSVA QGYGSLGLM 
      TSVLICPDG KTVESEAAH GTVTRHYRM YQKGQETST 
      NPIASIFAW TRGLLHRAK LDNNKELEK FANTLEKVC 
      IDTIESGFM TKDLAICIK GMNGVKRSD YLETFEFMQ 
      KLADNLKKA MGC
    CDS sequence
    • >TC011647 RA
      atgggtaaa ataaaagct ggacctgtt gtcgacatc 
      cttggggat gaaatgacc cgaattata tgggactcc 
      atcaaagag aaattgatt ctgcccttt ttggacgtc 
      gaactgcac acttacgac ctagggatt gaaaaccgg 
      gataaaacc agtgaccag gtcaccgtc gattgtgcc 
      gaagctatc aaaaagtac aatgttgga attaaatgt 
      gccactatt acgcccgat gagaagagg gttgaggag 
      ttcaacttg aaacaaatg tggaagagc ccaaacggc 
      accattaga aacattttg gggggcact gtgttccgc 
      gaggccata atttgcaaa aacatcccc cgattggtg 
      tcgggctgg aacaagcca atcataatc ggtcgtcac 
      gcccacgct gaccaatac aaagccacc gatttcgtg 
      gttcccgga cccgggaaa ctcgaattg gtgtttacg 
      ccaatctcg gggcaaccc atcaaacac atcgtcaac 
      gaatataag ggggctggt gtagccctt ggaatgttc 
      aacaccgac gaatccatt aaagatttc gctcattcg 
      tccttcaaa tttgccctc aatcaagga tatcctctt 
      tacttgagc accaaaaac accattttg aagaaatac 
      gatggacgc ttcaaagac atcttccag gatatctac 
      gatcgcgag tacaagaag caattcgaa gcgaagggc 
      atctggtac gagcatcgt ttgattgac gacatggtc 
      gcgtatgcc atgaaatcg gaagggggc tttgtgtgg 
      gcttgcaag aattatgat ggggacgtt cagtcggat 
      tcagtcgct caaggttat gggtctttg gggctcatg 
      acttcggtt ctgatttgt ccagatggt aaaacagtg 
      gaatcagag gcggctcat ggtactgtc acgcgccat 
      tatcgaatg taccagaag ggacaagaa acgtcaact 
      aatcccatt gcgtccatt ttcgcttgg actagaggg 
      ctgttgcac agagccaag cttgataat aacaaagaa 
      ttggagaag tttgccaac actttggaa aaagtttgc 
      attgatacg atcgaatct ggcttcatg accaaagat 
      ttggccatt tgtattaaa ggcatgaac ggagtcaaa 
      agatccgat tatttggag acgttcgaa tttatgcaa 
      aaattggcc gataatttg aagaaggcc atggggtgt 
      tga
    mRNA sequence
    • >TC011647 RA
      gtttactat aaaaacaca aaaaattac aatctttta 
      tttattatt attaaatta ttaaattat tttttgaca 
      tttattaca caacctcaa tcctcaacc tgtcacctg 
      tcatcctac tgaaaaaca tttaacaaa agcccctcc 
      tctgaaact tgatctttg accaacacc actgtcaac 
      gtcatcgtt gttctgtca atcgccggc cctgaatta 
      attcaattt cgagcaaaa gcaagtcat ttcgtgata 
      aactctcta caatcctaa cataactta gctcctggg 
      tgtttttca ttcacttta aaagttaca agtgtgtgt 
      gtcataaag aaaatgggt aaaataaaa gctggacct 
      gttgtcgac atccttggg gatgaaatg acccgaatt 
      atatgggac tccatcaaa gagaaattg attctgccc 
      tttttggac gtcgaactg cacacttac gacctaggg 
      attgaaaac cgggataaa accagtgac caggtcacc 
      gtcgattgt gccgaagct atcaaaaag tacaatgtt 
      ggaattaaa tgtgccact attacgccc gatgagaag 
      agggttgag gagttcaac ttgaaacaa atgtggaag 
      agcccaaac ggcaccatt agaaacatt ttggggggc 
      actgtgttc cgcgaggcc ataatttgc aaaaacatc 
      ccccgattg gtgtcgggc tggaacaag ccaatcata 
      atcggtcgt cacgcccac gctgaccaa tacaaagcc 
      accgatttc gtggttccc ggacccggg aaactcgaa 
      ttggtgttt acgccaatc tcggggcaa cccatcaaa 
      cacatcgtc aacgaatat aagggggct ggtgtagcc 
      cttggaatg ttcaacacc gacgaatcc attaaagat 
      ttcgctcat tcgtccttc aaatttgcc ctcaatcaa 
      ggatatcct ctttacttg agcaccaaa aacaccatt 
      ttgaagaaa tacgatgga cgcttcaaa gacatcttc 
      caggatatc tacgatcgc gagtacaag aagcaattc 
      gaagcgaag ggcatctgg tacgagcat cgtttgatt 
      gacgacatg gtcgcgtat gccatgaaa tcggaaggg 
      ggctttgtg tgggcttgc aagaattat gatggggac 
      gttcagtcg gattcagtc gctcaaggt tatgggtct 
      ttggggctc atgacttcg gttctgatt tgtccagat 
      ggtaaaaca gtggaatca gaggcggct catggtact 
      gtcacgcgc cattatcga atgtaccag aagggacaa 
      gaaacgtca actaatccc attgcgtcc attttcgct 
      tggactaga gggctgttg cacagagcc aagcttgat 
      aataacaaa gaattggag aagtttgcc aacactttg 
      gaaaaagtt tgcattgat acgatcgaa tctggcttc 
      atgaccaaa gatttggcc atttgtatt aaaggcatg 
      aacggagtc aaaagatcc gattatttg gagacgttc 
      gaatttatg caaaaattg gccgataat ttgaagaag 
      gccatgggg tgttgactc accgcaaga ctgtaatca 
      ccgtacttt tttatattg ttattgcaa ctgctttct 
      ggtgcaggg tgtgtatta tctatgttt tattttaat 
      aaagaacta aagtaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY