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Gene information
General information
TC011679 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15124975..15126393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011679
    iBeetle screen iB_09518

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011679 RA
      MESLSQTLQ PHKDTVGTV ASYLTILQF 
FSGVFICRD IYKKGNTDG VNSMPFVGG 
IMLGLAMLK YGLMLGDEN MLLVNLFAI 
VLNVIYCIV YYFYSNDKW KQILKPLSI 
SMAFVAVLW GYCEYESPS VVEFRYGLI 
VTILMLAVL GSPLLGVKE IIEKKDASE 
IPFVLTLMA TLVTFSWLL YAIILKNEF 
MLVQNVAGF VLCFVQLIL IFAYPGGGR 
QVSKKKKKH
    CDS sequence
    • >TC011679 RA
      atggagtca ctctcgcag acgctacag ccccacaaa 
gacaccgtg gggacggtg gccagttac ctcacaatt 
ctgcaattt ttctcgggg gttttcatt tgtagagac 
atttacaaa aaaggcaac actgacggt gtgaattcg 
atgccgttt gtgggcggt atcatgtta gggttggca 
atgctgaaa tatggtttg atgcttggg gacgaaaac 
atgctgcta gtaaactta ttcgcaatc gtccttaac 
gtaatttac tgcatcgtc tattatttt tactcgaat 
gacaagtgg aagcaaatc ctgaagccc ctctccata 
agcatggct tttgttgcg gtcttgtgg ggctattgc 
gagtatgag agcccctca gtggtcgaa tttcgctac 
ggattgatt gtcacaata ctgatgctt gccgttttg 
gggagcccc cttctcgga gttaaagaa atcatcgag 
aaaaaagac gcctctgaa atccccttc gttctaaca 
ttaatggcg actttggtg accttctcg tggcttttg 
tacgccatt attttaaaa aatgaattc atgcttgtt 
caaaacgtg gctgggttt gtgctgtgt ttcgtccag 
ttaatttta atttttgct tatccagga ggcgggaga 
caagtaagc aaaaaaaag aagaagcat taa
    mRNA sequence
    • >TC011679 RA
      aaggggacg aaggtcaca gtcaaggac atgacgaga 
agatcatta attcttcat tattgcgag cggcaatct 
caattaaac gattcctgg attgattat tgcatccag 
tactaaaaa ttgtaatca ctacatttt caaaagtgg 
atttatttg cttgtcatc gtgtcaagg cttaacagt 
aaattgcga ttagataat aaaatatct tataacact 
gctgcgaat tcgtaaacg gcgagtatt gtctccttg 
ttgtttaat ttggaagaa ggggttcaa atgaatttg 
acctcaatc aacctctag tgcagtgat aaataccac 
acttttgtt tttcaggtt gtttagttc gcaatggag 
tcactctcg cagacgcta cagccccac aaagacacc 
gtggggacg gtggccagt tacctcaca attctgcaa 
tttttctcg ggggttttc atttgtaga gacatttac 
aaaaaaggc aacactgac ggtgtgaat tcgatgccg 
tttgtgggc ggtatcatg ttagggttg gcaatgctg 
aaatatggt ttgatgctt ggggacgaa aacatgctg 
ctagtaaac ttattcgca atcgtcctt aacgtaatt 
tactgcatc gtctattat ttttactcg aatgacaag 
tggaagcaa atcctgaag cccctctcc ataagcatg 
gcttttgtt gcggtcttg tggggctat tgcgagtat 
gagagcccc tcagtggtc gaatttcgc tacggattg 
attgtcaca atactgatg cttgccgtt ttggggagc 
ccccttctc ggagttaaa gaaatcatc gagaaaaaa 
gacgcctct gaaatcccc ttcgttcta acattaatg 
gcgactttg gtgaccttc tcgtggctt ttgtacgcc 
attatttta aaaaatgaa ttcatgctt gttcaaaac 
gtggctggg tttgtgctg tgtttcgtc cagttaatt 
ttaattttt gcttatcca ggaggcggg agacaagta 
agcaaaaaa aagaagaag cattaaaaa agggcaaac 
aattaaaga attaagtat tgaataatt cacattcca 
ggcagcggc ggcagcgag ctgagtttt cgccaaatg 
ttattttca acgccttag ttgaataaa tcattcaaa 
ttaaa
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