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Gene information
General information
TC012795 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15017709..15020096
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012795
    iBeetle screen iB_09514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012795 RA
      MFRIFLTIL CLLFTETLQ ETYILNNEK CKIPDLEIL 
      NEDVRDIIQ PNPYIPCNK TELLTHVTK NGPKFELHL 
      REDLFPQYF TQNITCCYS YVTRNGSVE LPDVGISIS 
      PCVPFHKKI VLEQDIVKV VCRDNNQSV VYENVHVTI 
      KPSKPLKNT SQPFSVLFV VVDSISRLN FHRTMPETA 
      NFLKKRNFI EMKAYSKVN DNTFPNCMA LLTGFSLSQ 
      SYQHCNPHK KSALDNCPM IWKDFHQHG YTTAYAEDN 
      SRIATFNYL KKGFTNPPT DFYFKPYME ATETLRIQH 
      RHSMPFCTG PESAGERIL NLAKDFAIT FKHNPSFGI 
      FWMNTFSHD DVNAPFTMD AKLSDFFRD LDEAGVLDE 
      SFVFLLSDH GIRFGPMVG TNSGWLEGR MPLNMISVP 
      AKFKREFGR EYENLKQNS GKLTNTYDM FMTLQHILT 
      LGGVRHEVR PSQGCPNCV SLFDPIPDR SCQEAAIPY 
      AWCPCFGRL KTLNPFDVQ SAITYTLSK AKSEILAQC 
      DIDNLDPHV DSVSVSEPD HEKNQILFV VFHVESAIG 
      LATVKFKDV VTEKSLFYF RFIYLYNIC KQDNS
    CDS sequence
    • >TC012795 RA
      atgttccgt attttttta acaattttg tgtcttctg 
      tttaccgaa acattgcaa gaaacatac attttaaac 
      aacgagaaa tgtaaaatc ccagatttg gaaattttg 
      aacgaagat gttagagat attatacag ccaaatcct 
      tacatccct tgcaacaaa accgaactt ttgacccat 
      gtgaccaaa aacggaccc aaatttgag ttacaccta 
      agagaggat ttatttcct caatacttc actcaaaac 
      attacgtgt tgttactcg tacgtaacg agaaacgga 
      tcagttgaa ctccctgat gttggtatc agtatttca 
      ccgtgtgtc ccttttcac aagaagata gttttagag 
      caagatata gttaaagtc gtttgtcgc gataataat 
      caatcagtg gtttacgaa aacgtgcat gttactatc 
      aaaccctca aaaccactc aaaaacacg tcccaacca 
      ttcagcgtt ttgtttgta gtagttgac agtatttct 
      cgcctcaat ttccaccga acaatgcca gaaaccgca 
      aatttcctc aaaaaacga aactttatc gaaatgaaa 
      gcctactca aaagtgaac gataacacg ttcccaaat 
      tgcatggcc ctcctgact gggttttcg ctaagccag 
      tcgtaccag cactgcaac ccgcacaag aaaagtgcc 
      ctagacaac tgtcccatg atctggaag gacttccat 
      caacatggg tacaccaca gcctacgcc gaagacaac 
      tcacgcatt gctaccttc aactacctc aaaaaaggc 
      ttcacgaac ccaccaaca gacttctac ttcaaaccc 
      tacatggag gccaccgaa accctcaga atccagcac 
      cgacattcc atgccattc tgcactggc cctgaaagc 
      gccggtgaa cgcatcctg aacctagcc aaagatttt 
      gccatcacg tttaaacac aatcccagt tttggcatt 
      ttctggatg aacactttc agtcatgac gatgtcaat 
      gcgccattc accatggac gcgaaactg agcgatttt 
      ttccgggat ttggacgag gctggggtt ttggacgaa 
      agcttcgtt tttttgtta agtgaccac gggattaga 
      tttggccca atggtggga accaacagc gggtggctg 
      gagggacga atgccactg aacatgatc tcagttccg 
      gcaaaattc aagcgggag tttggacgc gagtacgag 
      aatttgaaa caaaactcg gggaaatta acaaacact 
      tatgacatg ttcatgact ttgcaacac attttaaca 
      ctggggggc gtccggcat gaagttagg ccgagtcaa 
      gggtgtccc aactgtgtt tcgcttttt gacccaatt 
      cccgatagg tcctgccag gaggcggcc attccctac 
      gcatggtgt ccctgtttt gggcgtttg aaaacactt 
      aatcctttc gatgttcaa agtgcgatc acgtacaca 
      ctgtccaag gcaaaaagc gagattttg gcacagtgt 
      gatattgat aatttggac cctcatgtc gattcagtc 
      agtgtttct gaaccggac catgaaaaa aatcagata 
      cttttcgtg gtctttcat gtggaaagc gccattggt 
      ttggcaaca gtcaaattc aaagatgta gtcacggaa 
      aaatcgtta ttttatttc aggtttatt tatttgtac 
      aatatttgc aagcaagat aattcttga
    mRNA sequence
    • >TC012795 RA
      gtggtcaca aaatgttcc gtatttttt taacaattt 
      tgtgtcttc tgtttaccg aaacattgc aagaaacat 
      acattttaa acaacgaga aatgtaaaa tcccagatt 
      tggaaattt tgaacgaag atgttagag atattatac 
      agccaaatc cttacatcc cttgcaaca aaaccgaac 
      ttttgaccc atgtgacca aaaacggac ccaaatttg 
      agttacacc taagagagg atttatttc ctcaatact 
      tcactcaaa acattacgt gttgttact cgtacgtaa 
      cgagaaacg gatcagttg aactccctg atgttggta 
      tcagtattt caccgtgtg tcccttttc acaagaaga 
      tagttttag agcaagata tagttaaag tcgtttgtc 
      gcgataata atcaatcag tggtttacg aaaacgtgc 
      atgttacta tcaaaccct caaaaccac tcaaaaaca 
      cgtcccaac cattcagcg ttttgtttg tagtagttg 
      acagtattt ctcgcctca atttccacc gaacaatgc 
      cagaaaccg caaatttcc tcaaaaaac gaaacttta 
      tcgaaatga aagcctact caaaagtga acgataaca 
      cgttcccaa attgcatgg ccctcctga ctgggtttt 
      cgctaagcc agtcgtacc agcactgca acccgcaca 
      agaaaagtg ccctagaca actgtccca tgatctgga 
      aggacttcc atcaacatg ggtacacca cagcctacg 
      ccgaagaca actcacgca ttgctacct tcaactacc 
      tcaaaaaag gcttcacga acccaccaa cagacttct 
      acttcaaac cctacatgg aggccaccg aaaccctca 
      gaatccagc accgacatt ccatgccat tctgcactg 
      gccctgaaa gcgccggtg aacgcatcc tgaacctag 
      ccaaagatt ttgccatca cgtttaaac acaatccca 
      gttttggca ttttctgga tgaacactt tcagtcatg 
      acgatgtca atgcgccat tcaccatgg acgcgaaac 
      tgagcgatt ttttccggg atttggacg aggctgggg 
      ttttggacg aaagcttcg tttttttgt taagtgacc 
      acgggatta gatttggcc caatggtgg gaaccaaca 
      gcgggtggc tggagggac gaatgccac tgaacatga 
      tctcagttc cggcaaaat tcaagcggg agtttggac 
      gcgagtacg agaatttga aacaaaact cggggaaat 
      taacaaaca cttatgaca tgttcatga ctttgcaac 
      acattttaa cactggggg gcgtccggc atgaagtta 
      ggccgagtc aagggtgtc ccaactgtg tttcgcttt 
      ttgacccaa ttcccgata ggtcctgcc aggaggcgg 
      ccattccct acgcatggt gtccctgtt ttgggcgtt 
      tgaaaacac ttaatcctt tcgatgttc aaagtgcga 
      tcacgtaca cactgtcca aggcaaaaa gcgagattt 
      tggcacagt gtgatattg ataatttgg accctcatg 
      tcgattcag tcagtgttt ctgaaccgg accatgaaa 
      aaaatcaga tacttttcg tggtctttc atgtggaaa 
      gcgccattg gtttggcaa cagtcaaat tcaaagatg 
      tagtcacgg aaaaatcgt tattttatt tcaggttta 
      tttatttgt acaatattt gcaagcaag ataattctt 
      gaataattt aaacagata tttatccaa cattgcacc 
      catctgtcc tgcaatatt taatcgact ggtaaccac 
      cgtttttcc gcaccaaat cgtcaaact tatcgaacg 
      ttattatac gattttgct caaaagggg cgcaccctt 
      tgtacacct catctacaa gtccaaatc aatcaatca 
      tttttcacc atctagtgt gttacttct aaatttcag 
      aatgtaaca ataatcagt acactcgtc ataaacggt 
      tcgtaaagg acaagaagc gtaacatct tatgcagcg 
      aaaccctaa aagcacgat ttattacgt ttcgtcagt 
      cgggactca ccttcagaa gggcacaag actggagca 
      agtccaagg agaatcaac attactttc atttcgacc 
      gaactgagc gaaaaattg attgcgttt gcgactttg 
      aattgaatt gttttgact tgaacttgg gtgatttta 
      tgacccata aaaatctgt ttttctctc tgtgttaac 
      caccaagac acggttttc tccatttgt ttataattt 
      aagtaccac aataaagat ttaataaaa cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY