Status
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Gene information
General information
TC012798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15040459..15042353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012798
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09515

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012798 RA
      MPLRIHKNK IVFEIFLVA SISLMCFTP LFFYNRTTT 
      TISLIHIED AKHHVPIPA LVSNNKCLI PDFSQMYFQ 
      QFMYSIPKK YTPCNRKNY LTFVTKSEN TATLHVKQQ 
      NGFYPEVSC CYSNISRNF DRVNPDDTI KISKCRKFT 
      KNVTLATDF VLVKCDTGS VQYKQVHAA ITAKKTTKN 
      SDIWPPPFS VLILGIDKI SRLSFIRNF PKTRHYLTS 
      NNKWVSLNG YAKVSDDTF TNILALLSG CKTMECDLI 
      WSEFEKYNY TTAFGEDDV WSKTFTFHK QLTDYYFRP 
      YFIANEKYP TVELNKMFP CPDRETPFE RLLDLAKDF 
      SVTFKNHPS FALFWIKTA TVGTFPKLD YSIRALFRH 
      LNRKGVFAN TFVFFLSDQ GRREGKNEF TNVGWLEEK 
      LPFVYVLVP KKFQEIYSE EFRFLKLKQ NELTTPFDL 
      YMTLQDIMV LTQVNLDPK PSQSCPTCE SLFMDVTEK 
      SCLDSEIEN RSCPCDGFV LTNLDDLVV RNASLFIVN 
      KVNEFVDQR MCRSYELRK VLSSSASWS SGYKKLLIS 
      VQTYPYSVF EAFVTYVDK VFELDGEIK RLDFAIVRC 
      VSDSFLKKY CVCKGK
    CDS sequence
    • >TC012798 RA
      atgcctctc cgaatccac aaaaacaaa attgtgttt 
      gaaatattc ctggtggct agtatctcc ctaatgtgc 
      ttcaccccc ttatttttc tacaacaga accaccacc 
      acaatatct ctgatacat atcgaagac gccaaacac 
      cacgttccg atcccagca cttgtctct aacaacaag 
      tgtttgatt ccggacttc tcccaaatg tatttccaa 
      caattcatg tactcaatc cccaagaaa tacacaccg 
      tgcaaccga aaaaactac ctcactttt gtgacaaaa 
      agcgaaaat acggccact ctgcacgtc aagcaacaa 
      aatgggttt taccccgaa gtttcctgc tgttattcg 
      aacatttca cgaaacttt gaccgagta aatcccgac 
      gataccatc aaaatttcc aaatgtcgg aagtttacg 
      aaaaatgtg actttggcc actgacttc gtcttggtc 
      aagtgtgac actggttcg gtacaatac aaacaagtc 
      catgctgcg atcacggcc aaaaaaacg accaaaaat 
      tccgacatt tggccacca cctttcagt gtcttgatt 
      ctaggaatc gacaagatt tcgcgccta agtttcatc 
      cggaatttt ccaaaaacc cgtcattat ctaacgtcg 
      aacaataag tgggtttct ttgaacggc tacgcgaaa 
      gtgagcgac gacactttc accaacatt ttggccctt 
      ttaagcggt tgcaaaacc atggagtgt gatttaatt 
      tggtccgag ttcgaaaaa tacaactac accacagcg 
      ttcggtgaa gatgacgtg tggtcaaaa actttcacg 
      ttccacaaa caactcaca gattactac ttccggccc 
      tattttatc gcaaacgaa aaataccca accgtggaa 
      ctcaataaa atgtttccg tgtccagac cgcgaaacg 
      ccctttgaa cgcctcctg gacctggcc aaagacttt 
      agtgtcacg ttcaaaaac cacccaagt ttcgccctt 
      ttctggatc aaaacggca actgtggga accttcccg 
      aagctggac tactcgatc cgggcactt ttccggcac 
      ctgaaccgg aaaggcgtg ttcgcgaac acgttcgtg 
      ttcttcctg agcgatcaa gggaggcgt gaggggaaa 
      aacgagttt acaaatgtg ggctggttg gaggaaaaa 
      ctgcctttc gtttatgtt ctagtcccg aaaaaattc 
      caagagatt tattccgaa gagttcagg tttttgaaa 
      cttaagcaa aacgagtta accactccg tttgatctg 
      tacatgact ttgcaagac attatggtg ctgactcag 
      gtgaatctg gatccgaaa ccgagtcag agttgtccg 
      acgtgtgag tcgcttttt atggatgtt acggaaaag 
      tcgtgtctg gattcggaa attgagaat aggtcttgt 
      ccctgtgat gggttcgtt ttgacgaat cttgacgat 
      ttagttgtg aggaacgcc tcacttttt attgtgaat 
      aaagtaaat gagtttgtt gatcagcga atgtgtcgg 
      agttacgag ttgaggaaa gtgttaagt tcgagcgct 
      tcctggagt tcgggttac aagaaatta ctaataagt 
      gtccaaacg tacccttat tcagttttt gaggcgttt 
      gttacgtat gttgataag gtttttgag ctcgatggt 
      gaaattaaa aggctagat tttgccatt gttaggtgt 
      gttagtgat tcgtttttg aaaaaatat tgtgtgtgt 
      aagggtaaa taa
    mRNA sequence
    • >TC012798 RA
      tcgtcaaaa tgcctctcc gaatccaca aaaacaaaa 
      ttgtgtttg aaatattcc tggtggcta gtatctccc 
      taatgtgct tcaccccct tatttttct acaacagaa 
      ccaccacca caatatctc tgatacata tcgaagacg 
      ccaaacacc acgttccga tcccagcac ttgtctcta 
      acaacaagt gtttgattc cggacttct cccaaatgt 
      atttccaac aattcatgt actcaatcc ccaagaaat 
      acacaccgt gcaaccgaa aaaactacc tcacttttg 
      tgacaaaaa gcgaaaata cggccactc tgcacgtca 
      agcaacaaa atgggtttt accccgaag tttcctgct 
      gttattcga acatttcac gaaactttg accgagtaa 
      atcccgacg ataccatca aaatttcca aatgtcgga 
      agtttacga aaaatgtga ctttggcca ctgacttcg 
      tcttggtca agtgtgaca ctggttcgg tacaataca 
      aacaagtcc atgctgcga tcacggcca aaaaaacga 
      ccaaaaatt ccgacattt ggccaccac ctttcagtg 
      tcttgattc taggaatcg acaagattt cgcgcctaa 
      gtttcatcc ggaattttc caaaaaccc gtcattatc 
      taacgtcga acaataagt gggtttctt tgaacggct 
      acgcgaaag tgagcgacg acactttca ccaacattt 
      tggcccttt taagcggtt gcaaaacca tggagtgtg 
      atttaattt ggtccgagt tcgaaaaat acaactaca 
      ccacagcgt tcggtgaag atgacgtgt ggtcaaaaa 
      ctttcacgt tccacaaac aactcacag attactact 
      tccggccct attttatcg caaacgaaa aatacccaa 
      ccgtggaac tcaataaaa tgtttccgt gtccagacc 
      gcgaaacgc cctttgaac gcctcctgg acctggcca 
      aagacttta gtgtcacgt tcaaaaacc acccaagtt 
      tcgcccttt tctggatca aaacggcaa ctgtgggaa 
      ccttcccga agctggact actcgatcc gggcacttt 
      tccggcacc tgaaccgga aaggcgtgt tcgcgaaca 
      cgttcgtgt tcttcctga gcgatcaag ggaggcgtg 
      aggggaaaa acgagttta caaatgtgg gctggttgg 
      aggaaaaac tgcctttcg tttatgttc tagtcccga 
      aaaaattcc aagagattt attccgaag agttcaggt 
      ttttgaaac ttaagcaaa acgagttaa ccactccgt 
      ttgatctgt acatgactt tgcaagaca ttatggtgc 
      tgactcagg tgaatctgg atccgaaac cgagtcaga 
      gttgtccga cgtgtgagt cgcttttta tggatgtta 
      cggaaaagt cgtgtctgg attcggaaa ttgagaata 
      ggtcttgtc cctgtgatg ggttcgttt tgacgaatc 
      ttgacgatt tagttgtga ggaacgcct cacttttta 
      ttgtgaata aagtaaatg agtttgttg atcagcgaa 
      tgtgtcgga gttacgagt tgaggaaag tgttaagtt 
      cgagcgctt cctggagtt cgggttaca agaaattac 
      taataagtg tccaaacgt acccttatt cagtttttg 
      aggcgtttg ttacgtatg ttgataagg tttttgagc 
      tcgatggtg aaattaaaa ggctagatt ttgccattg 
      ttaggtgtg ttagtgatt cgtttttga aaaaatatt 
      gtgtgtgta agggtaaat aaaacgata aaattataa 
      atgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY