Status
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Gene information
General information
TC015971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452070.1: 8395..11821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015971
    iBeetle screen iB_09523

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015971 RA
      MLNVTAAPD TSGATIENL YPALTECFA IILCGYAAG 
      RMNMISETE AKGINTFVG TFSLPSLIF MSLAQLDLS 
      SVNWLFLLA ILIAKSIVF FSVIVVTLL VGRPVNFGR 
      AGIFAIFCT QSNDFAIGY PIVAALYKN SHPEYASYL 
      YLMAPISLA ILNPISFVF MEIGRRNGG GNTDFLLDG 
      DLVSRNMYR FKMVVSVTK SIFLNPIVL MTILGILGN 
      WVFRHHIPC YLGGVLDVL GSAFSASAL FLLGLRMVG 
      KVHKLRGAT LVVPGILIM VKLIALPLV TREVVSIIH 
      VGYNKSETV DLSTYGFLY GTFPSAPTV FVFATQYFI 
      DVDLIASAM VACTFISAP LMFVSAKMI TITKTDPSE 
      YIKQLDSFT LDISIVAII ACGWVILVF ILTKKIARL 
      PHKITACLI ISQLISCLG AVLYNTLPQ KDNWSGYIP 
      SIFIMGVYS TRLWTTILA VTLLFLQCR SLCYVLKLQ 
      PIFFTVGWG IPAILTVVL LLFDGKNHR PLDKRNPNF 
      VYGTYQAIV AIALLVLCF IVTAGCLIL QQRYHRRFT 
      RYLDLAQDI SDSPSTSSE IETNIESPN PGTSSDSGI 
      SPGKSTKCG GLPTINEGC CEESPVDIE DLFPVNGDT 
      GLCPSRFGC QGPKREQCQ GIVRQYQET IDDDLEFIE 
      EEPELHEPQ FLRHTVLLI LLLCSMFVG LAISIWTLV 
      MEQISGIYI ELSFLDATL NFGQSIIVF GIFGLEVKE 
      VVLPLLKLK RLLWYGSNS LVLPSWHEL SPETRHICD 
      QFITHHLQS CRNAIATDK RWRIKVYKS VFTGIEFVD 
      WLITVGLAR DRGEAVNYA RHLIEGKVL KHINGVYHF 
      YDRNLLYTF V
    CDS sequence
    • >TC015971 RA
      atgttgaac gtgacagca gcccccgac acttcgggg 
      gccaccatc gagaatctc taccccgca ttaaccgaa 
      tgtttcgcc ataattcta tgcggttat gccgccggc 
      cgtatgaac atgatttcg gaaacggaa gcgaaagga 
      atcaacact ttcgtgggc acattttcg ctcccatct 
      ctcattttc atgtctctg gcccaactt gacctaagt 
      tccgtgaat tggttgttt ctactggcg atactaatc 
      gccaaaagt attgtgttt ttctcggtg attgtggta 
      acactgctg gttggcaga cctgttaat ttcggacgc 
      gcggggatt tttgcaatt ttttgcacc caaagcaac 
      gacttcgcc attggctat cccatcgtg gcagcactg 
      tacaaaaac agccacccg gagtacgcc tcatacttg 
      tacctaatg gcgccaatc agtcttgcc atcttgaac 
      cccatctcg ttcgttttc atggaaatt gggcgccgg 
      aacggtggt ggtaacact gattttctc cttgatggt 
      gatctggtg tctcgaaac atgtaccgg tttaaaatg 
      gttgtttcc gtaacgaag agtattttc ctgaatccg 
      attgtgtta atgaccatt ttggggatt ttggggaat 
      tgggttttc cgccaccac attccctgt tatttgggg 
      ggcgttttg gacgttcta ggatcagcg ttttcagct 
      agtgcttta tttttgtta ggtttgcga atggttggt 
      aaagtacat aaactacga ggggcaact cttgtagtc 
      cccgggatt ttaattatg gtgaaattg attgcccta 
      cctctagtc actcgtgaa gtggtcagt attattcat 
      gtcgggtat aacaaaagc gaaacggtt gatttgagc 
      acttatggg tttctctac gggacgttc ccgtcagcg 
      cctactgtc tttgttttc gctacacaa tatttcata 
      gatgttgat ctcattgct agtgccatg gtagcttgt 
      acgtttatc agcgccccc ttgatgttt gtttcggca 
      aaaatgata acaatcact aaaacagat ccttcggaa 
      tatatcaaa caactcgat tcttttact ctagatata 
      agcattgtt gccataatt gcgtgcggt tgggtgatt 
      ttagtgttt attcttacg aaaaaaatc gccagattg 
      ccgcacaaa atcaccgct tgcttgatc atatcgcag 
      cttatcagt tgtttgggt gccgtcttg tacaacaca 
      ctaccacag aaagataac tggtcgggt tatatacca 
      tcgattttt atcatgggt gtctatagt acccgccta 
      tggaccaca atccttgcg gtcacttta ctgttttta 
      cagtgtcga agtctctgt tacgtccta aaactacaa 
      ccaattttt ttcacagtc ggttgggga atccccgca 
      attctgaca gttgtttta ttgttattt gacggtaaa 
      aatcaccgc ccacttgat aaacgcaat ccgaatttc 
      gtctacggc acgtatcaa gcaatcgtg gccatagca 
      ctcttagtt ttatgtttc atagttacc gccggctgc 
      ttgattttg caacagcgt taccaccgt cgtttcacc 
      cgctatctt gatttagcc caggatata tccgattcg 
      ccatcaaca agtagcgaa attgagaca aacattgaa 
      agtccgaat ccaggcacg agttccgat tccggtata 
      agtccgggt aaaagcaca aaatgcggg ggtttaccg 
      acgataaac gagggttgt tgtgaggaa agtccggtc 
      gatattgag gatttgttt ccggttaat ggagatacc 
      ggactttgt ccgtcacgt tttggttgt caaggaccg 
      aagcgtgaa caatgccaa gggattgtg agacagtat 
      caggaaact atagacgat gaccttgag tttatcgaa 
      gaggagccg gaattgcac gaaccgcag tttttgaga 
      cacactgta ttgttgatt ctattgttg tgttccatg 
      tttgtgggg ttggcaata tcgatttgg acgcttgtc 
      atggagcaa atttcgggg atttacatc gaattgtcg 
      tttctcgat gcgactttg aatttcggg cagagtatc 
      attgttttc ggtattttt ggtttggag gtgaaagaa 
      gtggttttg ccgctttta aaactgaag agattgctt 
      tggtatgga agtaatagt ttggtgctg ccatcttgg 
      cacgaattg tcacccgag acgcgccat atttgcgac 
      cagtttatc acccaccat ttgcaaagc tgtcgcaat 
      gctattgcg actgataag agatggcgt attaaagtt 
      tataagagt gtcttcacc ggaatcgag ttcgttgat 
      tggttgatt acagtgggg ctggcacgt gacagaggc 
      gaggctgtg aattatgca agacatctg attgaaggc 
      aaagttctg aagcacata aacggggtg taccacttt 
      tacgatagg aatttattg tatactttt gtttag
    mRNA sequence
    • >TC015971 RA
      ttattattc acatcgcaa aatagtgta aaataggtg 
      gttgtgacc ttgaaaagg ccaattatt tgccaaatt 
      ttgctaaaa atttggtca gttgacatg tgtcatctg 
      tcaaaaccc atctgtttt cactcaaat taacgccaa 
      gtgcccccg ttccaatgc cctcgactc caaagtcat 
      tgtcattgt ctaaaccca caccaaaaa tccaataaa 
      acccgataa aaatgtgaa aaaccactc gacacacac 
      aatcatcaa caatgatgt tgaacgtga cagcagccc 
      ccgacactt cgggggcca ccatcgaga atctctacc 
      ccgcattaa ccgaatgtt tcgccataa ttctatgcg 
      gttatgccg ccggccgta tgaacatga tttcggaaa 
      cggaagcga aaggaatca acactttcg tgggcacat 
      tttcgctcc catctctca ttttcatgt ctctggccc 
      aacttgacc taagttccg tgaattggt tgtttctac 
      tggcgatac taatcgcca aaagtattg tgtttttct 
      cggtgattg tggtaacac tgctggttg gcagacctg 
      ttaatttcg gacgcgcgg ggatttttg caatttttt 
      gcacccaaa gcaacgact tcgccattg gctatccca 
      tcgtggcag cactgtaca aaaacagcc acccggagt 
      acgcctcat acttgtacc taatggcgc caatcagtc 
      ttgccatct tgaacccca tctcgttcg ttttcatgg 
      aaattgggc gccggaacg gtggtggta acactgatt 
      ttctccttg atggtgatc tggtgtctc gaaacatgt 
      accggttta aaatggttg tttccgtaa cgaagagta 
      ttttcctga atccgattg tgttaatga ccattttgg 
      ggattttgg ggaattggg ttttccgcc accacattc 
      cctgttatt tggggggcg ttttggacg ttctaggat 
      cagcgtttt cagctagtg ctttatttt tgttaggtt 
      tgcgaatgg ttggtaaag tacataaac tacgagggg 
      caactcttg tagtccccg ggattttaa ttatggtga 
      aattgattg ccctacctc tagtcactc gtgaagtgg 
      tcagtatta ttcatgtcg ggtataaca aaagcgaaa 
      cggttgatt tgagcactt atgggtttc tctacggga 
      cgttcccgt cagcgccta ctgtctttg ttttcgcta 
      cacaatatt tcatagatg ttgatctca ttgctagtg 
      ccatggtag cttgtacgt ttatcagcg cccccttga 
      tgtttgttt cggcaaaaa tgataacaa tcactaaaa 
      cagatcctt cggaatata tcaaacaac tcgattctt 
      ttactctag atataagca ttgttgcca taattgcgt 
      gcggttggg tgattttag tgtttattc ttacgaaaa 
      aaatcgcca gattgccgc acaaaatca ccgcttgct 
      tgatcatat cgcagctta tcagttgtt tgggtgccg 
      tcttgtaca acacactac cacagaaag ataactggt 
      cgggttata taccatcga tttttatca tgggtgtct 
      atagtaccc gcctatgga ccacaatcc ttgcggtca 
      ctttactgt ttttacagt gtcgaagtc tctgttacg 
      tcctaaaac tacaaccaa tttttttca cagtcggtt 
      ggggaatcc ccgcaattc tgacagttg ttttattgt 
      tatttgacg gtaaaaatc accgcccac ttgataaac 
      gcaatccga atttcgtct acggcacgt atcaagcaa 
      tcgtggcca tagcactct tagttttat gtttcatag 
      ttaccgccg gctgcttga ttttgcaac agcgttacc 
      accgtcgtt tcacccgct atcttgatt tagcccagg 
      atatatccg attcgccat caacaagta gcgaaattg 
      agacaaaca ttgaaagtc cgaatccag gcacgagtt 
      ccgattccg gtataagtc cgggtaaaa gcacaaaat 
      gcgggggtt taccgacga taaacgagg gttgttgtg 
      aggaaagtc cggtcgata ttgaggatt tgtttccgg 
      ttaatggag ataccggac tttgtccgt cacgttttg 
      gttgtcaag gaccgaagc gtgaacaat gccaaggga 
      ttgtgagac agtatcagg aaactatag acgatgacc 
      ttgagttta tcgaagagg agccggaat tgcacgaac 
      cgcagtttt tgagacaca ctgtattgt tgattctat 
      tgttgtgtt ccatgtttg tggggttgg caatatcga 
      tttggacgc ttgtcatgg agcaaattt cggggattt 
      acatcgaat tgtcgtttc tcgatgcga ctttgaatt 
      tcgggcaga gtatcattg ttttcggta tttttggtt 
      tggaggtga aagaagtgg ttttgccgc ttttaaaac 
      tgaagagat tgctttggt atggaagta atagtttgg 
      tgctgccat cttggcacg aattgtcac ccgagacgc 
      gccatattt gcgaccagt ttatcaccc accatttgc 
      aaagctgtc gcaatgcta ttgcgactg ataagagat 
      ggcgtatta aagtttata agagtgtct tcaccggaa 
      tcgagttcg ttgattggt tgattacag tggggctgg 
      cacgtgaca gaggcgagg ctgtgaatt atgcaagac 
      atctgattg aaggcaaag ttctgaagc acataaacg 
      gggtgtacc acttttacg ataggaatt tattgtata 
      cttttgttt agcttgttt ggagccaaa gatttattt 
      ttctatgtc cagtgagat acaacgata tgtaaccaa 
      accatactt aggggttgt gattttcgg ttggtaaca 
      ctcatatca gggcttgtg ttagtaatt atttaagtc 
      tgaactaga caatatatg tgtaatgca aatgaaacg 
      ttcacaaat agtttagtt atttattaa caacccgtc 
      cttaagagc tctagtctc gttagtggc gccctcttc 
      cttactccc actccgtcc gatactcac cgtcacact 
      cactaaaaa tgtgcccac atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY