Status
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Gene information
General information
TC012804 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15122505..15124365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012804
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09517

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012804 RA
      MLQFWRKSK AGNDTKCAA PAVTPRPRP PKPPPLDLD 
      IDEFEATRS RLCRTLDEV LGDKGALGY FTQFMEARN 
      AAKFINCWG DIESFKCEI STDCDSSDT QNSSDSCES 
      SLVEGAVAI FKKYIAQES AHNVKCPES VRNELVENI 
      CDLEKIASC LDPVQSFVT EVMEKEYFE AFLASDYFC 
      KYQIDVLTS GQVALDDIL YNETALFYF MEFLEQENS 
      CNLLEFWIA ATNLQQHLT NQKEFFDPI EAQNDAVVL 
      YDKYFSLQA HCPLGFGDK VRFEIEQNI CGENGLVLD 
      CFKLPLKIV EQVLEKNYL KPFLESQLF CKYLSELIN 
      TVQSNDYFN LENNRTLSS DCSSEKSFS TNSAFLALE 
      LNFNNNNCK KKDKNADMS IDTRQLCDP DSLWKRRRT 
      HRLSCGRIT ALGKFETDL EPEPDRKSF KFKNVVKKF 
      VSLDEDKRK EEMAWQVAE MIVKDITNV TLNQEKPSS 
      SL
    CDS sequence
    • >TC012804 RA
      atgttacag ttttggagg aaatcaaaa gccggcaat 
      gacacgaaa tgcgcagcc ccagcggtg acgccgcgg 
      ccccggccc ccgaagccg ccccctctg gacctcgat 
      atcgatgag tttgaggca acgaggtca cggctatgt 
      cggaccttg gacgaggtt ttgggcgat aagggggct 
      ctgggctac tttacccag tttatggag gcgcgaaac 
      gccgccaaa tttatcaat tgttggggg gacattgag 
      tcgtttaag tgtgagata agtacggat tgcgactcc 
      agtgacacc caaaattcg agtgattcg tgtgaatct 
      agtctagtt gagggcgct gttgcgatt tttaaaaag 
      tacatagct caggagtcc gctcacaat gttaaatgc 
      cccgagtca gttcgtaac gaactcgtt gaaaacatt 
      tgtgacttg gagaaaatc gccagttgt ttggacccc 
      gttcagagt ttcgttacc gaagtcatg gagaaggag 
      tattttgag gcgttttta gccagcgat tatttctgc 
      aaataccag atcgatgtt ttgacgagt ggccaagtg 
      gcccttgac gatattctc tacaacgaa acggcgctt 
      ttctacttt atggagttt ctcgaacag gagaattcg 
      tgcaatttg ttggagttc tggatcgct gccaccaac 
      ttgcagcaa catttgacc aatcaaaag gagtttttt 
      gacccaatt gaggcccaa aatgacgct gttgtgctc 
      tacgataag tatttttcg ctgcaagcc cattgtccg 
      ttgggtttc ggcgataaa gttcgcttc gaaatcgaa 
      caaaatatt tgcggggag aacggactt gtcttggat 
      tgtttcaag ttgcccttg aaaatcgtc gaacaagtg 
      ttggagaaa aactatctc aaaccgttt ttggagtca 
      cagttattt tgcaaatat ctgtccgag ttgataaac 
      accgttcag tctaacgat tattttaat ctcgagaat 
      aatcggacg ctttcgtcc gattgcagc agcgagaag 
      agtttcagt acaaatagc gcatttcta gcgttagag 
      ttaaacttt aataataat aattgtaaa aagaaggat 
      aaaaacgca gatatgagt atcgacacg aggcagttg 
      tgcgacccc gattcgctc tggaagagg agacgaacg 
      cacagatta tcctgtggg aggatcaca gctttgggt 
      aaatttgaa actgatttg gagccagaa cctgatcgg 
      aaaagtttc aagtttaaa aatgttgtc aagaagttt 
      gtgagtctc gacgaggat aaaaggaag gaagaaatg 
      gcctggcaa gtggccgaa atgatcgtc aaagatatt 
      acaaatgtt actctaaat caggaaaaa ccatcatct 
      tcattgtga
    mRNA sequence
    • >TC012804 RA
      tgtgccgtg aatcacaat caactttgt ttatcaaaa 
      caatcggtt ttgtgggtg ttttggggc ctcttggca 
      ctgttgggt tgatcggac tcgcccccg cccccacaa 
      cccatcatt acgatgaac catgttaca gttttggag 
      gaaatcaaa agccggcaa tgacacgaa atgcgcagc 
      cccagcggt gacgccgcg gccccggcc cccgaagcc 
      gccccctct ggacctcga tatcgatga gtttgaggc 
      aacgaggtc acggctatg tcggacctt ggacgaggt 
      tttgggcga taagggggc tctgggcta ctttaccca 
      gtttatgga ggcgcgaaa cgccgccaa atttatcaa 
      ttgttgggg ggacattga gtcgtttaa gtgtgagat 
      aagtacgga ttgcgactc cagtgacac ccaaaattc 
      gagtgattc gtgtgaatc tagtctagt tgagggcgc 
      tgttgcgat ttttaaaaa gtacatagc tcaggagtc 
      cgctcacaa tgttaaatg ccccgagtc agttcgtaa 
      cgaactcgt tgaaaacat ttgtgactt ggagaaaat 
      cgccagttg tttggaccc cgttcagag tttcgttac 
      cgaagtcat ggagaagga gtattttga ggcgttttt 
      agccagcga ttatttctg caaatacca gatcgatgt 
      tttgacgag tggccaagt ggcccttga cgatattct 
      ctacaacga aacggcgct tttctactt tatggagtt 
      tctcgaaca ggagaattc gtgcaattt gttggagtt 
      ctggatcgc tgccaccaa cttgcagca acatttgac 
      caatcaaaa ggagttttt tgacccaat tgaggccca 
      aaatgacgc tgttgtgct ctacgataa gtatttttc 
      gctgcaagc ccattgtcc gttgggttt cggcgataa 
      agttcgctt cgaaatcga acaaaatat ttgcgggga 
      gaacggact tgtcttgga ttgtttcaa gttgccctt 
      gaaaatcgt cgaacaagt gttggagaa aaactatct 
      caaaccgtt tttggagtc acagttatt ttgcaaata 
      tctgtccga gttgataaa caccgttca gtctaacga 
      ttattttaa tctcgagaa taatcggac gctttcgtc 
      cgattgcag cagcgagaa gagtttcag tacaaatag 
      cgcatttct agcgttaga gttaaactt taataataa 
      taattgtaa aaagaagga taaaaacgc agatatgag 
      tatcgacac gaggcagtt gtgcgaccc cgattcgct 
      ctggaagag gagacgaac gcacagatt atcctgtgg 
      gaggatcac agctttggg taaatttga aactgattt 
      ggagccaga acctgatcg gaaaagttt caagtttaa 
      aaatgttgt caagaagtt tgtgagtct cgacgagga 
      taaaaggaa ggaagaaat ggcctggca agtggccga 
      aatgatcgt caaagatat tacaaatgt tactctaaa 
      tcaggaaaa accatcatc ttcattgtg ataatgtca 
      gagagctca aaaatgttg tatgtacgg cggcgtgtg 
      atagaaact tcccacttt ttttcaaag gctgtgttt 
      ccccccgtg aattgagtt ggtagcgct gcgatcgga 
      tggctctgt aggttagtc attggattt tattatttt 
      tgtatgatt gcatttgtt tgtaaatac ttctttaat 
      gtataaaca tatttatat aataaaata ttttaacta 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY