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Gene information
General information
TC011674 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15147435..15148833
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011674
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09521

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011674 RA
      MEFNVYNKC RVCLSADEQ MHNLFDAFD QGITLRDIL 
      ANTTKFEVD KDDGLSPYI CQNCSTIIV DFYNLKTIY 
      EENESQIRA LLASCESQL SENEPQDEP DVITIKVEE 
      SAAPPEPQF EEVDTFTCP ECGESFPDK NAVQSHLVF 
      THNITVIDF DEISEVSLL KITSEKTPS EYKCKICGE 
      IFESGEPYQ QHLETHKKN VCDICGVGF TKKSYLKDH 
      LEVHCPEKR HVCNYCNKA FKRRTVLVK HRRIHTNPR 
      GHICEKCGK AFTDRGTLK THNMLLHVR ERNYVCLIC 
      GVKFPLKAT LEKHIRRHQ NKEREFFCT QCPLGYRDK 
      SSLDRHVFV KHSGRSVKL TCDECGKQY TTMGNLSKH 
      QRVCHRSDE KIE
    CDS sequence
    • >TC011674 RA
      atggaattt aacgtttat aataagtgc cgggtgtgt 
      ctgagcgcc gacgagcag atgcacaac cttttcgac 
      gcgtttgac caaggcatc actttgcgc gatattctg 
      gcaaatacg acaaaattc gaggttgac aaagacgat 
      ggtttgtcc ccctacatt tgccagaac tgctccaca 
      atcatcgtt gatttttat aacctcaaa accatctac 
      gaggagaac gagagtcaa atccgggcc cttttggcc 
      agttgcgag tcgcagttg agcgagaac gagccccaa 
      gacgagccc gacgtcata acaatcaaa gtggaggaa 
      agcgcagcc cccccagag ccccagttc gaagaagtg 
      gacacgttc acgtgcccc gaatgtggc gagtccttc 
      ccggacaaa aacgccgtt cagtcgcac ttggtgttt 
      acgcataac atcacagtg atcgacttt gacgagatt 
      tccgaggtt tcgctcctc aaaatcacg tcagagaag 
      actccaagt gagtacaaa tgcaagatt tgtggcgag 
      atttttgag tcgggggag ccctaccag caacatttg 
      gagacacat aagaagaat gtttgtgat atttgtggt 
      gtcggtttt acgaaaaag tcctacttg aaggaccac 
      ctcgaggtg cattgcccc gaaaaacgc cacgtttgc 
      aactactgc aacaaggcg tttaagcgg aggaccgtt 
      ttggtgaaa cataggcgc atccacacc aacccaagg 
      gggcacatt tgcgaaaag tgcggcaag gctttcaca 
      gatcggggc actttgaag acccacaac atgttgttg 
      cacgtccgt gagcgcaat tatgtgtgt ctgatatgt 
      ggggtgaag ttcccgctg aaagcgacg cttgagaag 
      cacatccgg aggcaccag aataaggaa cgcgagttt 
      ttttgcacg cagtgtccc ctggggtac agggacaag 
      tcgagcttg gacagacat gtgtttgtt aagcacagt 
      ggacggagt gttaagttg acgtgtgat gagtgcggc 
      aagcagtat acgacaatg ggcaatttg tccaagcat 
      caaagggtc tgtcataga agcgatgaa aaaattgag 
      tga
    mRNA sequence
    • >TC011674 RA
      gtttaaagg tgtaaataa tcattccgg gatggaatt 
      taacgttta taataagtg ccgggtgtg tctgagcgc 
      cgacgagca gatgcacaa ccttttcga cgcgtttga 
      ccaaggcat cactttgcg cgatattct ggcaaatac 
      gacaaaatt cgaggttga caaagacga tggtttgtc 
      cccctacat ttgccagaa ctgctccac aatcatcgt 
      tgattttta taacctcaa aaccatcta cgaggagaa 
      cgagagtca aatccgggc ccttttggc cagttgcga 
      gtcgcagtt gagcgagaa cgagcccca agacgagcc 
      cgacgtcat aacaatcaa agtggagga aagcgcagc 
      ccccccaga gccccagtt cgaagaagt ggacacgtt 
      cacgtgccc cgaatgtgg cgagtcctt cccggacaa 
      aaacgccgt tcagtcgca cttggtgtt tacgcataa 
      catcacagt gatcgactt tgacgagat ttccgaggt 
      ttcgctcct caaaatcac gtcagagaa gactccaag 
      tgagtacaa atgcaagat ttgtggcga gatttttga 
      gtcggggga gccctacca gcaacattt ggagacaca 
      taagaagaa tgtttgtga tatttgtgg tgtcggttt 
      tacgaaaaa gtcctactt gaaggacca cctcgaggt 
      gcattgccc cgaaaaacg ccacgtttg caactactg 
      caacaaggc gtttaagcg gaggaccgt tttggtgaa 
      acataggcg catccacac caacccaag ggggcacat 
      ttgcgaaaa gtgcggcaa ggctttcac agatcgggg 
      cactttgaa gacccacaa catgttgtt gcacgtccg 
      tgagcgcaa ttatgtgtg tctgatatg tggggtgaa 
      gttcccgct gaaagcgac gcttgagaa gcacatccg 
      gaggcacca gaataagga acgcgagtt tttttgcac 
      gcagtgtcc cctggggta cagggacaa gtcgagctt 
      ggacagaca tgtgtttgt taagcacag tggacggag 
      tgttaagtt gacgtgtga tgagtgcgg caagcagta 
      tacgacaat gggcaattt gtccaagca tcaaagggt 
      ctgtcatag aagcgatga aaaaattga gtgattgtt 
      gtagaagaa actgtatat aataataaa aaaatcgag 
      aataaaaag ttttttatt ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY