Status
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Gene information
General information
TC011686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15097457..15103173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011686
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09516

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011686 RA
      MASLSLPRV LHPRKTSLE IERENFEKS QAQALSKAI 
      NSQETPVKQ KHVRSAIIG TFHTQGATT FWSIALKLP 
      AMDDRIVAW KLCHVVHKI LREGHPLCL VHSQRHRHD 
      LDEIGKLWV HLKEGYGKM IRLYTSLLM TKLDFHRKN 
      PRFPGHLGV SHEELESIG GNDINNYFQ MTVEMFDYL 
      DNIIDLQAA VFGSLDMAR SNSMTSAGQ CRLAPLIPC 
      IQDASKLYD YCVKILFKL HFALPPDLL SGHRQRFLK 
      QFKQLKSFY QNTSNLQYF KDLIAIPPL PENPPNFLI 
      QSDLRTYVT QEVVVPQEP EEEHVTEGS LIDTSETSD 
      FTDNHSRNS ISPSPAHQE ILVERDFLK SQCDKLSSD 
      IGILLARNQ KIVDDYQTK ITNLETELA TKESQLLQE 
      RQVKEDLMN QAFAVAQSQ DCEQKVKDE KFNKLKEVY 
      VKLRNDHIQ KLREMAEAG KKVNQLTQQ VSSIEQMKV 
      ELEATIVSL REQVAKSDE KLQKSANSQ NEELEALKR 
      DKELFNMET EILKKSIDD ACKERDGLM VELREMRAK 
      NEGLLREVE EWSEKFKVV EEESETVRK TSEDEIRKI 
      VLQSLENNE LTLKQALDE VDNPALTAL SCSPDYLRS 
      LSKVCLESL KYEIDLIKT TNSYVVVKA NEITHRICV 
      FVIQGRATG NNSPDINFG EKMAEECKI LGGIILKLL 
      QCFKERSDT KSLQNEAIV KLEEVAALA DNINVSMNG 
      IKAENLADL LESEMMEMD KAIEEAANR IQDMLTKSR 
      TADSGIKLE VNSKLLDSC TTLMQAIRV LVQKSRLLQ 
      AEIVAQGKG TASAKEFYK RNHQWTDGF ISAAKAVAV 
      AAKFLLTAA DKVVSSNGK LEQMVVAAQ EIAASTAQL 
      VVASRVKAN RNSANLQQL TQASKGVTT ATGTVVATV 
      KDCSQLIDE NEELDTSNL TLHQAKTLE MESQVRVLE 
      LEKELEQER HRLLALRKH HYKLEGDNE N
    CDS sequence
    • >TC011686 RA
      atggcctcg ttgtctttg ccccgcgtc ctgcacccc 
      cggaaaacc agtcttgaa attgaacgc gaaaatttc 
      gaaaaatcg caggcccaa gccctctcg aaggcgatc 
      aacagccag gaaaccccc gtaaaacaa aaacatgtc 
      cgttcggcc atcatcggt acgtttcac acacaaggt 
      gcgacgact ttttggtcg atcgccctt aaactaccg 
      gcgatggac gaccgaatc gtcgcttgg aagttgtgt 
      catgttgtt cataaaatc ctccgagag ggtcaccct 
      ttgtgcctt gtccacagc caacgacat cgtcacgat 
      ctcgacgaa atcggaaaa ttatgggtg catttgaaa 
      gagggttac ggtaagatg attcggttg tatacgagt 
      cttctgatg acgaagttg gattttcat cggaaaaat 
      ccgcgtttt cctggccat ttgggcgtt tcgcacgag 
      gagttggag tcgattggt gggaacgat attaataat 
      tattttcaa atgacggtg gaaatgttt gattatttg 
      gataatata attgacttg caagctgcc gtttttggg 
      tcgcttgat atggcgcgc tcgaattcg atgaccagt 
      gctggacaa tgtcggttg gcaccactg attccttgt 
      atccaagat gcttccaaa ttgtacgat tattgcgtg 
      aaaatcttg ttcaagcta cattttgcc ttaccgcct 
      gatttactc tcaggtcat agacagagg tttctcaaa 
      cagtttaaa cagttgaaa tcgttttat caaaatacg 
      agcaatttg caatacttt aaagacttg attgcgatt 
      ccgccacta cctgagaat cctccgaat tttttaata 
      cagagtgat cttcgtaca tacgtaaca caagaagtt 
      gtagtaccg caggaaccg gaagaggaa catgtgact 
      gaaggtagt cttattgat acgtccgaa acgtcggat 
      tttacggat aatcacagt cggaattcg atttctcca 
      agtccggca catcaggaa attttagtc gaacgagat 
      tttctcaaa agtcagtgc gacaaattg agttccgat 
      atcggtatt ttgttggct cgtaatcaa aaaatcgtc 
      gatgattac caaacgaaa atcaccaat cttgagacc 
      gaattagcg acaaaagaa agtcaattg cttcaagag 
      cgtcaagtg aaggaagat ttaatgaat caggctttt 
      gctgtggca cagtcacag gattgtgaa cagaaagtt 
      aaagacgaa aaattcaat aaattaaaa gaggtgtat 
      gtgaaatta cgcaacgat catattcaa aaattacgt 
      gaaatggcc gaagccgga aaaaaagtt aatcaatta 
      acgcagcaa gtctcatca attgaacaa atgaaagtt 
      gagcttgaa gcaacaata gtgtcatta cgggaacaa 
      gtagcaaaa tcggatgaa aaattacag aaatctgca 
      aattctcaa aatgaggaa ttggaagct ttgaaacgt 
      gataaagaa ttgtttaat atggagacg gaaattttg 
      aaaaaaagc atcgatgat gcgtgcaaa gaacgcgat 
      ggtttgatg gtcgaattg agggaaatg cgtgcgaaa 
      aatgagggt ttgttgagg gaggttgag gaatggagt 
      gagaaattc aaggttgtg gaggaggag agtgagaca 
      gttaggaaa acttcagag gacgagata cgaaaaatc 
      gtcttgcaa tcgctcgaa aacaacgaa ctaacccta 
      aaacaagcc cttgacgag gttgataac cctgcccta 
      actgcgctc tcgtgcagt cccgattat ttacgaagt 
      ttgagtaaa gtttgttta gagtcgtta aaatacgaa 
      attgatttg atcaaaaca acgaattcg tacgtagtt 
      gttaaagcg aatgaaata acgcatcgg atttgtgtg 
      tttgttatt caaggacgg gcgacgggt aacaatagt 
      cctgatatt aattttggc gaaaaaatg gcagaagag 
      tgcaaaatt ttgggtgga attatactc aaacttctt 
      cagtgtttt aaagagcgg tctgataca aagtcgctt 
      caaaacgag gcgattgtt aaacttgaa gaagttgca 
      gcacttgct gataatatt aacgtttca atgaatggg 
      attaaggcc gaaaatttg gccgatttg ctggaaagc 
      gaaatgatg gaaatggat aaagcgatc gaggaagcg 
      gccaatcgt attcaggac atgttgacg aaatcacgg 
      accgccgat tctggcata aaactcgaa gttaatagc 
      aaattactc gactcttgc acgactcta atgcaggca 
      attagagtc ctagtgcag aaatcgcgt ttattacaa 
      gccgaaatc gtagctcaa ggaaaagga accgcaagt 
      gcgaaagaa ttttacaaa cgcaaccat caatggacc 
      gatggtttt atttcggcg gcgaaagcc gtggcagtc 
      gcagcgaaa tttctttta acagcggct gataaagtg 
      gtttcatca aacggtaaa ttagaacaa atggttgtg 
      gcggcgcaa gaaatcgcc gcctcgacg gcgcaattg 
      gttgtcgca agtcgggtc aaagccaat cgaaatagc 
      gcaaatttg cagcaattg acgcaagct tccaaaggc 
      gtgaccaca gccacagga acggttgtg gcgacggtt 
      aaggactgc agtcagttg attgatgaa aatgaagag 
      ttggatacg tcgaatttg acactgcat caagcaaag 
      acgctcgag atggaatct caagtgagg gttttggag 
      cttgaaaag gagttggaa caggagagg catcggttg 
      ttggccttg aggaagcac cattataag ctcgaagga 
      gataatgaa aattga
    mRNA sequence
    • >TC011686 RA
      agttttgtg aaattaaat gtgagttgt gcttgttta 
      gagattaaa taatttgtt aaatgagtg taattttgt 
      tataaaact atggcctcg ttgtctttg ccccgcgtc 
      ctgcacccc cggaaaacc agtcttgaa attgaacgc 
      gaaaatttc gaaaaatcg caggcccaa gccctctcg 
      aaggcgatc aacagccag gaaaccccc gtaaaacaa 
      aaacatgtc cgttcggcc atcatcggt acgtttcac 
      acacaaggt gcgacgact ttttggtcg atcgccctt 
      aaactaccg gcgatggac gaccgaatc gtcgcttgg 
      aagttgtgt catgttgtt cataaaatc ctccgagag 
      ggtcaccct ttgtgcctt gtccacagc caacgacat 
      cgtcacgat ctcgacgaa atcggaaaa ttatgggtg 
      catttgaaa gagggttac ggtaagatg attcggttg 
      tatacgagt cttctgatg acgaagttg gattttcat 
      cggaaaaat ccgcgtttt cctggccat ttgggcgtt 
      tcgcacgag gagttggag tcgattggt gggaacgat 
      attaataat tattttcaa atgacggtg gaaatgttt 
      gattatttg gataatata attgacttg caagctgcc 
      gtttttggg tcgcttgat atggcgcgc tcgaattcg 
      atgaccagt gctggacaa tgtcggttg gcaccactg 
      attccttgt atccaagat gcttccaaa ttgtacgat 
      tattgcgtg aaaatcttg ttcaagcta cattttgcc 
      ttaccgcct gatttactc tcaggtcat agacagagg 
      tttctcaaa cagtttaaa cagttgaaa tcgttttat 
      caaaatacg agcaatttg caatacttt aaagacttg 
      attgcgatt ccgccacta cctgagaat cctccgaat 
      tttttaata cagagtgat cttcgtaca tacgtaaca 
      caagaagtt gtagtaccg caggaaccg gaagaggaa 
      catgtgact gaaggtagt cttattgat acgtccgaa 
      acgtcggat tttacggat aatcacagt cggaattcg 
      atttctcca agtccggca catcaggaa attttagtc 
      gaacgagat tttctcaaa agtcagtgc gacaaattg 
      agttccgat atcggtatt ttgttggct cgtaatcaa 
      aaaatcgtc gatgattac caaacgaaa atcaccaat 
      cttgagacc gaattagcg acaaaagaa agtcaattg 
      cttcaagag cgtcaagtg aaggaagat ttaatgaat 
      caggctttt gctgtggca cagtcacag gattgtgaa 
      cagaaagtt aaagacgaa aaattcaat aaattaaaa 
      gaggtgtat gtgaaatta cgcaacgat catattcaa 
      aaattacgt gaaatggcc gaagccgga aaaaaagtt 
      aatcaatta acgcagcaa gtctcatca attgaacaa 
      atgaaagtt gagcttgaa gcaacaata gtgtcatta 
      cgggaacaa gtagcaaaa tcggatgaa aaattacag 
      aaatctgca aattctcaa aatgaggaa ttggaagct 
      ttgaaacgt gataaagaa ttgtttaat atggagacg 
      gaaattttg aaaaaaagc atcgatgat gcgtgcaaa 
      gaacgcgat ggtttgatg gtcgaattg agggaaatg 
      cgtgcgaaa aatgagggt ttgttgagg gaggttgag 
      gaatggagt gagaaattc aaggttgtg gaggaggag 
      agtgagaca gttaggaaa acttcagag gacgagata 
      cgaaaaatc gtcttgcaa tcgctcgaa aacaacgaa 
      ctaacccta aaacaagcc cttgacgag gttgataac 
      cctgcccta actgcgctc tcgtgcagt cccgattat 
      ttacgaagt ttgagtaaa gtttgttta gagtcgtta 
      aaatacgaa attgatttg atcaaaaca acgaattcg 
      tacgtagtt gttaaagcg aatgaaata acgcatcgg 
      atttgtgtg tttgttatt caaggacgg gcgacgggt 
      aacaatagt cctgatatt aattttggc gaaaaaatg 
      gcagaagag tgcaaaatt ttgggtgga attatactc 
      aaacttctt cagtgtttt aaagagcgg tctgataca 
      aagtcgctt caaaacgag gcgattgtt aaacttgaa 
      gaagttgca gcacttgct gataatatt aacgtttca 
      atgaatggg attaaggcc gaaaatttg gccgatttg 
      ctggaaagc gaaatgatg gaaatggat aaagcgatc 
      gaggaagcg gccaatcgt attcaggac atgttgacg 
      aaatcacgg accgccgat tctggcata aaactcgaa 
      gttaatagc aaattactc gactcttgc acgactcta 
      atgcaggca attagagtc ctagtgcag aaatcgcgt 
      ttattacaa gccgaaatc gtagctcaa ggaaaagga 
      accgcaagt gcgaaagaa ttttacaaa cgcaaccat 
      caatggacc gatggtttt atttcggcg gcgaaagcc 
      gtggcagtc gcagcgaaa tttctttta acagcggct 
      gataaagtg gtttcatca aacggtaaa ttagaacaa 
      atggttgtg gcggcgcaa gaaatcgcc gcctcgacg 
      gcgcaattg gttgtcgca agtcgggtc aaagccaat 
      cgaaatagc gcaaatttg cagcaattg acgcaagct 
      tccaaaggc gtgaccaca gccacagga acggttgtg 
      gcgacggtt aaggactgc agtcagttg attgatgaa 
      aatgaagag ttggatacg tcgaatttg acactgcat 
      caagcaaag acgctcgag atggaatct caagtgagg 
      gttttggag cttgaaaag gagttggaa caggagagg 
      catcggttg ttggccttg aggaagcac cattataag 
      ctcgaagga gataatgaa aattgaggt gcctcaaaa 
      tttgcatta gagaaattc caaagataa ttgttaagg 
      atgactaca aaccgttag cattttaca taatttata 
      cctattaat ttaacatta attttatat gtttcatat 
      aactgtttg taattaata catgatgta tttacattg 
      aatgaataa attattgta acaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY